diff --git a/package.json b/package.json
index 8240ab8..9b26d90 100644
--- a/package.json
+++ b/package.json
@@ -8,6 +8,7 @@
     "test:unit": "vitest --run test/unit",
     "test:integration": "bun test:integration:api && bun test:integration:hono-jsx",
     "test:integration:hono-jsx": "vitest run -c ./test/hono-jsx/vitest.config.ts ./test/hono-jsx/integration.test.ts",
+    "test:integration:hono-jsx:watch": "vitest -c ./test/hono-jsx/vitest.config.ts ./test/hono-jsx/integration.test.ts",
     "test:integration:api": "vitest run -c ./test/api/vitest.config.ts ./test/api/integration.test.ts",
     "test:e2e": "playwright test -c ./test/hono-jsx/playwright.config.ts ./test/hono-jsx/e2e.test.ts",
     "typecheck": "tsc --noEmit",
@@ -117,4 +118,4 @@
   "optionalDependencies": {
     "@rollup/rollup-linux-x64-gnu": "^4.9.6"
   }
-}
+}
\ No newline at end of file
diff --git a/src/server/server.ts b/src/server/server.ts
index 79d6f6b..b183183 100644
--- a/src/server/server.ts
+++ b/src/server/server.ts
@@ -74,10 +74,13 @@ export const createApp = <E extends Env>(options?: ServerOptions<E>): Hono<E> =>
       eager: true,
     })
   const rendererList = listByDirectory(RENDERER_FILE)
-  const applyRenderer = (rendererFile: string) => {
+
+  const applyRenderer = (app: Hono, rendererFile: string) => {
     const renderer = RENDERER_FILE[rendererFile]
-    const path = pathToDirectoryPath(rendererFile).replace(rootRegExp, '')
-    app.all(`${filePathToPath(path)}*`, renderer.default)
+    const rendererDefault = renderer['default']
+    if (rendererDefault) {
+      app.all('*', rendererDefault)
+    }
   }
 
   // Routes
@@ -94,30 +97,26 @@ export const createApp = <E extends Env>(options?: ServerOptions<E>): Hono<E> =>
       const subApp = new Hono()
 
       // Renderer
-      let rendererFiles = rendererList[dir]
-
-      if (rendererFiles) {
-        applyRenderer(rendererFiles[0])
-      }
-
-      if (!rendererFiles) {
-        const dirPaths = dir.split('/')
-        const getRendererPaths = (paths: string[]) => {
-          rendererFiles = rendererList[paths.join('/')]
-          if (!rendererFiles) {
-            paths.pop()
-            if (paths.length) {
-              getRendererPaths(paths)
-            }
+      let rendererPaths = rendererList[dir] ?? []
+
+      const getRendererPaths = (paths: string[]) => {
+        rendererPaths = rendererList[paths.join('/')]
+        if (!rendererPaths) {
+          paths.pop()
+          if (paths.length) {
+            getRendererPaths(paths)
           }
-          return rendererFiles
-        }
-        rendererFiles = getRendererPaths(dirPaths)
-        if (rendererFiles) {
-          applyRenderer(rendererFiles[0])
         }
+        return rendererPaths ?? []
       }
 
