diff --git a/deno_dist/utils/body.ts b/deno_dist/utils/body.ts
index cf9a2b62b..db47b8c23 100644
--- a/deno_dist/utils/body.ts
+++ b/deno_dist/utils/body.ts
@@ -1,6 +1,7 @@
 import { HonoRequest } from '../request.ts'
 
-export type BodyData = Record<string, string | File | (string | File)[]>
+type BodyDataValue = string | File | (string | File)[] | { [key: string]: BodyDataValue }
+export type BodyData = Record<string, BodyDataValue>
 export type ParseBodyOptions = {
   /**
    * Determines whether all fields with multiple values should be parsed as arrays.
@@ -17,13 +18,38 @@ export type ParseBodyOptions = {
    * If all is true:
    * parseBody should return { file: ['aaa', 'bbb'], message: 'hello' }
    */
-  all?: boolean
+  all: boolean
+  /**
+   * Determines whether all fields with dot notation should be parsed as nested objects.
+   * @default false
+   * @example
+   * const data = new FormData()
+   * data.append('obj.key1', 'value1')
+   * data.append('obj.key2', 'value2')
+   *
+   * If dot is false:
+   * parseBody should return { 'obj.key1': 'value1', 'obj.key2': 'value2' }
+   *
+   * If dot is true:
+   * parseBody should return { obj: { key1: 'value1', key2: 'value2' } }
+   */
+  dot: boolean
 }
 
+/**
+ * Parses the body of a request based on the provided options.
+ *
+ * @template T - The type of the parsed body data.
+ * @param {HonoRequest | Request} request - The request object to parse.
+ * @param {Partial<ParseBodyOptions>} [options] - Options for parsing the body.
+ * @returns {Promise<T>} The parsed body data.
+ */
 export const parseBody = async <T extends BodyData = BodyData>(
   request: HonoRequest | Request,
-  options: ParseBodyOptions = { all: false }
+  options: Partial<ParseBodyOptions> = Object.create(null)
 ): Promise<T> => {
+  const { all = false, dot = false } = options
+
   const headers = request instanceof HonoRequest ? request.raw.headers : request.headers
   const contentType = headers.get('Content-Type')
 
@@ -31,12 +57,20 @@ export const parseBody = async <T extends BodyData = BodyData>(
     (contentType !== null && contentType.startsWith('multipart/form-data')) ||
     (contentType !== null && contentType.startsWith('application/x-www-form-urlencoded'))
   ) {
-    return parseFormData<T>(request, options)
+    return parseFormData<T>(request, { all, dot })
   }
 
   return {} as T
 }
 
+/**
+ * Parses form data from a request.
+ *
+ * @template T - The type of the parsed body data.
+ * @param {HonoRequest | Request} request - The request object containing form data.
+ * @param {ParseBodyOptions} options - Options for parsing the form data.
+ * @returns {Promise<T>} The parsed body data.
+ */
 async function parseFormData<T extends BodyData = BodyData>(
   request: HonoRequest | Request,
   options: ParseBodyOptions
@@ -50,33 +84,92 @@ async function parseFormData<T extends BodyData = BodyData>(
   return {} as T
 }
 
+/**
+ * Converts form data to body data based on the provided options.
+ *
+ * @template T - The type of the parsed body data.
+ * @param {FormData} formData - The form data to convert.
+ * @param {ParseBodyOptions} options - Options for parsing the form data.
+ * @returns {T} The converted body data.
+ */
 function convertFormDataToBodyData<T extends BodyData = BodyData>(
   formData: FormData,
   options: ParseBodyOptions
 ): T {
-  const form: BodyData = {}
+  const form: BodyData = Object.create(null)
 
   formData.forEach((value, key) => {
     const shouldParseAllValues = options.all || key.endsWith('[]')
 
-    if (!shouldParseAllValues) {
-      form[key] = value
-    } else {
+    if (shouldParseAllValues) {
       handleParsingAllValues(form, key, value)
+    } else {
+      form[key] = value
     }
   })
 
+  if (options.dot) {
+    const nestedForm: BodyData = Object.create(null)
+
+    Object.entries(form).forEach(([key, value]) => {
+      const shouldParseDotValues = key.includes('.')
+
+      if (shouldParseDotValues) {
+        handleParsingNestedValues(nestedForm, key, value)
+      } else {
+        nestedForm[key] = value
+      }
+    })
+
+    return nestedForm as T
+  }
+
   return form as T
 }
 
