diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/IntegTestDefaultTestDeployAssertE3E7D2A4.assets.json b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/IntegTestDefaultTestDeployAssertE3E7D2A4.assets.json
index 1e34ca625f5ac..d48f521ed445d 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/IntegTestDefaultTestDeployAssertE3E7D2A4.assets.json
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/IntegTestDefaultTestDeployAssertE3E7D2A4.assets.json
@@ -1,5 +1,5 @@
 {
-  "version": "32.0.0",
+  "version": "33.0.0",
   "files": {
     "21fbb51d7b23f6a6c262b46a9caee79d744a3ac019fd45422d988b96d44b2a22": {
       "source": {
diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack1.assets.json b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack1.assets.json
index c02b32ab9a840..f245f57c4b8fc 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack1.assets.json
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack1.assets.json
@@ -1,7 +1,7 @@
 {
-  "version": "32.0.0",
+  "version": "33.0.0",
   "files": {
-    "1e011d502b5efbd34b58f00a70ccf34fb812c6af28ee32ffe796b007503169f1": {
+    "0a057723ee23dd83fb3c1e47ef1ceee53b1aeb809908b5e428c6081b212a6ada": {
       "source": {
         "path": "Stack1.template.json",
         "packaging": "file"
@@ -9,7 +9,7 @@
       "destinations": {
         "current_account-current_region": {
           "bucketName": "cdk-hnb659fds-assets-${AWS::AccountId}-${AWS::Region}",
-          "objectKey": "1e011d502b5efbd34b58f00a70ccf34fb812c6af28ee32ffe796b007503169f1.json",
+          "objectKey": "0a057723ee23dd83fb3c1e47ef1ceee53b1aeb809908b5e428c6081b212a6ada.json",
           "assumeRoleArn": "arn:${AWS::Partition}:iam::${AWS::AccountId}:role/cdk-hnb659fds-file-publishing-role-${AWS::AccountId}-${AWS::Region}"
         }
       }
diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack1.template.json b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack1.template.json
index e4cdf818a89a7..1ab0e04b8a60f 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack1.template.json
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack1.template.json
@@ -58,18 +58,12 @@
   "MyFunc11BE70A62": {
    "Type": "AWS::Lambda::Function",
    "Properties": {
-    "Code": {
-     "ZipFile": "exports.handler = function handler(event, _context, callback) {\n    console.log(JSON.stringify(event, undefined, 2));\n    return callback();\n}"
-    },
-    "Role": {
-     "Fn::GetAtt": [
-      "MyFunc1ServiceRoleF96C5B5C",
-      "Arn"
-     ]
-    },
     "Architectures": [
      "arm64"
     ],
+    "Code": {
+     "ZipFile": "exports.handler = function handler(event, _context, callback) {\n    console.log(JSON.stringify(event, undefined, 2));\n    return callback();\n}"
+    },
     "Environment": {
      "Variables": {
       "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-handler"
@@ -78,15 +72,24 @@
     "Handler": "index.handler",
     "Layers": [
      {
-      "Fn::FindInMap": [
-       "AdotlambdalayerMap",
-       {
-        "Ref": "AWS::Region"
-       },
-       "JAVASCRIPTxSDKx1x7x0xarm64"
+      "Fn::Join": [
+       "",
+       [
+        "arn:aws:lambda:",
+        {
+         "Ref": "AWS::Region"
+        },
+        ":901920570463:layer:aws-otel-nodejs-arm64-ver-1-15-0:2"
+       ]
       ]
      }
     ],
+    "Role": {
+     "Fn::GetAtt": [
+      "MyFunc1ServiceRoleF96C5B5C",
+      "Arn"
+     ]
+    },
     "Runtime": "nodejs14.x",
     "TracingConfig": {
      "Mode": "Active"
@@ -158,12 +161,6 @@
     "Code": {
      "ZipFile": "def handler(event, context): pass"
     },
-    "Role": {
-     "Fn::GetAtt": [
-      "MyFunc2ServiceRole68E50443",
-      "Arn"
-     ]
-    },
     "Environment": {
      "Variables": {
       "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-instrument"
@@ -179,11 +176,17 @@
         {
          "Ref": "AWS::Region"
         },
-        ":901920570463:layer:aws-otel-python-amd64-ver-1-18-0:2"
+        ":901920570463:layer:aws-otel-python-amd64-ver-1-19-0:2"
        ]
       ]
      }
     ],
+    "Role": {
+     "Fn::GetAtt": [
+      "MyFunc2ServiceRole68E50443",
+      "Arn"
+     ]
+    },
     "Runtime": "python3.9",
     "TracingConfig": {
      "Mode": "Active"
@@ -255,12 +258,6 @@
     "Code": {
      "ZipFile": "def handler(event, context): pass"
     },
-    "Role": {
-     "Fn::GetAtt": [
-      "MyFunc3ServiceRoleA69795ED",
-      "Arn"
-     ]
-    },
     "Environment": {
      "Variables": {
       "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-instrument"
@@ -276,11 +273,17 @@
         {
          "Ref": "AWS::Region"
         },
-        ":901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-19-0:1"
+        ":901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-28-1:1"
        ]
       ]
      }
     ],
+    "Role": {
+     "Fn::GetAtt": [
+      "MyFunc3ServiceRoleA69795ED",
+      "Arn"
+     ]
+    },
     "Runtime": "python3.9",
     "TracingConfig": {
      "Mode": "Active"
@@ -352,12 +355,6 @@
     "Code": {
      "ZipFile": "def handler(event, context): pass"
     },
-    "Role": {
-     "Fn::GetAtt": [
-      "MyFunc4ServiceRole93C4DEFF",
-      "Arn"
-     ]
-    },
     "Environment": {
      "Variables": {
       "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-instrument"
@@ -373,11 +370,17 @@
         {
          "Ref": "AWS::Region"
         },
-        ":901920570463:layer:aws-otel-java-agent-amd64-ver-1-19-2:1"
+        ":901920570463:layer:aws-otel-java-agent-amd64-ver-1-28-1:1"
        ]
       ]
      }
     ],
+    "Role": {
+     "Fn::GetAtt": [
+      "MyFunc4ServiceRole93C4DEFF",
+      "Arn"
+     ]
+    },
     "Runtime": "python3.9",
     "TracingConfig": {
      "Mode": "Active"
@@ -449,12 +452,6 @@
     "Code": {
      "ZipFile": "def handler(event, context): pass"
     },
-    "Role": {
-     "Fn::GetAtt": [
-      "MyFunc5ServiceRoleFE4CE92B",
-      "Arn"
-     ]
-    },
     "Environment": {
      "Variables": {
       "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-instrument"
@@ -470,11 +467,17 @@
         {
          "Ref": "AWS::Region"
         },
-        ":901920570463:layer:aws-otel-collector-amd64-ver-0-62-1:1"
+        ":901920570463:layer:aws-otel-collector-amd64-ver-0-82-0:1"
        ]
       ]
      }
     ],
+    "Role": {
+     "Fn::GetAtt": [
+      "MyFunc5ServiceRoleFE4CE92B",
+      "Arn"
+     ]
+    },
     "Runtime": "python3.9",
     "TracingConfig": {
      "Mode": "Active"
@@ -486,58 +489,6 @@
    ]
   }
  },
- "Mappings": {
-  "AdotlambdalayerMap": {
-   "ap-northeast-1": {
-    "JAVASCRIPTxSDKx1x7x0xarm64": "arn:aws:lambda:ap-northeast-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-7-0:2"
-   },
-   "ap-northeast-2": {
-    "JAVASCRIPTxSDKx1x7x0xarm64": "arn:aws:lambda:ap-northeast-2:901920570463:layer:aws-otel-nodejs-arm64-ver-1-7-0:1"
-   },
-   "ap-south-1": {
-    "JAVASCRIPTxSDKx1x7x0xarm64": "arn:aws:lambda:ap-south-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-7-0:2"
-   },
-   "ap-southeast-1": {
-    "JAVASCRIPTxSDKx1x7x0xarm64": "arn:aws:lambda:ap-southeast-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-7-0:2"
-   },
-   "ap-southeast-2": {
-    "JAVASCRIPTxSDKx1x7x0xarm64": "arn:aws:lambda:ap-southeast-2:901920570463:layer:aws-otel-nodejs-arm64-ver-1-7-0:2"
-   },
-   "ca-central-1": {
-    "JAVASCRIPTxSDKx1x7x0xarm64": "arn:aws:lambda:ca-central-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-7-0:1"
