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Phylogenetic tree generation #216

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jeffreybarrick opened this issue Sep 27, 2019 · 2 comments
Open

Phylogenetic tree generation #216

jeffreybarrick opened this issue Sep 27, 2019 · 2 comments

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@jeffreybarrick
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Update code for making phylogenetic trees via gdtools PHYLOGENY and then generating graphics. This would be useful specifically for adding to the LTEE-Ecoli website.

https://github.com/barricklab/LTEE-Ecoli

@jeffreybarrick
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Notes from today:

  • One can currently run gdtools PHYLOGENY to get a PHYLIP formatted file to analyze as an example. Given the limitations in terms of name length of PHYLIP format and not wanting to force a user to rely on dnapars for tree generation, we should output a different phylogenetic alignment format from gdtools COMPARE.
  • A good place for example data is https://github.com/barricklab/LTEE-Ecoli. The GD files are in LTEE-clone-curated and the reference file is in reference. One should be able to reproduce the trees in this paper using these data:
  • Some requested functionality
    • Coloring genomes and using symbols in a standardized way based on a metadata file describing treatments, populations, etc.
    • Detect and automatically re-scale long branches (such as those caused by mutators).
    • Root the tree based on an ancestral genome.

@jeffreybarrick
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One can now generate a multi-FASTA genotype file without any limitations on name length:

gdtools COMPARE -f FASTA

Recommend using this in any packages/pipelines for performing phylogenetic analyses.

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