+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 3
+
+
+
+
+
+ 3
+
+
+
+
+
+ 20
+
+
+
+
+
+
+
+ 3
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 2
+
+
+
+
+
+
+
+
+
+
+
+ 281
+
+
+
+
+
+ 310
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Combined with the established role of the Wnt signaling pathway in adult hippocampal neurogenesis together with the known association between Lef1 and beta-catenin, our results here suggest that Lef1 is an important part of the Wnt controlled regulation of neural precursor function in the adult dentate gyrus."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25565948
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In vertebrates, the conserved Wnt signalling cascade promotes the stabilization and nuclear accumulation of β-catenin, which then associates with the lymphoid enhancer factor/T cell factor proteins (LEF/TCFs) to activate target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20126258
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Because it has been reported that LEF-1 interacts with beta-catenin outside the amino-terminal domain, namely, in the middle part of the molecule, the possible participation of beta-catenin has not been formally ruled out."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29998192
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Despite the finding that transcription factor LEF-1 interacts with beta-catenin to activate their target genes, such as EMT related gene SNAI2 XREF_BIBR, and induces EMT, we found that not only full-length LEF-1 but also LEF-1 lacking the N-terminal beta-catenin-binding domain can induce EMT."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29998192
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Undegraded beta-catenin in SPNs forms a complex with LEF1, enters the nucleus and activates transcription of several oncogenes including cyclin-D1 that is overexpressed in 70-100% of SPNs [XREF_BIBR, XREF_BIBR, XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27267993
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Because it has been reported that LEF-1 interacts with β-catenin outside the amino-terminal domain, namely, in the middle part of the molecule, the possible participation of β-catenin has not been formally ruled out."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29998192
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Nonetheless, there is a report that in addition to the well-established amino-terminal β-catenin–binding domain of LEF-1, β-catenin binds to another site of LEF-1: residues 150–175 xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29998192
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In our previous study, we have reported that not only wild-type LEF-1 but also an amino terminus–deleted mutant LEF-1, ΔNLEF-1, which cannot interact with β-catenin, has an ability to induce EMT xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29998192
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Despite the finding that transcription factor LEF-1 interacts with β-catenin to activate their target genes, such as EMT-related gene SNAI2 xref , and induces EMT, we found that not only full-length LEF-1 but also LEF-1 lacking the N-terminal β-catenin–binding domain can induce EMT."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29998192
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the nucleus, beta-catenin binds TCF and LEF1 transcription factors and drives the expression of specific target genes that control cellular processes ranging from proliferation to cell differentiation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25124032
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "ILK has been shown to regulate cell fate via nuclear interaction of beta-catenin with lymphoid enhancer factor (LEF-1) transcription factors."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 15673299
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Given the ability of DeltaNDeltaCbetacatenin to act in a dominant negative fashion in interfering with the activity of functional Lef1 and betacatenin complexes in keratinocytes in vitro, we then turned towards using this transgene to assess the consequences of diminished Wnt signaling on hair organogenesis and postnatal cycling."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12135986
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "These structures were accompanied by aberrant TOPGAL activation, reflective of functional Lef1 and beta-catenin complexes (XREF_FIG C)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12135986
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In addition, CK2 has been shown to regulate the interaction and transactivation activity of a beta-catenin and LEF -1 complex through phosphorylation of LEF-1 at S42, S61 [XREF_BIBR; XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19565571
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Beta-catenin forms complexes with Tcf and Lef-1 and functions as a transcriptional activator downstream of the Wnt signaling pathway."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9721853
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The Lef1, high mobility group (HMG) family transcription factor, interacts with beta-catenin and is a nuclear effector of Wnt1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26537192
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Matrix metalloproteinase (s) mediate (s) NO induced dissociation of beta-catenin from membrane bound E-cadherin and formation of nuclear beta-catenin and LEF -1 complex."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12507936
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The use of this type of library has identified erythropoieitin (EPO) mimetics (promote EPO receptor dimerization), inhibitors of Myc and Max dimerization, inhibitors of LEF-1 and beta-catenin dimerization, inhibitors of the binding of the protease MMP-2 to alphavbeta3 integrin, and inhibitors of HIV protease."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21183197
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The association of Ep-ICD with FHL2 and Wnt pathway components - β-catenin and Lef-1 forms a nuclear complex that binds DNA at Lef-1 consensus sites and induces gene transcription, leading to increased cell proliferation and has been shown to be oncogenic in immunodeficient mice [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20579375
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the nucleus, beta-catenin binds to TCF-4 and LEF-1 to produce a transcription factor complex, which mediates cell proliferation and epithelial-mesenchymal transition (EMT) by influencing genes [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 30867651
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The coordinated binding of beta-catenin and LEF-1 to enhancers is reminiscent of the cooperative binding of recombinant LEF-1 and beta-catenin proteins on chromatin templates in vitro."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25936800
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Because Wnt3a preferentially induces the full-length form of LEF-1, which includes the N-terminal beta-catenin interaction domain, this mechanism ensures mutually interdependent binding of LEF-1 and beta-catenin to chromatin and enables rapid and synchronous activation of downstream target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25936800
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Beta-catenin is then accumulated in the cytoplasm and translocated to the nucleus and binds to transcription factors LEF-1 and TCF to regulate the transcription of downstream target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22172041
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We conclude that Wnt3a signaling stimulates the robust assembly of LEF-1:β-catenin complexes near hESC mesendodermal genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25936800
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "As expected, Wnt3a greatly increased binding of both LEF-1 and β-catenin, predominantly to pre-existing enhancer sites."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25936800
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The coordinated binding of β-catenin and LEF-1 to enhancers is reminiscent of the cooperative binding of recombinant LEF-1 and β-catenin proteins on chromatin templates in vitro ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25936800
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Because Wnt3a preferentially induces the full-length form of LEF-1 ( xref ), which includes the N-terminal β-catenin interaction domain, this mechanism ensures mutually interdependent binding of LEF-1 and β-catenin to chromatin and enables rapid and synchronous activation of downstream target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25936800
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the nucleus β-catenin associates with members of the Lymphoid Enhancing Factor/ T-cell Factor (LEF/TCF) family of transcription factors, and induces the expression of genes that regulate cell proliferation, differentiation, migration, genetic stability and apoptosis [57–60] ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27090017
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Because EpICD has been shown to form β-catenin-mediated transcription complexes with LEF1 that is also overexpressed in endometrial tumors ( xref ), we examined LEF1 binding profiles in RL95-2 cells by ChIP-seq ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27569206
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "These results suggest that accumulated beta-catenin proteins may bind to the lymphocyte enhancer binding factor-1 (LEF-1), form the beta-catenin and LEF -1 complex, and stimulate such promoters regulating the cell cycle as the cyclin D1 gene."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12375019
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Interestingly, both the N- and C-terminal domains of TIEG1 were shown to interact with Lef1 and β-catenin using Duolink assays (Figure xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28201653
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In nuclei, β-catenin associates with the T-cell factor (TCF) and lymphoid enhancer factor (LEF) to induce transcription of target genes xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25170755
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "No binding of LEF-1 or β-catenin was observed at distal (5 kB) upstream sites (data not shown)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17113388
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The Arm R10-12 sequence facilitated transport even when beta-catenin was bound to the Arm binding partner LEF-1, and its activity was stimulated by phosphorylation at Tyr 654."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22110128
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The Arm R10-12 sequence facilitated transport even when β-catenin was bound to the Arm-binding partner LEF-1, and its activity was stimulated by phosphorylation at Tyr-654."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22110128
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "These results agree with the currently-accepted model that β-catenin associates with DNA-bound LEF1 and functions as a scaffold protein to recruit additional coactivators, such as CARM1 and p300, each of which makes a unique and specific contribution to the highly complex transcriptional initiation process."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21478268
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Mammalian Dpr1 and Dact inhibits expression of Wnt responsive reporters through its promotion of Dvl degradation in a lysosome dependent pathway, and by inhibiting the binding of LEF1 with beta-catenin, but promoting the binding of LEF1 with a corepressor, histone deacetylase 1 (HDAC1) XREF_BIBR, XREF_BIBR."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19440376
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Mammalian Dpr1/Dact inhibits expression of Wnt-responsive reporters through its promotion of Dvl degradation in a lysosome-dependent pathway, and by inhibiting the binding of LEF1 with β-catenin, but promoting the binding of LEF1 with a corepressor, histone deacetylase 1 (HDAC1) xref , xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19440376
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We conclude that residue F660 is essential for ICAT anchoring but plays no role in the β-catenin interaction with LEF1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28273108
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Additionally, β-catenin interacts with Lef-1 and with Klf4 to activate tert gene transcription."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25262238
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "beta-Catenin is an important partner in LEF1 transcriptional factor activity, and when LEF1 is bound to beta-catenin, LEF1 transports it to the nucleus."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 14691138
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "If beta-catenin is bound with LEF1, TGFbeta may not be able to dissociate the complex."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 14691138
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the presence of WNT signals, LEF1 binds to beta-catenin via its N-terminal beta-catenin-binding domain and promotes context dependent target gene transcription including c-MYC and AXIN2."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27965462
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "β-Catenin is an important partner in LEF1 transcriptional factor activity, and when LEF1 is bound to β-catenin, LEF1 transports it to the nucleus ( xref ; xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 14691138
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Interaction of LEF1 with nuclear β-catenin is known to effect Wnt signaling by stimulating the activity of LEF1/TCF transcription factors ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 14691138
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "If β-catenin is bound with LEF1, TGFβ may not be able to dissociate the complex ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 14691138
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The significance of this was poorly understood until yeast two hybrid studies revealed that the architectural HMG-box transcription factors LEF-1 and XTCF-3 interact with β-catenin ( Behrens et al., 1996; Molenaar et al., 1996 )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10495268
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the presence of WNT signals, LEF1 binds to β-catenin via its N-terminal β-catenin-binding domain and promotes context-dependent target gene transcription including c-MYC and AXIN2 ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27965462
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Since these RMS cell lines express the full-length LEF1 isoform of 44 kDa, whereas the truncated isoform lacking the β-catenin binding site (31 kDa and 23 kDa; see [ xref , xref ]) were barely detected (Figure xref ), we conclude that these cell lines express functional LEF1 that can interact with β-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27965462
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In some cell types, but not in others, activated beta-catenin interacts with members of the lymphoid enhancer-binding factor (LEF)/T-cell factor (TCF) family of transcription factors and induces gene expression."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 11750994
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Nuclear β-catenin associates with the lymphoid enhancer factor/T-cell factor (LEF/TCF) family of transcription factors to induce expression of genes involved in cell proliferation and survival, as well as migration and angiogenesis ( xref , xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25228654
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "XREF_BIBR, XREF_BIBR TGFbeta mediated PI3K-Akt signaling upregulates the transcription factor Snail and induces phosphorylation and inhibition of GSK-3beta which leads to assembly of LEF-1 and beta-Catenin complexes and upregulation of EMT promoting genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19471120
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Behrans J, van Kries JP, Kuehl M, Bruhn L, Wedlich
+D, o Grosschedl R, Birchmeier W: Functional interaction of beta-
+catenin with the transcription factor LEF-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9330865
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "As a result, there is cytoplasmic accumulation and nuclear translocation of beta-catenin, where it binds to LEF-1 and TCF, which in turn results in fibronectin induction."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21133803
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Together, the data obtained so far suggested that estradiol might activate a beta-Catenin and LEF -1 complex that is similar to that activated by Wnt in other systems."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19360103
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We observed that association of β-catenin with the LEF-1 promoter was diminished upon FoxM1 depletion ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22014570
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Thus, the decreased association of β-catenin with the LEF-1 promoter upon FoxM1 depletion was probably because both the nuclear β-catenin level ( xref ) and the binding between β-catenin and TCF4 were decreased ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22014570
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Active beta-catenin is known to translocate to the nucleus and interact with LEF1 to upregulate the expression of canonical target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26473529
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Epistasis analysis suggested that TRABID might act downstream of beta-catenin stabilization and affect the interaction of beta-catenin with LEF1 [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22584113
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This TFBS was identified by virtue of its interaction with the beta-catenin-T cell-factor/lymphoid-enhancer-factor complex (Tcf/Lef) [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 18485226
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "As a result it has been further shown that beta-catenin interacts with the transcription factor LEF1, which is in turn associated with Smad 4, thereby regulating the transcription of TGF-beta target molecules XREF_BIBR."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25036590
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Coimmunoprecipitation data demonstrated that overexpression of NLK protein reduced β-catenin binding to LEF-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 24395089
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Coimmunoprecipitation data demonstrated that overexpression of NLK protein reduced beta-catenin binding to LEF-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 24395089
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "As a result it has been further shown that β-catenin interacts with the transcription factor LEF1, which is in turn associated with Smad 4, thereby regulating the transcription of TGF-β target molecules xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25036590
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "While the full-length LEF1, transcribed from upstream promoter, interacts with beta-catenin and regulates Wnt target genes, the shorter isoform is incapable of binding beta-catenin and suppresses the regulation of Wnt targets through beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20843783
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Beta-catenin synthesized in vitro bound strongly and specifically to GST-LEF1 (XREF_FIG C, upper panel), but GAC63 synthesized in vitro did not (XREF_FIG C, lower panel)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17344318
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Nurr1 was reported to interact with β-catenin and Lef-1 in 293F cells [36] ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21362399
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This result indicates that β-catenin and Lef1 associate with chromatin at the Tcf/Lef element within the promoter region."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21689639
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "ChIP experiments demonstrated that β-catenin and Lef1 bind to the enhancer region of the gene."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21689639
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This regulation required Rac1 dependent phosphorylation of beta-catenin at specific serines, which when mutated (S191A and S605A) reduced beta-catenin binding to LEF-1 by up to 50%, as revealed by PLA and immunoprecipitation experiments."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "However, it is also possible that Rac1 stimulates beta-catenin nuclear transactivation function by stabilizing the interaction between beta-catenin and LEF-1 (summarized in XREF_FIG A)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Moreover, treatment with the Rac1 inhibitor NSC23766 resulted in a marked reduction in interactions between beta-catenin and LEF-1 in the nuclei of Wnt treated cells (XREF_FIG B)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "These results, when considered together, suggest that active Rac1 can stimulate complex formation between beta-catenin and LEF-1 and contributes to the positive effect of Wnt on the assembly of beta-catenin-LEF-1 complexes in cells (XREF_FIG B, D)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "To gain further insight into the effect of Rac1 on the interaction between beta-catenin and LEF-1 we analyzed the consequences of mutating the Rac1 sensitive beta-catenin serine residues S191 and S605 on binding of LEF-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Our findings instead suggest that Rac1 enhances the interaction between beta-catenin and LEF-1, providing a plausible new explanation for the role of Rac1 in augmenting transactivation of Wnt target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We propose that this stimulates binding of beta-catenin to LEF-1, leading to enhanced Wnt signaling responses."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Thus further understanding of the mechanism by which Rac1 modulates the interaction between LEF-1 and beta-catenin may provide an alternative therapeutic avenue to reduce beta-catenin-LEF-1 signaling in colorectal cancer."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Upon Wnt stimulation, LEF1 or other TCF/LEF family transcription factors associate with beta-catenin, a key cytoplasmic and nuclear mediator of Wnt pathway, and activate Wnt responsive target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21383983
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "β-catenin may increase FoxO transcriptional activity and competitively limit β-catenin interaction with members of the lymphoid enhancer factor/T cell factor family [ xref ] and β-catenin also has been demonstrated to be necessary for protection against amyloid toxicity in neuronal cells [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 18554157
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Moreover, binding of Lef1 and beta-catenin to the Lef1 binding site transactivates COX-2 promoter activity."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17945188
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Moreover, binding of Lef1 and beta-catenin to the Lef1-binding site transactivates COX-2 promoter activity."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17945188
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Upon accumulation, β-catenin interacts with members of the lymphoid enhancer factor/T-cell factor (Lef-1/TCF) family to generate a functional transcription factor complex [2,6,7] which in turn induces constitutive transcriptional activation of distinct target genes, e.g. c-myc [8,9] ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20840866
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This regulation required Rac1-dependent phosphorylation of β-catenin at specific serines, which when mutated (S191A and S605A) reduced β-catenin binding to LEF-1 by up to 50%, as revealed by PLA and immunoprecipitation experiments."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "However, it is also possible that Rac1 stimulates β-catenin nuclear transactivation function by stabilizing the interaction between β-catenin and LEF-1 (summarized in xref A)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Similarly, the transient expression of WT-Rac1 or constitutively active (Q61L)-Rac1 caused a significant increase in nuclear β-catenin–LEF-1 interactions relative to control ( xref B)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Importantly, we were able to show by IP that the Rac1 inhibitor reduced formation of ectopic LEF-1–β-catenin complexes both before and after LiCl treatment ( xref C;–)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "These results, when considered together, suggest that active Rac1 can stimulate complex formation between β-catenin and LEF-1 and contributes to the positive effect of Wnt on the assembly of β-catenin–LEF-1 complexes in cells ( xref B,D)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "To gain further insight into the effect of Rac1 on the interaction between β-catenin and LEF-1 we analyzed the consequences of mutating the Rac1-sensitive β-catenin serine residues S191 and S605 on binding of LEF-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We next used immunoprecipitation assays to measure binding efficacy between myc-tagged forms of β-catenin and LEF-1, using an anti-HA antibody to pull down ectopic HA-LEF-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Our findings instead suggest that Rac1 enhances the interaction between β-catenin and LEF-1, providing a plausible new explanation for the role of Rac1 in augmenting transactivation of Wnt target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We propose that this stimulates binding of β-catenin to LEF-1, leading to enhanced Wnt signaling responses."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Our model ( xref ) details the proposed steps for Rac1 involvement in canonical Wnt signaling: (1) activation (GTP-loading) of Rac1 reduces its association with the plasma membrane and causes active Rac1–β-catenin complexes to dissociate from the membrane, (2) these active Rac1–β-catenin complexes translocate to the cytoplasm and nucleus, (3) active Rac1 mediates the phosphorylation of β-catenin at S191 and S605 via JNK2 kinase in the cytoplasm and possibly the nucleus, and (4) the newly phosphorylated β-catenin preferentially binds to LEF-1 to boost gene transactivation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Chibby and ICAT, two physiological inhibitors of β-catenin–LEF-1 interaction ( xref ; xref ) both bind within this region, whereas LEF-1 binds between S191 and S605 within the arm repeats 3–9 ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We speculate that addition of the phosphate group at S605 might disturb interaction between β-catenin and the inhibitors, but retains or enhances β-catenin interaction with LEF-1 and recruitment of transcriptional co-activators such as BCL9, pygopus, Mediator and CBP ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The development of small molecule inhibitors targeting β-catenin–LEF-1 interactions have been met with specificity difficulties due to an overlapping binding region between LEF-1/TCFs and destruction complex members such as APC and Axin ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Thus further understanding of the mechanism by which Rac1 modulates the interaction between LEF-1 and β-catenin may provide an alternative therapeutic avenue to reduce β-catenin–LEF-1 signaling in colorectal cancer."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The thermodynamics of beta-catenin binding to E-cadherin, Lef-1, APC, axin, and the transcriptional inhibitor ICAT have been determined by isothermal titration calorimetry."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 16293619
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Nuclear CTNNB1 binds the transcription factors LEF1 (lymphoid enhancer binding factor 1) and TCF (T-cell factor), thereby converting LEF1 into a transcriptional activator."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23442321
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In agreement with these results, several of the new candidates bind to LEF1, and it is well established that TCF and LEF1 forms a complex with beta-catenin to activate the expression of genes under the canonical Wnt pathway [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20515496