-   },
-   "eu-central-1": {
-    "JAVASCRIPTxSDKx1x7x0xarm64": "arn:aws:lambda:eu-central-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-7-0:2"
-   },
-   "eu-north-1": {
-    "JAVASCRIPTxSDKx1x7x0xarm64": "arn:aws:lambda:eu-north-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-7-0:1"
-   },
-   "eu-west-1": {
-    "JAVASCRIPTxSDKx1x7x0xarm64": "arn:aws:lambda:eu-west-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-7-0:2"
-   },
-   "eu-west-2": {
-    "JAVASCRIPTxSDKx1x7x0xarm64": "arn:aws:lambda:eu-west-2:901920570463:layer:aws-otel-nodejs-arm64-ver-1-7-0:2"
-   },
-   "eu-west-3": {
-    "JAVASCRIPTxSDKx1x7x0xarm64": "arn:aws:lambda:eu-west-3:901920570463:layer:aws-otel-nodejs-arm64-ver-1-7-0:1"
-   },
-   "sa-east-1": {
-    "JAVASCRIPTxSDKx1x7x0xarm64": "arn:aws:lambda:sa-east-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-7-0:1"
-   },
-   "us-east-1": {
-    "JAVASCRIPTxSDKx1x7x0xarm64": "arn:aws:lambda:us-east-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-7-0:2"
-   },
-   "us-east-2": {
-    "JAVASCRIPTxSDKx1x7x0xarm64": "arn:aws:lambda:us-east-2:901920570463:layer:aws-otel-nodejs-arm64-ver-1-7-0:2"
-   },
-   "us-west-1": {
-    "JAVASCRIPTxSDKx1x7x0xarm64": "arn:aws:lambda:us-west-1:901920570463:layer:aws-otel-nodejs-arm64-ver-1-7-0:1"
-   },
-   "us-west-2": {
-    "JAVASCRIPTxSDKx1x7x0xarm64": "arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-nodejs-arm64-ver-1-7-0:2"
-   }
-  }
- },
  "Parameters": {
   "BootstrapVersion": {
    "Type": "AWS::SSM::Parameter::Value<String>",
diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack2.assets.json b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack2.assets.json
index 359267ab7abf7..15522d1f2a230 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack2.assets.json
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack2.assets.json
@@ -1,7 +1,7 @@
 {
-  "version": "32.0.0",
+  "version": "33.0.0",
   "files": {
-    "45de98e0bad004888e2b3a349cf7b69b966cff9ba6effb99b03e462c69adae21": {
+    "5f65e129af8492d163c28dcb80d294d6b4b93120afa30f095a40a2a5ea5f6296": {
       "source": {
         "path": "Stack2.template.json",
         "packaging": "file"
@@ -9,7 +9,7 @@
       "destinations": {
         "current_account-current_region": {
           "bucketName": "cdk-hnb659fds-assets-${AWS::AccountId}-${AWS::Region}",
-          "objectKey": "45de98e0bad004888e2b3a349cf7b69b966cff9ba6effb99b03e462c69adae21.json",
+          "objectKey": "5f65e129af8492d163c28dcb80d294d6b4b93120afa30f095a40a2a5ea5f6296.json",
           "assumeRoleArn": "arn:${AWS::Partition}:iam::${AWS::AccountId}:role/cdk-hnb659fds-file-publishing-role-${AWS::AccountId}-${AWS::Region}"
         }
       }
diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack2.template.json b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack2.template.json
index a2f2184183646..9a9a84e013628 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack2.template.json
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/Stack2.template.json
@@ -58,18 +58,12 @@
   "MyFunc11BE70A62": {
    "Type": "AWS::Lambda::Function",
    "Properties": {
-    "Code": {
-     "ZipFile": "exports.handler = function handler(event, _context, callback) {\n    console.log(JSON.stringify(event, undefined, 2));\n    return callback();\n}"
-    },
-    "Role": {
-     "Fn::GetAtt": [
-      "MyFunc1ServiceRoleF96C5B5C",
-      "Arn"
-     ]
-    },
     "Architectures": [
      "x86_64"
     ],
+    "Code": {
+     "ZipFile": "exports.handler = function handler(event, _context, callback) {\n    console.log(JSON.stringify(event, undefined, 2));\n    return callback();\n}"
+    },
     "Environment": {
      "Variables": {
       "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-handler"
@@ -85,11 +79,17 @@
         {
          "Ref": "AWS::Region"
         },
-        ":901920570463:layer:aws-otel-nodejs-amd64-ver-1-7-0:2"
+        ":901920570463:layer:aws-otel-nodejs-amd64-ver-1-15-0:2"
        ]
       ]
      }
     ],
+    "Role": {
+     "Fn::GetAtt": [
+      "MyFunc1ServiceRoleF96C5B5C",
+      "Arn"
+     ]
+    },
     "Runtime": "nodejs14.x",
     "TracingConfig": {
      "Mode": "Active"
@@ -161,12 +161,6 @@
     "Code": {
      "ZipFile": "def handler(event, context): pass"
     },
-    "Role": {
-     "Fn::GetAtt": [
-      "MyFunc2ServiceRole68E50443",
-      "Arn"
-     ]
-    },
     "Environment": {
      "Variables": {
       "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-instrument"
@@ -182,11 +176,17 @@
         {
          "Ref": "AWS::Region"
         },
-        ":901920570463:layer:aws-otel-python-amd64-ver-1-18-0:2"
+        ":901920570463:layer:aws-otel-python-amd64-ver-1-19-0:2"
        ]
       ]
      }
     ],
+    "Role": {
+     "Fn::GetAtt": [
+      "MyFunc2ServiceRole68E50443",
+      "Arn"
+     ]
+    },
     "Runtime": "python3.9",
     "TracingConfig": {
      "Mode": "Active"
@@ -258,12 +258,6 @@
     "Code": {
      "ZipFile": "def handler(event, context): pass"
     },
-    "Role": {
-     "Fn::GetAtt": [
-      "MyFunc3ServiceRoleA69795ED",
-      "Arn"
-     ]
-    },
     "Environment": {
      "Variables": {
       "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-instrument"
@@ -279,11 +273,17 @@
         {
          "Ref": "AWS::Region"
         },
-        ":901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-19-0:1"
+        ":901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-28-1:1"
        ]
       ]
      }
     ],
+    "Role": {
+     "Fn::GetAtt": [
+      "MyFunc3ServiceRoleA69795ED",
+      "Arn"
+     ]
+    },
     "Runtime": "python3.9",
     "TracingConfig": {
      "Mode": "Active"
@@ -355,12 +355,6 @@
     "Code": {
      "ZipFile": "def handler(event, context): pass"
     },
-    "Role": {
-     "Fn::GetAtt": [
-      "MyFunc4ServiceRole93C4DEFF",
-      "Arn"
-     ]
-    },
     "Environment": {
      "Variables": {
       "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-instrument"
@@ -376,11 +370,17 @@
         {
          "Ref": "AWS::Region"
         },
-        ":901920570463:layer:aws-otel-java-agent-amd64-ver-1-19-2:1"
+        ":901920570463:layer:aws-otel-java-agent-amd64-ver-1-28-1:1"
        ]
       ]
      }
     ],
+    "Role": {
+     "Fn::GetAtt": [
+      "MyFunc4ServiceRole93C4DEFF",
+      "Arn"
+     ]
+    },
     "Runtime": "python3.9",
     "TracingConfig": {
      "Mode": "Active"
@@ -452,12 +452,6 @@
     "Code": {
      "ZipFile": "def handler(event, context): pass"
     },
-    "Role": {
-     "Fn::GetAtt": [
-      "MyFunc5ServiceRoleFE4CE92B",
-      "Arn"
-     ]
-    },
     "Environment": {
      "Variables": {
       "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-instrument"
@@ -473,11 +467,17 @@
         {
          "Ref": "AWS::Region"
         },
-        ":901920570463:layer:aws-otel-collector-amd64-ver-0-62-1:1"
+        ":901920570463:layer:aws-otel-collector-amd64-ver-0-82-0:1"
        ]
       ]
      }
     ],
+    "Role": {
+     "Fn::GetAtt": [
+      "MyFunc5ServiceRoleFE4CE92B",
+      "Arn"
+     ]
+    },
     "Runtime": "python3.9",
     "TracingConfig": {
      "Mode": "Active"
diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/cdk.out b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/cdk.out
index f0b901e7c06e5..560dae10d018f 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/cdk.out
