diff --git a/.changeset/swift-penguins-help.md b/.changeset/swift-penguins-help.md
new file mode 100644
index 000000000000..09588cc8771a
--- /dev/null
+++ b/.changeset/swift-penguins-help.md
@@ -0,0 +1,5 @@
+---
+'@rocket.chat/meteor': major
+---
+
+Change the E2EE setting - "Access unencrypted content in encrypted rooms" default value, making the current behavior not allow to send unencrypted messages in end-to-end encrypted channels.
diff --git a/apps/meteor/server/settings/e2e.ts b/apps/meteor/server/settings/e2e.ts
index dfaabdc3f237..8d80ad5b8c18 100644
--- a/apps/meteor/server/settings/e2e.ts
+++ b/apps/meteor/server/settings/e2e.ts
@@ -10,7 +10,7 @@ export const createE2ESettings = () =>
 			alert: 'E2E_Enable_alert',
 		});
 
-		await this.add('E2E_Allow_Unencrypted_Messages', true, {
+		await this.add('E2E_Allow_Unencrypted_Messages', false, {
 			type: 'boolean',
 			public: true,
 			enableQuery: { _id: 'E2E_Enable', value: true },
diff --git a/apps/meteor/server/startup/migrations/index.ts b/apps/meteor/server/startup/migrations/index.ts
index 6bbe89a1726b..c77d750b25c4 100644
--- a/apps/meteor/server/startup/migrations/index.ts
+++ b/apps/meteor/server/startup/migrations/index.ts
@@ -46,5 +46,6 @@ import './v310';
 import './v311';
 import './v312';
 import './v313';
+import './v314';
 
 export * from './xrun';
diff --git a/apps/meteor/server/startup/migrations/v314.ts b/apps/meteor/server/startup/migrations/v314.ts
new file mode 100644
index 000000000000..0b46cc42838a
--- /dev/null
+++ b/apps/meteor/server/startup/migrations/v314.ts
@@ -0,0 +1,21 @@
+import { Settings } from '@rocket.chat/models';
+
+import { addMigration } from '../../lib/migrations';
+
+addMigration({
+	version: 314,
+	name: 'Update default behavior of E2E_Allow_Unencrypted_Messages setting, to not allow un-encrypted messages by default.',
+	async up() {
+		await Settings.updateOne(
+			{
+				_id: 'E2E_Allow_Unencrypted_Messages',
+			},
+			{
+				$set: {
+					value: false,
+					packageValue: false,
+				},
+			},
+		);
+	},
+});