diff --git a/src/language/builtins/safe-ds-annotations.ts b/src/language/builtins/safe-ds-annotations.ts
index e7e6d1a24..aeabecd95 100644
--- a/src/language/builtins/safe-ds-annotations.ts
+++ b/src/language/builtins/safe-ds-annotations.ts
@@ -31,6 +31,14 @@ export class SafeDsAnnotations extends SafeDsModuleMembers<SdsAnnotation> {
         return this.getAnnotation(CORE_ANNOTATIONS_URI, 'Expert');
     }
 
+    isRepeatable(node: SdsAnnotation | undefined): boolean {
+        return this.hasAnnotationCallOf(node, this.Repeatable);
+    }
+
+    private get Repeatable(): SdsAnnotation | undefined {
+        return this.getAnnotation(CORE_ANNOTATIONS_URI, 'Repeatable');
+    }
+
     private hasAnnotationCallOf(node: SdsAnnotatedObject | undefined, expected: SdsAnnotation | undefined): boolean {
         return annotationCallsOrEmpty(node).some((it) => {
             const actual = it.annotation?.ref;
diff --git a/src/language/validation/builtins/repeatable.ts b/src/language/validation/builtins/repeatable.ts
new file mode 100644
index 000000000..a4cf518bf
--- /dev/null
+++ b/src/language/validation/builtins/repeatable.ts
@@ -0,0 +1,22 @@
+import { ValidationAcceptor } from 'langium';
+import { SdsAnnotatedObject } from '../../generated/ast.js';
+import { SafeDsServices } from '../../safe-ds-module.js';
+import { annotationCallsOrEmpty } from '../../helpers/nodeProperties.js';
+import { duplicatesBy } from '../../helpers/collectionUtils.js';
+
+export const CODE_ANNOTATION_NOT_REPEATABLE = 'annotation/not-repeatable';
+
+export const singleUseAnnotationsMustNotBeRepeated =
+    (services: SafeDsServices) => (node: SdsAnnotatedObject, accept: ValidationAcceptor) => {
+        const callsOfSingleUseAnnotations = annotationCallsOrEmpty(node).filter((it) => {
+            const annotation = it.annotation?.ref;
+            return annotation && !services.builtins.Annotations.isRepeatable(annotation);
+        });
+
+        for (const duplicate of duplicatesBy(callsOfSingleUseAnnotations, (it) => it.annotation?.ref)) {
+            accept('error', `The annotation '${duplicate.annotation.$refText}' is not repeatable.`, {
+                node: duplicate,
+                code: CODE_ANNOTATION_NOT_REPEATABLE,
+            });
+        }
+    };
diff --git a/src/language/validation/safe-ds-validator.ts b/src/language/validation/safe-ds-validator.ts
index b4d527410..2cdf0ad7a 100644
--- a/src/language/validation/safe-ds-validator.ts
+++ b/src/language/validation/safe-ds-validator.ts
@@ -76,6 +76,7 @@ import {
 } from './other/declarations/annotationCalls.js';
 import { memberAccessMustBeNullSafeIfReceiverIsNullable } from './other/expressions/memberAccesses.js';
 import { importPackageMustExist, importPackageShouldNotBeEmpty } from './other/imports.js';
+import { singleUseAnnotationsMustNotBeRepeated } from './builtins/repeatable.js';
 
 /**
  * Register custom validation checks.
@@ -98,6 +99,7 @@ export const registerValidationChecks = function (services: SafeDsServices) {
             annotationCallAnnotationShouldNotBeExperimental(services),
             annotationCallArgumentListShouldBeNeeded,
         ],
+        SdsAnnotatedObject: [singleUseAnnotationsMustNotBeRepeated(services)],
         SdsArgument: [
             argumentCorrespondingParameterShouldNotBeDeprecated(services),
             argumentCorrespondingParameterShouldNotBeExperimental(services),
diff --git a/tests/resources/validation/builtins/annotations/repeatable/main.sdstest b/tests/resources/validation/builtins/annotations/repeatable/main.sdstest
new file mode 100644
index 000000000..eb642f8e8
--- /dev/null
+++ b/tests/resources/validation/builtins/annotations/repeatable/main.sdstest
@@ -0,0 +1,24 @@
+package tests.validation.builtins.annotations.repeatable
+
+annotation SingleUse
+
+@Repeatable
+annotation MultiUse
+
+// $TEST$ no error r"The annotation '\w*' is not repeatable\."
+»@SingleUse«
+// $TEST$ no error r"The annotation '\w*' is not repeatable\."
+»@MultiUse«
+// $TEST$ no error r"The annotation '\w*' is not repeatable\."
+»@MultiUse«
+// $TEST$ no error r"The annotation '\w*' is not repeatable\."
+»@UnresolvedAnnotation«
+// $TEST$ no error r"The annotation '\w*' is not repeatable\."
+»@UnresolvedAnnotation«
+class CorrectUse
+
+// $TEST$ no error r"The annotation '\w*' is not repeatable\."
+»@SingleUse«
+// $TEST$ error "The annotation 'SingleUse' is not repeatable."
+»@SingleUse«
+class IncorrectUse
diff --git a/tests/resources/validation/other/statements/assignments/nothing assigned/main.sdstest b/tests/resources/validation/other/statements/assignments/nothing assigned/main.sdstest
index dcd48f85f..3cee2dd40 100644
--- a/tests/resources/validation/other/statements/assignments/nothing assigned/main.sdstest	
+++ b/tests/resources/validation/other/statements/assignments/nothing assigned/main.sdstest	
@@ -58,6 +58,7 @@ segment mySegment() -> (
     // $TEST$ error "No value is assigned to this assignee."
     »val k«, »val l« = unresolved();
 
+
     // $TEST$ error "No value is assigned to this assignee."
     »yield r1« = noResults();
     // $TEST$ no error "No value is assigned to this assignee."