diff --git a/keyboards/abatskeyboardclub/nayeon/config.h b/keyboards/abatskeyboardclub/nayeon/config.h
index 7fedf4dce12d..f225f555f9b8 100644
--- a/keyboards/abatskeyboardclub/nayeon/config.h
+++ b/keyboards/abatskeyboardclub/nayeon/config.h
@@ -17,7 +17,5 @@ along with this program.  If not, see <http://www.gnu.org/licenses/>.
 
 #pragma once
 
-
 /* RGB Matrix setup */
 #define RGB_MATRIX_LED_COUNT 2
-#define WS2812_PIO_USE_PIO1 // Force the usage of PIO1 peripheral, by default the WS2812 implementation uses the PIO0 peripheral
diff --git a/keyboards/cannonkeys/bastion60/config.h b/keyboards/cannonkeys/bastion60/config.h
index 206ca9cf564f..f410fb532578 100644
--- a/keyboards/cannonkeys/bastion60/config.h
+++ b/keyboards/cannonkeys/bastion60/config.h
@@ -10,5 +10,3 @@
 
 #define BACKLIGHT_PWM_DRIVER PWMD3
 #define BACKLIGHT_PWM_CHANNEL RP2040_PWM_CHANNEL_A
-
-#define WS2812_PIO_USE_PIO1
diff --git a/keyboards/cannonkeys/bastion65/config.h b/keyboards/cannonkeys/bastion65/config.h
index 0b93d1434be9..640aa331343d 100644
--- a/keyboards/cannonkeys/bastion65/config.h
+++ b/keyboards/cannonkeys/bastion65/config.h
@@ -24,5 +24,3 @@ along with this program.  If not, see <http://www.gnu.org/licenses/>.
 
 #define BACKLIGHT_PWM_DRIVER PWMD3
 #define BACKLIGHT_PWM_CHANNEL RP2040_PWM_CHANNEL_A
-
-#define WS2812_PIO_USE_PIO1
diff --git a/keyboards/cannonkeys/bastion75/config.h b/keyboards/cannonkeys/bastion75/config.h
index 0b93d1434be9..640aa331343d 100644
--- a/keyboards/cannonkeys/bastion75/config.h
+++ b/keyboards/cannonkeys/bastion75/config.h
@@ -24,5 +24,3 @@ along with this program.  If not, see <http://www.gnu.org/licenses/>.
 
 #define BACKLIGHT_PWM_DRIVER PWMD3
 #define BACKLIGHT_PWM_CHANNEL RP2040_PWM_CHANNEL_A
-
-#define WS2812_PIO_USE_PIO1
diff --git a/keyboards/controllerworks/city42/config.h b/keyboards/controllerworks/city42/config.h
index 4e161532f2ac..7633a27c4055 100644
--- a/keyboards/controllerworks/city42/config.h
+++ b/keyboards/controllerworks/city42/config.h
@@ -17,7 +17,6 @@
 
 #pragma once
 
-#define WS2812_PIO_USE_PIO1
 #define RGB_MATRIX_LED_COUNT 42
 
 #define SPI_SCK_PIN GP18
diff --git a/keyboards/jels/jels60/v2/config.h b/keyboards/jels/jels60/v2/config.h
index 02d9619a301c..c9c1ba2c0e2c 100644
--- a/keyboards/jels/jels60/v2/config.h
+++ b/keyboards/jels/jels60/v2/config.h
@@ -16,7 +16,6 @@
 