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Being expressed in the nucleus, β-catenin could bind with lymphoid enhancing binding factor-1/T-cell factor-4 (LEF-1/TCF-4) xref , xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26078714
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Beta-catenin and LEF1 complexes formed in the absence of KLF4 (XREF_SUPPLEMENTARY), and, interestingly, TCF4 and KLF4 complex formation was unaffected by beta-catenin deletion (XREF_SUPPLEMENTARY)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28589954
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Importantly, LEF-1-bound beta-catenin was protected from degradation by APC and axin in SW480 colon cancer cells."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 11986304
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Lef-1 and β-catenin form a functional transcription factor complex as a result of Wnt signaling, and their overexpression can bypass a Wnt signal ( Behrens et al., 1996 )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12039047
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This could be explained by a likely association of over-expressed Lef-1 protein with endogenous β-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12039047
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Accumulated β-catenin associates with members of the lymphoid enhancer-binding factor/T-cell factor (LEF/TCF) family to regulate specific target genes such as c-myc, cyclin D1, CD44, and Axin2 xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27668402
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Central regions of Tcf7s and Lef1 interact weakly with β-catenin and strongly with co-repressors, including Hdacs and Tle proteins."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22079544
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Further, while it is known that beta-catenin and LEF1 are transcriptional coactivators and form a complex within the nucleus, and that beta-catenin co-activates LEF1 transcription, these proteins are rarely studied together in context of disease."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27067790
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Furthermore, mammalian Dact1 inhibits the binding of Lef-1 with beta-catenin and promotes the interaction of Lef-1 with histone deacetylase (HDAC1) a co-repressor XREF_BIBR, XREF_BIBR."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23349981
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Dapper1, originally identified as a protein binding to Dishevelled, can associate with HDAC1 and enhance the interaction of Lef1 with HDAC1 (at the expense of Lef1 binding to beta-catenin), thus maintaining Lef1 in the repressive state, as previously proposed."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19433865
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Furthermore, mammalian Dact1 inhibits the binding of Lef-1 with β-catenin and promotes the interaction of Lef-1 with histone deacetylase (HDAC1) a co-repressor xref , xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23349981
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "It was previously shown that Dact2 inhibits Lef-1 interaction with β-catenin, however, Dact2 does not appear to inhibit the interaction of Pitx2 with β-catenin or Lef-1, but modulates their synergistic transcriptional activities."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23349981
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the nucleus, beta-catenin binds to and activates the TCF/LEF transcription factor complex to induce the expression of wnt-pathway genes, such as, EphB2, EphB3, TCF4 and LEF1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19956666
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the nucleus, beta-catenin interacts with the transcription factor LEF-1, which activates transcription of the target genes [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22229032
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Based on these insights, new therapeutic opportunities have been explored by investigators who show that small-molecule WNT-pathway inhibitors, which inhibit the interaction between β-catenin and LEF1, selectively induce cell death in AML cell lines and primary AML blasts [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27571104
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Based on these insights, new therapeutic opportunities have been explored by investigators who show that small-molecule WNT-pathway inhibitors, which inhibit the interaction between beta-catenin and LEF1, selectively induce cell death in AML cell lines and primary AML blasts [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27571104
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The chromatin immunoprecipitation assay assay showed beta-catenin and LEF -1 heterodimer could directly bind to the promoter of Mre11 in vivo."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20956948
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Expression of the MYF5 gene is regulated directly by CTNNB1 and LEF1 binding and transcriptional co-activation of the MYF5 epaxial enhancer XREF_BIBR."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28165492
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Subsequently beta-catenin interacts with members of the lymphoid enhancer factor 1/T-cell factor (LEF1/TCF) family, resulting in a functional transcription factor complex and the expression of downstream target genes. xref , xref The non-canonical Wnt ligands also bind to FZD family receptors, and ROR2 and RYK co-receptors. xref - xref The non-canonical signaling pathways include three pathways (Wnt/Ca 2+ , Wnt/G protein, and Wnt/PCP signaling pathways) and non-canonical signaling regulates cell polarity and movement. xref - xref Among the five Wnt antagonist families (secreted frizzled-related protein (sFRP), Wnt inhibitory factor 1 (Wif1), Xenopus Cerberus, Wise and Dickkopf (DKK) families) the DKK family consists of four main members (DKK1-4) which contain two distinct cysteine-rich domains. xref , xref Our lab has studied several Wnt antagonist genes and these function in renal cancer. xref - xref Previously DKK4 has been thought to act as an inhibitor of Wnt/beta-catenin signaling in colorectal cancer. xref , xref Recently two groups found that DKK4 expression was increased in colon cancer tissues compared to matched normal colon tissues and DKK4 was induced by activated beta-catenin, although DKK4 itself significantly inhibited TCF/LEF reporter activity in colon cancer cell lines. xref , xref As far as we know, there have been no reports regarding DKK4 and renal cancer."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21472712
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Wnt signaling induces the stabilization of cytosolic beta-catenin, which then associates with lymphoid enhancer factor and T-cell factor (LEF-1/TCF) to form a transcription complex that activates Wnt target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12556497
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Subsequent transforming growth factor (TGF)-beta3 signaling increases LEF-1 gene expression causing formation of beta-catenin-lymphoid enhancer factor (LEF)-1 complexes that initiate EMT."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 18799618
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "It is known that nuclear beta-catenin is able to bind TCF and Lef1 and activate transcription XREF_BIBR, XREF_BIBR."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25003522
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "To determine whether the beta, catenin, LEF1, and TCF complex might directly regulate Cacna1g, Cacna2d2, Kcna6, Kcnh8, Drd3, Gabra3, Glra1, Grid2, and Calb2, we analyzed the in vivo binding of beta-catenin to LEF1 and TCF motifs within the CNSs using a chromatin immunoprecipitation (ChIP) assay with designed primers."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23157480
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Here, the stable β-catenin interacts with the lymphoid enhancer factor/T cell factor (LEF/TCF) and is translocated into the nucleus as a complex of β-catenin/LEF/TCF to stimulate target gene transcription by displacement of groucho-HDAC co-repressors xref , xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23490077
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Beta-catenin and LEF1 signaling is activated in CLL cells, and inhibition of beta-catenin and LEF1 interaction has been proved to induce apoptosis in CLL cells [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26950276
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "However, our results also demonstrate that a complete loss of E-cadherin and transformation to the mesenchymal phenotype are dependent on Smad signaling, which subsequently stimulates formation of beta-catenin and LEF -1 complexes that induce EMT."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 16467384
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The mutations impaired both LEF1 binding to beta-catenin and transcriptional activation, and are the first tumor-associated mutations that inactivate Wnt signaling."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 16565724
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "As transcriptional activity of Lef1 is controlled by recruitment of its co-activator β-catenin, we tested whether Lef1 and β-catenin can form a ternary complex on the TCF/LEF site ( xref F and 5G)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26212321
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The mutations impaired both LEF1 binding to beta-catenin and transcriptional activation, and are the first tumor associated mutations that inactivate Wnt signaling."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 16565724
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The same is true for mammalian cells, where FHL2 does not interfere with the β-catenin–LEF-1 interaction (unpublished data)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12370240
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This TNKSi insensitivity is conferred by β-catenin’s association with LEF1 and BCL9-2/B9L, which accumulate during Wnt stimulation, thereby providing a feed-forward loop that converts transient into chronic β-catenin signaling."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 24419084
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Intratumoral, peritumoral and Crohn’s-like lymphoid reactions were not significantly associated with nuclear CTNNB1 expression ( P > 0.14)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29510987
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the nucleus, β-catenin associates with the lymphoid enhancer factor/T-cell factor (LEF/TCF) family of transcription factors, converting them from transcription repressors to activators, thereby activating a wide range of Wnt responsive genes xref – xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31273221
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This correlated with upregulation of LEF1, a nuclear binding partner of beta-Catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31463973
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Both, complete LEF1-deficiency and prevention of the β-Catenin-LEF1 interaction impaired the ability of SNAIL1 to elicit expression of an alternative set of Wnt/β-catenin targets, and to promote cancer cell invasion."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31463973
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "To directly assay for binding of LEF-1 and beta-catenin to these sites, electrophoretic gel-mobility shift analysis was performed with recombinant LEF-1 and beta-catenin produced in E. coli."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10704849
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "These experiments show that there is specific binding of the beta-catenin and LEF -1 complex to either or both of the two TCF recognition sites in the Brachyury promoter."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10704849
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Similarly, the interaction between nuclear beta-catenin and TCF-4 or LEF-1 was reduced by CaSR activation, indicating an inhibitory association of beta-catenin and TCF-4 or LEF-1 in the nucleus."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27108064
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "LEF-1 and beta-catenin form a complex that regulates promoter activation in concert with PAX-5 in immature B cells (Jin et al., 2002)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21184762
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Because LEF-1 possesses its own NLS (Huber et al., 1996) and is able to interact with beta-catenin, we asked whether glucose favored LEF-1 and beta-catenin interaction as a possible mechanism for the nuclear accumulation of beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Thus, the availability of LEF-1 may be critical for LEF-1 and beta-catenin interaction and nuclear accumulation.Given that acetylation of beta-catenin through p300 or CBP (Wolf et al., 2002; Xu and Kimelman, 2007) and its deacetylation by SIRT1 (Firestein et al., 2008) impacts its oncogenicity, we examined whether combined LiCl and glucose treatment altered the levels of the acetylase p300 and the deacetylase SIRT1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Collectively, the results indicate that combined glucose and LiCl treatment increases p300-LEF-1-beta-catenin interaction while preventing SIRT1-LEF-1 and SIRT1-beta-catenin interaction and suggest that increased acetylation by p300 may be required for nuclear retention of the LEF-1 and beta-catenin complex.Endogenous beta-catenin underwent nuclear accumulation following glucose and LiCl stimulation; by contrast, a beta catenin mutant unable to interact with LEF-1 (Delta19 beta-catenin) (Orsulic and Peifer, 1996; Prieve and Waterman, 1999) was unable to accumulate in the nucleus, suggesting that beta-catenin and LEF -1 interaction was required for nuclear retention.The requirement of LEF-1 for mediating the p300 and beta-catenin interaction was examined in a western blot for p300 on anti-myc immunoprecipitates from cells transfected with Myc tagged wild-type (WT) or Delta19beta catenin mutant and treated with LiCl, glucose, both, or none."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Whereas ectoptic WT beta-catenin bound p300 upon exposure of the cells to both glucose and LiCl (like endogenous beta-catenin), conditions that promote LEF-1 and beta-catenin interaction and nuclear accumulation of beta-catenin, the Delta19 beta catenin mutant was unable to bind p300 under any conditions."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Given that p300 binds the C terminus of beta-catenin (Hecht et al., 2000) more than 300 amino acids away from the region missing in Delta19 beta-catenin (arms 5-6), our results suggest that LEF-1 and beta-catenin complexes recruit p300 more efficiently than does beta-catenin alone."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Thus, the LEF-1 and beta-catenin interaction promoted by the combination of glucose and LiCl appears critical for stabilizing p300 interaction and nuclear retention.To test whether beta-catenin acetylation is increased under the conditions required for nuclear accumulation, we immunoprecipitated nuclear extracts with acetyllysine antibody and detected beta-catenin or immunoprecipitated with anti-beta-catenin and detected anti-acetyllysine."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Although the precise molecular mechanisms have not been explored here, we view it as probable that SIRT1 acts as a competitor for interaction between LEF-1 and beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Treatment with lithium chloride, microinjection of constitutive beta-catenin, HDAC1 IgG, or the addition of serum all acted to reverse this repressive function.Immunoprecipitation from cells transfected with a Pitx2 expression vector revealed the ability of Pitx2 to robustly interact with beta-catenin and with HDAC1, even at endogenous levels of Pitx2 and beta-catenin, analogous to interactions between TCF and LEF1 and beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12464179
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This data implicates a strong association between beta-catenin and LEF-1 within the nuclei of cells, as confirmed by immunoprecipitation assay with the nuclear chromatin fraction of MCF-7 cells."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26844628
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "How does the binding of beta-catenin to LEF-1 and TCF proteins affect cooperative interactions at a promoter or enhancer?"
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10209158
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "LiCl activation of the Wnt and beta-catenin signaling pathway causes nuclear accumulation of beta-catenin, which binds with TCF and LEF1 transcription factor to modulate the expression of downstream genes such as C-myc and cyclin D1 that regulate the cell cycle and growth (Kanei-Ishii et al., 2004)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29679546
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In human osteoblasts, a naturally occurring LEF1 isoform lacking the N-terminus can still associate with beta-catenin and activate Wnt targets (Hoeppner et al."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22305157
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In addition, an antisense DeltaNp63alpha construct (DeltaNp63alpha-AS) reduced luciferase activity mediated by the endogenous beta-catenin and Lef -1 complex, while TAp63alpha had no effect on this activity."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12086851
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the nucleus beta-catenin binds to Lef1 and TCF transcription factors to initiate gene activation.The importance of Wnt signaling in bone development became obvious following the discovery of gain- and loss-of-function mutations of human LRP5."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19389391
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Further, Shtutman and colleagues 83 demonstrated that the beta-catenin and LEF -1 complex binds to the cyclin D1 promoter, and the promotion of cyclin D1 expression by beta-catenin is inhibited by transfection with wild-type APC and/or AXIN."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 11839557
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "93 The changes in COX-2 described in the latter study model are also associated with increased beta-catenin and LEF -1 complex formation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 11839557
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "LEF-1 and TCF proteins associate with beta-catenin through amino-terminal sequences, and together these proteins mediate a transcriptional response to Wnt signaling.Wnt proteins represent a growing family of secreted signaling molecules that are expressed in diverse tissues and have been shown to influence multiple processes in vertebrate and invertebrate development (reviewed in Cadigan and Nusse 1997)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10933391
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "LEF1, in turn, binds to the promoter regions of at least two direct target genes (Lmx1a and Fgf20) and together with beta-catenin appears to activate their transcription in the mutant VM."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26049140
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "However, in-depth study is needed to investigate the mechanisms regulating GSK3beta activity in PDLSCs under inflammatory conditions.Our findings demonstrated that in the inflammatory microenvironments, TNF-alpha induced the phosphorylation of GSK3beta, and the p-GSK3beta subsequently resulted in nuclear beta-catenin accumulation and beta-catenin and Lef -1 complex formation, which inhibited Runx2 associated the osteogenesis of PDLSCs."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23911749
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "29 These inhibitors and their mechanism of action (inhibition of LEF-1 and beta-catenin binding) are being more fully explored with the development of a LEF-1 and beta-catenin FRET assay."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12659745
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In pull-down assays, HINT1 did not bind to beta-catenin or LEF-1 nor did it impair the LEF-1 and beta-catenin interaction or the binding of pontin or reptin to beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21316334
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This increases the pool of cytoplasmic beta-Catenin, which translocates to the nucleus and then binds with LEF-1 to modulate transcription of target genes.A role for the TGF-beta pathway in desmoid cells can be hypothesized based on its central role in fibroblastic proliferation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17601559
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "T246M) mutant does not affect the interaction, while knockdown of CHIP decreases the endogenous interaction of LEF1-β-catenin complex."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33431799
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Moreover, the miR-371-373 cluster is activated by Wnt signaling through the direct binding of beta-catenin and LEF1 to the promoter region and then forms a positive feedback loop with Wnt signaling by repressing DKK1 [25]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26255203
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Functional interaction of beta- catenin with the transcription factor LEF-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10503270
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The nuclear beta-catenin interacts with the transcription factor LEF1 to activate target gene expression (Behrens et al., 1996; Hsu et al., 1998)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 16890162
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "These results suggest that Tob1a inhibits the interaction between beta-catenin and LEF1 solely by competing with LEF1 for beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 16890162
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Thus, expression of the OSTM1DeltaC mutant blocked the ability of Wnt3a to signal to a major embryonic response, PE formation.The epistasis experiments implicated OSTM1DeltaC action on Wnt and beta-catenin signaling, downstream of beta-catenin accumulation, but upstream of beta-catenin and Lef1 interaction."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 18296023
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We compared the ability of OSTM1 and OSTM1DeltaC to regulate the Wnt3a induced binding of beta-catenin to Lef1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 18296023
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Beta-catenin binding to Lef1 increased in cells treated with Wnt3a."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 18296023
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Furthermore, we determined the mechanism whereby OSTM1 regulates Wnt signaling : through modulation of the beta-catenin and Lef1 interaction, a crucial downstream event in Wnt and beta-catenin signal transduction.In order to assess the effect of OSTM1 overexpression or suppression on Wnt and beta-catenin signaling we utilized a luciferase based Lef and Tcf sensitive reporter construct which can be activated in response to Wnt3a or transfection with constitutively active Wnt signaling components [21]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 18296023
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Therefore, we tested the ability of OSTM1 and OSTM1DeltaC to regulate the beta-catenin and Lef1 interaction."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 18296023
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "It will be informative to probe the beta-catenin and Lef1 interaction in cells from grey-lethal mice or ARO patients with OSTM1 deficiency.Finally, expression of OSTM1DeltaC was shown to inhibit the formation of primitive endoderm in response to Wnt3a."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 18296023
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Quantitative analysis indicated that SPDEF disrupted the binding between beta-catenin and TCF1 or TCF3, but did not affect the binding between beta-catenin and LEF1 or TCF4."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28390865
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Stimulation of mutated Wnt and beta-catenin pathway is well recognized as an early stage in colon carcinogenesis that eventually leads to transcription of beta-catenin and LEF -1 complex such as c-Myc and cyclin-D1 [60]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26482805
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Similar mutations, leading to an increased half-life of beta-catenin, were identified in several human melanoma cell lines leading to a constitutively active beta-catenin and Lef -1 complex in these cells [91]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10528152
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The significance of this was poorly understood until yeast two hybrid studies revealed that the architectural HMG-box transcription factors LEF-1 and XTCF-3 interact with beta-catenin (Behrens et al., 1996; Molenaar et al., 1996)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10495268
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Upon accumulation, beta-catenin interacts with Lef-1 and Tcfs to generate a functional transcription factor complex (Behrens et al. 1996; van de Wetering et al. 1997)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9845363
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In addition, we found evidence that this effect is mediated by Cdx-1 in cells that give rise to the cervical vertebrae.Previous studies have found that cdx-1 expression is stimulated by Wnt signaling through Tcf binding sites in cultured cells, and further that LEF1 and beta-catenin complexes bind to a cdx-1 promoter fragment directly in vitro (Lickert et al., 2000; and M.I. and S.T., unpublished data)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 11335109
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "She found that the HMG box protein TCF-1 and Lef-1 can associate with beta -catenin, and can bind to target DNA as a complex [16]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9061012
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Elevated beta-catenin levels in colorectal cancer caused by mutations in either beta-catenin or in the adenomatous polyposis coli protein, which regulates the degradation of beta-catenin, result in the increased formation of transcriptionally active beta-catenin and LEF -1 complexes [82,83]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 11223032
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In both Xenopus and Drosophila, LEF-1 and TCF-1-like proteins bind beta-catenin and appear to translocate with it to the nucleus (Behrens et al. 1996; Riese et al. 1997; van de Wetering et al. 1997)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9288749
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Additionally, beta-catenin interacts with Lef-1 and with Klf4 to activate tert gene transcription."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25262238
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "These data suggest that Lef1 requires the HMG domain to activate the Col11a1 promoter, either through its DNA binding domain or through interaction with other proteins.In many systems, Lef1 interaction with beta-catenin is necessary for Lef1 mediated activation of target genes (reviewed in Behrens et al. 1996; Clevers and van de Wetering, 1997)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 18280717
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Behrens, J. et al. (1996) Functional interaction of beta-catenin with the transcription factor LEF-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27568239
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "During gastrulation, β-catenin forms a complex with LEF-1 to bind and inhibit the transcription of CDH1 and induce EMT [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32334405
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "During gastrulation, beta-catenin forms a complex with LEF-1 to bind and inhibit the transcription of CDH1 and induce EMT [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32334405
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In particular, the increased Histone Deacetylase 6 (HDAC6) expression induces the activation of the nuclear β-catenin-lymphoid enhancer binding factor 1 (LEF1)/T-cell factor-4 (TCF4) transcriptional complex and, in turn, that of the oncogene c-Myc [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32764471
