You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
I am getting the following errors when attempting to clean my raw-data reads (see below). I have double checked the formatting of my file and wonder if the dash in the raw read files that I want to rename are what is mucking things up, or maybe another issue my tired eyes cannot see. I have attached my config file and pasted my SH below the error message. Additionally, I should mention I get the same error when working through the tutorial. I am using phyluce v 1.7.3 and accessing the university hpcc via Windows Powershell. My config and shell script are in my "scratch" with a folder for the raw reads.
2024-03-16 23:01:33,467 - illumiprocessor - INFO - ==================== Starting illumiprocessor ===================
2024-03-16 23:01:33,469 - illumiprocessor - INFO - Version: 2.10
2024-03-16 23:01:33,469 - illumiprocessor - INFO - Argument --config: illumiprocessor3.conf
2024-03-16 23:01:33,469 - illumiprocessor - INFO - Argument --cores: 4
2024-03-16 23:01:33,469 - illumiprocessor - INFO - Argument --input: /scratch/jr4253/raw_fastq/raw_fastq
2024-03-16 23:01:33,470 - illumiprocessor - INFO - Argument --log_path: None
2024-03-16 23:01:33,470 - illumiprocessor - INFO - Argument --min_len: 40
2024-03-16 23:01:33,470 - illumiprocessor - INFO - Argument --no_merge: False
2024-03-16 23:01:33,470 - illumiprocessor - INFO - Argument --output: /scratch/jr4253/raw_fastq/clean_fastq
2024-03-16 23:01:33,470 - illumiprocessor - INFO - Argument --phred: phred33
2024-03-16 23:01:33,470 - illumiprocessor - INFO - Argument --r1_pattern: None
2024-03-16 23:01:33,471 - illumiprocessor - INFO - Argument --r2_pattern: None
2024-03-16 23:01:33,471 - illumiprocessor - INFO - Argument --se: False
2024-03-16 23:01:33,471 - illumiprocessor - INFO - Argument --trimmomatic: /home/jr4253/.conda/envs/phyluce-1.7.3/bin/trimmomatic
2024-03-16 23:01:33,471 - illumiprocessor - INFO - Argument --verbosity: INFO
Traceback (most recent call last):
File "/home/jr4253/.conda/envs/phyluce-1.7.3/lib/python3.6/configparser.py", line 846, in items
d.update(self._sections[section])
KeyError: 'names'
During handling of the above exception, another exception occurred:
Traceback (most recent call last):
File "/home/jr4253/.conda/envs/phyluce-1.7.3/bin/illumiprocessor", line 17, in
sys.exit(main())
File "/home/jr4253/.conda/envs/phyluce-1.7.3/lib/python3.6/site-packages/illumiprocessor/cli/main.py", line 114, in main
main(args)
File "/home/jr4253/.conda/envs/phyluce-1.7.3/lib/python3.6/site-packages/illumiprocessor/main.py", line 33, in main
for start_name, end_name in conf.items("names"):
File "/home/jr4253/.conda/envs/phyluce-1.7.3/lib/python3.6/configparser.py", line 849, in items
raise NoSectionError(section)
configparser.NoSectionError: No section: 'names'
SH
#!/bin/bash
#SBATCH --job-name=UCE_clean # Job name
#SBATCH --mail-type=BEGIN,END,FAIL # Mail events (NONE, BEGIN, END, FAIL, ALL)
#SBATCH --mail-user=jr4253@nau.edu # Where to send mail
#SBATCH --nodes=1-1 # Run a single task
#SBATCH --ntasks-per-node=16 # Number of CPU cores per task
#SBATCH --mem=80gb # Job memory request
#SBATCH --time=06-00:00:00 # Time limit days-hrs:min:sec
#SBATCH --output=parallel_%j.log # Standard output and error log
#SBATCH --chdir=/scratch/jr4253
It looks like your script might be putting you into the wrong directory - the cd command is placing you in /scratch/jr4253/raw_fastq - do you have raw_fastq folder nested inside of that (so like /scratch/jr4253/raw_fastq/raw_fastq?
Currently, I have my SH and config in my scratch (/scratch/jr4253) and my raw reads are in the raw reads folder inside of that directory (/scratch/jr4253/raw_fatsq) but I do not have another folder within raw_fastq. So perhaps that is the error (fingers crossed!) and changing my cd to /scratch/jr4253 will help? I'll try this shortly and get back to you.