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/cdk.out
@@ -1 +1 @@
-{"version":"32.0.0"}
\ No newline at end of file
+{"version":"33.0.0"}
\ No newline at end of file
diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/integ.json b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/integ.json
index 263e489ed7e1c..e78aad4885bfa 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/integ.json
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/integ.json
@@ -1,5 +1,5 @@
 {
-  "version": "32.0.0",
+  "version": "33.0.0",
   "testCases": {
     "IntegTest/DefaultTest": {
       "stacks": [
diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/manifest.json b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/manifest.json
index 1de152555a3ca..0c44d10e95412 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/manifest.json
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/manifest.json
@@ -1,5 +1,5 @@
 {
-  "version": "32.0.0",
+  "version": "33.0.0",
   "artifacts": {
     "Stack1.assets": {
       "type": "cdk:asset-manifest",
@@ -17,7 +17,7 @@
         "validateOnSynth": false,
         "assumeRoleArn": "arn:${AWS::Partition}:iam::${AWS::AccountId}:role/cdk-hnb659fds-deploy-role-${AWS::AccountId}-${AWS::Region}",
         "cloudFormationExecutionRoleArn": "arn:${AWS::Partition}:iam::${AWS::AccountId}:role/cdk-hnb659fds-cfn-exec-role-${AWS::AccountId}-${AWS::Region}",
-        "stackTemplateAssetObjectUrl": "s3://cdk-hnb659fds-assets-${AWS::AccountId}-${AWS::Region}/1e011d502b5efbd34b58f00a70ccf34fb812c6af28ee32ffe796b007503169f1.json",
+        "stackTemplateAssetObjectUrl": "s3://cdk-hnb659fds-assets-${AWS::AccountId}-${AWS::Region}/0a057723ee23dd83fb3c1e47ef1ceee53b1aeb809908b5e428c6081b212a6ada.json",
         "requiresBootstrapStackVersion": 6,
         "bootstrapStackVersionSsmParameter": "/cdk-bootstrap/hnb659fds/version",
         "additionalDependencies": [
@@ -51,12 +51,6 @@
             "data": "MyFunc11BE70A62"
           }
         ],
-        "/Stack1/Adot-lambda-layerMap": [
-          {
-            "type": "aws:cdk:logicalId",
-            "data": "AdotlambdalayerMap"
-          }
-        ],
         "/Stack1/MyFunc2/ServiceRole/Resource": [
           {
             "type": "aws:cdk:logicalId",
@@ -160,7 +154,7 @@
         "validateOnSynth": false,
         "assumeRoleArn": "arn:${AWS::Partition}:iam::${AWS::AccountId}:role/cdk-hnb659fds-deploy-role-${AWS::AccountId}-${AWS::Region}",
         "cloudFormationExecutionRoleArn": "arn:${AWS::Partition}:iam::${AWS::AccountId}:role/cdk-hnb659fds-cfn-exec-role-${AWS::AccountId}-${AWS::Region}",
-        "stackTemplateAssetObjectUrl": "s3://cdk-hnb659fds-assets-${AWS::AccountId}-${AWS::Region}/45de98e0bad004888e2b3a349cf7b69b966cff9ba6effb99b03e462c69adae21.json",
+        "stackTemplateAssetObjectUrl": "s3://cdk-hnb659fds-assets-${AWS::AccountId}-${AWS::Region}/5f65e129af8492d163c28dcb80d294d6b4b93120afa30f095a40a2a5ea5f6296.json",
         "requiresBootstrapStackVersion": 6,
         "bootstrapStackVersionSsmParameter": "/cdk-bootstrap/hnb659fds/version",
         "additionalDependencies": [
diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/tree.json b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/tree.json
index 1aace82f92764..833ab4629374f 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/tree.json
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.js.snapshot/tree.json
@@ -117,18 +117,12 @@
                 "attributes": {
                   "aws:cdk:cloudformation:type": "AWS::Lambda::Function",
                   "aws:cdk:cloudformation:props": {
-                    "code": {
-                      "zipFile": "exports.handler = function handler(event, _context, callback) {\n    console.log(JSON.stringify(event, undefined, 2));\n    return callback();\n}"
-                    },
-                    "role": {
-                      "Fn::GetAtt": [
-                        "MyFunc1ServiceRoleF96C5B5C",
-                        "Arn"
-                      ]
-                    },
                     "architectures": [
                       "arm64"
                     ],
+                    "code": {
+                      "zipFile": "exports.handler = function handler(event, _context, callback) {\n    console.log(JSON.stringify(event, undefined, 2));\n    return callback();\n}"
+                    },
                     "environment": {
                       "variables": {
                         "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-handler"
@@ -137,15 +131,24 @@
                     "handler": "index.handler",
                     "layers": [
                       {
-                        "Fn::FindInMap": [
-                          "AdotlambdalayerMap",
-                          {
-                            "Ref": "AWS::Region"
-                          },
-                          "JAVASCRIPTxSDKx1x7x0xarm64"
+                        "Fn::Join": [
+                          "",
+                          [
+                            "arn:aws:lambda:",
+                            {
+                              "Ref": "AWS::Region"
+                            },
+                            ":901920570463:layer:aws-otel-nodejs-arm64-ver-1-15-0:2"
+                          ]
                         ]
                       }
                     ],
+                    "role": {
+                      "Fn::GetAtt": [
+                        "MyFunc1ServiceRoleF96C5B5C",
+                        "Arn"
+                      ]
+                    },
                     "runtime": "nodejs14.x",
                     "tracingConfig": {
                       "mode": "Active"
@@ -171,14 +174,6 @@
               "version": "0.0.0"
             }
           },
-          "Adot-lambda-layerMap": {
-            "id": "Adot-lambda-layerMap",
-            "path": "Stack1/Adot-lambda-layerMap",
-            "constructInfo": {
-              "fqn": "aws-cdk-lib.CfnMapping",
-              "version": "0.0.0"
-            }
-          },
           "MyFunc2": {
             "id": "MyFunc2",
             "path": "Stack1/MyFunc2",
@@ -291,12 +286,6 @@
                     "code": {
                       "zipFile": "def handler(event, context): pass"
                     },
-                    "role": {
-                      "Fn::GetAtt": [
-                        "MyFunc2ServiceRole68E50443",
-                        "Arn"
-                      ]
-                    },
                     "environment": {
                       "variables": {
                         "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-instrument"
@@ -312,11 +301,17 @@
                             {
                               "Ref": "AWS::Region"
                             },
-                            ":901920570463:layer:aws-otel-python-amd64-ver-1-18-0:2"
+                            ":901920570463:layer:aws-otel-python-amd64-ver-1-19-0:2"
                           ]
                         ]
                       }
                     ],
+                    "role": {
+                      "Fn::GetAtt": [
+                        "MyFunc2ServiceRole68E50443",
+                        "Arn"
+                      ]
+                    },
                     "runtime": "python3.9",
                     "tracingConfig": {
                       "mode": "Active"
@@ -454,12 +449,6 @@
