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General stats and bcl2fastq #1094

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nservant opened this issue Jan 9, 2020 · 5 comments · Fixed by #1107
Closed

General stats and bcl2fastq #1094

nservant opened this issue Jan 9, 2020 · 5 comments · Fixed by #1107

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@nservant
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nservant commented Jan 9, 2020

Hi @ewels,
We have a different behavior in the multiqc report using version 1.6dev and 1.7/1.8, and the bcl2fastq module. The difference occurs in the general stats table.

We have a single sample, with 4 barcodes.
Using multiqc 1.6dev, the general stats table reported the 4 barcodes independantly.

image

With the multiqc 1.7 and 1.8, the barcodes are merged (as the belong to the same sample), but the number are false. It display the last barcodes only.

image

Did you already observed that before ? and would have any idea of what's going wrong ?
Many thanks

@nservant
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nservant commented Jan 9, 2020

I'm adding the bcl2fastq zip files if it helps
Stats.json.zip

@matrulda
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I've encountered this problem too and found a bug in the code.
https://github.com/ewels/MultiQC/blob/964e9993505c64ad95a7914a8c4f60eed499aa0d/multiqc/modules/bcl2fastq/bcl2fastq.py#L177
It should compare SampleName with SampleId, not SampleName with SampleName which always is true. I'll submit at PR to fix this.

@nservant
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wonderful ! Thanks

@ewels
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ewels commented Feb 16, 2020

Sorry for the slow response @nservant and thanks for the fix @matrulda - now merged so should be fixed in the dev version 👍🏻

@matrulda
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No problem! :)

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3 participants