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Cell (Cambridge, Mass.) 111, 251–263 *Behrens, J., von Kries, J.P., Kuhl, M., Bruhn, L., Wedlich, D., Grosschedl, R. and Birchmeier, W. (1996) Functional interaction of β-catenin with the transcription factor LEF-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 15715524
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Behrens J, von Kries JP, Kuhl M, Bruhn L, Wedlich D, Grosschedl R, Birchmeier W. Functional interaction of beta-catenin with the transcription factor LEF1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25914082
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Ets gene PEA3 cooperates with beta-catenin-Lef-1 and c-Jun in regulation of osteopontin transcription."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 18343725
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Functional interaction of beta-catenin with the transcription factor LEF-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22716317
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Cell 119, 641–651
+Behrens, J., von Kries, J. P., Kuhl, M., Bruhn, L., Wedlich, D., Grosschedl, R. and Birchmeier, W. (1996) Functional interaction of beta-catenin with the transcription factor LEF-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 18823282
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Here, β-catenin interacts with members of the lymphoid enhancer-binding factor (LEF)1/T-cell factor (TCF) transcription factor family and is translocated into the nucleus to stimulate transcription of a wide range of target genes, such as myc and cyclin D1, which are important regulators of epithelial cell proliferation [37,38]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 30743809
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Behrens J, von Kries JP, Ku¨ hl M, Bruhn L, Wedlich D, Grosschedl R et al. Functional interaction of beta-catenin with the transcription factor LEF-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22716317
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Of note, β-catenin binds to Lef1 and activates Lef1 transcription complex, leading to down-regulation of E -cadherin expression [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33388549
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Additionally, inhibiting the binding of LEF1 to beta-catenin impaired AML growth, in contrast to spared normal hematopoietic stem cells [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32872365
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The transient or stable expression of Wnt1 could induce the formation of β-catenin–LEF1 complex and the persistent activation of canonical Wnt signaling in CRC cells ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32923386
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the nucleus, β-catenin binds and activates the lymphoid enhancer factor (LEF)/T cell factor (TCF) transcription factor to regulate the expression of Wnt target genes ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33071819
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Behrens J, Von Kries JP, Kuhl M, Bruhn L, Wedlich D, Grosschedl R, et al. Functional interaction of β-catenin with the transcription factor LEF-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12975039
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Functional interaction of beta-catenin with the transcription factor LEF-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12975039
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Rac1 also enhances the nuclear formation of the β-catenin and LEF-1 complexes, providing the basis for the role of Rac1 in augmenting transactivation of Wnt target genes [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32178475
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The interactions between β-catenin and LEF1 have been historically viewed as inseparable."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32824435
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Binding of WNT ligands to their receptor stabilizes β-catenin (encoded by CTNNB1 ), which binds to the transcription factors of the lymphoid enhancer-binding factor (LEF) transcription factor (TCF) family."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21472002
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Optimizations of a novel fluorescence polarization based high-throughput screening assay for beta-catenin and LEF1 interaction inhibitors."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32956692
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "It is accomplished by transactivating MMP-13 promoter activity through LEF1 and beta-catenin binding to the LEF1 binding site in the 3 ' region of the MMP-13 genomic locus."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33572320
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Moreover, beta-catenin and LEF1 interaction regulates beta-catenin nuclear transport as well as the transcriptions of the key oncogenes in Wnt and beta-catenin signaling pathway."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32956692
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "To date, no economical and rapid high-throughput screening (HTS) assay has been reported for the discovery of beta-catenin and LEF1 interaction inhibitors."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32956692
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In this study, we developed a novel fluorescence polarization (FP)-based HTS assay to identify beta-catenin and LEF1 interaction inhibitors."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32956692
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "GST pull-down and surface plasmon resonance (SPR) assay demonstrated that beta-catenin and LEF1 interaction is a potential anticancer target of sanguinarine in vitro."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32956692
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This newly developed FP screening assay will be vital for the rapid discovery of novel Wnt inhibitors targeting beta-catenin and LEF1 interaction."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32956692
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "As expected, treatment with Wnt3A or EGF increased the binding of β-catenin to LEF-1 ( xref ), and depletion of LEF-1 ( xref ) reduced Wnt3A- or EGF-induced luciferase activity ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32860047
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Prompted by these findings, we investigated whether TG2 could affect the interaction of β-catenin with LEF1, inside the nucleus, necessary to induce Wnt genes expression."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33674551
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Elevated levels of DVL3 have also been reported in nonsmall cell lung carcinoma. xref CSNK2A2 (overexpressed 11-fold) is a highly conserved and ubiquitous serine/threonine kinase, over-expressed in many tumors including prostate. xref Cytoplasmic CSNK2A2 opposes the inhibitory role of GSK3B by phosphorylating and activating DVL and β-catenin, while nuclear CSNK2A2 enhances β-catenin:LEF1 transactivation of Wnt target genes. xref Its nuclear localization was previously reported as a poor prognostic indicator in prostate cancer, xref potentially through its promotion of AR-dependent transcription. xref Downstream component of the pathway, transcription factor LEF1 was also significantly over-expressed and has recently shown to promote AR expression and consequently enhance growth and invasion ability of prostate cancer cells in vitro . xref "
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22915211
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Nuclear β-catenin binds to and activates T cell factor (TCF) and lymphoid enhancer-binding (LEF) TFs, which directly bind to specific DNA sequences, enhancing the transcription of genes such as Cyclin D, c-Myc, and Axin2 and inhibiting transcription of other genes such as E-cadherin and the tumor suppressor p53 [ xref , xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33668092
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Considering the specific expression of Lef1 in the RG cells and not in the IPCs, the induction of β-catenin expression should form heterodimeric activators with Lef1 in the RG cells and lead to downstream target activation and generation of IPCs from the RG cells."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23257686
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Examination of individual NC genes confirmed the association of LEF1 and CTNNB1 with tissue-specific enhancers."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33852891
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We selected 30 genomic regions that interact with promoters of NC genes and are associated with both LEF1 and CTNNB1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33852891
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "To define the regulatory features of Wnt-associated elements, we examined how LEF1 and CTNNB1 interact with Axud1E1 , an enhancer directly activated by canonical Wnts ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33852891
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In our CUT&RUN datasets, approximately 37% of LEF1 binding and 43% of the LEF1/CTNNB1 binding takes place in cis-regulatory regions that only have suboptimal TCF/LEF motifs."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33852891
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "These results are consistent with a scenario in which the loss of Wnt activity in migratory cells drives the dissolution of the LEF1/CTNNB1 complex, leading to the subsequent silencing of the specification GRN."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33852891
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Lef1, a nuclear mediator of Wnt signaling, associates with beta-catenin and activates Wnt responsive genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33329029
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In addition, recent data demonstrates that at the onset of anagen, HG exchanging Tcf3/Tcf4 for Lef1 at the super-enhancers, and then Lef1 forms the nuclear complex with β-catenin after activation of WNT signaling (as a consequence of BMP inhibition) which drives HG committed progenitors ( xref ; xref ; xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33363148
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Lymphoid enhancer-binding factor 1 (Lef-1) and β-catenin bind the Pitx2 gene promotor in mice, indicating a role for the canonical Wnt signaling pathway in regulating Pitx2 expression."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33425902
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Of note, beta-catenin binds to Lef1 and activates Lef1 transcription complex, leading to down-regulation of E-cadherin expression [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33388549
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We can see this demonstrated using synthetic peptide BLBD, which can bind to β-catenin and LEF-1 sites, which results in the decreased formation of breast cancer cells [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
PMC
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We show that overexpression of CHIP promoted the activation of Wnt signaling, and enhanced the interaction between LEF1 and β-catenin through heightening the K63-linked polyubiquitin chains attached to LEF1, while the knockdown of CHIP had the opposite effect."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33431799
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Overexpression of CHIP increased the activation of Wnt signaling, and promoted β-catenin nucleus accumulation by enhancing the interaction between LEF1 and β-catenin through heightening the K63-linked ubiquitination of LEF1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33431799
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "As shown in Fig. xref , CHIP enhanced the interaction between β-catenin and LEF1, and the interaction was further heightened after Wnt3a treatment, while knockdown of CHIP decreased the interaction (Fig. xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33431799
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Moreover, overexpression of CHIP T246M mutant couldn’t affect the interaction of β-catenin-LEF1 complex (Fig. xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33431799
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "To illuminate whether the heightened interaction of β-catenin and LEF1 was owing to the function of Wnt3a treatment in Fig. xref , we detected the interaction between β-catenin and LEF1 as shown in Fig. xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33431799
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Under Wnt3a stimulation, the interaction between β-catenin and LEF1 was enhanced, and the interaction was more strengthened with the presence of CHIP."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33431799
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We further found CHIP increased the endogenous interaction between β-catenin and LEF1, while CHIP T246M hardly influenced the interaction (Fig. xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33431799
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Knockdown of CHIP reduced the endogenous interaction between β-catenin and LEF1 (Fig. xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33431799
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "These data suggested that CHIP enhanced the interaction between β-catenin and LEF1, and the interaction was further heightened with Wnt3a treatment."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33431799
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "It was in consistent with the enhanced interaction between β-catenin and LEF1 after Wnt3a treatment."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33431799
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Moreover, we verify that CHIP promotes the endogenous interaction between LEF1 and β-catenin, the CHIP (p."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33431799
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Taken together, these results indicate that CHIP accelerates β-catenin nucleus accumulation by promoting interaction between LEF1 and β-catenin through heightening the K63-linked ubiquitination of LEF1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33431799
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "AEG-1 forms a complex with LEF1 and β-catenin, and AEG-1-mediated activation of the Wnt/β-catenin pathway facilitated the maintenance of glioma stem-like cells and their self-renewal [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
PMC
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Intracellular signal transduction results in the stabilization and nuclear localization of β-catenin, which forms a complex with T cell transcription factors (TCFs) or lymphoid enhancer binding factor 1 (LEF1) to regulate the transcription of target genes [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
PMC
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The nuclear β-catenin could then specifically bind to lymphoid enhancer factor-1 (LEF-1) transcription factors to activate FUT8 transcription."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 30619732
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "β-catenin is associated with the lymphoid enhancer factor/T-cell factor family (TCF), converting them from transcription repressors to activators, thereby activating several Wnt-responsive genes ( xref – xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33574925
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In normal epithelial cells, the inhibitory effect of E-cadherin against tumorigenesis is maintained by sequestering the binding of β-catenin to lymphoid enhancer factor (LEF)/T cell factor (TCF); this process could impede the transcription of genes involved in the proliferation-associated Wnt signaling pathway xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33531990
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "And the depletion of FOXG1 diminished the binding of β-catenin to the LEF-1 promoter (Fig. xref e)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31771611
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the nucleus, beta-catenin directly interacts with LEF-1, activates the keratin gene, promotes hair growth and regulates the HF cycle."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33393222
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Disassembly of the destruction complex can block β-catenin degradation that results in the translocation of the accumulated β-catenin into the nucleus, where β-catenin binds to the lymphoid enhancer factor/T-cell factor (LEF/TCF) family of transcription factors and triggers the protooncogene-induced transcription of several target genes, such as c-myc, MDR1, OCT4, and cyclin D1 (refs. xref – xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33723262
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Intracellular signal transduction results in the stabilization and nuclear localization of β-catenin, which forms a complex with T cell transcription factors (TCFs) or lymphoid enhancer binding factor 1 (LEF1) to regulate the transcription of target genes [8]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
PMC
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Another study showed that SP5 and SP8 both assist the binding of β-catenin to LEF1 upon Wnt activation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 34552611
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We demonstrated that LEF1 and β-catenin bind to and regulate MIR17HG promoter activity, and that activation of the Wnt/β-catenin pathway increases the MIR17HG promoter activity as well as the endogenous expression of miR-17-92 cluster, whereas inactivation of the Wnt/β-catenin pathway reduces miR-17-92 cluster expression."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31191623
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Taken together, all these data suggest that LEF1 and β-catenin specifically bind to and regulate the MIR17HG promoter."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31191623
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "To validate whether LEF1 and β-catenin interact to regulate MIR17HG, we determined the effect of LEF1 and β-catenin interaction on MIR17HG promoter activity."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31191623
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Our results showed that LEF1 and β-catenin could bind to and regulate the MIR17HG promoter (Figure 2)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31191623
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "And SNAIL affects the EMT process of CRC through various mechanisms such as β-Catenin-LEF1 complexes [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
PMC
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the nucleus, CTNNB1 forms a complex with LEF1, inhibits the transcriptional activity of E-cadherin, and induces epithelial-mesenchymal transition [23]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31511060
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "SOX9 has been shown to interact with β-catenin [ xref ], which in turn binds to LEF1 [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 35191090
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "SOX9 physically interacts with β-catenin [ xref ], which in turn binds to LEF1 [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 35191090
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "By qPCR analysis, RUNX2, ALP, and SPARC, which are associated with osteogenic differentiation, were significantly increased 2.47 ± 0.73-fold ( p = 0.011), 2.57 ± 0.09-fold ( p = 0.004), and 2.13 ± 0.60-fold ( p = 0.018), respectively; LEF1 and β-catenin, which are associated with the Wnt/β-catenin pathway, were increased 1.89 ± 0.45-fold ( p = 0.012) and 2.22 ± 0.51-fold ( p = 0.017), respectively (Fig. xref a)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31753039
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Further, a more recent study suggests that Wnt-mediated Rac1 activation not only induces nuclear translocation, but also enhances β-catenin and LEF-1 binding in the nucleus ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 36248967
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the absence of E-cadherin, there was more interaction between β-catenin and LEF-1 than in E-cadherin wild-type cells."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 36248967
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "EpICD directly interacts with beta-catenin and lymphoid enhancer factor (LEF) [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31547062
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "However, this increase in NRARP:LEF1 interactions only translated into increased Wnt signaling activity in Loucy cells as determined by the increase in LEF1:β-catenin interactions in these cells upon NRARP overexpression (Fig. xref g and Supplementary Fig. xref , xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31586130
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In addition, our data shows that NRARP overexpression increases the number of NRARP:LEF1 interactions which in T-ALL cells with low levels of NICD1 translates into more LEF1:β-catenin interactions."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31586130
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Inside the nucleus, β-catenin interacts with T cell transcription factor (TCF) and lymphoid enhancer factor (LEF) and induces transcription of β-catenin target genes [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33049989
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Nuclear DACT2 disrupts the interaction between β-catenin and LEF1, and cytoplasmic DACT2 represses β-catenin nuclear transport via restoring E-cadherin–β-catenin complex [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32961708
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the nucleus, β-catenin binds to the lymphoid enhancer factor/T-cell factor to initiate the transcription of target genes that promote the proliferation, migration, and invasion of cancer cells [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32898953
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Furthermore, while the Wnt pathway does not initiate activation, the signaling components, β-catenin, and Lef1 , bind to the Pitx2 promotor and are subsequently required for maintaining Pitx2 expression."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33182738
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Indeed, functional activation of β-catenin-LEF1 was confirmed in the nail matrix just above the RSPO4 expressing mesenchyme (Fig. xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 34099859
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the activated Wnt signaling pathway, LEF1 forms dimers with over-aggregated β-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 36199361
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "YAP downregulation has been shown to sensitize BRAFi-resistant melanoma cells to MAPK pathway inhibitors, and YAP and Wnt signaling (via β-catenin-LEF1) co-regulate apoptosis of acquired MAPK inhibitor-resistant melanoma ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 34462276
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We showed that AEG-1 forms a complex with LEF1 and β-catenin, and AEG-1-mediated activation of Wnt/β-catenin pathway facilitated maintenance of glioma stem-like cells and their self-renewal ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 34353442
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The transient expression of Wnt1 induces the formation of β-catenin–LEF1 complex in nucleus, resulting in persistent activation of canonical Wnt/β-catenin signaling xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 36056132
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the nucleus, β-Catenin binds to T cell factor lymphoid enhancer factor family to activate downstream genes, such as cyclin D1 and C-myc, leading to inhibition of the expression of PPARγ and CCAAT/enhancer binding protein-α (C/EBPα) ( xref ; xref ; xref ; xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 36188620
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Lef1 interacts with β-catenin to activate the transcription of the Wnt target genes [ xref , xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 36140843
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "β-catenin which is then subsequently translocate to the nucleus interacts with T-cell factor and lymphoid enhancer factor (TCF/LEF)-family transcription factors ( xref ; xref ), initiating transcription of a set of pro-inflammatory, proliferative genes and oncogenes, such as CCND1 and MYC ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 34869313
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The effect of CGP049090 and PFK115-584, two compounds that specifically inhibit the binding between β-catenin and LEF1, was evaluated in Kasumi-1 and HL-60 AML cell lines, primary AML blasts and healthy peripheral blood mononuclear cells as a control."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31703382
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Wnt signaling promotes EC proliferation (Masckauchan et al., 2005, 2006) and the Lef1/Ctnnb1 complex induces cell cycle progression through transcriptional activation of Cyclin D1 (Shtutman et al., 1999)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19154719
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "To quantitatively measure the ability of GATA4 to block interaction between β-catenin and LEF1/TCF1, we turned to bimolecular fluorescence complementation assay 34 by fusing β-catenin and LEF1/TCF1 to N- and C-terminal half of firefly luciferase respectively, such that when these fusion proteins were expressed in HepG2 cells, luciferase activity is a direct readout for interaction between β-catenin and LEF1/TCF1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31903133
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Finally, β-catenin binds to TCF/LEF1 to replace the transcriptional suppressor Groucho in the target gene promoter, thereby regulating the expression of downstream target genes (c-Myc, Cyclin D1, etc.)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32038289
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Nuclear DACT2 disrupts the interaction between β-catenin and LEF1, and cytoplasmic DACT2 represses β-catenin nuclear transport via restoring E-cadherin–β-catenin complex [80]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32961708
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Chibby, a newly identified β-catenin antagonist, can disrupt the association between β-catenin and LEF1, and promotes β-catenin nuclear export via a chibby-14-3-3-β-catenin complex [81,82]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32961708
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "When stimulated with Wnt or genetic mutations of Wnt components, β-catenin accumulates in the cytoplasm, translocates into the nucleus and binds to LEF-1/TCF4 and some other coregulators, leading to the expression of target genes, such as c-Myc and MMP7."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33893396
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the context of cancer stemness, interactions between β-catenin and Lef1 transactivate the miR-371–373 cluster that mediates CSC self-renewal [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33817661
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "LEF1 binds to β-catenin to regulate the expression of downstream molecules, thereby regulating the signal pathway (42)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 34485109
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "However, there are clear discrepancies with the reported nuclear localisation of both β-catenin and the key canonical transcription factor lymphoid enhancer binding factor 1 (LEF1) in the pre-cortex [21,22] and the DP [20,23]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29738529
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "SNAIL1 is a transcription factor usually expressed during EMT; its expression has been demonstrated to induces EMT in gastric cancer [ xref ] although it may direct the cells towards a low proliferating phenotype trough the β-catenin-LEF1 complex [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31906104
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Finally, crystal structure of β-catenin ARM repeats 2–10 with a bound part of Lef1 β-catenin-binding domain (β-catenin-BD) revealed that Lef1 interacts with β-catenin in an analogous manner as other members of TCF family."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32486423