I have since updated my SH file and noticed I had some raw reads not accounted for in my config file. Omitting those (they're not needed now) and updating my SH worked!
Hello Dr. Faircloth,
I am getting the following errors when attempting to clean my raw-data reads (see below). I have double checked the formatting of my file and wonder if the dash in the raw read files that I want to rename are what is mucking things up, or maybe another issue my tired eyes cannot see. I have attached my config file and pasted my SH below the error message. Additionally, I should mention I get the same error when working through the tutorial. I am using phyluce v 1.7.3 and accessing the university hpcc via Windows Powershell. My config and shell script are in my "scratch" with a folder for the raw reads.
illumiprocessor3.txt
Thank you in advance,
Jamison
parallel file with error code:
2024-03-16 23:01:33,467 - illumiprocessor - INFO - ==================== Starting illumiprocessor ===================
2024-03-16 23:01:33,469 - illumiprocessor - INFO - Version: 2.10
2024-03-16 23:01:33,469 - illumiprocessor - INFO - Argument --config: illumiprocessor3.conf
2024-03-16 23:01:33,469 - illumiprocessor - INFO - Argument --cores: 4
2024-03-16 23:01:33,469 - illumiprocessor - INFO - Argument --input: /scratch/jr4253/raw_fastq/raw_fastq
2024-03-16 23:01:33,470 - illumiprocessor - INFO - Argument --log_path: None
2024-03-16 23:01:33,470 - illumiprocessor - INFO - Argument --min_len: 40
2024-03-16 23:01:33,470 - illumiprocessor - INFO - Argument --no_merge: False
2024-03-16 23:01:33,470 - illumiprocessor - INFO - Argument --output: /scratch/jr4253/raw_fastq/clean_fastq
2024-03-16 23:01:33,470 - illumiprocessor - INFO - Argument --phred: phred33
2024-03-16 23:01:33,470 - illumiprocessor - INFO - Argument --r1_pattern: None
2024-03-16 23:01:33,471 - illumiprocessor - INFO - Argument --r2_pattern: None
2024-03-16 23:01:33,471 - illumiprocessor - INFO - Argument --se: False
2024-03-16 23:01:33,471 - illumiprocessor - INFO - Argument --trimmomatic: /home/jr4253/.conda/envs/phyluce-1.7.3/bin/trimmomatic
2024-03-16 23:01:33,471 - illumiprocessor - INFO - Argument --verbosity: INFO
Traceback (most recent call last):
File "/home/jr4253/.conda/envs/phyluce-1.7.3/lib/python3.6/configparser.py", line 846, in items
d.update(self._sections[section])
KeyError: 'names'
During handling of the above exception, another exception occurred:
Traceback (most recent call last):
File "/home/jr4253/.conda/envs/phyluce-1.7.3/bin/illumiprocessor", line 17, in
sys.exit(main())
File "/home/jr4253/.conda/envs/phyluce-1.7.3/lib/python3.6/site-packages/illumiprocessor/cli/main.py", line 114, in main
main(args)
File "/home/jr4253/.conda/envs/phyluce-1.7.3/lib/python3.6/site-packages/illumiprocessor/main.py", line 33, in main
for start_name, end_name in conf.items("names"):
File "/home/jr4253/.conda/envs/phyluce-1.7.3/lib/python3.6/configparser.py", line 849, in items
raise NoSectionError(section)
configparser.NoSectionError: No section: 'names'
SH
#!/bin/bash
#SBATCH --job-name=UCE_clean # Job name
#SBATCH --mail-type=BEGIN,END,FAIL # Mail events (NONE, BEGIN, END, FAIL, ALL)
#SBATCH --mail-user=jr4253@nau.edu # Where to send mail
#SBATCH --nodes=1-1 # Run a single task
#SBATCH --ntasks-per-node=16 # Number of CPU cores per task
#SBATCH --mem=80gb # Job memory request
#SBATCH --time=06-00:00:00 # Time limit days-hrs:min:sec
#SBATCH --output=parallel_%j.log # Standard output and error log
#SBATCH --chdir=/scratch/jr4253
module load anaconda3
conda activate phyluce-1.7.3
cd /scratch/jr4253/raw_fastq
illumiprocessor
--input raw_fastq/
--output clean_fastq
--config illumiprocessor3.conf
--cores 4
The text was updated successfully, but these errors were encountered: