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additional mock communities to include #26

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gregcaporaso opened this issue Jun 23, 2016 · 5 comments
Closed
2 tasks done

additional mock communities to include #26

gregcaporaso opened this issue Jun 23, 2016 · 5 comments

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@gregcaporaso
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gregcaporaso commented Jun 23, 2016

From Robert Edgar (via feedback on the PeerJ pre-print):

  • Kozich et al. "dual indexing" V4 HMP mock (doi: 10.1128/AEM.01043-13, FASTQ files: http://www.mothur.org/MiSeqDevelopmentData.html). This is yet another HMP V4 run, but is useful because it has a quite different error profile (presumably due to the different library preparation method).
  • The "Extreme" community used for DADA2 validation (doi:10.1038/nmeth.3869, SRA run id SRR2990088). This is useful because it has several species which are >97% similar so cannot be resolved by traditional OTU methods.
@gregcaporaso gregcaporaso changed the title additional mock communities to add additional mock communities to include Jun 23, 2016
@nbokulich
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These datasets do not have barcode files. Should we change the travisCI requirements to eliminate the barcode file requirement?

@gregcaporaso
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Should we change the travisCI requirements to eliminate the barcode file requirement?

Yes, I think so. Can we adapt the requirement in some way so there is either a barcodes file or some other scenario (I'm not able to download the files at the moment due to slow wifi connection, so I don't yet have a suggestion for what that requirement could be).

@nbokulich
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Thanks! perhaps we can require a valid barcodes fp if barcodes are listed in the mapping file, or if > 1 sample is listed in the mapping file.

@gregcaporaso
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That should work. Want to make that change? I can review tomorrow when I add the others to Dropbox.

@nbokulich
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Both of these communities (and others) were added with PR #52 . Resolving.

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