EGFR MET Barcoded Amplicons Validation

Instrument:  PacBio RS
Chemistry:  C2
Enzyme: C2

Summary

This dataset consists of two SMRT Cells with two movies each in bas.h5 format. Each target amplicon in the EGFR-MET Fluidigm Array is barcoded with a different barcode pair from the standard PacBio 16bp barcode set (48 pairs).

Validation for barcodes can be done by resequencing these data against the EGFR_MET reference dataset. Each barcode should map to only a single target amplicon.

Related Topics

• Detailed tutorial using the EGFR-MET dataset
• General overview on Barcoding

Download Datasets

Secondary Analysis Data

• The data can be downloaded here.