Analyze Oyster gonad Histo slides

[sr320]

No description provided.

[sr320]

coordinate with @mattgeorgephd for details.

[mattgeorgephd]

Hi @drlawson. I placed the samples on the shelf above the desk in the back of FTR 209. Here is a picture of the box and a slide diagram.

[mattgeorgephd]

@drlawson

Here is the Shelly's notebook entry outlining how she classified gonad development in geoduck.

Here is the paper from Stan Allen's group that proposes a new classification system for triploid oysters.

[drlawson]

@mattgeorgephd https://drlawson.github.io/Updated-Oyster-Pics-for-Matt/ here is my notebook entry containing important images, links to all the images I took, and the link to the staging sheet for the samples. Let me know what you think/if you need anything else from me :)

[mattgeorgephd]

@drlawson Thanks for your help on this! Your analysis and notebook entry were great!

We might have some more samples sectioned in the future - I'll keep you posted when/if we get samples back.

[mattgeorgephd]

Hey @drlawson! We have received a second batch of histology slides (n=37) for analysis if you are up for it. The samples are oyster gonad, in the same orientation as the previous set.

Here is a picture and slide diagram. I will leave them in FTR 209 on the shelving in the back, right-hand corner.

[drlawson]

awesome! I'll swing by to pick these up friday or saturday :) do either of you @mattgeorgephd @sr320 know if the microscope I was using before is available for me to also take home? If not I'll write about it in the slack real quick. thanks!

[drlawson]

@mattgeorgephd just picked up the boxes and microscope - I left the box with the old samples in the lab :) I’ll start working on this this week!

[mattgeorgephd]

@drlawson I've added you as a collaborator on the NOPP-gigas-ploidy-temp repo. Please work within the histology folder in the 202107_EXP2 folder.

[drlawson]

hi @mattgeorgephd, I'm working on staging those samples. Instead of D and T like last time the samples have the letters N, M, R, and X. What do those signify? Which are diploid and which are triploid? I ask because I was using a slightly different staging method for triploids last time :) Thanks!

[mattgeorgephd]

@drlawson sorry for the confusion, here is the key.

M, N = diploid
R, X = triploid

[sr320]

@mattgeorgephd is this completed?