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Samtools Idxstats - Additional Normalisation Plot #1118

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StephenRicher opened this issue Mar 5, 2020 · 3 comments
Closed

Samtools Idxstats - Additional Normalisation Plot #1118

StephenRicher opened this issue Mar 5, 2020 · 3 comments

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@StephenRicher
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StephenRicher commented Mar 5, 2020

Hi,

First off thanks very much for this fantastic tool! I was wondering if it would it be possible to represent the samtools idxstats output with an additional plot normalised by chromosome/contig length and by total read count?

As I understand the current normalised count is normalised by sequencing depth for comparison between samples.

However, I think it would be really cool to have an additional normalisation plot that also normalises by contig length in addition to total read count. This would enable comparison between samples but also enable a quick assessment of any contigs that have higher coverage than expected.

Thanks and I look forward to hearing your thoughts,

Stephen

@ewels
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ewels commented Mar 29, 2020

Hi @StephenRicher,

I think this is a great idea! We already have a plot normalising by total read count, so I guess you mean normalising both of those measures at once? Essentially adding a third plot tab here:

image

I agree that this would be more helpful as it's typically what people are looking for in this plot 👍

Fancy having a stab at it? 😉

Phil

@StephenRicher
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Hi Phil,

Thanks very much for your email, sure I am happy to have a go! :)

Thanks,
Stephen

@StephenRicher StephenRicher mentioned this issue Mar 29, 2020
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@ewels
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ewels commented Apr 30, 2020

Closed in #1140

@ewels ewels closed this as completed Apr 30, 2020
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