+/**
+ * Handles parsing all values for a given key, supporting multiple values as arrays.
+ *
+ * @param {BodyData} form - The form data object.
+ * @param {string} key - The key to parse.
+ * @param {FormDataEntryValue} value - The value to assign.
+ */
 const handleParsingAllValues = (form: BodyData, key: string, value: FormDataEntryValue): void => {
-  const formKey = form[key]
-
-  if (formKey && Array.isArray(formKey)) {
-    ;(form[key] as (string | File)[]).push(value)
-  } else if (formKey) {
-    form[key] = [formKey, value]
+  if (form[key] !== undefined) {
+    if (Array.isArray(form[key])) {
+      ;(form[key] as (string | File)[]).push(value)
+    } else {
+      form[key] = [form[key] as string | File, value]
+    }
   } else {
     form[key] = value
   }
 }
+
+/**
+ * Handles parsing nested values using dot notation keys.
+ *
+ * @param {BodyData} form - The form data object.
+ * @param {string} key - The dot notation key.
+ * @param {BodyDataValue} value - The value to assign.
+ */
+const handleParsingNestedValues = (form: BodyData, key: string, value: BodyDataValue): void => {
+  let nestedForm = form
+  const keys = key.split('.')
+
+  keys.forEach((key, index) => {
+    if (index === keys.length - 1) {
+      nestedForm[key] = value
+    } else {
+      if (
+        !nestedForm[key] ||
+        typeof nestedForm[key] !== 'object' ||
+        Array.isArray(nestedForm[key]) ||
+        nestedForm[key] instanceof File
+      ) {
+        nestedForm[key] = Object.create(null)
+      }
+      nestedForm = nestedForm[key] as BodyData
+    }
+  })
+}
diff --git a/src/utils/body.test.ts b/src/utils/body.test.ts
index 80443466b..d04ed0801 100644
--- a/src/utils/body.test.ts
+++ b/src/utils/body.test.ts
@@ -50,6 +50,46 @@ describe('Parse Body Util', () => {
     })
   })
 
+  it('should not update file object properties', async () => {
+    const file = new File(['foo'], 'file1', {
+      type: 'application/octet-stream',
+    })
+    const data = new FormData()
+
+    const req = createRequest(FORM_URL, 'POST', data)
+    vi.spyOn(req, 'formData').mockImplementation(
+      async () =>
+        ({
+          forEach: (cb) => {
+            cb(file, 'file', data)
+            cb('hoo', 'file.hoo', data)
+          },
+        } as FormData)
+    )
+
+    const parsedData = await parseBody(req, { dot: true })
+    expect(parsedData.file).not.instanceOf(File)
+    expect(parsedData).toEqual({
+      file: {
+        hoo: 'hoo',
+      },
+    })
+  })
+
+  it('should override value if `all` option is false', async () => {
+    const data = new FormData()
+    data.append('file', 'aaa')
+    data.append('file', 'bbb')
+    data.append('message', 'hello')
+
+    const req = createRequest(FORM_URL, 'POST', data)
+
+    expect(await parseBody(req)).toEqual({
+      file: 'bbb',
+      message: 'hello',
+    })
+  })
+
   it('should parse multiple values if `all` option is true', async () => {
     const data = new FormData()
     data.append('file', 'aaa')
@@ -64,6 +104,101 @@ describe('Parse Body Util', () => {
     })
   })
 