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The bioinformatic analysis revealed an array of putative nuclear factor-binding sites, including two potential LEF1/TCF like elements located on -349 bp – -333 bp and +19 bp – +35 bp (Figure 2A), on which β-catenin acts as co-activator of TCF transcription factors to regulate the transcription of target genes.To determine whether β-catenin binds to the LEF1/TCF elements of PME-1 promoter, we performed the ChIP assay in SH-SY5Y cells."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31714894
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We investigated whether inhibition of GSK-3β affects the binding of β-catenin with LEF1/TCF cis-elements."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31714894
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We performed ChIP assay and found that ARA014418 treatment increased the levels of co-immunoprecipitated cis-elements of LEF1/TCF-1 and LEF1/TCF-2 by β-catenin (Figure 7A), suggesting inhibition of GSK-3β enhances the binding of β-catenin to LEF1/TCF elements.To determine the effect of ARA014418 on PME-1 expression in primary cultured cortical neurons, we treated the neurons with various concentrations of ARA014418 for 4.5 hr and analyzed mRNA level of PME-1 by qPCR."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31714894
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We found that the promoter of human PME-1 contains two LEF1/TCF cis-elements, which act as enhancers to promote the expression of PME-1 and that binding of β-catenin to LEF1/TCF
+cis-elements enhances the expression of PME-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31714894
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The interaction between beta-catenin, lymphoid enhancer-binding factor 1 (LEF1), and transcription factor 4 (TCF4) was studied in order to see its effects on melanoma."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31934531
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "It was observed that the increase in TCF4 and decrease in LEF1 was associated with an invasive transformation of melanoma, in contrast with epithelial tumors where the upregulation of beta-catenin interaction factor LEF1 was seen to promote EMT [89, 90]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31934531
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In sphere cultures, we further observed that ectopic activation of WNT signaling using a β-catenin-LEF1 fusion protein xref (Fig. xref ) triggered a strong induction of NOTCH signaling (Fig. xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32066735
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Moreover, PKF115-584 (that disrupts the interactions between β-catenin and LEF1, leading to transcriptional inactivation of LEF1) prevented and partially reverted leukemogenesis, by inducing apoptosis and reducing proliferation in human T-ALL cells [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32046053
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This differential expression of the LEF isoform in LSC versus normal HSCs offers the opportunity to preferentially target LSCs by impairing LEF1-β-catenin interaction, and targeting Wnt/β-catenin axis."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32046053
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Moreover, PKF115-584 (that disrupts the interactions between β-catenin and LEF1, leading to transcriptional inactivation of LEF1) prevented and partially reverted leukemogenesis, by inducing apoptosis and reducing proliferation in human T-ALL cells [81]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32046053
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "DACT2 has been reported to decrease LEF1-β-catenin binding in colon cancer by competing with β-catenin [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 30359298
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Nuclear RAC1 in complex with β-catenin facilitates RAC1-dependent phosphorylation of β-catenin on serines 161 and 605, resulting in the formation of nuclear β-catenin-LEF-1, which is necessary for the transactivation of Wnt-dependent genes [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 35013128
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Finally, based on the fact that certain vertebrate TCFs encode either repressor or activator function and that repressive TCF proteins have higher affinities for Gro/TLE, a model has been proposed by which Gro/TLE in complex with repressive TCFs (e.g., TCF3) is released upon Wnt signaling, allowing for the recruitment of activating TCFs (e.g., LEF1) bound to β-catenin [18]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32759724
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the classical Wnt/β-catenin pathway, β-catenin accumulates in the nucleus and binds to TCF1/LEF1 to promote downstream target gene expression."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31060551
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The Lef1 distal region is bound by β-catenin in WNT-stimulated ESCs but not in normal ESC culture conditions [ xref E, compare β-catenin (WNTa) versus β-catenin (control); data from xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 36102628
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Nuclear beta-catenin and LEF1 were significantly associated with CTNNB1 mutation, showing nuclear beta-catenin a better specificity and positive predictive value for CTNNB1 mutation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 34420090
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "When LEF1 binds to beta-catenin, it activates epithelial-to-mesenchymal transition and cell proliferation [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 34420090
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In response to Wnt signaling, LEF-1 bound to both DNA and β-catenin activates Wnt-responsive genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 35177069
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the nucleus, beta-catenin interaction with the transcriptional effectors TCF and LEF1 converts the latter from transcriptional repressors to activators."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 24117889
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Dkk1 is thought to act as a soluble inhibitor for Wnt signaling [XREF_BIBR], while transcription factor Lef1 mediates Wnt signaling pathway by binding with its co-activator beta-catenin [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29121993
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "On the other hand, beta-catenin present in the nucleus binds to Lef1 (k 49) to increase the expression of mRNA of Delta gene (k 54), followed by translation of Delta (k 51) and translocated to the cytoplasm (k 58)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28106087
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "As a co-transactivator, β-catenin associates with the lymphoid enhancer factor (LEF)/T-cell factor (TCF) family of transcription factors and mediates expression of target genes involved in differentiation, polarization and cell fate xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23418486
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Co-immunoprecipitation results indicated that DACT2 strongly associated β-catenin as well as lymphoid enhancer-binding factor 1 (LEF1) and directly disrupted the formation of the β-catenin-LEF1 complex in the nucleus."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25023701
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Activation of the Wnt and beta-catenin pathway is characterized by 1) stabilization of cytoplasmic beta-catenin after receptor engagement by Wnt ligands; 2) beta-catenin nuclear translocation, 3) beta-catenin interaction with lymphoid enhancer binding factor-1 and T-cell factor-1 (LEF and TCF) transcription factors; and 4) stimulation of target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28838333
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "DACT2 was reported to promote Dvl degradation in a lysosome dependent pathway, and inhibits LEF1 binding to beta-catenin [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22806826
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The potential explanation is that DACT2 promotes Dvl degradation in a lysosome dependent pathway and inhibits LEF1 binding to beta-catenin [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22806826
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "DACT2 was reported to promote Dvl degradation in a lysosome-dependent pathway, and inhibits LEF1 binding to β-catenin [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22806826
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The potential explanation is that DACT2 promotes Dvl degradation in a lysosome-dependent pathway and inhibits LEF1 binding to β-catenin [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22806826
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "They antagonize the Wnt pathway by decreasing the cellular beta-catenin, disrupting the beta-catenin and LEF -1 complexes, and repressing the Wnt target genes [XREF_BIBR, XREF_BIBR, XREF_BIBR - XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23213540
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Coimmunoprecipitation showed a selective disruption of beta-catenin and LEF -1 interaction."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23213540
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The cytoplasmic tails of N- or E-cadherin colocalized with beta-catenin in the nucleus, and suppressed the constitutive LEF-1-mediated transactivation, by blocking beta-catenin-LEF-1 interaction."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9860970
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Interactions between beta-catenin and LEF-1 and TCF, APC and conductin and axin are essential for wnt controlled stabilization of beta-catenin and transcriptional activation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10966653
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Activation of the Wnt/β-catenin pathway is characterized by 1) stabilization of cytoplasmic β-catenin after receptor engagement by Wnt ligands; 2) β-catenin nuclear translocation, 3) β-catenin interaction with lymphoid enhancer-binding factor-1/T-cell factor-1 (LEF/TCF) transcription factors; and 4) stimulation of target genes ( xref – xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28838333
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The nuclear β-catenin interacts with the transcription factor LEF1 to activate target gene expression ()."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 16890162
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "These results suggest that Tob1a inhibits the interaction between β-catenin and LEF1 solely by competing with LEF1 for β-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 16890162
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Furthermore, we demonstrate that TGFbeta dependent activation of LEF1 and TCF target genes can occur in the absence of beta-catenin binding to LEF1 and TCF and requires both Smad and LEF1 and TCF DNA binding sites in the Xtwn promoter."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10890911
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Here we propose that the beta-catenin nuclear import pathway may provide an opportunity for identification of specific drug targets and inhibition of beta-catenin nuclear function, much like the current screening of drugs that block binding of beta-catenin to LEF-1 and TCFs."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 24820952
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Lithium favours lymphoid enhancer factor-1/beta-catenin binding to Gip promoter and diminishes ChIP through T cell factor-4 and histone deacetylase 1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19582394
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "14 Wnt stabilizes cytosolic beta-catenin, which then binds to TCF and LEF1 in the nucleus and recruits transcription factors Brg1 and CREB binding protein to initiate Wnt targeted gene expression."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28277542
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "As beta-catenin binds to LEF1 in the nucleus in the Wnt-canonical pathway, we performed beta-catenin immunostaining."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28693719
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "It has been shown that phosphorylated β-catenin can interact with LEF-1 but cannot form a ternary complex with DNA xref and cannot drive TCF reporter activity xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20862283
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "While beta-catenin can bind directly to both AR and Lef1, GRIP1 can only bind directly to AR; the ability of GRIP1 to associate with and function as a coactivator for Lef1 is entirely dependent on the presence of beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 14638683
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "As β-catenin binds to LEF1 in the nucleus in the Wnt-canonical pathway ( xref ), we performed β-catenin immunostaining."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28693719
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The result confirmed the tight association between beta-catenin and LEF-1 in the nucleus, further confirming activation of the Wnt signaling (XREF_FIG)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22391957
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In this study, it was observed that the overexpression of wild type LEF1, which can interact with β-catenin, decreased the endogenous levels of Notch1 in a dose-dependent manner (left panel of A)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19000719
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Chromatin immunoprecipitation (ChIP) analysis also showed that the T-cell factor (TCF)4, which is one of the transcription factors of the lymphoid enhancer factor (LEF)/TCF family that binds to stabilized β-catenin to regulate Wnt signalling xref , is bound to the promoter of Irs1 xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27708333
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Wnt factors including beta-catenin, glycogen synthase kinase (GSK3) beta, the lymphoid enhancer binding factor 1 (LEF1) and cyclin D1 have been identified as interacting with the androgenic signaling axis [XREF_BIBR, XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23667383
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "LEF1 interacts with beta-catenin and plays critical role in proliferation of RCC by activating downstream target genes [XREF_BIBR, XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27766950
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "LEF1 interacts with β-catenin and plays critical role in proliferation of RCC by activating downstream target genes [ xref , xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27766950
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "β-catenin may increase FoxO transcriptional activity and competitively limit β-catenin interaction with members of the lymphoid enhancer factor/T cell factor family. xref This may lead to cell injury, since β-catenin has been demonstrated to be necessary for protection against Aβ toxicity in neuronal cells. xref "
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20716915
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Wnt/β-catenin pathway activation is characterized by (i) stabilization of cytoplasmic β-catenin upon receptor engagement by Wnt ligands, (ii) β-catenin nuclear translocalion, (iii) β-catenin interaction with lymphoid enhancer-binding factor-1/T-cell factor-1 (LEF/TCF) transcription factors, and (iv) stimulation of target genes xref , xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20852629
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Beta-catenin and TCF -1 pathway in T cell development and differentiation : The discovery of the association between beta-catenin and LEF-1 set the stage for the realization that beta-catenin is found in the nucleus and has a role as a transcriptional co-regulator."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23114888
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Moreover, RAC1 nuclear accumulation in complex with β-catenin occurs following Wnt or RAC1 activation, resulting in RAC1-dependent phosphorylation of β-catenin on serines 161 and 605, promoting the formation of nuclear β-catenin-lymphoid enhancer factor (LEF-1) complexes, necessary for transactivation of Wnt-dependent genes [ 42 ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29723737
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This allows the direct interaction of β-catenin with the lymphoid enhancer factor-T cell factor family of transcription factors and the promotion of cells into the cell cycle ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 24765201
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "On the other hand, interactions between Runx2 and beta-catenin and Lef1 inhibit Runx2 mediated activation of osteocalcin [XREF_BIBR], and Runx3 was recently shown to attenuate tumorogenic beta-catenin signaling via direct physical interactions with both beta-catenin and TCF4 [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19330799
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The bHLH-LZ region of MITF-M is responsible for the physical interaction with LEF-1, and beta-catenin is required for the collaboration between LEF-1 and MITF-M. Importantly, MITF-M could function as a non-DNA-binding co-factor for LEF-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12753399
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Nuclear β-catenin interacts with members of the Lymphoid Enhancer Factor/T-cell factor (LEF/TCF) family of transcription factors and also with Legless family docking proteins BCL9/BCL9L, which associate with the transcription co-activator pygopus (PYGO) 1/2 ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20200414
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Interaction of β-catenin with transcription factors of the lymphoid enhancer-binding factor/T cell factor family in the nucleus subsequently regulate the transcription of Wnt target genes ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19723499
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Subsequent EMSA and ChIP analysis confirmed the binding of Ets-1 and LEF-1 and beta-catenin transcription factors to the predicted sites."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22952895
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Regarding the putative role of the LEF1 gene in hernia, it has been shown that the LEF1 protein constitutively associates with beta-catenin which mediates the action of the anti-mullerian hormone (AMH) [XREF_BIBR], itself involved in the gubernaculum swelling reaction that occurs during the first phase of testicular descent [XREF_BIBR, XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25886970
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Regarding the putative role of the LEF1 gene in hernia, it has been shown that the LEF1 protein constitutively associates with β-catenin which mediates the action of the anti-mullerian hormone (AMH) [ xref ], itself involved in the gubernaculum swelling reaction that occurs during the first phase of testicular descent [ xref , xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25886970
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ No evidence text available
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12748295
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ No evidence text available
+
+
+
+
+
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+ 15684397
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+ No evidence text available
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+ 10890911
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+ No evidence text available
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+ 8757136
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+ No evidence text available
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+ 8757136
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+ No evidence text available
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+ 16510874
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+ No evidence text available
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+ 16510875
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+ No evidence text available
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+ 16204248
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+ No evidence text available
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+ 10958684
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+ No evidence text available
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+ 11835406
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+ No evidence text available
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+ 15525529
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+ No evidence text available
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+ 11751639
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+ No evidence text available
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+ 12077367
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+ No evidence text available
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+ 18296023
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+ No evidence text available
+
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+ 15768032
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+ No evidence text available
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+ 9419974
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+ No evidence text available
+
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+ 9419974
+
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+
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+ No evidence text available
+
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+ 15728254
+
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+ No evidence text available
+
+
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+
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+
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+ 21743491
+
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+ No evidence text available
+
+
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+
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+
+
+
+
+
+ 21743491
+
+
+
+
+
+
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+
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+ No evidence text available
+
+
+
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+
+
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+
+
+
+
+
+
+ 12556497
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ No evidence text available
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 8757136
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ No evidence text available
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 15684397
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "MALDI-TOF mass spectrometry (MS) identified two CK2 phosphorylation sites (S42, S61) within the amino terminus of hLEF-1, and mutation of these sites reduced binding to beta-catenin in vitro and transactivation in vivo."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17113388
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "MALDI-TOF mass spectrometry (MS) identified two CK2 phosphorylation sites (S42, S61) within the amino terminus of hLEF-1, and mutation of these sites reduced binding to beta-catenin in vitro and transactivation in vivo."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17113388
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "MALDI-TOF mass spectrometry (MS) identified two CK2 phosphorylation sites (S42, S61) within the amino terminus of hLEF-1, and mutation of these sites reduced binding to beta-catenin in vitro and transactivation in vivo."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17113388
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "To develop an enzyme linked immunosorbent assay (ELISA)-based high throughput screening (HTS) method for beta-catenin and Lef1 interaction antagonists screening, Escherichia coli Rosetta (DE3) competent cells were transformed with beta-catenin-pET-30a (+) plasmid."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31001956
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Taken together, we have successfully developed a simple, robust and reliable ELISA based HTS method for screening of novel Wnt inhibitors targeting beta-catenin and Lef1 interaction."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31001956
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the nucleus, β-catenin interacts with members of the lymphoid enhancer factor/T cell factor (LEF/TCF) protein family () and also with other transcription factors, such as Forkhead box protein O1 (FoxO1), hypoxia-inducible factor (HIF), Smads, and others (for review, see )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 24044891
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Beta-catenin bound the transcription factors Tcf7 and Lef1, which promoted transcription of targeted genes, as evidenced by the increased gene expression of Jun, Fzd7, and Nlk after TWS119 treatment."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31119051
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Other work has shown that c-KIT stabilises β-catenin expression [ xref ] which forms a complex with LEF-1 to induce cyclin D1 expression [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27769165
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "When LEF-1 interacts with CTNNB1, it leads to upregulation of LEF-1 in SPN."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 30283597
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "When LEF-1 interacts with CTNNB1, it leads to upregulation of LEF-1 in SPN."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 30283597
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Nuclear translocation of beta-catenin binds to the DNA binding proteins Tcf and Lef1 that subsequently regulates target genes including CyclinD1 important in proliferation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22391317
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We recently identified and characterized a novel Wnt like effect of transforming growth factor-beta (TGF-beta) on beta-catenin, the binding partner of Lef-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 15736165
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Maetzel et al ( xref ) firstly demonstrated that a proteolytic fragment of EpCAM containing EpICD forms a complex with β-catenin and LEF-1, which translocates to the nucleus and activates the transcription of genes associated with cell proliferation, such as c-Myc and cyclins A and E, thereby promoting oncogenesis ( xref ) ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 30015855
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The two pathways were found to converge at the promoter of Xtwn, where Smad4, beta-catenin, and Lef1 form a complex and bind to adjacent regulatory elements to co-regulate Xtwn expression."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19002158
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "have demonstrated that in vitro transcriptional activation by recombinant beta-catenin and LEF -1 complexes requires ubiquitin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21118991
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Here, we present ITC measurements of Lef-1 and Tcf4 binding to beta-catenin under identical conditions, which show that Lef-1 binds slightly less strongly than Tcf4 to beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21075118
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The extended N-terminal region of Lef-1 binds to beta-catenin using most of the conserved sequence motif Dxthetathetaxphix 2-7 E."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21075118
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Given the difference in the structures of TCF-4 and Lef-1 bound to beta-catenin, it was of interest to compare the thermodynamics of these complexes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21075118
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "However, clinical targeted treatment of the beta-catenin and LEF -1 complex remains relatively ineffective."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26750754
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Thus, we attempted to synthesize a peptide (TAT-NLS-BLBD-6) that could interfere with the interaction of beta-catenin and LEF-1 at nuclei in human breast cancer cells."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26750754
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The beta-catenin and LEF -1 complex is a nuclear response transcription factor and plays an important role in the Wnt and wingless signaling pathway XREF_BIBR."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26750754