                     "code": {
                       "zipFile": "def handler(event, context): pass"
                     },
-                    "role": {
-                      "Fn::GetAtt": [
-                        "MyFunc3ServiceRoleA69795ED",
-                        "Arn"
-                      ]
-                    },
                     "environment": {
                       "variables": {
                         "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-instrument"
@@ -475,11 +464,17 @@
                             {
                               "Ref": "AWS::Region"
                             },
-                            ":901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-19-0:1"
+                            ":901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-28-1:1"
                           ]
                         ]
                       }
                     ],
+                    "role": {
+                      "Fn::GetAtt": [
+                        "MyFunc3ServiceRoleA69795ED",
+                        "Arn"
+                      ]
+                    },
                     "runtime": "python3.9",
                     "tracingConfig": {
                       "mode": "Active"
@@ -617,12 +612,6 @@
                     "code": {
                       "zipFile": "def handler(event, context): pass"
                     },
-                    "role": {
-                      "Fn::GetAtt": [
-                        "MyFunc4ServiceRole93C4DEFF",
-                        "Arn"
-                      ]
-                    },
                     "environment": {
                       "variables": {
                         "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-instrument"
@@ -638,11 +627,17 @@
                             {
                               "Ref": "AWS::Region"
                             },
-                            ":901920570463:layer:aws-otel-java-agent-amd64-ver-1-19-2:1"
+                            ":901920570463:layer:aws-otel-java-agent-amd64-ver-1-28-1:1"
                           ]
                         ]
                       }
                     ],
+                    "role": {
+                      "Fn::GetAtt": [
+                        "MyFunc4ServiceRole93C4DEFF",
+                        "Arn"
+                      ]
+                    },
                     "runtime": "python3.9",
                     "tracingConfig": {
                       "mode": "Active"
@@ -780,12 +775,6 @@
                     "code": {
                       "zipFile": "def handler(event, context): pass"
                     },
-                    "role": {
-                      "Fn::GetAtt": [
-                        "MyFunc5ServiceRoleFE4CE92B",
-                        "Arn"
-                      ]
-                    },
                     "environment": {
                       "variables": {
                         "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-instrument"
@@ -801,11 +790,17 @@
                             {
                               "Ref": "AWS::Region"
                             },
-                            ":901920570463:layer:aws-otel-collector-amd64-ver-0-62-1:1"
+                            ":901920570463:layer:aws-otel-collector-amd64-ver-0-82-0:1"
                           ]
                         ]
                       }
                     ],
+                    "role": {
+                      "Fn::GetAtt": [
+                        "MyFunc5ServiceRoleFE4CE92B",
+                        "Arn"
+                      ]
+                    },
                     "runtime": "python3.9",
                     "tracingConfig": {
                       "mode": "Active"
@@ -966,18 +961,12 @@
                 "attributes": {
                   "aws:cdk:cloudformation:type": "AWS::Lambda::Function",
                   "aws:cdk:cloudformation:props": {
-                    "code": {
-                      "zipFile": "exports.handler = function handler(event, _context, callback) {\n    console.log(JSON.stringify(event, undefined, 2));\n    return callback();\n}"
-                    },
-                    "role": {
-                      "Fn::GetAtt": [
-                        "MyFunc1ServiceRoleF96C5B5C",
-                        "Arn"
-                      ]
-                    },
                     "architectures": [
                       "x86_64"
                     ],
+                    "code": {
+                      "zipFile": "exports.handler = function handler(event, _context, callback) {\n    console.log(JSON.stringify(event, undefined, 2));\n    return callback();\n}"
+                    },
                     "environment": {
                       "variables": {
                         "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-handler"
@@ -993,11 +982,17 @@
                             {
                               "Ref": "AWS::Region"
                             },
-                            ":901920570463:layer:aws-otel-nodejs-amd64-ver-1-7-0:2"
+                            ":901920570463:layer:aws-otel-nodejs-amd64-ver-1-15-0:2"
                           ]
                         ]
                       }
                     ],
+                    "role": {
+                      "Fn::GetAtt": [
+                        "MyFunc1ServiceRoleF96C5B5C",
+                        "Arn"
+                      ]
+                    },
                     "runtime": "nodejs14.x",
                     "tracingConfig": {
                       "mode": "Active"
@@ -1135,12 +1130,6 @@
                     "code": {
                       "zipFile": "def handler(event, context): pass"
                     },
-                    "role": {
-                      "Fn::GetAtt": [
-                        "MyFunc2ServiceRole68E50443",
-                        "Arn"
-                      ]
-                    },
                     "environment": {
                       "variables": {
                         "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-instrument"
@@ -1156,11 +1145,17 @@
                             {
                               "Ref": "AWS::Region"
                             },
-                            ":901920570463:layer:aws-otel-python-amd64-ver-1-18-0:2"
+                            ":901920570463:layer:aws-otel-python-amd64-ver-1-19-0:2"
                           ]
                         ]
                       }
                     ],
+                    "role": {
+                      "Fn::GetAtt": [
+                        "MyFunc2ServiceRole68E50443",
+                        "Arn"
+                      ]
+                    },
                     "runtime": "python3.9",
                     "tracingConfig": {
                       "mode": "Active"
@@ -1298,12 +1293,6 @@
                     "code": {
                       "zipFile": "def handler(event, context): pass"
                     },
-                    "role": {
-                      "Fn::GetAtt": [
-                        "MyFunc3ServiceRoleA69795ED",
-                        "Arn"
-                      ]
-                    },
                     "environment": {
                       "variables": {
                         "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-instrument"
