Transcriptome.h

#ifndef CODE_Transcriptome
#define CODE_Transcriptome

#include <set>

#include "IncludeDefine.h"
#include "Parameters.h"
#include "Transcript.h"
#include "Quantifications.h"

class Transcriptome {
public:

    //methods:
    Transcriptome (Parameters &Pin); //create transcriptome structure, load and initialize parameters
    uint32 quantAlign (Transcript &aG, Transcript *aTall, vector<uint32> &readTranscripts, set<uint32> &readTrGenes);//transform coordinates for all aligns from genomic in RA to transcriptomic in RAtr
    void geneCountsAddAlign(uint nA, Transcript **aAll, vector<int32> &gene1); //add one alignment to gene counts
    void quantsAllocate(); //allocate quants structure
    void quantsOutput(); //output quantification files

    string trInfoDir;

    vector <string> trID, geID; //transcript/gene IDs
    uint32 nTr, nGe; //number of transcript/genes

    uint *trS, *trE, *trEmax; //transcripts start,end,end-max

    uint32 nEx; //number of exons
    uint16 *trExN; //number of exons per transcript
    uint32 *trExI; //index of the first exon for each transcript in exSE
    uint8 *trStr; //transcript strand
    uint32 *exSE; //exons start/end
    uint32 *exLenCum; //cumulative length of previous exons
    uint32 *trGene; //transcript to gene correspondence

    struct {//exon-gene structure for GeneCounts
       uint64 nEx;//number of exons/genes
       uint64 *s,*e, *eMax;  //exon start/end
       uint8  *str;   //strand
       uint32 *g, *t; //gene/transcript IDs
    } exG;

    Quantifications *quants;

private:
    Parameters &P; //normal "genomic" parameters

};

#endif