+  it('should not parse nested values in default', async () => {
+    const data = new FormData()
+    data.append('obj.key1', 'value1')
+    data.append('obj.key2', 'value2')
+
+    const req = createRequest(FORM_URL, 'POST', data)
+
+    expect(await parseBody(req, { dot: false })).toEqual({
+      'obj.key1': 'value1',
+      'obj.key2': 'value2',
+    })
+  })
+
+  it('should not parse nested values in default for non-nested keys', async () => {
+    const data = new FormData()
+    data.append('key1', 'value1')
+    data.append('key2', 'value2')
+
+    const req = createRequest(FORM_URL, 'POST', data)
+
+    expect(await parseBody(req, { dot: false })).toEqual({
+      key1: 'value1',
+      key2: 'value2',
+    })
+  })
+
+  it('should handle nested values and non-nested values together with dot option true', async () => {
+    const data = new FormData()
+    data.append('obj.key1', 'value1')
+    data.append('obj.key2', 'value2')
+    data.append('key3', 'value3')
+
+    const req = createRequest(FORM_URL, 'POST', data)
+
+    expect(await parseBody(req, { dot: true })).toEqual({
+      obj: { key1: 'value1', key2: 'value2' },
+      key3: 'value3',
+    })
+  })
+
+  it('should handle deeply nested objects with dot option true', async () => {
+    const data = new FormData()
+    data.append('a.b.c.d', 'value')
+
+    const req = createRequest(FORM_URL, 'POST', data)
+
+    expect(await parseBody(req, { dot: true })).toEqual({
+      a: { b: { c: { d: 'value' } } },
+    })
+  })
+
+  it('should parse nested values if `dot` option is true', async () => {
+    const data = new FormData()
+    data.append('obj.key1', 'value1')
+    data.append('obj.key2', 'value2')
+
+    const req = createRequest(FORM_URL, 'POST', data)
+
+    expect(await parseBody(req, { dot: true })).toEqual({
+      obj: { key1: 'value1', key2: 'value2' },
+    })
+  })
+
+  it('should parse data if both `all` and `dot` are set', async () => {
+    const data = new FormData()
+    data.append('obj.sub.foo', 'value1')
+    data.append('obj.sub.foo', 'value2')
+    data.append('key', 'value3')
+
+    const req = createRequest(FORM_URL, 'POST', data)
+
+    expect(await parseBody(req, { dot: true, all: true })).toEqual({
+      obj: { sub: { foo: ['value1', 'value2'] } },
+      key: 'value3',
+    })
+  })
+
+  it('should parse nested values if values are `File`', async () => {
+    const file1 = new File(['foo'], 'file1', {
+      type: 'application/octet-stream',
+    })
+    const file2 = new File(['bar'], 'file2', {
+      type: 'application/octet-stream',
+    })
+    const data = new FormData()
+    data.append('file.file1', file1)
+    data.append('file.file2', file2)
+
+    const req = createRequest(FORM_URL, 'POST', data)
+
+    expect(await parseBody(req, { all: true, dot: true })).toEqual({
+      file: { file1, file2 },
+    })
+  })
+
   it('should parse multiple values if values are `File`', async () => {
     const file1 = new File(['foo'], 'file1', {
       type: 'application/octet-stream',
diff --git a/src/utils/body.ts b/src/utils/body.ts
index 295b807d4..8013dd212 100644
--- a/src/utils/body.ts
+++ b/src/utils/body.ts
@@ -1,6 +1,7 @@
 import { HonoRequest } from '../request'
 
-export type BodyData = Record<string, string | File | (string | File)[]>
+type BodyDataValue = string | File | (string | File)[] | { [key: string]: BodyDataValue }
+export type BodyData = Record<string, BodyDataValue>
 export type ParseBodyOptions = {
   /**
    * Determines whether all fields with multiple values should be parsed as arrays.
@@ -17,13 +18,38 @@ export type ParseBodyOptions = {
    * If all is true:
    * parseBody should return { file: ['aaa', 'bbb'], message: 'hello' }
    */
-  all?: boolean
+  all: boolean
+  /**
+   * Determines whether all fields with dot notation should be parsed as nested objects.
+   * @default false
+   * @example
+   * const data = new FormData()
+   * data.append('obj.key1', 'value1')
+   * data.append('obj.key2', 'value2')
+   *
+   * If dot is false:
+   * parseBody should return { 'obj.key1': 'value1', 'obj.key2': 'value2' }
+   *
+   * If dot is true:
+   * parseBody should return { obj: { key1: 'value1', key2: 'value2' } }
+   */
+  dot: boolean
 }
 
+/**
+ * Parses the body of a request based on the provided options.
+ *
+ * @template T - The type of the parsed body data.
+ * @param {HonoRequest | Request} request - The request object to parse.
+ * @param {Partial<ParseBodyOptions>} [options] - Options for parsing the body.
+ * @returns {Promise<T>} The parsed body data.
+ */
 export const parseBody = async <T extends BodyData = BodyData>(
   request: HonoRequest | Request,
-  options: ParseBodyOptions = { all: false }
+  options: Partial<ParseBodyOptions> = Object.create(null)
 ): Promise<T> => {
+  const { all = false, dot = false } = options
+
   const headers = request instanceof HonoRequest ? request.raw.headers : request.headers
   const contentType = headers.get('Content-Type')
 