@@ -1319,11 +1308,17 @@
                             {
                               "Ref": "AWS::Region"
                             },
-                            ":901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-19-0:1"
+                            ":901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-28-1:1"
                           ]
                         ]
                       }
                     ],
+                    "role": {
+                      "Fn::GetAtt": [
+                        "MyFunc3ServiceRoleA69795ED",
+                        "Arn"
+                      ]
+                    },
                     "runtime": "python3.9",
                     "tracingConfig": {
                       "mode": "Active"
@@ -1461,12 +1456,6 @@
                     "code": {
                       "zipFile": "def handler(event, context): pass"
                     },
-                    "role": {
-                      "Fn::GetAtt": [
-                        "MyFunc4ServiceRole93C4DEFF",
-                        "Arn"
-                      ]
-                    },
                     "environment": {
                       "variables": {
                         "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-instrument"
@@ -1482,11 +1471,17 @@
                             {
                               "Ref": "AWS::Region"
                             },
-                            ":901920570463:layer:aws-otel-java-agent-amd64-ver-1-19-2:1"
+                            ":901920570463:layer:aws-otel-java-agent-amd64-ver-1-28-1:1"
                           ]
                         ]
                       }
                     ],
+                    "role": {
+                      "Fn::GetAtt": [
+                        "MyFunc4ServiceRole93C4DEFF",
+                        "Arn"
+                      ]
+                    },
                     "runtime": "python3.9",
                     "tracingConfig": {
                       "mode": "Active"
@@ -1624,12 +1619,6 @@
                     "code": {
                       "zipFile": "def handler(event, context): pass"
                     },
-                    "role": {
-                      "Fn::GetAtt": [
-                        "MyFunc5ServiceRoleFE4CE92B",
-                        "Arn"
-                      ]
-                    },
                     "environment": {
                       "variables": {
                         "AWS_LAMBDA_EXEC_WRAPPER": "/opt/otel-instrument"
@@ -1645,11 +1634,17 @@
                             {
                               "Ref": "AWS::Region"
                             },
-                            ":901920570463:layer:aws-otel-collector-amd64-ver-0-62-1:1"
+                            ":901920570463:layer:aws-otel-collector-amd64-ver-0-82-0:1"
                           ]
                         ]
                       }
                     ],
+                    "role": {
+                      "Fn::GetAtt": [
+                        "MyFunc5ServiceRoleFE4CE92B",
+                        "Arn"
+                      ]
+                    },
                     "runtime": "python3.9",
                     "tracingConfig": {
                       "mode": "Active"
@@ -1710,7 +1705,7 @@
                 "path": "IntegTest/DefaultTest/Default",
                 "constructInfo": {
                   "fqn": "constructs.Construct",
-                  "version": "10.2.55"
+                  "version": "10.2.69"
                 }
               },
               "DeployAssert": {
@@ -1756,7 +1751,7 @@
         "path": "Tree",
         "constructInfo": {
           "fqn": "constructs.Construct",
-          "version": "10.2.55"
+          "version": "10.2.69"
         }
       }
     },
diff --git a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.ts b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.ts
index 07c6e6f1e74f2..717ffb123f421 100644
--- a/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.ts
+++ b/packages/@aws-cdk-testing/framework-integ/test/aws-lambda/test/integ.lambda-adot.ts
@@ -32,7 +32,7 @@ class StackUnderTest extends Stack {
       code: Code.fromInline(`exports.handler = ${handler.toString()}`),
       architecture: props.architecture,
       adotInstrumentation: {
-        layerVersion: AdotLayerVersion.fromJavaScriptSdkLayerVersion(AdotLambdaLayerJavaScriptSdkVersion.LATEST),
+        layerVersion: AdotLayerVersion.fromJavaScriptSdkLayerVersion(AdotLambdaLayerJavaScriptSdkVersion.V1_15_0_1),
         execWrapper: AdotLambdaExecWrapper.REGULAR_HANDLER,
       },
     });
@@ -42,7 +42,7 @@ class StackUnderTest extends Stack {
       handler: 'index.handler',
       code: Code.fromInline('def handler(event, context): pass'),
       adotInstrumentation: {
-        layerVersion: AdotLayerVersion.fromPythonSdkLayerVersion(AdotLambdaLayerPythonSdkVersion.LATEST),
+        layerVersion: AdotLayerVersion.fromPythonSdkLayerVersion(AdotLambdaLayerPythonSdkVersion.V1_19_0_1),
         execWrapper: AdotLambdaExecWrapper.INSTRUMENT_HANDLER,
       },
     });
@@ -52,7 +52,7 @@ class StackUnderTest extends Stack {
       handler: 'index.handler',
       code: Code.fromInline('def handler(event, context): pass'),
       adotInstrumentation: {
-        layerVersion: AdotLayerVersion.fromJavaSdkLayerVersion(AdotLambdaLayerJavaSdkVersion.LATEST),
+        layerVersion: AdotLayerVersion.fromJavaSdkLayerVersion(AdotLambdaLayerJavaSdkVersion.V1_28_1),
         execWrapper: AdotLambdaExecWrapper.INSTRUMENT_HANDLER,
       },
     });
@@ -62,7 +62,7 @@ class StackUnderTest extends Stack {
       handler: 'index.handler',
       code: Code.fromInline('def handler(event, context): pass'),
       adotInstrumentation: {
-        layerVersion: AdotLayerVersion.fromJavaAutoInstrumentationLayerVersion(AdotLambdaLayerJavaAutoInstrumentationVersion.LATEST),
+        layerVersion: AdotLayerVersion.fromJavaAutoInstrumentationLayerVersion(AdotLambdaLayerJavaAutoInstrumentationVersion.V1_28_1),
         execWrapper: AdotLambdaExecWrapper.INSTRUMENT_HANDLER,
       },
     });
@@ -72,7 +72,7 @@ class StackUnderTest extends Stack {
       handler: 'index.handler',
       code: Code.fromInline('def handler(event, context): pass'),
       adotInstrumentation: {
-        layerVersion: AdotLayerVersion.fromGenericLayerVersion(AdotLambdaLayerGenericVersion.LATEST),
+        layerVersion: AdotLayerVersion.fromGenericLayerVersion(AdotLambdaLayerGenericVersion.V0_82_0),
         execWrapper: AdotLambdaExecWrapper.INSTRUMENT_HANDLER,
       },
     });
diff --git a/packages/aws-cdk-lib/aws-lambda/lib/adot-layers.ts b/packages/aws-cdk-lib/aws-lambda/lib/adot-layers.ts
index 4587d21117c93..2a8957fbc2b60 100644
--- a/packages/aws-cdk-lib/aws-lambda/lib/adot-layers.ts
+++ b/packages/aws-cdk-lib/aws-lambda/lib/adot-layers.ts
@@ -213,7 +213,12 @@ export class AdotLambdaLayerJavaSdkVersion extends AdotLambdaLayerVersion {
    * The latest layer version available in this CDK version. New versions could
    * introduce incompatible changes. Make sure to test them before deploying to production.
    */
-  public static readonly LATEST = new AdotLambdaLayerJavaSdkVersion('1.19.0');
+  public static readonly LATEST = new AdotLambdaLayerJavaSdkVersion('1.28.1');
+
+  /**
+   * Version 1.28.1
+   */
+  public static readonly V1_28_1 = new AdotLambdaLayerJavaSdkVersion('1.28.1');
 