+      const dirPaths = dir.split('/')
+      rendererPaths = getRendererPaths(dirPaths)
+      rendererPaths.sort((a, b) => a.split('/').length - b.split('/').length)
+      rendererPaths.map((path) => {
+        applyRenderer(subApp, path)
+      })
+
       // Root path
       let rootPath = dir.replace(rootRegExp, '')
       rootPath = filePathToPath(rootPath)
diff --git a/test/hono-jsx/app-nested/routes/nested/_renderer.tsx b/test/hono-jsx/app-nested/routes/nested/_renderer.tsx
new file mode 100644
index 0000000..5e4bec3
--- /dev/null
+++ b/test/hono-jsx/app-nested/routes/nested/_renderer.tsx
@@ -0,0 +1,14 @@
+import { jsxRenderer } from 'hono/jsx-renderer'
+
+export default jsxRenderer(
+  ({ children }) => {
+    return (
+      <main>
+        <>{children}</>
+      </main>
+    )
+  },
+  {
+    docType: false,
+  }
+)
diff --git a/test/hono-jsx/app-nested/routes/nested/foo/_renderer.tsx b/test/hono-jsx/app-nested/routes/nested/foo/_renderer.tsx
new file mode 100644
index 0000000..714c237
--- /dev/null
+++ b/test/hono-jsx/app-nested/routes/nested/foo/_renderer.tsx
@@ -0,0 +1,17 @@
+import { jsxRenderer } from 'hono/jsx-renderer'
+
+export default jsxRenderer(
+  ({ children, Layout }) => {
+    return (
+      <Layout>
+        <>
+          <nav>foo menu</nav>
+          {children}
+        </>
+      </Layout>
+    )
+  },
+  {
+    docType: false,
+  }
+)
diff --git a/test/hono-jsx/app-nested/routes/nested/foo/bar/_renderer.tsx b/test/hono-jsx/app-nested/routes/nested/foo/bar/_renderer.tsx
new file mode 100644
index 0000000..a6694df
--- /dev/null
+++ b/test/hono-jsx/app-nested/routes/nested/foo/bar/_renderer.tsx
@@ -0,0 +1,17 @@
+import { jsxRenderer } from 'hono/jsx-renderer'
+
+export default jsxRenderer(
+  ({ children, Layout }) => {
+    return (
+      <Layout>
+        <>
+          <nav>bar menu</nav>
+          {children}
+        </>
+      </Layout>
+    )
+  },
+  {
+    docType: false,
+  }
+)
diff --git a/test/hono-jsx/app-nested/routes/nested/foo/bar/baz/index.tsx b/test/hono-jsx/app-nested/routes/nested/foo/bar/baz/index.tsx
new file mode 100644
index 0000000..bff8a11
--- /dev/null
+++ b/test/hono-jsx/app-nested/routes/nested/foo/bar/baz/index.tsx
@@ -0,0 +1,3 @@
+export default function Baz() {
+  return <h1>Baz</h1>
+}
diff --git a/test/hono-jsx/app-nested/routes/nested/foo/bar/index.tsx b/test/hono-jsx/app-nested/routes/nested/foo/bar/index.tsx
new file mode 100644
index 0000000..aa34810
--- /dev/null
+++ b/test/hono-jsx/app-nested/routes/nested/foo/bar/index.tsx
@@ -0,0 +1,3 @@
+export default function Bar() {
+  return <h1>Bar</h1>
+}
diff --git a/test/hono-jsx/app-nested/routes/nested/foo/index.tsx b/test/hono-jsx/app-nested/routes/nested/foo/index.tsx
new file mode 100644
index 0000000..278b227
--- /dev/null
+++ b/test/hono-jsx/app-nested/routes/nested/foo/index.tsx
@@ -0,0 +1,3 @@
+export default function Foo() {
+  return <h1>Foo</h1>
+}
diff --git a/test/hono-jsx/app-nested/routes/nested/index.tsx b/test/hono-jsx/app-nested/routes/nested/index.tsx
new file mode 100644
index 0000000..14900d1
--- /dev/null
+++ b/test/hono-jsx/app-nested/routes/nested/index.tsx
@@ -0,0 +1,3 @@
+export default function Nested() {
+  return <h1>Nested</h1>
+}
diff --git a/test/hono-jsx/integration.test.ts b/test/hono-jsx/integration.test.ts
index fdb1122..cf64130 100644
--- a/test/hono-jsx/integration.test.ts
+++ b/test/hono-jsx/integration.test.ts
@@ -132,110 +132,150 @@ describe('Basic', () => {
     expect(res.status).toBe(500)
     expect(await res.text()).toBe('Internal Server Error')
   })
+})
 
-  describe('With preserved', () => {
-    const ROUTES = import.meta.glob('./app/routes/**/[a-z[-][a-z-_[]*.(tsx|ts)', {
-      eager: true,
-    })
+describe('With preserved', () => {
+  const ROUTES = import.meta.glob('./app/routes/**/[a-z[-][a-z-_[]*.(tsx|ts)', {
+    eager: true,
+  })
 
-    const RENDERER = import.meta.glob('./app/routes/**/_renderer.tsx', {
-      eager: true,
-    })
+  const RENDERER = import.meta.glob('./app/routes/**/_renderer.tsx', {
+    eager: true,
+  })
 
-    const NOT_FOUND = import.meta.glob('./app/routes/_404.tsx', {
-      eager: true,
-    })
+  const NOT_FOUND = import.meta.glob('./app/routes/_404.tsx', {
+    eager: true,
+  })
 