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Otherwise, upon Wnt binding to the receptor complex, beta-catenin translocates to the nucleus and binds with the LEF-1 and TCF co-transfection factor."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26750754
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the current study, we synthesized a small peptide to block the interaction between beta-catenin and LEF-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26750754
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In our current study, we synthesized a small peptide TAT-NLS-BLBD-6 (ATDEMIPF), which is a Wnt and beta-catenin signaling inhibitor and prevents the binding of beta-catenin to LEF-1 at the nuclear region of human breast cancer cells."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26750754
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In summary, our study found that TAT-NLS-BLBD-6, a cell penetrating pentapeptide, blocks the interaction between beta-catenin and LEF-1 and also efficiently attenuates tumorigenesis in vitro and in vivo."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26750754
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "These results provide evidence that Lef1 promoted the differentiation of bulge stem cells into a hair fate and interacted directly with beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23630438
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We also report that Lef1 could bind to beta-catenin directly and translocate it into the nucleus, resulting in the activation of the Wnt signaling pathway."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23630438
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "There is evidence that beta-catenin can act as a nuclear import receptor to induce the nuclear translocation of Lef1 XREF_BIBR, indicating a close interaction between Lef1 and beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23630438
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In vitro phosphorylation of purified Lef-1 was used to examine the effect of CK2 on the interaction of Lef-1 with β-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21075118
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Isothermal titration calorimetry revealed that β-catenin binds to non-phosphorylated or CK2 phosphorylated Lef-1 with the same affinity, which is consistent with the absence of phosphoSer40 interactions in the crystal structure of phosphorylated Lef-1 N-terminal domain bound to β-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21075118
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "These data indicate that the effect of CK2 on the Wnt/β-catenin pathway does not appear to be at the level of the Lef-1–β-catenin interaction."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21075118
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In order to compare the interactions of Tcf family members with β-catenin, we have determined crystal structures of non-phosphorylated and CK2-phosphorylated Lef-1 bound to the armadillo domain of β-catenin and Lef-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21075118
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We also find that CK2 phosphorylation of Lef-1 produces no change in affinity or in the structure of the complex, indicating that the effects of CK2 on β-catenin-mediated transcription are not at the level of the Lef-1–β-catenin interaction."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21075118
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The extended N-terminal region of Lef-1 binds to β-catenin using most of the conserved sequence motif Dxθθxφx 2–7 E. There are relatively few direct contacts between Lef-1 residues 11–18 and β-catenin, which include a salt bridge between Asp12 and Arg515, and the backbone carbonyl oxygen of Gly11 forms a hydrogen bond with Arg582 ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21075118
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In order to assess the possible effects of CK2 phosphorylation on the Lef-1–β-catenin interaction, the complex of the CK2-phosphorylated Lef-1(1–63) and β-cat-arm was purified and crystallized."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21075118
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In this smaller lattice, Lef-1 Asp26, Gly28, Pro30, Gln31, Glu33, Phe36, Ala37, and Glu38 interact directly with neighboring β-catenin molecules ( xref ); note that Glu33 forms a salt bridge with Arg647 of a neighbor as well as Arg386 (see above)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21075118
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Residues 11–18 adopt a conformation different from the equivalent portions of other Tcfs, which may be due to differences in lattice packing, as neighboring molecules of β-catenin interact with some of this region of Lef-1. (Interestingly, pLef-1 Cys16 forms a disulfide bond with β-catenin Cys350 from a lattice neighbor, even though its conformation is the same in the non-phosphorylated structure.) It is also possible that some of the difference arises from the insertion of Pro13 between two acids that are otherwise adjacent in sequence in the other Tcfs ( xref ); Lef-1 Asp12 forms a salt bridge with β-catenin Arg515 ( xref ), whereas the equivalent interaction is absent in other TCF–β-catenin structures."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21075118
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In such treated cells, the association of beta-catenin with Lef-1 and the transcription of c-myc, cyclin-D1, CD44 and COX-2 were also reduced."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 16080193
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "As shown in XREF_FIG, a clear less associated LEF1 was observed in beta-catenin precipitates from DACT2 expressing cells, indicating that the interaction between LEF1 and beta-catenin was evidently impaired in the presence of DACT2."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25023701
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The interaction between beta-catenin and LEF-1 and TCF transcription factors plays a pivotal role in the Wnt-1 signaling pathway."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9874785
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We also report that Lef1 could bind to β-catenin directly and translocate it into the nucleus, resulting in the activation of the Wnt signaling pathway."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23630438
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "There is evidence that β-catenin can act as a nuclear import receptor to induce the nuclear translocation of Lef1 xref , indicating a close interaction between Lef1 and β-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23630438
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Because LEF-1 possesses its own NLS ( Huber et al., 1996 ) and is able to interact with β-catenin, we asked whether glucose favored LEF-1 and β-catenin interaction as a possible mechanism for the nuclear accumulation of β-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Immunoprecipitation experiments indicated that LiCl and glucose cooperate to selectively favor nuclear LEF-1 and β-catenin interaction, and neither glucose nor LiCl alone allowed complex formation ( Figure 2 D)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Whereas ectoptic WT β-catenin bound p300 upon exposure of the cells to both glucose and LiCl (like endogenous β-catenin), conditions that promote LEF-1 and β-catenin interaction and nuclear accumulation of β-catenin, the Δ19 β-catenin mutant was unable to bind p300 under any conditions."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Thus, the LEF-1 and β-catenin interaction promoted by the combination of glucose and LiCl appears critical for stabilizing p300 interaction and nuclear retention."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "At the GIP , GCG , and cyclin D promoters, both LEF-1 and β-catenin were poorly bound in the absence of LiCl."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Single treatment with glucose or LiCl alone induced only minor increases in the binding of both LEF-1 and β-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Increased binding of both LEF-1 and β-catenin at the cyclin D promoter follows combined treatment with glucose and LiCl in HT-29 colon cancer cells."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "First, it increases p300 expression and enhances its interactions with LEF-1 and β-catenin; second, it inhibits sirtuin deacetylase activity."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "K354 lies within the region absent in the Δ19 mutant, a region previously identified as being critical for β-catenin interaction with LEF-1 ( von Kries et al., 2000 )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Although the precise molecular mechanisms have not been explored here, we view it as probable that SIRT1 acts as a competitor for interaction between LEF-1 and β-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Although SIRT1 may play a role via stable and direct binding to LEF-1 or β-catenin, it is also clear that its catalytic activity is particularly important."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "XREF_BIBR While collectively these measurements reveal that phosphorylated APC competes effectively with LEF and TCF for beta-catenin binding, recent data suggests phosphorylation of LEF-1 by CK2 also enhances LEF-1 binding to beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19928349
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "APC binds to CK2 and renders it inactive, leaving LEF-1 in an unphosphorylated form and thereby less able to bind beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19928349
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Jerky also recruits β-catenin to chromatin, and promotes the association of β-catenin and LEF1, resulting in the induction of Wnt target gene expression.( xref , xref ) We found that RUNX3 increases the occupancy of the TCF4/β-catenin complex in KatoIII cells, suggesting that RUNX3 plays a role in the stabilization of the TCF4/β-catenin complex on the Wnt target gene promoter like TBL1 and Jerky."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 24447505
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In this study, HT-29 colon cancer cells with truncated APC have LEF-1 and beta-catenin complexes constitutively occupying a WRE in the cMYC enhancer."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19460168
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Here we show, in a yeast two-hybrid screen, that the architectural transcription factor LEF-1 (for lymphoid enhancer binding factor) interacts with beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 8757136
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In relation to this, it is notable that although lymphoid enhancer factor/TCF7 transcription factors associate with β-catenin in canonical wnt signaling leading to the transcription of its target genes, SATB1 has recently been shown to competitively influence the TCF7 binding to β-catenin ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20467038
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Lef1 can interact with beta-catenin in a transcriptional complex downstream of Wnt signaling XREF_BIBR, and this pathway antagonizes aspects of testis development XREF_BIBR, XREF_BIBR."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22438826
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Association of LEF-1 with β-catenin relieves this inhibition and facilitates LEF-1 interaction with chromatin ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12067647
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Lef1 can interact with β-catenin in a transcriptional complex downstream of Wnt signaling xref , and this pathway antagonizes aspects of testis development xref , xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22438826
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In transgenic mouse models, intact Wnt signaling promotes hair follicle differentiation, whereas inhibition of Wnt signaling through the prevention of Lef1 and beta-catenin interaction leads to sebocyte differentiation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26548488
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Here we show, in a yeast two-hybrid screen, that the architectural transcription factor LEF-1 (for lymphoid enhancer-binding factor) interacts with beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 8757136
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In mammalian cells, coexpressed LEF-1 and beta-catenin form a complex that is localized to the nucleus and can be detected by immunoprecipitation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 8757136
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Moreover, LEF-1 and beta-catenin form a ternary complex with DNA that splays an altered DNA bend."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 8757136
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In vivo, MuSCs underwent a switch from β-catenin-Lef1 to Smad3-Lef1 interactions during the postnatal switch from proliferation to quiescence, with β-catenin-Lef1 interactions recurring during damage-induced reactivation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 30042129
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Further, a significant difference in the interaction between Lef1 and beta-catenin was seen when wild-type cells were compared with knockout cells (XREF_FIG)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27903773
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In NLK-knockout cells, there is a constitutive interaction between Lef1 and beta-catenin, which could explain the elevated Tcf/Lef promoter activity in these cells."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27903773
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Previous studies have found that members of the lymphoid enhancer factor-1/T-cell factor (LEF-1/TCF) family interaction with β-catenin and their roles as the nuclear effectors of Wnt signaling [ xref , xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27835604
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In NLK-knockout cells, we observed sustained interaction between Lef1 and β-catenin, leading to elevated luciferase reporter of β-catenin/Lef1–mediated transcriptional activation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27903773
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Further, a significant difference in the interaction between Lef1 and β-catenin was seen when wild-type cells were compared with knockout cells ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27903773
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "LEF-1 and β-catenin form a ternary complex with DNA that displays an altered DNA bend ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19458225
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Nuclear beta-catenin binds to and activates the LEF-1 and TCF transcription factors to induce expression of genes involved in tumorigenicity and invasion."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 11535620
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Beta-catenin then accumulates in the nucleus and binds LEF-1 and TCF to regulate the expression of Wnt target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25311420
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Functional interaction of beta-catenin with the transcription factor LEF-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 8757136
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "It was shown that Smad4, beta catenin and Lef1 form a complex to activate expression of the Wnt target gene twin (Xtwn) [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19926329
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The downregulation of APC contributes to nuclear accumulation of β-catenin, which subsequently interacts with the lymphoid enhancing factor/T cell factor (TCF) transcription factor to activate the transcription of target genes including c-Myc, cyclin D1, and matrix metalloproteinase 7 (MMP7) [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 30134915
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Thus, unlike polarized cells, in mesenchymal cells LEF-1 and beta-catenin may form complexes within the nucleus."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10629227
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Moreover, a LEF-1 and TCF recognition site has been identified upstream of these elements, which was shown to bind to a beta-catenin and LEF -1 complex in vitro (Huber et al. 1996b)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10629227
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "It remains to be determined whether beta-catenin and LEF -1 complexes recognize this site also in vivo and whether they influence the expression of E-cadherin in a positive or negative fashion."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10629227
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "As many of these structures were also stained by an affinity purified anti-LEF-1 antibody, they likely represent functional beta-catenin and LEF -1 complexes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10629227
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The notion that transcriptionally active beta-catenin and LEF -1 complexes were formed in estradiol treated FosER cells was supported by our observation that LEF-1 and TCF-dependent luciferase reporter activity was clearly elevated in the mesenchymal cells."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10629227
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Thus, activation of FosER and integrin linked kinase might trigger signaling pathways that are linked to the wnt-pathway or regulate the formation of an active nuclear beta-catenin and LEF -1 complex via as yet unknown mechanisms."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10629227
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Thus, unlike polarized cells, in mesenchymal cells LEF-1 and β-catenin may form complexes within the nucleus."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10629227
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Our results clearly demonstrated that endogenous β-catenin and endogenous LEF-1/TCF proteins in mesenchymal FosER cells were able to form a transcriptionally active complex, which could be increased further upon ectopic expression of β-catenin and LEF-1 without additional signals."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10629227
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In addition to TCF4 mainly (Gotoh et al. xref ), LEF-1 (Grueneberg et al. xref ), HBP1 (Sampson et al. xref ), and UBF2 (Grueneberg et al. xref ) can also affect the cyclin D1 promoter activity by interaction with β-catenin or LEF-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22704330
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "However, EpICD does not only interact with β-catenin and Lef-1, it also binds to Lef-1 consensus sites in the promoter of Wnt target genes such as cyclin D1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 24944998
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The interaction between the enamelin elements and beta-catenin and LEF1 was further confirmed by electrophoresis mobility shift assays and chromatin immunoprecipitation assays."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20599763
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Over-expression of ILK in intestinal epithelial cells resulted in the expression of an invasive phenotype, which is consistent with Wnt pathway events such as loss of E-cadherin expression, and nuclear translocation of β-catenin, formation of a lymphoid enhancer factor (LEF)-1/β-catenin complex, and enhanced LEF-1 transcriptional activity (Novak et al., 1998) ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12191615
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Recent implications for beta-catenin signaling in cancer prompted us to examine colon cancer cell lines for the expression of LEF-1, a transcription factor that binds to beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9419974
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Beta-catenin binds TCF-4 or LEF-1 to regulate cognate gene expression."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21744004
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In these cells, the association of endogenous LEF1 and beta-catenin was induced by stimulation with the wnt-1 proto-oncogene."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9419974
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Ectopic overexpression of beta-catenin in 293 cells also induced the assembly of the beta-catenin-LEF-1 complex and activated gene transcription from a LEF-1-dependent promotor."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9419974
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The results suggest that a cancer pathway driven by wnt-1, or mutant forms of beta-catenin, may involve the formation of a persistent transcriptionally active complex of beta-catenin and LEF1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9419974
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "To activate target gene expression, TCF-1 and LEF-1 must first associate with beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23041833
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "[Development of an ELISA based high throughput screening method for novel anticancer agents targeting beta-catenin and Lef1 interaction]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31001956
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Since APC and the HMG transcription factor Lef-1 both bind to the armadillo repeats of beta-catenin, beta-catenin must dissociate from APC and subsequently bind to Lef-1 to allow translocation to the nucleus and transcriptional activation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9362521
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Since APC and the HMG transcription factor Lef-1 both bind to the armadillo repeats of β-catenin ( xref ; xref ), β-catenin must dissociate from APC and subsequently bind to Lef-1 to allow translocation to the nucleus and transcriptional activation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9362521
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "It is possible that α-catenin could abrogate monomeric β-catenin's association with Lef-1 or alternatively stabilize β-catenin's association in the APC complex."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9362521
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Some studies reported promising results in APC mutated CRC cell lines [XREF_BIBR, XREF_BIBR], whilst others showed that nuclear beta-catenin bound to LEF-1 and TCF transcription factors would be protected from degradation such that TNKSi treatment would not be effective [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26930278
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Accumulated unphosphorylated β-catenin translocates to the nucleus and eventually binds to the lymphoid enhancer factor/T cell factor (LEF/TCF) transcription factors to exert effects on downstream gene transcription."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29361730
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Further analysis revealed that SMAR1 overexpression led to Wnt3a dependent TCF4/LEF1 transcriptional inhibition by reducing the association of β-catenin and LEF1 ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29765542
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "hESCs treated with Wnt3A for 6 hours showed rapid binding of beta-catenin and LEF1 to the LGR5 locus as well, indicating that LGR5 is a direct target of these transcription factors (XREF_FIG D)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28078320
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This process results in activation of β-catenin-regulated transcription (CRT), which interacts with transcription factors lymphoid enhancer factor (LEF)/T-cell factor (TCF)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31867287
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Approaches impairing binding of LEF1 to β-catenin significantly impaired AML growth, but spared normal hematopoietic stem cells."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31758089
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The cytoplasmic accumulation of β-catenin after activation of Wnt signaling, or the release of β-catenin due to the loss of E-cadherin, which allow the interactions between β-catenin and LEF-1, can induce the upregulation of Snail, Slug, Twist and mesenchymal markers such as fibronectin ( xref ; xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31849690
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Approaches impairing binding of LEF1 to beta-catenin significantly impaired AML growth, but spared normal hematopoietic stem cells."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31758089
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This report now demonstrates a striking difference of LEF1 isoform expression between normal and AML cells, contributing to higher vulnerability of leukemic cells to approaches targeting beta-catenin and LEF1 interaction."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31758089
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In this report, we present evidence that canonical Wnt and beta-catenin signaling is not required for initial activation of Pitx2, but it is required for maintenance of its continued expression likely via direct binding of Lef-1 and beta-catenin to the Pitx2 promoter."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20960542
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The binding of unphosphorylated beta-catenin to Lef1 in the nucleus to form the transcription activator was modeled using a mass action law (Eq2.11 in the Additional file XREF_SUPPLEMENTARY)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23602012
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Research by Juan Galceran et al. [XREF_BIBR] confirmed that Lef1 binds to beta-catenin in the nucleus to form the Lef1, beta, and catenin complex that activates the expression of endogenous Dll1 in fibroblasts."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23602012
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Using recombinant proteins, we found that E-cadherin and lymphocyte-enhancer factor-1 (LEF-1) form mutually exclusive complexes with beta-catenin; the association of beta-catenin with LEF-1 was competed out by the E-cadherin cytoplasmic domain."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10085258
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In all SCOUTs the expression of LEF1 was associated with a marked
+nuclear expression of β-catenin ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28664938
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Further, chromatin immunoprecipitation (ChIP) assays demonstrate that both beta-catenin and Lef1 bind directly to the neurogenin promoter, and luciferase reporter assays demonstrate that beta-catenin is directly involved in the regulation of neurogenin 1 and possibly other proneural genes when neural stem cells are cultured in the presence of FGF2."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 15031118
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Elevated beta-catenin levels in colorectal cancer result in the binding of beta-catenin to LEF-1 and increased transcriptional activation of the CCND1 gene."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 15557752
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Elevated beta-catenin levels in colorectal cancer result in the binding of beta-catenin to LEF-1 and increased transcriptional activation of the CCND1 gene."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 15557752
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "EA Directly Interacts with LEF-1 and Destabilizes the LEF-1 and beta-Catenin Complex."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20011538
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Intermittent PTH is a pathway agonist, thus, the accumulated β-catenin binds to T-cell-specific transcription factor (TCF) and lymphoid enhancing factor (LEF), displacing the Groucho repressor gene, and thereby allowing the transcription of Wnt-specific osteoblast differentiation genes. xref "
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28062525
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The binding of unphosphorylated β-catenin to Lef1 in the nucleus to form the transcription activator was modeled using a mass action law (Eq 2.11 in the Additional file xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23602012