   /**
    * Version 1.19.0
@@ -233,7 +238,12 @@ export class AdotLambdaLayerJavaAutoInstrumentationVersion extends AdotLambdaLay
    * The latest layer version available in this CDK version. New versions could
    * introduce incompatible changes. Make sure to test them before deploying to production.
    */
-  public static readonly LATEST = new AdotLambdaLayerJavaAutoInstrumentationVersion('1.19.2');
+  public static readonly LATEST = new AdotLambdaLayerJavaAutoInstrumentationVersion('1.28.1');
+
+  /**
+   * Version 1.28.1
+   */
+  public static readonly V1_28_1 = new AdotLambdaLayerJavaAutoInstrumentationVersion('1.28.1');
 
   /**
    * Version 1.19.2
@@ -253,7 +263,12 @@ export class AdotLambdaLayerPythonSdkVersion extends AdotLambdaLayerVersion {
    * The latest layer version available in this CDK version. New versions could
    * introduce incompatible changes. Make sure to test them before deploying to production.
    */
-  public static readonly LATEST = new AdotLambdaLayerPythonSdkVersion('1.18.0');
+  public static readonly LATEST = new AdotLambdaLayerPythonSdkVersion('1.19.0-1');
+
+  /**
+   * Version 1.19.0
+   */
+  public static readonly V1_19_0_1 = new AdotLambdaLayerPythonSdkVersion('1.19.0-1');
 