-    const ERROR = import.meta.glob('./app/routes/_error.tsx', {
-      eager: true,
-    })
+  const ERROR = import.meta.glob('./app/routes/_error.tsx', {
+    eager: true,
+  })
 
-    const app = createApp({
-      root: './app/routes',
-      ROUTES: ROUTES as any,
-      RENDERER: RENDERER as any,
-      NOT_FOUND: NOT_FOUND as any,
-      ERROR: ERROR as any,
-    })
+  const app = createApp({
+    root: './app/routes',
+    ROUTES: ROUTES as any,
+    RENDERER: RENDERER as any,
+    NOT_FOUND: NOT_FOUND as any,
+    ERROR: ERROR as any,
+  })
 
-    it('Should return 200 response - /', async () => {
-      const res = await app.request('/')
-      expect(res.status).toBe(200)
-      expect(await res.text()).toBe(
-        '<!DOCTYPE html><html><head><title>This is a title</title></head><body><h1>Hello</h1></body></html>'
-      )
-    })
+  it('Should return 200 response - /', async () => {
+    const res = await app.request('/')
+    expect(res.status).toBe(200)
+    expect(await res.text()).toBe(
+      '<!DOCTYPE html><html><head><title>This is a title</title></head><body><h1>Hello</h1></body></html>'
+    )
+  })
 
-    it('Should return 404 response - /foo', async () => {
-      const res = await app.request('/foo')
-      expect(res.status).toBe(404)
-      expect(await res.text()).toBe(
-        '<!DOCTYPE html><html><head><title>Not Found</title></head><body><h1>Not Found</h1></body></html>'
-      )
-    })
+  it('Should return 404 response - /foo', async () => {
+    const res = await app.request('/foo')
+    expect(res.status).toBe(404)
+    expect(await res.text()).toBe(
+      '<!DOCTYPE html><html><head><title>Not Found</title></head><body><h1>Not Found</h1></body></html>'
+    )
+  })
 
-    it('Should return 200 response /about/me', async () => {
-      const res = await app.request('/about/me')
-      expect(res.status).toBe(200)
-      // hono/jsx escape a single quote to &#39;
-      expect(await res.text()).toBe(
-        '<!DOCTYPE html><html><head><title>me</title></head><body><p>It&#39;s me</p><b>My name is me</b></body></html>'
-      )
-    })
+  it('Should return 200 response /about/me', async () => {
+    const res = await app.request('/about/me')
+    expect(res.status).toBe(200)
+    // hono/jsx escape a single quote to &#39;
+    expect(await res.text()).toBe(
+      '<!DOCTYPE html><html><head><title>me</title></head><body><p>It&#39;s me</p><b>My name is me</b></body></html>'
+    )
+  })
 
-    it('Should return 200 response /about/me/address', async () => {
-      const res = await app.request('/about/me/address')
-      expect(res.status).toBe(200)
-      // hono/jsx escape a single quote to &#39;
-      expect(await res.text()).toBe(
-        '<!DOCTYPE html><html><head><title>me&#39;s address</title></head><body><h1>About</h1><address><b>me&#39;s address</b></address></body></html>'
-      )
-    })
+  it('Should return 200 response /about/me/address', async () => {
+    const res = await app.request('/about/me/address')
+    expect(res.status).toBe(200)
+    // hono/jsx escape a single quote to &#39;
+    expect(await res.text()).toBe(
+      '<!DOCTYPE html><html><head><title>me&#39;s address</title></head><body><h1>About</h1><address><b>me&#39;s address</b></address></body></html>'
+    )
+  })
 
-    it('Should return 200 response /interaction', async () => {
-      const res = await app.request('/interaction')
-      expect(res.status).toBe(200)
-      // hono/jsx escape a single quote to &#39;
-      expect(await res.text()).toBe(
-        '<!DOCTYPE html><html><head><title></title><script type="module" src="/app/client.ts"></script></head><body><honox-island component-name="Counter.tsx" data-serialized-props="{&quot;initial&quot;:5}"><div><p>Count: 5</p><button onClick="() =&gt; setCount(count + 1)">Increment</button></div></honox-island></body></html>'
-      )
-    })
+  it('Should return 200 response /interaction', async () => {
+    const res = await app.request('/interaction')
+    expect(res.status).toBe(200)
+    // hono/jsx escape a single quote to &#39;
+    expect(await res.text()).toBe(
+      '<!DOCTYPE html><html><head><title></title><script type="module" src="/app/client.ts"></script></head><body><honox-island component-name="Counter.tsx" data-serialized-props="{&quot;initial&quot;:5}"><div><p>Count: 5</p><button onClick="() =&gt; setCount(count + 1)">Increment</button></div></honox-island></body></html>'
+    )
+  })
 