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The GSK3-Axin2 degradation complex is in antiphase with the β-catenin-Lef1 transcriptional activator, because it degrades β-catenin and thus inhibits the formation of the β-catenin-Lef1 complex."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23602012
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Research by Juan Galceran et al. [ xref ] confirmed that Lef1 binds to β-catenin in the nucleus to form the Lef1-β-catenin complex that activates the expression of endogenous Dll1 in fibroblasts."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23602012
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Elevated beta-catenin levels in colorectal cancer result in the binding of beta-catenin to LEF-1 and increased transcriptional activation of the CCND1 gene."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 15557752
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "It has been demonstrated that LEF-1 is constitutively associated with β-catenin in SW480 cells xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20011538
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "OSTM1 acts also as an E3 ubiquitin ligase for the heterotrimeric G protein Galphai3 and potentiates WNT canonical signaling by modulating beta-catenin and Lef1 interaction."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 30837952
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In addition, Wnt signaling activated target gene expression through the interaction of beta-catenin and a LEF-1 and TCF transcription factor family member [XREF_BIBR, XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26580798
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "It has been shown that CK1delta and CK2 phosphorylate Lef-1 [XREF_BIBR] leading to a disruption of interactions between beta-catenin and Lef-1, but not between Lef-1 and the template DNA."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23016862
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "During a high-throughput screening the approved FDA diuretic ethacrynic acid was found to down-regulate Wnt and beta-catenin signaling by inhibiting the formation of the beta-catenin and Lef -1 complex [XREF_BIBR, XREF_BIBR] in chronic lymphocytic leukemia (CLL) cells, although at a low IC 50."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23016862
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Cross-talk with the Wnt pathway is possible at the level of beta-catenin and Lef-1 interactions with EpICD, and known for induction of the EPCAM promoter by Tcf4."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21552419
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Chibby (Cby) inhibits Wnt and beta-catenin-mediated transcriptional activation by competing with Lef-1 (the transcription factor and target of beta-catenin) to bind to beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25175341
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Our research revealed a novel interaction between PITX2, Lef-1, and beta-catenin in which the Lef-1 beta-catenin binding domain is dispensable for its interaction with PITX2."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17785445
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "3D complex structures show that CDH1, LEF1 and TCF7L2 bind to beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27928465
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "LMNADelta9 expressing cells showed a 70% reduction in the nuclear levels of Tcf and Lef1 binding to beta-catenin compared to controls, indicating that Delta9 reduced the levels of active Tcf and Lef-beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20833363
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "These data support a role for PITX2 in cell proliferation, migration, and cell division through differential Lef-1 isoform expression and interactions with Lef-1 and beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17785445
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Cross-talk with the Wnt pathway is possible at the level of β-catenin and Lef-1 interactions with EpICD, and known for induction of the EPCAM promoter by Tcf4."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21552419
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This approach has been used to inhibit WNT signaling with a β-catenin-ENGR repressor and relies on β-catenin's association with the activator LEF-1, which provides the DNA-binding and promoter targeting function ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 11164187
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Dact proteins have been shown to also modulate Wnt signaling mediators in a ligand independent fashion : Dact proteins shuttle between the nucleus and cytoplasm, and can block nuclear beta-Catenin function by disrupting beta-Catenin and Lef1 complexes and enhancing Lef1-HDAC interaction [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25099342
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Electrophoretic mobility shift assays (EMSAs) confirmed binding of beta-catenin and Lef1 to the TBE2 site (XREF_FIG)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 24101380
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The ESR1 and beta-catenin conjugation then associated with the transcription factors TCF3 and LEF1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26432349
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Free beta-catenin interacts with the DNA binding proteins Tcf3 and Lef1 to form a bipartite transactivator that stimulates the transcription of immediate gene targets."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 11524435
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In many systems, Lef1 interaction with β-catenin is necessary for Lef1-mediated activation of target genes (reviewed in Behrens et al. 1996; Clevers and van de Wetering, 1997 )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 18280717
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Active beta-catenin binds to LEF1 to form transcription complexes to activate target gene expression."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27441654
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Upon translocation to the nucleus, beta-catenin interacts with Tcf4 and Lef1 to regulate a wide range of gene expression at the transcriptional level [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20858269
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "10 As a complex, Smad2/3-Smad 4 are then able to enter the nucleus of the epithelial cell and increase transcription of LEF-1, a member of the T-cell family of transcription factors that binds with beta-catenin suppressing transcription of epithelial markers such as E-cadherin and promoting transcription of mesenchymal markers such as vimentin and N-cadherin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22561306
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "However, it has also been reported that the inhibition of interaction between β-catenin and Lef-1 caused HFSCs to differentiate into sebocytes xref , xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25076848
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The role of beta-catenin in anoikis is also attributable to the activation of the Wnt pathway, in which beta-catenin interacts with transcription factor lymphoid enhancer binding factor 1 (LEF-1) to regulate gene expression and subsequently inhibit anoikis [XREF_BIBR - XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26587543
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In addition, beta-catenin also translocates into the nucleus and interacts with LEF-1 and TCF-4 at the COX2 promoter to activate the transcription of COX2."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28587064
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "However, as β-catenin accumulates in the nucleus, Groucho/TLE is displaced from Tcf/Lef by β-catenin binding to C-terminal of Lef1 (residure252-397)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20858269
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Lef1 binding site was mapped to the 3 ' region of the MMP13 genomic locus and binding of Lef1 and beta-catenin to the site was confirmed by chromatin immunoprecipitation (ChIP) assays and electrophoretic mobility shift assays (EMSAs)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17971297
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Furthermore, Lef1 and beta-catenin binding to the Lef1 binding site transactivated MMP13 promoter activity."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17971297
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Upon entering the nucleus, beta-catenin replaces Groucho/TLE, forms complexes with TCF-1 and LEF-1, and activates the transcription of Wnt target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20026746
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In line with our previous findings that DBC1 enhances LEF1-β-catenin complex formation on WREs of β-catenin targets including a Wnt-inducible transcription factor PROX1 involved in colon cancer progression xref , DBC1 increases LEF1-β-catenin interaction on the MACC1 enhancer, and loss of DBC1 reduced the occupancy of LEF1, TCF4, and β-catenin on the MACC1 enhancer."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 30082743
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Akt dependent release of FoxO1 and glucose dependent binding of LEF1 and beta-catenin mediate induction of Gip expression while insulin induced phosphorylation of beta-catenin does not alter its localization or transcriptional activity in enteroendocrine cells."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25091498
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "However, unlike embryonic development in which uniform upper dermal Wnt signaling precedes hair morphogenesis ( xref ; xref ; xref ) we could not detect Wnt activity in the upper dermis prior to hair germ formation by either β-catenin or LEF-1 immunohistochemistry or with a Wnt-responsive reporter ( xref , data not shown)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22810306
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "TGF-beta1 induced nuclear Smad3, beta, and catenin complex, but not beta-catenin and LEF -1 complex or TOP-flash activity, during EMT of C1.1 (renal tubular epithelial) cells."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23203799
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "During hair follicle development in the mouse, epithelial cells of the ectoderm secrete Wnt3a, leading to nuclear accumulation of beta-Catenin, and mesenchymal cells secrete Noggin, which activates the Lef1 transcription factor; Lef1 can subsequently form a heterodimer with beta-Catenin to regulate transcription of target genes XREF_BIBR."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17895986
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Recent biochemical studies indicate that the vertebrate HMG-domain proteins Lef-1 and XTcf-3 can physically interact with beta-catenin, a homologue of Drosophila Armadillo (Arm), the most downstream component known in the Wnt signal transduction pathway."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9039917
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "During gastrulation, beta-catenin forms a complex with LEF-1 to bind and inhibit the transcription of CDH1 (XREF_FIG) and induce EMT."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25249658
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "During gastrulation, β-catenin forms a complex with LEF-1 to bind and inhibit the transcription of CDH1 ( xref ) and induce EMT ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25249658
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "beta-Catenin and LEF-1 complexes can activate reporter gene expression in a transformed T-lymphocyte cell line (Jurkat) but not in normal T lymphocytes, even though both factors are nuclear."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10330189
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This protein inhibits Wnt and beta-catenin mediated transcriptional activation by competing with Lef-1 (the transcription factor and target of beta-catenin) to bind to beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 15245581
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Low levels of YREG1 were important for stabilizing the interaction between LEF-1 and beta-catenin, while elevated levels inhibited EMT by downregulating LEF-1 transcriptional activity."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28027307
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This result suggests that beta-catenin loses its ability to translocate into the nucleus and bind to LEF-1 and TCF-4 following TQ treatment."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28275297
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Transcriptome analysis using gene sets related to the regulation of nuclear beta-catenin signaling and target gene transcription did not reveal a differential expression of the beta-catenin interaction partners LEF1 or TCF7L2 (TCF-4)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27428425
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "As discussed above, elevated cytosolic β-catenin levels are associated with increased β-catenin nuclear accumulation and LEF-1-mediated transcription, hence the implication is that presenilin-mediated proteolytic cleavage of E-cadherin may contribute to enhanced transcription of genes involved in survival."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12527355
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Also, LEF1 may form complexes with beta-catenin (CTNNB1), T-Cell Factor 4 (TCF7L2), and other proteins (HINT1, RUVBL1) implicated in Wnt / beta-catenin signaling, a crucial developmental pathway [XREF_BIBR - XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28376810
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Surprisingly, however, phosphorylation of murine Ser40 residue (corresponding to human Ser42) by CKIdelta disrupts the beta-catenin and LEF -1 complex [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 24594309
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Wnt and beta-catenin pathway activation is characterized by (i) stabilization of cytoplasmic beta-catenin upon receptor engagement by Wnt ligands, (ii) beta-catenin nuclear translocalion, (iii) beta-catenin interaction with lymphoid enhancer binding factor-1 and T-cell factor-1 (LEF and TCF) transcription factors, and (iv) stimulation of target genes XREF_BIBR, XREF_BIBR."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20852629
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Nuclear β-catenin associates with the Lymphoid Enhancer Factor/T-Cell-Specific Transcription Factor (LEF/TCF) family of transcription factors and activates transcription of Wnt target genes (Reviewed in [ xref ])."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21416313
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Because proinflammatory cytokine induced cartilage degradation appears to involve WNT and beta-catenin signaling and increased WNT and beta-catenin signaling has been implicated in the initiation and progressive deterioration of cartilage degeneration, we hypothesized that small molecule inhibitors of the interaction between beta-catenin and TCF4 and Lef1 could be used to prevent cytokine induced cartilage degradation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23965253
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the presence of Wnt, the β-catenin transcriptional co-activator translocates to the nucleus and associates with members of the T-cell factor/ lymphoid enhancer factor (TCF/LEF; hereafter, TCF) family of transcription factors [ xref , xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 30794691
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Subsequently β-catenin interacts with members of the lymphoid enhancer factor 1/T-cell factor (LEF1/TCF) family, resulting in generation of a functional transcription factor complex and the expression of downstream target genes. [ xref ] Non-canonical Wnt ligands bind to FZD family receptors, and ROR2 and RYK co-receptors.[ xref – xref ] This signaling is mainly involved in cytoskeletal reorganization during cancer cell invasion and metastasis.[ xref ][ xref ] At present, five Wnt antagonist families have been described, namely, secreted frizzled-related protein (sFRP), Wnt inhibitory factor 1 (Wif1), Xenopus Cerberus, Wise and Dickkopf (DKK) families.[ xref ]"
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19562778
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Further downstream, CK2 is capable of regulating the interaction and transactivation activity of a beta-catenin and LEF -1 complex though phosphorylation of LEF-1 at S42, S61 [XREF_BIBR, XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19387549
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Beta-catenin and LEF -1 complexes target motifs at -75 and -15 within the CCND1 promoter [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27695879
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "On the other hand, a strong binding of beta-catenin to cadherin, rather than to LEF-1, may result in its cytoplasmic sequestration and the inhibition of transactivation by it."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9628899
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the canonical Wnt signaling pathway, the beta-catenin in the nucleus forms a complex with LEF1 to activate transcription of the target gene [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29120400
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The upregulation of the above genes takes place in a direct manner, as we demonstrated, conserved LEF binding sites in MMP-9, 13, 14, ADAMTS-5, and COL10A1 promoters, responsible for their promoter activity and is associated directly with the beta-catenin and LEF -1 complex."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22513174
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Binding of beta-catenin to cadherin, APC protein, and LEF-1 indicates divergent functions of beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9199178
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "TCF1 and LEF1 can bind beta-catenin and activate target genes when the Wnt pathway is active."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 24936456
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "103 Similarly, the absence of beta-catenin and LEF1 interaction, via mutations of LEF1 within the N-terminal beta-catenin binding domain, is associated with increased sebaceous cell differentiation and development of sebaceous tumors in humans."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22111711
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "CK1delta and CK2 phosphorylations of LEF1 on S40 in the N-terminal beta-catenin binding domain XREF_BIBR, XREF_BIBR are dispensable for beta-catenin and LEF1 interaction in vitro, 120 and thus their regulatory roles on LEF1 activity remain to be confirmed."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22111711
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In CRC, ILK induces β-catenin–LEF1 interaction and transactivation, the loss of E-cadherin via induction of EMT-TFs, and the induction of stem cell markers xref – xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 30271576
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "AEG-1 facilitated β-catenin translocation into the nucleus by forming a complex with LEF1 and β-catenin, subsequently activating Wnt signaling downstream genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27903708
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Second, once HDAC1 dependent repression has been overridden, beta-catenin binds LEF1 and the beta, catenin, and LEF1 complex is competent to activate the expression of downstream target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10958684
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Second, once HDAC1-dependent repression has been overridden, beta-catenin binds LEF1 and the beta-catenin-LEF1 complex is competent to activate the expression of downstream target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10958684
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "However, it has also been reported that the inhibition of interaction between beta-catenin and Lef-1 caused HFSCs to differentiate into sebocytes XREF_BIBR, XREF_BIBR."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25076848
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Surprisingly, beta-catenin directly interacts with the transcription factor LEF-1 and XTCF-3, and thereby is not only translocated into the nucleus but also modulates the properties of LEF-1 and XTCF-3 as a transcription factor."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9046239
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Members of the Lef1 and Tcf family of DNA binding proteins bind beta-catenin and transactivate Wnt target genes XREF_BIBR."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19718027
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "XREF_BIBR - XREF_BIBR Although it is well established that the formation of nuclear beta-catenin and LEF1 complexes has a crucial role in the activation of Wnt target genes, the details of the regulatory mechanisms of beta-catenin activity and its interaction with LEF1 are still under investigation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26477307
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Furthermore, endogenous interaction between beta-catenin and LEF1 was decreased in DBC1 KO SW480 cells (XREF_FIG), suggesting that DBC1 stabilizes the interaction between beta-catenin and LEF1 by interacting with beta-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26477307
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Consistent with the model in which beta-catenin acetylation enhances its binding to LEF1 and leads to increased chromatin occupancy of LEF1 by stabilizing LEF1, beta, and catenin complex, XREF_BIBR, XREF_BIBR our results further showed that DBC1 enhances the interaction of beta-catenin with LEF1 (XREF_FIG) and is required for efficient recruitment of LEF1 and beta-catenin to their target chromatin regions (XREF_FIG), probably through protecting beta-catenin from SIRT1 mediated deacetylation (XREF_FIG)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26477307
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "These results suggest that DBC1 is required for efficient binding of LEF1 to its regulatory regions and/or for stable association of LEF1 with β-catenin, which strongly enhances the binding of LEF1 to chromatin, xref and thereby facilitates the occupancy of p300 and Pol II on the regulatory regions of LEF1–β-catenin target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26477307
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Consistent with these results, DBC1 increased the interaction of β-catenin with LEF1 in vivo and in vitro ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26477307
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Furthermore, endogenous interaction between β-catenin and LEF1 was decreased in DBC1 KO SW480 cells ( xref ), suggesting that DBC1 stabilizes the interaction between β-catenin and LEF1 by interacting with β-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26477307
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Together, these results suggest that DBC1 functions as a positive regulator of β-catenin by inhibiting SIRT1-mediated deacetylation and repression of β-catenin through blocking the interaction between β-catenin and SIRT1, thereby enhancing the association of β-catenin with LEF1 and promoting β-catenin-mediated transcription ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26477307
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Consistent with the model in which β-catenin acetylation enhances its binding to LEF1 and leads to increased chromatin occupancy of LEF1 by stabilizing LEF1–β-catenin complex, xref , xref our results further showed that DBC1 enhances the interaction of β-catenin with LEF1 ( xref ) and is required for efficient recruitment of LEF1 and β-catenin to their target chromatin regions ( xref ), probably through protecting β-catenin from SIRT1-mediated deacetylation ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26477307
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Upon translocation to the nucleus, beta-catenin interacts with T-cell factor 4 (TCF4) and lymphoid enhancer binding factor 1 (LEF1) to regulate the expression of a wide range of genes at the transcriptional level 12."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28811522
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Protein protein interaction between beta-catenin and Lef-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 14520463
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Immunoprecipitation was performed in order to detect a physical interaction between beta-catenin, alpha-catenin and Lef-1 (XREF_FIG)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 14520463
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Elevated beta-catenin levels in colorectal cancer caused by mutations in beta-catenin or by the adenomatous polyposis coli molecule, which regulates beta-catenin degradation, result in the binding of beta-catenin to LEF-1 and increased transcriptional activation of mostly unknown target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10318916
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Specificity of the small molecules was demonstrated by coimmunoprecipitation experiments for the lef-1/beta-catenin interaction."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20360943
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Coimmunoprecipitation showed a selective disruption of beta-catenin/lef-1 interaction."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20360943
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Elevated beta-catenin levels in colorectal cancer caused by mutations in beta-catenin or by the adenomatous polyposis coli molecule, which regulates beta-catenin degradation, result in the binding of beta-catenin to LEF-1 and increased transcriptional activation of mostly unknown target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10318916
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Beta-catenin forms a complex with LEF1 and TCFs and transactivates LEF1 and TCF mediated transcriptions during dorsalization."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17404593
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We observe similar molecular interactions between Lef1 and beta-catenin and vertebrate Hipk2, suggesting a direct and conserved role for Hipk proteins in promoting Wnt signaling."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19088090
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "When beta-catenin binds TCF and Lef1, it can regulate the transcription of Wnt target genes [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27999816
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Lef1 is a member the Tcf/Lef family of transcription factors and interacts with beta-catenin to up-regulate Wnt target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 30872674
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Specificity of the small molecules was demonstrated by coimmunoprecipitation experiments for the lef-1 and beta-catenin interaction."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20360943
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Coimmunoprecipitation showed a selective disruption of beta-catenin and lef -1 interaction."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20360943
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "After nuclear translocation, beta-catenin binds to DNA binding proteins Tcf and Lef1 which subsequently regulates target genes including Cyclin D1 [XREF_BIBR] important in proliferation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27750091
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Recent studies indicate that increased levels of beta-catenin correlate with the downregulation of the Wnt and beta-catenin pathway due to the proteasomal degradation of beta-catenin and the subsequent decrease in cyclin D [XREF_BIBR], as the cyclin D1 gene might be a target of the beta-catenin and LEF -1 complex [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23970935
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Nuclear β-catenin binds to LEF1, displacing the Groucho/TLE repressors, resulting in transcriptional activation of downstream target genes (c-MYC, Cyclin D1, and LEF1) in response to activation of canonical WNT signaling [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29535816
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Nuclear beta-catenin binds to LEF1, displacing the Groucho/TLE repressors, resulting in transcriptional activation of downstream target genes (c-MYC, Cyclin D1, and LEF1) in response to activation of canonical WNT signaling [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29535816