   /**
    * Version 1.18.0
@@ -298,7 +313,12 @@ export class AdotLambdaLayerJavaScriptSdkVersion extends AdotLambdaLayerVersion
    * The latest layer version available in this CDK version. New versions could
    * introduce incompatible changes. Make sure to test them before deploying to production.
    */
-  public static readonly LATEST = new AdotLambdaLayerJavaScriptSdkVersion('1.7.0');
+  public static readonly LATEST = new AdotLambdaLayerJavaScriptSdkVersion('1.15.0-1');
+
+  /**
+   * Version 1.15.0
+   */
+  public static readonly V1_15_0_1 = new AdotLambdaLayerJavaScriptSdkVersion('1.15.0-1');
 
   /**
    * Version 1.7.0
@@ -318,7 +338,12 @@ export class AdotLambdaLayerGenericVersion extends AdotLambdaLayerVersion {
    * The latest layer version available in this CDK version. New versions could
    * introduce incompatible changes. Make sure to test them before deploying to production.
    */
-  public static readonly LATEST = new AdotLambdaLayerGenericVersion('0.62.1');
+  public static readonly LATEST = new AdotLambdaLayerGenericVersion('0.82.0');
+
+  /**
+   * Version 0.82.0
+   */
+  public static readonly V0_82_0 = new AdotLambdaLayerGenericVersion('0.82.0');
 