-    it('Should return 500 response /throw_error', async () => {
-      const res = await app.request('/throw_error')
-      expect(res.status).toBe(500)
-      expect(await res.text()).toBe(
-        '<!DOCTYPE html><html><head><title>Internal Server Error</title></head><body><h1>Custom Error Message: Foo</h1></body></html>'
-      )
-    })
+  it('Should return 500 response /throw_error', async () => {
+    const res = await app.request('/throw_error')
+    expect(res.status).toBe(500)
+    expect(await res.text()).toBe(
+      '<!DOCTYPE html><html><head><title>Internal Server Error</title></head><body><h1>Custom Error Message: Foo</h1></body></html>'
+    )
   })
+})
 
-  describe('API', () => {
-    const ROUES = import.meta.glob('./app/routes//**/[a-z[-][a-z-_[]*.(tsx|ts)', {
-      eager: true,
-    })
+describe('API', () => {
+  const ROUES = import.meta.glob('./app/routes//**/[a-z[-][a-z-_[]*.(tsx|ts)', {
+    eager: true,
+  })
 
-    const app = createApp({
-      root: './app/routes',
-      ROUTES: ROUES as any,
-    })
+  const app = createApp({
+    root: './app/routes',
+    ROUTES: ROUES as any,
+  })
 
-    it('Should return 200 response - /api', async () => {
-      const res = await app.request('/api')
-      expect(res.status).toBe(200)
-      expect(res.headers.get('X-Custom')).toBe('Hello')
-      expect(await res.json()).toEqual({ foo: 'bar' })
-    })
+  it('Should return 200 response - /api', async () => {
+    const res = await app.request('/api')
+    expect(res.status).toBe(200)
+    expect(res.headers.get('X-Custom')).toBe('Hello')
+    expect(await res.json()).toEqual({ foo: 'bar' })
+  })
 
-    it('Should return 200 response - POST /api', async () => {
-      const res = await app.request('/api', {
-        method: 'POST',
-      })
-      expect(res.status).toBe(201)
-      expect(await res.json()).toEqual({
-        ok: true,
-        message: 'created',
-      })
+  it('Should return 200 response - POST /api', async () => {
+    const res = await app.request('/api', {
+      method: 'POST',
     })
+    expect(res.status).toBe(201)
+    expect(await res.json()).toEqual({
+      ok: true,
+      message: 'created',
+    })
+  })
+})
+
+describe('Nested Layouts', () => {
+  const ROUTES = import.meta.glob('./app-nested/routes/**/[a-z[-][a-z-_[]*.(tsx|ts)', {
+    eager: true,
+  })
+
+  const RENDERER = import.meta.glob('./app-nested/routes/**/_renderer.tsx', {
+    eager: true,
+  })
+
+  const app = createApp({
+    root: './app-nested/routes',
+    ROUTES: ROUTES as any,
+    RENDERER: RENDERER as any,
+  })
+
+  it('Should return 200 response - /nested', async () => {
+    const res = await app.request('/nested')
+    expect(res.status).toBe(200)
+    expect(await res.text()).toBe('<main><h1>Nested</h1></main>')
+  })
+
+  it('Should return 200 response - /nested/foo', async () => {
+    const res = await app.request('/nested/foo')
+    expect(res.status).toBe(200)
+    expect(await res.text()).toBe('<main><nav>foo menu</nav><h1>Foo</h1></main>')
+  })
+
+  it('Should return 200 response - /nested/foo/bar', async () => {
+    const res = await app.request('/nested/foo/bar')
+    expect(res.status).toBe(200)
+    expect(await res.text()).toBe('<main><nav>foo menu</nav><nav>bar menu</nav><h1>Bar</h1></main>')
+  })
+
+  it('Should return 200 response - /nested/foo/bar/baz', async () => {
+    const res = await app.request('/nested/foo/bar/baz')
+    expect(res.status).toBe(200)
+    expect(await res.text()).toBe('<main><nav>foo menu</nav><nav>bar menu</nav><h1>Baz</h1></main>')
   })
 })