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Improvement: Add bitscore and query scaffold coverage to taxonomic classification output #77

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DennisSchmitz opened this issue Jul 24, 2019 · 0 comments
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@DennisSchmitz
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Sometimes the taxonomic classification is based on only a very small (<200nt), but unique, hit against a certain taxa. Then scaffold then gets assigned the taxa of that very small hit. Nevertheless, when you then do a protein blast of that same scaffold you get a much better (longer) hit against another taxa, clearly proving that is a wrong annotation.

Since it is not feasible to do a tBLASTX for everything, a solution would be to add a metric that denotes the percentage of the scaffold that is covered by the alignment and to include the BLAST bitscore. That way, people can filter for this. We might even flag these classifications as untrustworthy.

This feature is not included in the launch paper, hence it is scheduled for v1.1 (the first release post publication).

@DennisSchmitz DennisSchmitz added the enhancement New feature or request label Jul 24, 2019
@DennisSchmitz DennisSchmitz added this to the 1.1 milestone Jul 24, 2019
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