   /**
    * Version 0.62.1
diff --git a/packages/aws-cdk-lib/aws-lambda/test/adot.test.ts b/packages/aws-cdk-lib/aws-lambda/test/adot.test.ts
index 1cf54eaa83856..a0eb210538463 100644
--- a/packages/aws-cdk-lib/aws-lambda/test/adot.test.ts
+++ b/packages/aws-cdk-lib/aws-lambda/test/adot.test.ts
@@ -21,10 +21,10 @@ describe('ADOT Lambda Layer', () => {
     });
 
     test('is added properly when the region information is available at synthesis time', () => {
-      const layerArn = lambda.AdotLambdaLayerJavaSdkVersion.V1_19_0.layerArn(fn.stack, fn.architecture);
+      const layerArn = lambda.AdotLambdaLayerJavaSdkVersion.V1_28_1.layerArn(fn.stack, fn.architecture);
 
       expect(layerArn).toEqual(
-        'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-19-0:1',
+        'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-28-1:1',
       );
     });
 
@@ -32,7 +32,7 @@ describe('ADOT Lambda Layer', () => {
       const layerArn = lambda.AdotLambdaLayerJavaSdkVersion.LATEST.layerArn(fn.stack, fn.architecture);
 
       expect(layerArn).toEqual(
-        'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-19-0:1',
+        'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-28-1:1',
       );
     });
   });
@@ -53,10 +53,10 @@ describe('ADOT Lambda Layer', () => {
     });
 
     test('is added properly when the region information is available at synthesis time', () => {
-      const layerArn = lambda.AdotLambdaLayerPythonSdkVersion.V1_18_0.layerArn(fn.stack, fn.architecture);
+      const layerArn = lambda.AdotLambdaLayerPythonSdkVersion.V1_19_0_1.layerArn(fn.stack, fn.architecture);
 
       expect(layerArn).toEqual(
-        'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-python-amd64-ver-1-18-0:2',
+        'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-python-amd64-ver-1-19-0:2',
       );
     });
 
@@ -64,7 +64,7 @@ describe('ADOT Lambda Layer', () => {
       const layerArn = lambda.AdotLambdaLayerPythonSdkVersion.LATEST.layerArn(fn.stack, fn.architecture);
 
       expect(layerArn).toEqual(
-        'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-python-amd64-ver-1-18-0:2',
+        'arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-python-amd64-ver-1-19-0:2',
       );
     });
   });
@@ -107,12 +107,15 @@ describe('ADOT Lambda Layer', () => {
       // THEN
       Template.fromStack(fn.stack).hasOutput('ExportLayerArn', {
         Value: {
-          'Fn::FindInMap': [
-            'AdotlambdalayerMap',
-            {
-              Ref: 'AWS::Region',
-            },
-            'JAVAxSDKx1x19x0xarm64',
+          'Fn::Join': [
+            '',
+            [
+              'arn:aws:lambda:',
+              {
+                Ref: 'AWS::Region',
+              },
+              ':901920570463:layer:aws-otel-java-wrapper-arm64-ver-1-28-1:1',
+            ],
           ],
         },
       });
diff --git a/packages/aws-cdk-lib/aws-lambda/test/function.test.ts b/packages/aws-cdk-lib/aws-lambda/test/function.test.ts
index 167b9fc5e3dd5..c3ee0e9a4e6a2 100644
--- a/packages/aws-cdk-lib/aws-lambda/test/function.test.ts
+++ b/packages/aws-cdk-lib/aws-lambda/test/function.test.ts
@@ -3061,14 +3061,14 @@ describe('function', () => {
       handler: 'index.handler',
       runtime: lambda.Runtime.NODEJS_14_X,
       adotInstrumentation: {
-        layerVersion: lambda.AdotLayerVersion.fromJavaSdkLayerVersion(AdotLambdaLayerJavaSdkVersion.V1_19_0),
+        layerVersion: lambda.AdotLayerVersion.fromJavaSdkLayerVersion(AdotLambdaLayerJavaSdkVersion.V1_28_1),
         execWrapper: lambda.AdotLambdaExecWrapper.REGULAR_HANDLER,
       },
     });
 
     // THEN
     Template.fromStack(stack).hasResourceProperties('AWS::Lambda::Function', {
-      Layers: ['arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-19-0:1'],
+      Layers: ['arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-java-wrapper-amd64-ver-1-28-1:1'],
       Environment: {
         Variables: {
           AWS_LAMBDA_EXEC_WRAPPER: '/opt/otel-handler',
@@ -3090,14 +3090,14 @@ describe('function', () => {
       handler: 'index.handler',
       runtime: lambda.Runtime.PYTHON_3_9,
       adotInstrumentation: {
-        layerVersion: lambda.AdotLayerVersion.fromPythonSdkLayerVersion(lambda.AdotLambdaLayerPythonSdkVersion.V1_13_0),
+        layerVersion: lambda.AdotLayerVersion.fromPythonSdkLayerVersion(lambda.AdotLambdaLayerPythonSdkVersion.V1_19_0_1),
         execWrapper: lambda.AdotLambdaExecWrapper.INSTRUMENT_HANDLER,
       },
     });
 
     // THEN
     Template.fromStack(stack).hasResourceProperties('AWS::Lambda::Function', {
-      Layers: ['arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-python-amd64-ver-1-13-0:1'],
+      Layers: ['arn:aws:lambda:us-west-2:901920570463:layer:aws-otel-python-amd64-ver-1-19-0:2'],
       Environment: {
         Variables: {
           AWS_LAMBDA_EXEC_WRAPPER: '/opt/otel-instrument',
@@ -3114,7 +3114,7 @@ describe('function', () => {
       handler: 'index.handler',
       runtime: lambda.Runtime.PYTHON_3_10,
       adotInstrumentation: {
-        layerVersion: lambda.AdotLayerVersion.fromPythonSdkLayerVersion(lambda.AdotLambdaLayerPythonSdkVersion.V1_13_0),
+        layerVersion: lambda.AdotLayerVersion.fromPythonSdkLayerVersion(lambda.AdotLambdaLayerPythonSdkVersion.V1_19_0_1),
         execWrapper: lambda.AdotLambdaExecWrapper.REGULAR_HANDLER,
       },
     })).toThrow(/Python Adot Lambda layer requires AdotLambdaExecWrapper.INSTRUMENT_HANDLER/);
@@ -3133,7 +3133,7 @@ describe('function', () => {
         new lambda.DockerImageFunction(stack, 'MyLambda', {
           code: lambda.DockerImageCode.fromImageAsset(dockerLambdaHandlerPath),
           adotInstrumentation: {
-            layerVersion: lambda.AdotLayerVersion.fromJavaSdkLayerVersion(AdotLambdaLayerJavaSdkVersion.V1_19_0),
+            layerVersion: lambda.AdotLayerVersion.fromJavaSdkLayerVersion(AdotLambdaLayerJavaSdkVersion.V1_28_1),
             execWrapper: lambda.AdotLambdaExecWrapper.REGULAR_HANDLER,
           },
         }),