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Proteolysis of EpCAM leads EpICD to form complex withFHL2, β-catenin and Lef1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26176230
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "To directly assay for binding of LEF-1 and β-catenin to these sites, electrophoretic gel-mobility shift analysis was performed with recombinant LEF-1 and β-catenin produced in ()."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10704849
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Accordingly, ectopic LEF1 expression increases β-catenin binding to TCF-binding sequences and activates the MMP7 promoter [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28757546
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "β-Catenin in the nucleus interacts with transcription factor T-cell factor (TCF) and/or lymphoid enhancer factor (LEF), promoting expression of specific genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26109834
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Interaction between beta-catenin and LEF-1 was assessed in vivo in C3H10T1/2 SS18-SSX-V5 and C3H10T1/2 pLIVc cells by proximity ligation assay (PLA), using a polyclonal goat anti-LEF-1 and a mouse anti-beta-catenin antibody."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26905812
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Wnt3a stimulation of C3H10T1/2 pLIVc (0.1 muM for 16h) did not result in significant dissociation of HDAC from LEF-1 (not shown) whereas it increased association between LEF-1 and beta-catenin, as discussed earlier (XREF_FIG)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26905812
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Moreover, when the endogenous Smad7 was repressed by specific small interfering RNA, TGF-beta-induced increase of activated p38, Akt phosphorylated on Ser473, glycogen synthase kinase 3beta phosphorylated on Ser9 was prevented, as well as the TGF-beta-induced association between beta-catenin and LEF1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 15684397
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Interaction between β-catenin and LEF-1 was assessed in vivo in C3H10T1/2 SS18-SSX-V5 and C3H10T1/2 pLIVc cells by proximity ligation assay (PLA), using a polyclonal goat anti-LEF-1 and a mouse anti-β-catenin antibody."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26905812
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "HQ blocked nuclear translocation of β-catenin and lymphoid enhancer-binding factor 1 (LEF-1), and importantly, HQ also reduced the interaction of β-catenin and LEF-1 in the nucleus."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28886972
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "To determine whether the in silico identified LEF-1 and TCF binding sequences physically bind to the beta-catenin and Lef -1 complex in vitro, we conducted a competitive electrophoretic mobility-shift assay (EMSA) with a radioactively labeled consensus LEF-1 and TCF strong binding probe and duplex oligonucleotides covering the potential sites in target promoters."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21437251
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Hes-1 has recently also been identified as a direct transcriptional target of beta-catenin and Tcf-4-dependent Wnt signaling in CRC XREF_BIBR and our EMSA data supports this notion by clearly identifying Hes-1 -528 as a binding site for the beta-catenin and Lef -1 complex (XREF_SUPPLEMENTARY)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21437251
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In this study, we show that the beta-catenin and Lef -1 complex is capable of binding to the Jagged-1 promoter in vitro at positions -1933, -1635 and possibly -1083 relative to the translation start site (XREF_FIG and XREF_SUPPLEMENTARY) making these plausible sites for transcriptional interactions between Jagged-1 and the canonical Wnt pathway."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21437251
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "HQ blocked nuclear translocation of beta-catenin and lymphoid enhancer binding factor 1 (LEF-1), and importantly, HQ also reduced the interaction of beta-catenin and LEF-1 in the nucleus."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28886972
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Taken together, our results suggest that HQ induced hematotoxicity in bone marrow mononuclear cells is associated with dysregulation of Akt/GSK-3beta/beta-catenin signaling due to the dissociation of beta-catenin and LEF -1 complex, and LiCl and IGF-1 may be two potential agents to ameliorate HQ induced hematotoxicity."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28886972
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In this scenario, tumor cells with activated EpCAM i.e. the released EpICD associates with FHL2, β-catenin which form a nuclear complex with Lef-1 and induces transcription of genes associated with cell proliferation. (Figure xref ; Schematic diagram modified from Maetzel D et al., 2009) [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28903370
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The expressed LEF1 then associates with beta-catenin to drive further expression of full-length LEF1 [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26509276
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Moreover, CK1-dependent phosphorylation in contrast to CK2 disrupts the association of beta-catenin and LEF-1 but does not impair DNA binding of LEF-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 15747065
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Moreover, β-catenin, a protein found at cell–cell adherens junctions, has been shown to play a role in both linking the actin cytoskeleton to the cadherin class of homophilic cell adhesion receptors as well as directly transducing Wnt/ Wingless signals ( xref ; xref ; xref ); Wnt/Wingless signaling results in the nuclear accumulation of a β-catenin-Lef-1 transcription factor complex that binds the promoter regions of several genes (Behrens, et al., 1996; Huber, et al., 1996 b )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9281590
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Transcription factor LEF-1, which acts as a dimer, binds beta-catenin and is involved in signal transduction by the wnt pathway."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 11440173
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "TCF1, LEF1 and TCF4 can bind beta-catenin and activate transcription of target genes as a result of Wnt signaling."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29443926
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In colon cancer cells, beta-catenin and LEF1 complexes drive transcription of the full-length LEF1 isoform that can bind beta-catenin at the expense of a dominant negative isoform."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26509276
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The expressed LEF1 then associates with beta-catenin to drive further expression of full-length LEF1 [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26509276
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the present study, we explored the mechanism (s) through which NO exerts its effect on cytosolic beta-catenin accumulation and nuclear beta-catenin and LEF -1 complex formation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12507936
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Furthermore, the degradation of E-cadherin and the release of beta-catenin to cytosol were accompanied by the formation of nuclear beta-catenin and LEF -1 complex, demonstrating the dissociation of beta-catenin from E-cadherin may be responsible for the activation of beta-catenin and LEF -1 transcription complex."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12507936
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "These MMP inhibitors also blocked the cytosolic accumulation of beta-catenin and nuclear formation of beta-catenin and LEF -1 complex."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12507936
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The sum effect of MMP inhibitors demonstrated that NO induced activation of MMP may cause the degradation of E-cadherin and the subsequent dissociation of beta-catenin, thereby contributing to the cytosolic accumulation of beta-catenin and nuclear formation of beta-catenin and LEF -1 complex."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12507936
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "One possible explanation is that over-inhibition of GSK3 increases the expression of the canonical Wnt pathway effector LEF1, and elevated LEF1 interacts with beta-catenin and leads to activation of lineage specification genes Cdx2 and T [XREF_BIBR] (XREF_FIG)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25595304
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "41
+ In sharp contrast, smad4, another effector of TGF-β, has been shown to form a complex with β-catenin and LEF-1 and to increase β-catenin/LEF-1 transcriptional activity in a hepatoma cell line."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 11839557
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Mechanistically, TCF4 and LEF1 forms a complex with beta-catenin to bind its target gene promoters and promote cancer cell proliferation 28."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29765046
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "What is the structural basis of specific protein-protein interactions, such as those between homeodomain proteins and β-catenin-Lef1 interactions, and how are these determinants altered in transcriptional regulation in oncogenesis and in genetic diseases?"
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10456033
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "As mentioned above, their second selected target, Lef-1, binds nuclear β-catenin in Wnt signaling (section 3.2)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23531534
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Activated dvl binds and inhibits the phosphorylation of beta catenin by gsk3beta/alfa, blocking beta catenin degradation), so that beta catenin accumulates and translocates to the nucleus, where it interacts with the t cell specific factor (tcf)/lymphoid enhancer binding factor 1 (lef-1) transcription factor and induces the transcription of target genes such as c-jun, c-myc, and cyclin d1"
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 15735151
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Active Wnt/β-catenin signalling induces the stabilization of cytosolic β-catenin, which then associates with lymphoid enhancer factor and T-cell factor, such as transcription factor 7-like 2 (TCF7L2 or TCF4) to form a transcription complex that activates Wnt target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22767436
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "To confirm that the compounds inhibit LEF-1 and beta-catenin DNA binding, EMSA assays were performed with immunoprecipitated beta-catenin and a 32 P labeled double stranded deoxyoligonucleotide (5 '-CCCTTTGATCTTACC-3 ') containing the LEF-1 consensus binding site in the presence of varied concentrations of the compounds."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19216569
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "A high proportion of human sebaceous adenomas have amino acid substitutions in the N-terminus of Lef1, which impair the binding of Lef1 to beta-catenin resulting in the inhibition of beta-catenin-dependent transcription of Wnt target genes [XREF_BIBR]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22960253
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "LEF-1 transcription factors are regarded to be the most downstream effectors in the wnt-signalling regulation pathway, forming a complex with β-catenin before binding DNA."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10433970
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Whereas it is recognized that beta-catenin and LEF1 interaction is important in the earlier development of tooth formation, the observed decrease in expression may be associated with the function of Lef1 in the regulation of Enam expression."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22243241
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "A high proportion of human sebaceous adenomas have amino acid substitutions in the N-terminus of Lef1, which impair the binding of Lef1 to β-catenin resulting in the inhibition of β-catenin-dependent transcription of Wnt target genes [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22960253
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Nuclear beta-catenin binds to TCF and Lef1 to induce transcriptional activity, which is a hallmark of Wnt and beta-catenin signaling activation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26663639
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Upon wnt activation, cytoplasmic beta-catenin is stabilized and enters the nucleus, where it associates with transcription factors, notably tcf (t cell factor) and lef (lymphoid enhancer-binding factor), to regulate the transcription of target genes. Thus beta-catenin regulates gene expression by direct interaction with transcription factors such as lef-1, providing a molecular mechanism for the transmission of signals, from cell-adhesion components or wnt protein to the nucleus."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23151663
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Phosphorylated lrp5/6 leads to inhibition of the so-called beta-catenin destruction complex (which includes axin, gsk3, dvl, ck1, and the tumor suppressor adenomatous polyposis coli), resulting in the stabilization and translocation of beta-catenin in the nucleus, where it activates target genes through binding to tcf/lef transcription factors."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21078818
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 14
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The canonical Wnt signaling pathway is one mechanism where β-catenin and Lef-1 can independently interact with PITX2 to increase its transcriptional activity ( xref , xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 18045789
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Because PITX2 interacts with β-catenin and Lef-1 to regulate gene expression we asked if Dact2 interacted with PITX2 to modulate PITX2 transcriptional activity."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23349981
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Pitx2, Lef-1 and β-catenin are associated as a complex when activating downstream gene expression xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23349981
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "It was previously shown that Dact2 inhibits Lef-1 interaction with β-catenin, however, Dact2 does not appear to inhibit the interaction of Pitx2 with β-catenin or Lef-1, but modulates their synergistic transcriptional activities."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23349981
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This regulation of gene expression appears to be linked with Wnt/β-catenin signaling through β-catenin interactions with Lef-1 and Pitx2."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23349981
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Expression of Lef1 is regulated through direct physical interactions of PITX2, LEF1 and β-catenin ( xref ), and PITX2 enhances endogenous expression of the full-length β-catenin-dependent Lef1 isoform while decreasing expression of the N-terminally truncated β-catenin-independent isoform ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20200414
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Our research revealed a novel interaction between PITX2, Lef-1, and beta-catenin in which the Lef-1 beta-catenin binding domain is dispensable for its interaction with PITX2."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17785445
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Lef-1 and beta-catenin interact simultaneously and independently with PITX2 through two different sites to regulate PITX2 transcriptional activity."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17785445
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "LEF-1 and β-catenin bind Pitx2 promoter sequences in ocular neural crest, indicating a likely direct effect of canonical Wnt signaling on Pitx2 expression."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20960542
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In this report, we present evidence that canonical Wnt/ β-catenin signaling is not required for initial activation of Pitx2 , but it is required for maintenance of its continued expression likely via direct binding of Lef-1 and β-catenin to the Pitx2 promoter."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20960542
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Β-catenin and LEF1 bind Pitx2 promoter sequences in developing periocular mesenchyme."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20960542
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Furthermore, beta-catenin in combination with PITX2 synergistically activates the LEF-1 promoter and this activation is independent of the Wnt-responsive element. beta-catenin directly interacts with PITX2 to synergistically regulate LEF-1 expression."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 15728254
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We show a new mechanism where LEF-1 expression is regulated through PITX2, LEF-1 and beta-catenin direct physical interactions."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 15728254
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "LEF-1 and beta-catenin interactions with PITX2 provide new mechanisms for the regulation of PITX2 transcriptional activity."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 15728254
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In addition, the interaction between β-catenin with TCF4 or LEF1 was also enhanced in mPGES-1 overexpressed cells but decreased in mPGES-1 knockdown cells."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21743491
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We observed that in both cases β-CATENIN protein–protein interactions with either LEF-1 or TCF-4 were dramatically decreased after ICG-001 treatment."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23307470
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Our Chromatin immunoprecipitation assay demonstrated a strong binding of β-catenin to the TCF-4 and LEF-1 sites located around -703 and -685 regions of the PDGF-B gene promoter in the KPA cancer cells, while no significant binding was found in the KPC cancer cells (Figure xref D)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 30809277
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Here, we present ITC measurements of Lef-1 and Tcf4 binding to β-catenin under identical conditions, which show that Lef-1 binds slightly less strongly than Tcf4 to β-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21075118
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Given the difference in the structures of TCF-4 and Lef-1 bound to β-catenin, it was of interest to compare the thermodynamics of these complexes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21075118
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Β-catenin binds TCF-4 or LEF-1 to regulate cognate gene expression."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21744004
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In addition, β-catenin also translocates into the nucleus and interacts with LEF-1 and TCF-4 at the COX2 promoter to activate the transcription of COX2."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28587064
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Upon translocation to the nucleus, β-catenin interacts with Tcf4 and Lef1 to regulate a wide range of gene expression at the transcriptional level [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20858269
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Because proinflammatory cytokine-induced cartilage degradation appears to involve WNT/β-catenin signaling and increased WNT/β-catenin signaling has been implicated in the initiation and progressive deterioration of cartilage degeneration, we hypothesized that small molecule inhibitors of the interaction between β-catenin and TCF4 and Lef1 could be used to prevent cytokine-induced cartilage degradation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23965253
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In addition, CGP049090, which blocks the binding of β-catenin to TCF4 and Lef1 [ xref ], was not as effective as PKF115-584."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23965253
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Similarly, the interaction between nuclear β-catenin and TCF-4 or LEF-1 was reduced by CaSR activation ( Fig. 6D ), indicating an inhibitory association of β-catenin and TCF-4 or LEF-1 in the nucleus."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27108064
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "APC mutations and β- catenin gene mutations increase the association of β-catenin with the TCF-4 and Lef-1, xref xref which are involved in the transcriptional regulation of E-cadherin xref and possibly, of other target genes and provide evidence of functional interaction between signaling pathways and cell adhesion molecules during tumorigenesis."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21772890
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Moreover, treatment with the Rac1 inhibitor NSC23766 resulted in a marked reduction in interactions between β-catenin and LEF-1 in the nuclei of Wnt-treated cells ( xref B)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26403202
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Therefore, we want to know whether CHIP influences the interaction between LEF1 and β-catenin which then promotes Wnt signaling cascade."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33431799
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Nuclear RAC1 in complex with β-catenin facilitates RAC1-dependent phosphorylation of β-catenin on serines 161 and 605, resulting in the formation of nuclear β-catenin-LEF-1, which is necessary for the transactivation of Wnt-dependent genes [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 35013128
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "After accumulation, activated β-catenin translocates into the nucleus and forms a complex with the transcriptional factor T-cell factor/ lymphoid enhancer-binding factor 1 (TCF/LEF1) to initiate transcription of Wnt target genes [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28417011
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the presence of Wnt signaling, the accumulated β-catenin in cytoplasm and nucleus interacts with LEF1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19000719
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "β-Catenin can then interact with members of the lymphoid enhancer factor/T-cell factor (LEF/TCF) family of architectural transcription factors in the nucleus, inducing transcription of Wnt-responsive genes (for reviews see xref ; xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10893270
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In response to Wnt signals, β-catenin is stabilized and interacts with the N-terminus of Lef-1 to activate transcription of Wnt-responsive genes ( xref – xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17068080
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Upon translocation to the nucleus, β-catenin forms a complex with transcription factors T-cell specific transcription factor (TCF) and Lymphoid enhancer binding factor (LEF) to drive transcription of key Wnt target genes including Axin2 and Nkd1 ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27083283
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the canonical Wnt signaling pathway, the β-catenin in the nucleus forms a complex with LEF1 to activate transcription of the target gene [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29120400
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the absence of Wnt ligand binding, cytoplasmic β-catenin is degraded by the ubiquitin-proteosome pathway and prevented from entering the nucleus and associating with Lef1 to activate Wnt target genes
+[ xref , xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 24405829
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In turn, the released EpICD associates with components of the Wnt pathway, β-catenin and LEF-1, and regulates gene transcription, inducing proliferation and tumorigenesis in mice."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20851183
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In the canonical Wnt pathway, β-catenin that migrates to the nucleus interacts with two transcription factors, T-cell-transcription factor (TCF) and lymphoid-enhancer-binding factor 1 (LEF-1), to induce the expression of genes that are crucial in the G1–S-phase transition, such as cyclin D1 and c-myc ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12767722
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Buscarlet and colleagues demonstrated that active β-catenin binds to TCF/Lef at a site on Lef-1, where it displaces Groucho/TLE, which overlaps with the Lef-1-binding site. xref Other reports have shown that the modification of TLE by XIAP-mediated ubiquitylation decreases its affinity with β-catenin, thereby allowing the formation of the β-catenin/TCF complex and thus the switch to an activated Wnt signaling pathway. xref The molecular mechanism by which NDRG1 functions in ESCC remains unclear."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27414086
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Nuclear CTNNB1 binds the transcription factors LEF1 (lymphoid enhancer-binding factor 1) and TCF (T-cell factor), thereby converting LEF1 into a transcriptional activator ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23442321
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This allows the formation of complexes of β-catenin with TCF or LEF1, two closely related transcription factors, which then translocate into the nucleus and regulate target gene transcription in response to Wnt ligands."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19153598
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The β-catenin proteins then translocate to the nucleus via BCL9 and Pygo1 [ xref ] where β-catenin interacts with the T-cell factor (TCF) and lymphoid enhancer factor (LEF) transcriptional activators resulting in upregulation of TCF/LEF target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32560059
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "As a TF, β-catenin assembles with the DNA-binding proteins TCF and LEF-1 to activate genes that drive cell proliferation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27050516
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The inactivation of GSK-3β and the subsequent stability and nuclear translocation of β-catenin and formation of a Lef1–Tcf–β-catenin complex plays a fundamental role for the differentiation of the HM cells into the precortical HM and HS ( xref ; xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17485490
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In NLK-knockout cells, there is a constitutive interaction between Lef1 and β-catenin, which could explain the elevated Tcf/Lef promoter activity in these cells."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27903773
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Β-catenin forms complexes with Tcf and Lef-1 and functions as a transcriptional activator in the Wnt signalling pathway."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 11437403
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "When the Wnt signaling pathway is activated, β-catenin interacts with the TCF and lymphoid enhancer–binding factor (LEF) in the nucleus."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27910912
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Consistent with this prediction, we could show the formation of an FHL2–β-catenin–LEF-1 ternary protein complex in a GST pull-down assay with bacterially expressed GST–FHL2 and in vitro–translated myc–β-catenin and HA–LEF-1 ( xref , lane 8)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12370240
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Furthermore, β-catenin is able to bind simultaneously to FHL2 and the transcription factor LEF-1, forming a ternary protein complex."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12370240
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Following RIP, the EpICD fragment is translocated from the cytoplasm into the nucleus and form complex with FHL2, beta-catenin, and Lef-1, stimulating the expression of c-Myc and cyclin A/E genes [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32046162