 #pragma once
 
-#define WS2812_PIO_USE_PIO1
 #define RGBLIGHT_DEFAULT_VAL 0
 
 /* Mechanical locking support. Use KC_LCAP, KC_LNUM or KC_LSCR instead in keymap */
diff --git a/keyboards/kwstudio/scorpio/config.h b/keyboards/kwstudio/scorpio/config.h
deleted file mode 100644
index 2f136dd0011f..000000000000
--- a/keyboards/kwstudio/scorpio/config.h
+++ /dev/null
@@ -1,20 +0,0 @@
-/* Copyright 2023 kwstudio
- *
- * This program is free software: you can redistribute it and/or modify
- * it under the terms of the GNU General Public License as published by
- * the Free Software Foundation, either version 2 of the License, or
- * (at your option) any later version.
- *
- * This program is distributed in the hope that it will be useful,
- * but WITHOUT ANY WARRANTY; without even the implied warranty of
- * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
- * GNU General Public License for more details.
- *
- * You should have received a copy of the GNU General Public License
- * along with this program.  If not, see <http://www.gnu.org/licenses/>.
- */
-
-#pragma once
-
-#define WS2812_PIO_USE_PIO1
-
diff --git a/keyboards/lxxt/config.h b/keyboards/lxxt/config.h
deleted file mode 100644
index 03ef56622c0c..000000000000
--- a/keyboards/lxxt/config.h
+++ /dev/null
@@ -1,19 +0,0 @@
-/* Copyright 2023 DeskDaily
- *
- * This program is free software: you can redistribute it and/or modify
- * it under the terms of the GNU General Public License as published by
- * the Free Software Foundation, either version 2 of the License, or
- * (at your option) any later version.
- *
- * This program is distributed in the hope that it will be useful,
- * but WITHOUT ANY WARRANTY; without even the implied warranty of
- * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
- * GNU General Public License for more details.
- *
- * You should have received a copy of the GNU General Public License
- * along with this program.  If not, see <http://www.gnu.org/licenses/>.
- */
-
-#pragma once
-
-#define WS2812_PIO_USE_PIO1
\ No newline at end of file
diff --git a/keyboards/saevus/cor_tkl/config.h b/keyboards/saevus/cor_tkl/config.h
index 7fedf4dce12d..5a086eb76827 100644
--- a/keyboards/saevus/cor_tkl/config.h
+++ b/keyboards/saevus/cor_tkl/config.h
@@ -20,4 +20,3 @@ along with this program.  If not, see <http://www.gnu.org/licenses/>.
 
 /* RGB Matrix setup */
 #define RGB_MATRIX_LED_COUNT 2
-#define WS2812_PIO_USE_PIO1 // Force the usage of PIO1 peripheral, by default the WS2812 implementation uses the PIO0 peripheral
diff --git a/keyboards/skeletonkbd/frost68/config.h b/keyboards/skeletonkbd/frost68/config.h
index de768e745bae..4300462691bd 100644
--- a/keyboards/skeletonkbd/frost68/config.h
+++ b/keyboards/skeletonkbd/frost68/config.h
@@ -3,7 +3,6 @@
 
 #pragma once
 
-#define WS2812_PIO_USE_PIO1
 #define RGB_MATRIX_LED_COUNT 68
 
 #define RGB_MATRIX_SLEEP
diff --git a/keyboards/smoll/pw88/config.h b/keyboards/smoll/pw88/config.h
deleted file mode 100644
index f655b9252728..000000000000
--- a/keyboards/smoll/pw88/config.h
+++ /dev/null
@@ -1,21 +0,0 @@
-/*
-Copyright 2023 Matthijs Muller
-
-This program is free software: you can redistribute it and/or modify
-it under the terms of the GNU General Public License as published by
-the Free Software Foundation, either version 2 of the License, or
-(at your option) any later version.
-
-This program is distributed in the hope that it will be useful,
-but WITHOUT ANY WARRANTY; without even the implied warranty of
-MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
-GNU General Public License for more details.
-
-You should have received a copy of the GNU General Public License
-along with this program.  If not, see <http://www.gnu.org/licenses/>.
-*/
-
-
-#pragma once
-
-#define WS2812_PIO_USE_PIO1 // Force the usage of PIO1 peripheral, by default the WS2812 implementation uses the PIO0 peripheral
diff --git a/keyboards/tweetydabird/chameleon/config.h b/keyboards/tweetydabird/chameleon/config.h
deleted file mode 100644
index ba31336014e8..000000000000
--- a/keyboards/tweetydabird/chameleon/config.h
+++ /dev/null
@@ -1,6 +0,0 @@
-// Copyright 2023 Markus Knutsson (@TweetyDaBird)
-// SPDX-License-Identifier: GPL-2.0-or-later
-
-#pragma once
-
-#define WS2812_PIO_USE_PIO1 // Force the usage of PIO1 peripheral, by default the WS2812 implementation uses the PIO0 peripheral