diff --git a/packages/components/CHANGELOG.md b/packages/components/CHANGELOG.md
index df9fef03d219b7..c743ebb3dc8a5c 100644
--- a/packages/components/CHANGELOG.md
+++ b/packages/components/CHANGELOG.md
@@ -7,6 +7,7 @@
 -   Removed the deprecated `position` and `menuLabel` from the `DropdownMenu` component ([#34537](https://github.com/WordPress/gutenberg/pull/34537)).
 -   Removed the deprecated `onClickOutside` prop from the `Popover` component ([#34537](https://github.com/WordPress/gutenberg/pull/34537)).
 -   Changed `RangeControl` component to not apply `shiftStep` to inputs from its `<input type="range"/>` ([35020](https://github.com/WordPress/gutenberg/pull/35020)).
+-   Removed `isAction` prop from `Item`. The component will now rely on `onClick` to render as a `button` ([35152](https://github.com/WordPress/gutenberg/pull/35152)).
 
 ### New Feature
 
diff --git a/packages/components/src/item-group/item/README.md b/packages/components/src/item-group/item/README.md
index dc5ac92fca4ba3..093d9fc192f6d7 100644
--- a/packages/components/src/item-group/item/README.md
+++ b/packages/components/src/item-group/item/README.md
@@ -29,12 +29,11 @@ function Example() {
 
 ## Props
 
-### `isAction`: `boolean`
+### `onClick`: `React.MouseEventHandler<HTMLDivElement>`
 
-Renders the item as an interactive `button` element.
+Even handler for processing `click` events. When defined, the `Item` component will render as a `button` (unless differently specified via the `as` prop).
 
 - Required: No
-- Default: `false`
 
 ### `size`: `'small' | 'medium' | 'large'`
 
diff --git a/packages/components/src/item-group/item/hook.ts b/packages/components/src/item-group/item/hook.ts
index 2ff7574751098e..d842a29b1eb036 100644
--- a/packages/components/src/item-group/item/hook.ts
+++ b/packages/components/src/item-group/item/hook.ts
@@ -4,6 +4,11 @@
 // eslint-disable-next-line no-restricted-imports
 import type { ElementType } from 'react';
 
+/**
+ * WordPress dependencies
+ */
+import { useMemo } from '@wordpress/element';
+
 /**
  * Internal dependencies
  */
@@ -15,9 +20,9 @@ import type { ItemProps } from '../types';
 
 export function useItem( props: WordPressComponentProps< ItemProps, 'div' > ) {
 	const {
-		isAction = false,
 		as: asProp,
 		className,
+		onClick,
 		role = 'listitem',
 		size: sizeProp,
 		...otherProps
@@ -27,16 +32,24 @@ export function useItem( props: WordPressComponentProps< ItemProps, 'div' > ) {
 
 	const size = sizeProp || contextSize;
 
-	const as = ( asProp || isAction ? 'button' : 'div' ) as ElementType;
+	const as =
+		asProp ||
+		( ( typeof onClick !== 'undefined'
+			? 'button'
+			: 'div' ) as ElementType );
 
 	const cx = useCx();
 
-	const classes = cx(
-		isAction && styles.unstyledButton,
-		styles.itemSizes[ size ] || styles.itemSizes.medium,
-		styles.item,
-		spacedAround && styles.spacedAround,
-		className
+	const classes = useMemo(
+		() =>
+			cx(
+				as === 'button' && styles.unstyledButton,
+				styles.itemSizes[ size ] || styles.itemSizes.medium,
+				styles.item,
+				spacedAround && styles.spacedAround,
+				className
+			),
+		[ as, className, size, spacedAround ]
 	);
 
 	const wrapperClassName = cx( styles.itemWrapper );
@@ -44,6 +57,7 @@ export function useItem( props: WordPressComponentProps< ItemProps, 'div' > ) {
 	return {
 		as,
 		className: classes,
+		onClick,
 		wrapperClassName,
 		role,
 		...otherProps,
diff --git a/packages/components/src/item-group/stories/index.js b/packages/components/src/item-group/stories/index.js
index 30fb1a02150aac..6c0ad5a671ffd7 100644
--- a/packages/components/src/item-group/stories/index.js
+++ b/packages/components/src/item-group/stories/index.js
@@ -56,7 +56,6 @@ export const _default = () => {
 			},
 			PROP_UNSET
 		),
-		isAction: boolean( 'Item 1: isAction', true ),
 	};
 