@@ -31,12 +57,20 @@ export const parseBody = async <T extends BodyData = BodyData>(
     (contentType !== null && contentType.startsWith('multipart/form-data')) ||
     (contentType !== null && contentType.startsWith('application/x-www-form-urlencoded'))
   ) {
-    return parseFormData<T>(request, options)
+    return parseFormData<T>(request, { all, dot })
   }
 
   return {} as T
 }
 
+/**
+ * Parses form data from a request.
+ *
+ * @template T - The type of the parsed body data.
+ * @param {HonoRequest | Request} request - The request object containing form data.
+ * @param {ParseBodyOptions} options - Options for parsing the form data.
+ * @returns {Promise<T>} The parsed body data.
+ */
 async function parseFormData<T extends BodyData = BodyData>(
   request: HonoRequest | Request,
   options: ParseBodyOptions
@@ -50,33 +84,92 @@ async function parseFormData<T extends BodyData = BodyData>(
   return {} as T
 }
 
+/**
+ * Converts form data to body data based on the provided options.
+ *
+ * @template T - The type of the parsed body data.
+ * @param {FormData} formData - The form data to convert.
+ * @param {ParseBodyOptions} options - Options for parsing the form data.
+ * @returns {T} The converted body data.
+ */
 function convertFormDataToBodyData<T extends BodyData = BodyData>(
   formData: FormData,
   options: ParseBodyOptions
 ): T {
-  const form: BodyData = {}
+  const form: BodyData = Object.create(null)
 
   formData.forEach((value, key) => {
     const shouldParseAllValues = options.all || key.endsWith('[]')
 
-    if (!shouldParseAllValues) {
-      form[key] = value
-    } else {
+    if (shouldParseAllValues) {
       handleParsingAllValues(form, key, value)
+    } else {
+      form[key] = value
     }
   })
 
+  if (options.dot) {
+    const nestedForm: BodyData = Object.create(null)
+
+    Object.entries(form).forEach(([key, value]) => {
+      const shouldParseDotValues = key.includes('.')
+
+      if (shouldParseDotValues) {
+        handleParsingNestedValues(nestedForm, key, value)
+      } else {
+        nestedForm[key] = value
+      }
+    })
+
+    return nestedForm as T
+  }
+
   return form as T
 }
 
+/**
+ * Handles parsing all values for a given key, supporting multiple values as arrays.
+ *
+ * @param {BodyData} form - The form data object.
+ * @param {string} key - The key to parse.
+ * @param {FormDataEntryValue} value - The value to assign.
+ */
 const handleParsingAllValues = (form: BodyData, key: string, value: FormDataEntryValue): void => {
-  const formKey = form[key]
-
-  if (formKey && Array.isArray(formKey)) {
-    ;(form[key] as (string | File)[]).push(value)
-  } else if (formKey) {
-    form[key] = [formKey, value]
+  if (form[key] !== undefined) {
+    if (Array.isArray(form[key])) {
+      ;(form[key] as (string | File)[]).push(value)
+    } else {
+      form[key] = [form[key] as string | File, value]
+    }
   } else {
     form[key] = value
   }
 }
+
+/**
+ * Handles parsing nested values using dot notation keys.
+ *
+ * @param {BodyData} form - The form data object.
+ * @param {string} key - The dot notation key.
+ * @param {BodyDataValue} value - The value to assign.
+ */
+const handleParsingNestedValues = (form: BodyData, key: string, value: BodyDataValue): void => {
+  let nestedForm = form
+  const keys = key.split('.')
+
+  keys.forEach((key, index) => {
+    if (index === keys.length - 1) {
+      nestedForm[key] = value
+    } else {
+      if (
+        !nestedForm[key] ||
+        typeof nestedForm[key] !== 'object' ||
+        Array.isArray(nestedForm[key]) ||
+        nestedForm[key] instanceof File
+      ) {
+        nestedForm[key] = Object.create(null)
+      }
+      nestedForm = nestedForm[key] as BodyData
+    }
+  })
+}