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Annotation(UniProt) vs ProteinGroup(UniParc) #108

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aarango1 opened this issue Aug 9, 2024 · 0 comments
Open

Annotation(UniProt) vs ProteinGroup(UniParc) #108

aarango1 opened this issue Aug 9, 2024 · 0 comments
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@aarango1
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aarango1 commented Aug 9, 2024

Describe the bug
I am not able to observe the annotations in my matrix.
I am using
As annotation file:
mainAnnot.escherichia_albertii_nbrc_107761_=_dsm_17582
mainAnnot.klebsiella_pneumoniae_subsp.pneumoniae(strain_atcc_700721___mgh_78578)

As FASTA file
uniparc_upid_UP000029463_2024_07_20Escherichia albertii NBRC 107761 = DSM 17582
JSZI01_Klebsiella pneumoniae subsp. pneumoniae_ATCC 13883

I know there is some information missing but I guess the problem has to do with the format that the annotation step is expecting to find (as UniProt) and In my proteingroups file (from MaxQuant) I have UniParc ID since my FASTA files also has UniParc IDs.

Is there any way to display the annotations in my matrix?
(I do not have FASTA files with Uniprot IDs for the strains that I need)

To Reproduce
Once the .txt file is downloaded and added to ...\Perseus_v2.1.1.0\bin\conf\annotations
Click on add annotation and select the file

image

Expected behavior
I expected to observe all the annotations that I selected as columns in my matrix, but the are empty.
For example gene name or Uniprot ID (Albertii gene name annotation column is empty but not for Klebsiella)
image

Screenshots
This is how my proteingroup file looks like
image
image

Desktop (please complete the following information):
MaxQuant 2.6.2
Perseus 2.1.1
Windows 11 Enterprise

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