 	// Do not pass the `size` prop when its value is `undefined`.
@@ -68,16 +67,14 @@ export const _default = () => {
 
 	return (
 		<ItemGroup style={ { width: '350px' } } { ...itemGroupProps }>
-			<Item { ...itemProps } onClick={ () => alert( 'WordPress.org' ) }>
+			<Item { ...itemProps }>Code is Poetry (no click handlers)</Item>
+			<Item onClick={ () => alert( 'WordPress.org' ) }>
 				Code is Poetry — Click me!
 			</Item>
-			<Item isAction onClick={ () => alert( 'WordPress.org' ) }>
+			<Item onClick={ () => alert( 'WordPress.org' ) }>
 				Code is Poetry — Click me!
 			</Item>
-			<Item isAction onClick={ () => alert( 'WordPress.org' ) }>
-				Code is Poetry — Click me!
-			</Item>
-			<Item isAction onClick={ () => alert( 'WordPress.org' ) }>
+			<Item onClick={ () => alert( 'WordPress.org' ) }>
 				Code is Poetry — Click me!
 			</Item>
 		</ItemGroup>
@@ -90,16 +87,14 @@ export const dropdown = () => (
 		trigger={ <Button>Open Popover</Button> }
 	>
 		<ItemGroup style={ { padding: 4 } }>
-			<Item isAction onClick={ () => alert( 'WordPress.org' ) }>
-				Code is Poetry — Click me!
-			</Item>
-			<Item isAction onClick={ () => alert( 'WordPress.org' ) }>
+			<Item>Code is Poetry (no click handlers)</Item>
+			<Item onClick={ () => alert( 'WordPress.org' ) }>
 				Code is Poetry — Click me!
 			</Item>
-			<Item isAction onClick={ () => alert( 'WordPress.org' ) }>
+			<Item onClick={ () => alert( 'WordPress.org' ) }>
 				Code is Poetry — Click me!
 			</Item>
-			<Item isAction onClick={ () => alert( 'WordPress.org' ) }>
+			<Item onClick={ () => alert( 'WordPress.org' ) }>
 				Code is Poetry — Click me!
 			</Item>
 		</ItemGroup>
@@ -141,7 +136,7 @@ export const complexLayouts = () => {
 
 	return (
 		<ItemGroup isBordered isSeparated style={ { width: '250px' } }>
-			<Item isAction onClick={ () => alert( 'Color palette' ) }>
+			<Item onClick={ () => alert( 'Color palette' ) }>
 				<HStack>
 					<FlexBlock>
 						<ZStack isLayered={ false } offset={ -8 }>
@@ -156,7 +151,7 @@ export const complexLayouts = () => {
 				</HStack>
 			</Item>
 
-			<Item isAction onClick={ () => alert( 'Single color setting' ) }>
+			<Item onClick={ () => alert( 'Single color setting' ) }>
 				<HStack justify="flex-start">
 					<FlexItem
 						as={ SimpleColorSwatch }
@@ -169,10 +164,7 @@ export const complexLayouts = () => {
 				</HStack>
 			</Item>
 
-			<Item
-				isAction
-				onClick={ () => alert( 'Single typography setting' ) }
-			>
+			<Item onClick={ () => alert( 'Single typography setting' ) }>
 				<HStack justify="flex-start">
 					<FlexItem>
 						<Icon icon={ typography } size={ 24 } />
diff --git a/packages/components/src/item-group/test/__snapshots__/index.js.snap b/packages/components/src/item-group/test/__snapshots__/index.js.snap
index 4f96fd6f7e6504..4e5ee1d89f53de 100644
--- a/packages/components/src/item-group/test/__snapshots__/index.js.snap
+++ b/packages/components/src/item-group/test/__snapshots__/index.js.snap
@@ -33,28 +33,6 @@ Snapshot Diff:
       </div>
 `;
 
