diff --git a/cmd/antrea-agent/options.go b/cmd/antrea-agent/options.go
index 732a6abfb6c..faaa1934428 100644
--- a/cmd/antrea-agent/options.go
+++ b/cmd/antrea-agent/options.go
@@ -734,8 +734,8 @@ func (o *Options) validateSecondaryNetworkConfig() error {
 	if brConfig.BridgeName == "" {
 		return fmt.Errorf("bridge name is not provided for the secondary network OVS bridge")
 	}
-	if len(brConfig.PhysicalInterfaces) > 1 {
-		return fmt.Errorf("at most one physical interface can be specified for the secondary network OVS bridge")
+	if len(brConfig.PhysicalInterfaces) > 8 {
+		return fmt.Errorf("at most eight physical interfaces can be specified for the secondary network OVS bridge")
 	}
 
 	return nil
diff --git a/cmd/antrea-agent/options_test.go b/cmd/antrea-agent/options_test.go
index 22c612d7c5f..4b62f775800 100644
--- a/cmd/antrea-agent/options_test.go
+++ b/cmd/antrea-agent/options_test.go
@@ -319,8 +319,8 @@ func TestOptionsValidateSecondaryNetworkConfig(t *testing.T) {
 			name:               "two interfaces",
 			featureGateValue:   true,
 			ovsBridges:         []string{"br1"},
-			physicalInterfaces: []string{"eth1", "eth2"},
-			expectedErr:        "at most one physical interface can be specified for the secondary network OVS bridge",
+			physicalInterfaces: []string{"eth1", "eth2", "eth3", "eth4", "eth5", "eth6", "eth7", "eth8", "eth9"},
+			expectedErr:        "at most eight physical interfaces can be specified for the secondary network OVS bridge",
 		},
 	}
 	for _, tc := range tests {
diff --git a/docs/secondary-network.md b/docs/secondary-network.md
index 8b8d122d198..31f7b24dd92 100644
--- a/docs/secondary-network.md
+++ b/docs/secondary-network.md
@@ -68,8 +68,8 @@ data:
 ```
 
 At the moment, Antrea supports only a single OVS bridge for secondary networks,
-and supports only a single physical interface on the bridge. The physical
-interface cannot be the Node's management interface, otherwise the Node's
+and supports upto eight physical interfaces on the bridge. The physical
+interfaces cannot be the Node's management interface, otherwise the Node's
 management network connectivity can be broken after `antrea-agent` creates the
 OVS bridge and moves the management interface to the bridge.
 
diff --git a/pkg/agent/secondarynetwork/init.go b/pkg/agent/secondarynetwork/init.go
index abc3351c001..7333b6e5878 100644
--- a/pkg/agent/secondarynetwork/init.go
+++ b/pkg/agent/secondarynetwork/init.go
@@ -34,7 +34,7 @@ import (
 )
 
 var (
-	// Funcs which will be orridden with mock funcs in tests.
+	// Funcs which will be overridden with mock funcs in tests.
 	interfaceByNameFn = net.InterfaceByName
 	newOVSBridgeFn    = ovsconfig.NewOVSBridge
 )
@@ -82,9 +82,7 @@ func createOVSBridge(bridges []agentconfig.OVSBridgeConfig, ovsdb *ovsdb.OVSDB)
 	// Only one OVS bridge is supported.
 	bridgeConfig := bridges[0]
 
-	phyInterface := ""
-	if len(bridgeConfig.PhysicalInterfaces) > 0 {
-		phyInterface = bridgeConfig.PhysicalInterfaces[0]
+	for _, phyInterface := range bridgeConfig.PhysicalInterfaces {
 		if _, err := interfaceByNameFn(phyInterface); err != nil {
 			return nil, fmt.Errorf("failed to get interface %s: %v", phyInterface, err)
 		}
@@ -96,21 +94,17 @@ func createOVSBridge(bridges []agentconfig.OVSBridgeConfig, ovsdb *ovsdb.OVSDB)
 	}
 	klog.InfoS("OVS bridge created", "bridge", bridgeConfig.BridgeName)
 
-	if phyInterface == "" {
-		return ovsBridgeClient, nil
-	}
-
-	if _, err := ovsBridgeClient.GetOFPort(phyInterface, false); err == nil {
-		klog.V(2).InfoS("Physical interface already connected to OVS bridge, skip the configuration", "device", phyInterface, "bridge", bridgeConfig.BridgeName)
-		return ovsBridgeClient, nil
-	}
+	for i, phyInterface := range bridgeConfig.PhysicalInterfaces {
+		if _, err := ovsBridgeClient.GetOFPort(phyInterface, false); err == nil {
+			klog.V(2).InfoS("Physical interface already connected to OVS bridge, skip the configuration", "device", phyInterface, "bridge", bridgeConfig.BridgeName)
+			continue
+		}
 
-	_, err := ovsBridgeClient.CreateUplinkPort(phyInterface, 0, map[string]interface{}{interfacestore.AntreaInterfaceTypeKey: interfacestore.AntreaUplink})
-	if err != nil {
-		return nil, fmt.Errorf("failed to create OVS uplink port %s: %v", phyInterface, err)
+		if _, err := ovsBridgeClient.CreateUplinkPort(phyInterface, int32(i), map[string]interface{}{interfacestore.AntreaInterfaceTypeKey: interfacestore.AntreaUplink}); err != nil {
+			return nil, fmt.Errorf("failed to create OVS uplink port %s: %v", phyInterface, err)
+		}
+		klog.InfoS("Physical interface added to OVS bridge", "device", phyInterface, "bridge", bridgeConfig.BridgeName)
 	}
-	klog.InfoS("Physical interface added to OVS bridge", "device", phyInterface, "bridge", bridgeConfig.BridgeName)
-
 	return ovsBridgeClient, nil
 }
 
diff --git a/pkg/agent/secondarynetwork/init_test.go b/pkg/agent/secondarynetwork/init_test.go
index 56845365e94..b9be6fe0fd2 100644
--- a/pkg/agent/secondarynetwork/init_test.go
+++ b/pkg/agent/secondarynetwork/init_test.go
@@ -82,6 +82,8 @@ func TestCreateOVSBridge(t *testing.T) {
 				m.EXPECT().Create().Return(nil)
 				m.EXPECT().GetOFPort("eth1", false).Return(int32(0), ovsconfig.InvalidArgumentsError("port not found"))
 				m.EXPECT().CreateUplinkPort("eth1", int32(0), map[string]interface{}{"antrea-type": "uplink"}).Return("", nil)
+				m.EXPECT().GetOFPort("eth2", false).Return(int32(1), ovsconfig.InvalidArgumentsError("port not found"))
+				m.EXPECT().CreateUplinkPort("eth2", int32(1), map[string]interface{}{"antrea-type": "uplink"}).Return("", nil)
 			},
 		},
 		{
diff --git a/pkg/config/agent/config.go b/pkg/config/agent/config.go
index 21d3ac50cdf..1c59f48b3f9 100644
--- a/pkg/config/agent/config.go
+++ b/pkg/config/agent/config.go
@@ -398,7 +398,6 @@ type SecondaryNetworkConfig struct {
 
 type OVSBridgeConfig struct {
 	BridgeName string `yaml:"bridgeName"`
-	// Names of physical interfaces to be connected to the bridge. At the moment,
-	// only a single physical interface is supported.
+	// Names of physical interfaces to be connected to the bridge.
 	PhysicalInterfaces []string `yaml:"physicalInterfaces,omitempty"`
 }