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "EpICD binds to DNA in a complex with the scaffolding protein FHL2, β-catenin, and Lef-1, and regulates cell proliferation ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21464043
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Maetzel et al ( xref ) revealed that knockdown of EpCAM in FaDu cells results in decreased proliferation, and they concluded that the proliferative action of EpCAM was attributed to the ability of EpICD to form a transcription activator complex with four and a half LIM domains 2, β-Catenin and lymphoid enhancer binding factor 1 (LEF1) ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 30015855
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Released EpICD forms a nuclear complex with FHL2, Lef-1 and β-catenin, activating the downstream signal transduction pathway []."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29981429
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Released EpICD associates with FHL2, β-catenin and Lef-1 to form a nuclear complex and triggers oncogenic signaling."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27176150
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "By inhibiting the cleavage of EpCAM, EpAb2-6 prohibits tumor growth by preventing the translocation of EpICD into the nucleus (Fig. xref ), thus preventing the binding of EpICD to a multiprotein complex consisting of FHL2, β-catenin, and Lef-1, and thereby blocking both the down-regulation of p53 and p21, and the activation of EMT and iPS genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26317650
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The released EpICD forms nuclear protein complex by interacting with the FHL2, β-catenin and Lef1 mediates gene transcriptions and aids in cell proliferation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26176230
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 7
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Activation of β-catenin can also induce the expression of differentiation genes and the induction of these genes in ESCs depends on the interaction of β-catenin with LEF1 and TCF1, two of the four LEF1/TCF family members ( xref ; xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28288968
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Furthermore, overexpression of ICAT, which blocks the binding of β-catenin to TCF-1 and LEF-1, is known to result in a thymocyte development block at the DN to DP transition step in FTOC assays ( xref ), suggesting an essential role for β-catenin/TCF-1 in the DN to DP transition."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22535304
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Both TCF1 and LEF1 interact with β-catenin to regulate the DP (CD4 + CD8 + ) cell differentiation into CD4 + T cells, and in this process, Th-POK is an upstream regulator."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31684152
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "For instance, when ICAT, the inhibitor of β-catenin and TCF, which blocks the binding of β-catenin to TCF-1 and LEF-1, was over-expressed through retroviral transduction, thymocyte development was blocked at the DN to DP transition in FTOC assays, xref indicating an essential role for β-catenin/TCF-1 in the DN to DP transition during thymocyte development."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22321106
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "However, activation of Ctnnb1 can also induce the expression of differentiation genes, such as , and , and the induction of these genes in mESCs mainly depends on the interactions of CTNNB1 with LEF1 and TCF1 (; ; )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28943339
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "TCF1 and LEF1 can bind β-catenin and activate target genes when the Wnt pathway is active ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 24936456
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Studies in vertebrates and Drosophila have led to a model in which Wnt signaling regulates an interaction between beta-catenin and POP-1-related proteins such as Tcf-1 or Lef-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9458047
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 5
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The ubiquitinated LEF1 accelerates the interaction between β-catenin and LEF1, which is further heightened after Wnt3a treatment."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33431799
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We compared the ability of OSTM1 and OSTM1ΔC to regulate the Wnt3a-induced binding of β-catenin to Lef1 ( Fig. 6 )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 18296023
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Β-catenin binding to Lef1 increased in cells treated with Wnt3a ( Fig. 6 , compare lanes 1 and 2)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 18296023
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Overexpression of OSTM1 intensified, while OSTM1ΔC inhibited, the Wnt3a-stimulated interaction of β-catenin with Lef1, suggesting that OSTM1 may function as a mediator of this critical protein complex."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 18296023
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Norcantharidin reduces 80% of Wnt3a-induced β-catenin binding with lymphoid enhancer factor/T-cell factor protein [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26369691
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 4
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Strikingly, LiCl disrupted the SIRT1/LEF-1 complexes induced by glucose, most likely owing to competition for LEF-1 binding between β-catenin (accumulated by LiCl) and SIRT1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Collectively, the results indicate that combined glucose and LiCl treatment increases p300-LEF-1-β-catenin interaction while preventing SIRT1-LEF-1 and SIRT1-β-catenin interaction and suggest that increased acetylation by p300 may be required for nuclear retention of the LEF-1/β-catenin complex."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Interestingly, SIRT1 interaction with both β-catenin and LEF-1 is increased in response to high glucose, but these complexes are disrupted in the presence of LiCl."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Mechanistically, DBC1 stabilizes LEF1–β-catenin interaction by inhibiting SIRT1-mediated β-catenin deacetylation, thereby enhancing LEF1–β-catenin complex formation and long-range chromatin looping at the PROX1 locus."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 26477307
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 3
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The C-terminal TIEG1 expression construct primarily interacted with Lef1 and β-catenin in the nucleus (Figure xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28201653
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In addition to modulating β-catenin nuclear localization, we also reveal that TIEG1 interacts with Lef1 and β-catenin on Tcf/Lef enhancer elements resulting in enhancement of the canonical Wnt signaling pathway in osteoblasts."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28201653
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We also demonstrated that TIEG1 interacts with both Lef1 and β-catenin proteins suggesting that it associates with these factors as part of a larger transcriptional complex on Tcf/Lef enhancer elements encoded within classic canonical Wnt target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28201653
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 3
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The results are consistent with the general pathway in which Lef-1 forms a bipartite transcriptional activation complex with β-catenin ( Behrens et al., 1996; Willert and Nusse, 1998 )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12039047
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We also show that complementary charged and hydrophobic amino acids are required for formation of the bipartite beta-catenin-LEF-1 transcription factor."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 10966653
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Overexpression or constitutive activation of ILK has been shown to result in an invasive phenotype concomitant with downregulation of E-cadherin expression, translocation of β-catenin to the nucleus, and formation of the LEF-1–β-catenin bipartite complex ( xref ; xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 11402061
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 2
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Upon translocation to the nucleus, β-catenin interacts with T-cell factor 4 (TCF4) and lymphoid enhancer-binding factor 1 (LEF1) to regulate the expression of a wide range of genes at the transcriptional level xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28811522
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The nuclear β-catenin may bind in a complex with the T-cell factor 4 (Tcf4) to the Tcf4/ lymphoid enhancer factor (LEF) binding motif at the MDR1 promoter and thus enhancing promoter activity leading to increased efflux of anticancer drugs of the cell [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29409484
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 2
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "CHIP influences the interactions between β-catenin and LEF1 or GSK3β, as well as GSK3β and Arc."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33431799
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Taken together, CHIP acts as a cargo to regulate Wnt signaling and the degradation of Arc by influencing the interactions between β-catenin and LEF1 or GSK3β, as well as GSK3β and Arc."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33431799
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 2
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Menin is considered to support BMP-2- and Runx2-induced differentiation of mesenchymal cells into osteoblasts by interacting with Smad1/5, Runx2, β-catenin and LEF-1, although it has different effects on osteoblasts at later differentiation stages through TGF-β-Smad3 and AP-1 pathways."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22543820
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Conversely, a ternary complex composed of β-catenin::LEF1::Runx2 was found to inhibit Runx2 from binding to DNA in mouse osteoblast cells ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31740621
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 2
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We did observe strong binding of β-catenin, Tcf7 and Lef1 near Axin2 promoter and a distal site 30 kb upstream in high CHIR condition only."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33587034
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Β-catenin bound the transcription factors Tcf7 and Lef1, which promoted transcription of targeted genes, as evidenced by the increased gene expression of Jun , Fzd7 , and Nlk after TWS119 treatment."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31119051
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 2
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "AEG-1 forms a complex with LEF1 and β-catenin, and AEG-1-mediated activation of the Wnt/β-catenin pathway facilitated the maintenance of glioma stem-like cells and their self-renewal [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
PMC
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We showed that AEG-1 forms a complex with LEF1 and β-catenin, and AEG-1-mediated activation of Wnt/β-catenin pathway facilitated maintenance of glioma stem-like cells and their self-renewal ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 34353442
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 2
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The observation that β-catenin and CK2 alternate with TLE1 for binding to the CycD1 gene was confirmed by real-time PCR ChIP ( Figure 1 C) and indicates that LEF-1 cannot bind simultaneously to β-catenin and TLE1 in vivo, consistent with previous biochemical studies [10] ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17113388
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Since TLE1 binds antagonistically with β-catenin to DNA-bound Lef-1, decreasing affinity for TLE1 would also favor transcriptional activation."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21075118
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 2
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "LEF-1 normally binds with TCF-3 and β-catenin, forming transcriptional complexes that regulate the expression of cell fate genes [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29203441
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Β-catenin interactions with Lef1 and Tcf3 in the epidermis have been extensively characterised xref , xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 18213391
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 2
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Β-catenin and LEF1 together bind to the MITF promoter via the binding site on LEF-1 and promote MITF expression [ xref , xref , xref , xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 33332393
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "It has also been shown that β-catenin and Lef1 can directly interact with Mitf to induce Mitf-specific target genes in melanocytes xref , xref , indicating that Wnt/β-catenin signalling is important for maintenance of pigmented cells."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 24324671
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 2
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Interestingly, Nishita et al. recently demonstrated that Smad4 forms a complex with β-catenin and Lef1, which regulates the expression of genes required for the formation of the organizer in Xenopus [102] ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 12651222
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The two pathways were found to converge at the promoter of Xtwn , where Smad4, β-catenin, and Lef1 form a complex and bind to adjacent regulatory elements to co-regulate Xtwn expression."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19002158
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 2
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "We conclude that Krt15 and S1003A depend on β-catenin binding to ligand activated VDR and Lef1 for Wnt induction."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 18213391
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "A novel interaction between the VDR and LEF1 (lymphoid enhancer-binding factor-1) that is independent of β-catenin was identified."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21471213
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The overall structures of non phosphorylated Lef-1 (1-63) and phosphorylated Lef-1 (1-63) bound to beta-catenin are almost identical, except that residues 26-41 of Lef-1 are visible in the phosphorylated structure, where they form an extended loop and participate in both intra- and intermolecular contacts (XREF_FIG)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21075118
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Β-catenin synthesized in vitro bound strongly and specifically to GST-LEF1 ( xref C, upper panel), but GAC63 synthesized in vitro did not ( xref C, lower panel)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17344318
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "For example, β-catenin enhanced interactions between Lef1 (GAL4-Lef1) and GRIP-1 (VP-GRIP), and this was primarily due to coactivation and interactions of β-catenin with Lef1 but not GRIP-1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21169420
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The downstream effects of TGF-β3 signaling causes LEF-1 gene transcription which then associates with β-catenin and results in either suppression or repression of EMT genes as described above. xref Thus, a complex interplay of the three isoforms is involved in EMT."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 22561306
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
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+
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "36 These data show that Wnt signaling, specifically through β-catenin, enhances NR4A2 activity and, conversely, that NR4A2 influences β-catenin activity through direct physical interaction of NR4A2 with β-catenin and LEF1."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 19549529
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Β-catenin did not bind to the GAPDH promoter that did not have the LEF-1 binding sites."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23396279
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
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+
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+
+
+
+
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+
+
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+
+
+
+
+
+
+
+
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Treatment of hESCs with Wnt3a induces binding of β-catenin and cohesin to LEF-1 sites and directs enhancer looping to nearby ME target gene promoters, activating transcription in a cohesin-dependent manner."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 25936800
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
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+
+
+
+
+
+
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+
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Endogenous HPIP or β-catenin associated with endogenous LEF1 in the nucleus (Supplementary Fig. xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 30659184
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Collectively, the results indicate that combined glucose and LiCl treatment increases p300-LEF-1-β-catenin interaction while preventing SIRT1-LEF-1 and SIRT1-β-catenin interaction and suggest that increased acetylation by p300 may be required for nuclear retention of the LEF-1/β-catenin complex."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23273980
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
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+
+
+
+
+
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+
+
+
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+
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+
+
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The expression of perivenous genes was also associated with the interaction of β-catenin with LEF1 and hepatocyte nuclear factor 4-α (HNF4α) that could also be detected with higher levels in the perivenous area xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 28126520
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The nuclear pSmad2/3 can directly associate with the lymphoid enhancer binding factor 1/T cell-specific factor (LEF1/TCF) to synergistically activate β-catenin target genes."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 27479195
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
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+
+
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+
+
+
+
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 1
+
+
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "A chromatin immunoprecipitation (ChIP) assay with an anti–β-catenin or an anti–LEF-1 antibody showed that treatment of U87/EGFR cells with Wnt3A, EGF, or expression of CA β-catenin increased the binding of β-catenin and LEF-1 to the promoter region of CD274 ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32860047
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
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+
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+
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+
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+
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+
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This hypothesis agrees with the competition in vitro between E-cadherin cytosolic tail and Lef1 for binding to β-catenin 35 leading to the suppression of Lef1-mediated transactivation in SW480 cells."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 20813963
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In String database, PITX2 was predicted to interact with LEF1, CTNNB1 and AXIN, all of which are the key factors of Wnt/β-catenin signaling pathway (Fig. xref c)."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 31043858
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
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+
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+
+
+
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+
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+
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+
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+
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+
+
+
+
+
+
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+
+
+
+
+
+
+
+
+
+
+
+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 1
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+
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "40,42,47–49
+ Moreover, it has been shown that the LEF-1-β-catenin complex binds in vitro to the E-cadherin promoter to regulate the transcription of this gene, suggesting that although adhesion function and intracellular signaling activity of β-catenin are independent, they could be interrelated via negative regulation by E-cadherin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 9811344
+
+
+
+
+
+
+
+
+
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+
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+
+
+
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+ |
+
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+
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
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+ 1
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+
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Loss of APC is associated with the accumulation of β-Catenin in the nucleus, which activates the T-cell factor (TCF) and the lymphoid enhancer factor (LEF) transcription factor as targets of the canonical Wnt pathway xref , xref ."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 29549256
+
+
+
+
+
+
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+
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+
+
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+ |
+
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+
+
+
+
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+
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+
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+
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+
+
+
+
+
+
+ 1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The full-length Tcf7 could efficiently bind to Gro2 ( xref ), β-catenin ( xref ), and Lef1 ( xref ), whereas the myc-Tcf7_ΔGroBD, myc-Tcf7_ΔN, and myc-Tcf7_ΔHMG could not bind to Gro2, β-catenin, and Lef1, respectively ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 32702011
+
+
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+ |
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+
+
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+
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+ 1
+
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "This opens the possibility that β-catenin and PRMT6 could simultaneously interact with LEF1."
+
+
+
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+
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+
+
+
+
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+ 34001852
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+ |
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+
+
+
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+
+
+ 1
+
+
+
+
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The N-termini of XTcf-3 and LEF-1 interact with the Armadillo repeat region of β-catenin ( Behrens et al. 1996; Huber et al. 1996; Molenaar et al. 1996 )."
+
+
+
+
+
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+
+
+
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+
+
+ 9118222
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+ |
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+
+
+
+
+
+
+ 1
+
+
+
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "To compare the interaction between these Rac1-specific mutants of β-catenin and LEF-1, a PLA was used."
+
+
+
+
+
+
+
+
+
+
+
+
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+ 26403202
+
+
+
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+ |
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+
+
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+
+
+ 1
+
+
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+
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+
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Concurrently, we designed peptides for targeting the intracellular domain of EpCAM ( xref ), which interacts with β-catenin and Lef1 to form a nuclear complex that regulates β-catenin-dependent transcription ( xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21256838
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+ |
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+
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+ 1
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "While beta-catenin can bind directly to both AR and Lef1, GRIP1 can only bind directly to AR; the ability of GRIP1 to associate with and function as a coactivator for Lef1 is entirely dependent on the presence of beta-catenin."
+
+
+
+
+
+
+
+
+
+
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+
+
+ 14638683
+
+
+
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+ 1
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+ |
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+
+
+
+
+
+ No evidence text available
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 15684397
+
+
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+ |
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+
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+
+
+
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+
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+
+
+
+
+
+
+
+ 1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Transcriptional responses induced by Wnt depend on the association of either beta-catenin or gamma-catenin with lymphoid enhancer factor/T cell factor transcription factors."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 17906078
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+
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+
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+ |
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+
+
+
+
+
+
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+
+
+
+
+
+
+
+
+
+
+
+
+ 1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "The EpCAM intracellular domain (EpICD) peptide translocates to the cell nucleus following proteolytic cleavage to assemble a transcriptional activation complex with LIM domains, β-catenin, Lef-1, and other transcription factors to activate Wnt target gene expression [ xref ]."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 30467381
+
+
+
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+
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+
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+ |
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "Research by Juan Galceran et al. [ xref ] confirmed that Lef1 binds to β-catenin in the nucleus to form the Lef1-β-catenin complex that activates the expression of endogenous Dll1 in fibroblasts."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 23602012
+
+
+
+
+
+
+
+
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+
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+
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+
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+
+
+
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+
+
+
+
+
+
+
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+
+
+ |
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "In pull-down assays, HINT1 did not bind to β-catenin or LEF-1 nor did it impair the LEF-1/β-catenin interaction or the binding of pontin or reptin to β-catenin."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21316334
+
+
+
+
+
+
+
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+
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+
+
+
+
+
+
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+
+ |
+
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+ "PitX2 also regulates the canonical Wnt signaling system through interactions with beta-catenin, dkk2 and Lef1, which in turn feedback onto PitX2 expression ( xref ; xref )."
+
+
+
+
+
+
+
+
+
+
+
+
+
+ 21309065
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+