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MultiQC Version 1.0
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@ewels ewels released this 17 May 16:04
v1.0
a63c879

Version 1.0! This release has been a long time coming and brings with it some fairly
major improvements in speed, report filesize and report performance. There's also
a bunch of new modules, more options, features and a whole lot of bug fixes.

The version number is being bumped up to 1.0 for a couple of reasons:

  1. MultiQC is now (hopefully) relatively stable. A number of facilities and users
    are now using it in a production setting and it's published. It feels like it
    probably deserves v1 status now somehow.
  2. This update brings some fairly major changes which will break backwards
    compatibility for plugins. As such, semantic versioning suggests a change in
    major version number.

Breaking Changes

For most people, you shouldn't have any problems upgrading. There are two
scenarios where you may need to make changes with this update:

1. You have custom file search patterns

Search patterns have been flattened and may no longer have arbitrary depth.
For example, you may need to change the following:

fastqc:
    data:
        fn: 'fastqc_data.txt'
    zip:
        fn: '*_fastqc.zip'

to this:

fastqc/data:
    fn: 'fastqc_data.txt'
fastqc/zip:
    fn: '*_fastqc.zip'

See the documentation for instructions on how to write the new file search syntax.

See search_patterns.yaml for the new module search keys
and more examples.

2. You have custom plugins / modules / external code

To see what changes need to applied to your custom plugin code, please see the MultiQC docs.

Module updates:

  • Adapter Removal - new module!
    • AdapterRemoval v2 - rapid adapter trimming, identification, and read merging
  • BUSCO - new module!
    • New module for the BUSCO v2 tool, used for assessing genome assembly and annotation completeness.
  • Cluster Flow - new module!
    • Cluster Flow is a workflow tool for bioinformatics pipelines. The new module parses executed tool commands.
  • RNA-SeQC - new module!
    • New module to parse output from RNA-SeQC, a java program which computes a series
      of quality control metrics for RNA-seq data.
  • goleft indexcov - new module! Thanks to @chapmanb and @brentp
    • goleft indexcov uses the PED and ROC
      data files to create diagnostic plots of coverage per sample, helping to identify sample gender and coverage issues.
  • SortMeRNA - new module! Written by @bschiffthaler
    • New module for SortMeRNA, commonly used for removing rRNA contamination from datasets.
  • Bcftools
    • Fixed bug with display of indels when only one sample
  • Cutadapt
    • Now takes the filename if the sample name is - (stdin). Thanks to @tdido
  • FastQC
    • Data for the Sequence content plot can now be downloaded from reports as a JSON file.
  • FastQ Screen
    • Rewritten plotting method for high sample numbers plot (~ > 20 samples)
    • Now shows counts for single-species hits and bins all multi-species hits
    • Allows plot to show proper percentage view for each sample, much easier to interpret.
  • HTSeq
    • Fix bug where header lines caused module to crash
  • Picard
    • New RrbsSummaryMetrics Submodule!
    • New WgsMetrics Submodule!
    • CollectGcBiasMetrics module now prints summary statistics to multiqc_data if found. Thanks to @ahvigil
  • Preseq
    • Now trims the x axis to the point that meets 90% of min(unique molecules).
      Hopefully prevents ridiculous x axes without sacrificing too much useful information.
    • Allows to show estimated depth of coverage instead of less informative molecule counts
      (see details).
    • Plots dots with externally calculated real read counts (see details).
  • Qualimap
    • RNASeq Transcript Profile now has correct axis units. Thanks to @roryk
    • BamQC module now doesn't crash if reports don't have genome gc distributions
  • RSeQC
    • Fixed Python3 error in Junction Saturation code
    • Fixed JS error for Junction Saturation that made the single-sample combined plot only show All Junctions

Core MultiQC updates:

  • Change in module structure and import statements (see details).
  • Module file search has been rewritten (see above changes to configs)
    • Significant improvement in search speed (test dataset runs in approximately half the time)
    • More options for modules to find their logs, eg. filename and contents matching regexes (see the docs)
  • Report plot data is now compressed, significantly reducing report filesizes.
  • New --ignore-samples option to skip samples based on parsed sample name
    • Alternative to filtering by input filename, which doesn't always work
    • Also can use config vars sample_names_ignore (glob patterns) and sample_names_ignore_re (regex patterns).
  • New --sample-names command line option to give file with alternative sample names
    • Allows one-click batch renaming in reports
  • New --cl_config option to supply MultiQC config YAML directly on the command line.
  • New config option to change numeric multiplier in General Stats
    • For example, if reports have few reads, can show Thousands of Reads instead of Millions of Reads
    • Set config options read_count_multiplier, read_count_prefix and read_count_desc
  • Config options decimalPoint_format and thousandsSep_format now apply to tables as well as plots
    • By default, thosands will now be separated with a space and . used for decimal places.
  • Tables now have a maximum-height by default and scroll within this.
    • Speeds up report rendering in the web browser and makes report less stupidly long with lots of samples
    • Button beneath table toggles full length if you want a zoomed-out view
    • Refactored and removed previous code to make the table header "float"
    • Set config.collapse_tables to False to disable table maximum-heights
  • Bar graphs and heatmaps can now be zoomed in on
    • Interactive plots sometimes hide labels due to lack of space. These can now be zoomed in on to see specific samples in more detail.
  • Report plots now load sequentially instead of all at once
    • Prevents the browser from locking up when large reports load
  • Report plot and section HTML IDs are now sanitised and checked for duplicates
  • New template available (called sections) which has faster loading
    • Only shows results from one module at a time
    • Makes big reports load in the browser much more quickly, but requires more clicking
    • Try it out by specifying -t sections
  • Module sections tidied and refactored
    • New helper function self.add_section()
    • Sections hidden in nav if no title (no more need for the hacky self.intro += )
    • Content broken into description, help and plot, with automatic formatting
    • Empty module sections are now skipped in reports. No need to check if a plot function returns None!
    • Changes should be backwards-compatible
  • Report plot data export code refactored
    • Now doesn't export hidden samples (uses HighCharts export-csv plugin)
  • Handle error when git isn't installed on the system.
  • Refactored colouring of table cells
    • Was previously done in the browser using chroma.js
    • Now done at report generation time using the spectra package
    • Should helpfully speed up report rendering time in the web browser, especially for large reports
  • Docs updates (thanks to @varemo)
  • Previously hidden log file .multiqc.log renamed to multiqc.log in multiqc_data
  • Added option to load MultiQC config file from a path specified in the environment variable MULTIQC_CONFIG_PATH
  • New table configuration options
    • sortRows: False prevents table rows from being sorted alphabetically
    • col1_header allows the default first column header to be changed from "Sample Name"
  • Tables no longer show Configure Columns and Plot buttons if they only have a single column
  • Custom content updates
    • New custom_content/order config option to specify order of Custom Content sections
    • Tables now use the header for the first column instead of always having Sample Name
    • JSON + YAML tables now remember order of table columns
    • Many minor bugfixes
  • Line graphs and scatter graphs axis limits
    • If limits are specified, data exceeding this is no longer saved in report
    • Visually identical, but can make report file sizes considerable smaller in some cases
  • Creating multiple plots without a config dict now works (previously just gave grey boxes in report)
  • All changes are now tested on a Windows system, using AppVeyor
  • Fixed rare error where some reports could get empty General Statistics tables when no data present.
  • Fixed minor bug where config option force: true didn't work. Now you don't have to always specify -f!
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