-exports[`ItemGroup Item should render a button with the isAction prop is true 1`] = `
-Snapshot Diff:
-- First value
-+ Second value
-
-  <div
-    class="css-dcjs67-itemWrapper"
-    role="listitem"
-  >
--   <div
--     class="components-item css-gqguxs-View-medium-itemWrapper em57xhy0"
-+   <button
-+     class="components-item css-1t39803-View-unstyledButton-medium-itemWrapper em57xhy0"
-      data-wp-c16t="true"
-      data-wp-component="Item"
-    >
-      Code is poetry
--   </div>
-+   </button>
-  </div>
-`;
-
 exports[`ItemGroup Item should use different amounts of padding depending on the value of the size prop 1`] = `
 Snapshot Diff:
 - First value
diff --git a/packages/components/src/item-group/test/index.js b/packages/components/src/item-group/test/index.js
index 3ae1eb78d4586c..739a02e74435bc 100644
--- a/packages/components/src/item-group/test/index.js
+++ b/packages/components/src/item-group/test/index.js
@@ -1,7 +1,7 @@
 /**
  * External dependencies
  */
-import { render } from '@testing-library/react';
+import { fireEvent, render, screen } from '@testing-library/react';
 
 /**
  * Internal dependencies
@@ -79,18 +79,36 @@ describe( 'ItemGroup', () => {
 	} );
 
 	describe( 'Item', () => {
-		it( 'should render a button with the isAction prop is true', () => {
-			// By default, `isAction` is `false`
-			const { container: normalItem } = render(
-				<Item>Code is poetry</Item>
-			);
-			const { container: actionItem } = render(
-				<Item isAction={ true }>Code is poetry</Item>
+		it( 'should render as a `button` if the `onClick` handler is specified', () => {
+			const spy = jest.fn();
+			render( <Item onClick={ spy }>Code is poetry</Item> );
+
+			const button = screen.getByRole( 'button' );
+
+			expect( button ).toBeInTheDocument();
+
+			fireEvent.click( button );
+
+			expect( spy ).toHaveBeenCalled();
+		} );
+
+		it( 'should give priority to the `as` prop even if the `onClick` handler is specified', () => {
+			const spy = jest.fn();
+			const { rerender } = render(
+				<Item onClick={ spy }>Code is poetry</Item>
 			);
 
-			expect( normalItem.firstChild ).toMatchDiffSnapshot(
-				actionItem.firstChild
+			expect( screen.getByRole( 'button' ) ).toBeInTheDocument();
+			expect( screen.queryByRole( 'label' ) ).not.toBeInTheDocument();
+
+			rerender(
+				<Item as="a" href="#" onClick={ spy }>
+					Code is poetry
+				</Item>
 			);
+
+			expect( screen.queryByRole( 'button' ) ).not.toBeInTheDocument();
+			expect( screen.getByRole( 'link' ) ).toBeInTheDocument();
 		} );
 
 		it( 'should use different amounts of padding depending on the value of the size prop', () => {
diff --git a/packages/components/src/item-group/types.ts b/packages/components/src/item-group/types.ts
index ed07be8a133f17..39aad8c6902c72 100644
--- a/packages/components/src/item-group/types.ts
+++ b/packages/components/src/item-group/types.ts
@@ -32,12 +32,6 @@ export interface ItemGroupProps {
 }
 
 export interface ItemProps {
-	/**
-	 * Renders the item as an interactive `button` element.
-	 *
-	 * @default false
-	 */
-	isAction?: boolean;
 	/**
 	 * Determines the amount of padding within the component.
 	 *
diff --git a/packages/edit-site/src/components/sidebar/global-styles-sidebar.js b/packages/edit-site/src/components/sidebar/global-styles-sidebar.js
index 2d740ec9a8f3e2..5bd826922f1ced 100644
--- a/packages/edit-site/src/components/sidebar/global-styles-sidebar.js
+++ b/packages/edit-site/src/components/sidebar/global-styles-sidebar.js
@@ -206,11 +206,7 @@ function NavigationButton( {
 } ) {
 	const navigator = useNavigator();
 	return (
-		<Item
-			isAction
-			onClick={ () => navigator.push( path, { isBack } ) }
-			{ ...props }
-		>
+		<Item onClick={ () => navigator.push( path, { isBack } ) } { ...props }>
 			<HStack justify="flex-start">
 				{ icon && (
 					<FlexItem>