diff --git a/_sources/af1/af1.4.ipynb b/_sources/af1/af1.4.ipynb
new file mode 100644
index 0000000..9cbf6c2
--- /dev/null
+++ b/_sources/af1/af1.4.ipynb
@@ -0,0 +1,715 @@
+{
+ "cells": [
+ {
+ "cell_type": "markdown",
+ "metadata": {
+ "id": "LBNBl2exUYWu"
+ },
+ "source": [
+ "# Af1.4\n",
+ "\n",
+ "The **[Af1.4](af1.4): _Anopheles gambiae_ data resource** contains single nucleotide polymorphism (SNP) calls and SNP haplotypes from whole-genome sequencing of 733 mosquitoes.\n",
+ "\n",
+ "More information about this release can be found in the [data resource website](https://www.malariagen.net/data_package/af14-anopheles-funestus-data-resource/). \n",
+ "\n",
+ "This page provides an introduction to open data resources released as part of `Af1.4`. \n",
+ "\n",
+ "If you have any questions about this guide or how to use the data, please [start a new discussion](https://github.com/malariagen/vector-public-data/discussions/new) on the malariagen/vector-open-data repo on GitHub. If you find any bugs, please [raise an issue](https://github.com/malariagen/vector-public-data/issues/new/choose)."
+ ]
+ },
+ {
+ "cell_type": "markdown",
+ "metadata": {
+ "id": "kJqs4cXppk8j"
+ },
+ "source": [
+ "## Terms of use\n",
+ "\n",
+ "Data from this project will be made publicly available before journal publication. Unless otherwise stated, analyses of project data are ongoing and publications are in preparation by project partners, and it is not permitted to use project data for publication (including any type of communication with the general public) without prior permission from the originating partner studies. \n",
+ "\n",
+ "Although malaria is generally an endemic rather than an epidemic disease, and the focus of this project is on surveillance of disease vectors rather than pathogens, our data terms of use build on MalariaGEN's approach to data sharing, and adopt norms which have been established for rapid sharing of pathogen genomic data during disease outbreaks. The primary rationale for this approach is that malaria remains a public health emergency, where ethically appropriate and rapid sharing of genomic surveillance data can help to detect and respond to biological threats such as new forms of insecticide resistance, and to adapt malaria vector control strategies to different settings and changing circumstances.\n",
+ "\n",
+ "The publication embargo for all data on this release will expire on the **16th of August 2026**. \n",
+ "\n",
+ "If you have any questions about the terms of use, please email [support@malariagen.net](mailto:support@malariagen.net)"
+ ]
+ },
+ {
+ "cell_type": "markdown",
+ "metadata": {
+ "id": "iNSicUCtpk8j"
+ },
+ "source": [
+ "## Partner studies\n",
+ "\n",
+ "- [1188-VO-SN-NIANG](https://www.malariagen.net/network/where-we-work/1188-VO-SN-NIANG) - _Anopheles gambiae_ vector surveillance in Senegal.\n",
+ "\n",
+ "- [1330-VO-GN-LAMA](https://www.malariagen.net/network/where-we-work/1330-VO-GN-LAMA) - _Anopheles gambiae_ vector surveillance in Guinea.\n",
+ "\n",
+ "- [1354-VO-KE-DONNELLY](https://www.malariagen.net/network/where-we-work/1354-VO-KE-DONNELLY) - _Anopheles_ vector surveillance in Kenya."
+ ]
+ },
+ {
+ "cell_type": "markdown",
+ "metadata": {
+ "id": "5RHbe7N6pk8k"
+ },
+ "source": [
+ "## Whole-genome sequencing and variant calling\n",
+ "\n",
+ "All samples in `Af1.4` have been sequenced individually to high coverage using Illumina technology at the Wellcome Sanger Institute. These sequence data have then been analysed to identify genetic variants such as single nucleotide polymorphisms (SNPs). After variant calling, both the samples and the variants have been through a range of quality control analyses, to ensure the data are of high quality. Both the raw sequence data and the curated variant calls are openly available for download and analysis. "
+ ]
+ },
+ {
+ "cell_type": "markdown",
+ "metadata": {
+ "id": "9Hfchko2pk8l"
+ },
+ "source": [
+ "## Data hosting\n",
+ "\n",
+ "Data from `Af1.4` are hosted by several different services. \n",
+ "\n",
+ "The SNP data have also been uploaded to Google Cloud, and can be analysed directly within the cloud without having to download or copy any data, including via free interactive computing services such as [MyBinder](https://gke.mybinder.org/) and [Google Colab](https://colab.research.google.com/). Further information about analysing these data in the cloud is provided in the [cloud data access guide](cloud)."
+ ]
+ },
+ {
+ "cell_type": "markdown",
+ "metadata": {
+ "id": "lTJ_EnvOpk8l"
+ },
+ "source": [
+ "## Sample sets\n",
+ "\n",
+ "The samples included in `Af1.4` have been organised into 1 sample set. \n",
+ "\n",
+ "Each sample set corresponds to a set of mosquito specimens from a contributing study. Study details can be found in the partner studies webpages listed above."
+ ]
+ },
+ {
+ "cell_type": "code",
+ "execution_count": 1,
+ "metadata": {
+ "colab": {
+ "base_uri": "https://localhost:8080/"
+ },
+ "id": "hGA4d7Yrpk8m",
+ "outputId": "c29827c1-0361-4926-c227-8f6e76c2a497",
+ "tags": [
+ "remove-input"
+ ]
+ },
+ "outputs": [],
+ "source": [
+ "%pip install -qq malariagen_data"
+ ]
+ },
+ {
+ "cell_type": "markdown",
+ "metadata": {},
+ "source": [
+ "**Note:** To access the `Af1.4` release, you need to use the `pre=True` flag. \n",
+ "\n",
+ "This flag is used when more data will be added to this release, for the case of `Af1.4`, CNV data for the sample sets on this release will be included at a future date."
+ ]
+ },
+ {
+ "cell_type": "code",
+ "execution_count": 1,
+ "metadata": {
+ "id": "AnmzLmEgpk8n",
+ "tags": [
+ "remove-input"
+ ]
+ },
+ "outputs": [
+ {
+ "data": {
+ "application/javascript": [
+ "'use strict';\n",
+ "(function(root) {\n",
+ " function now() {\n",
+ " return new Date();\n",
+ " }\n",
+ "\n",
+ " const force = true;\n",
+ "\n",
+ " if (typeof root._bokeh_onload_callbacks === \"undefined\" || force === true) {\n",
+ " root._bokeh_onload_callbacks = [];\n",
+ " root._bokeh_is_loading = undefined;\n",
+ " }\n",
+ "\n",
+ "const JS_MIME_TYPE = 'application/javascript';\n",
+ " const HTML_MIME_TYPE = 'text/html';\n",
+ " const EXEC_MIME_TYPE = 'application/vnd.bokehjs_exec.v0+json';\n",
+ " const CLASS_NAME = 'output_bokeh rendered_html';\n",
+ "\n",
+ " /**\n",
+ " * Render data to the DOM node\n",
+ " */\n",
+ " function render(props, node) {\n",
+ " const script = document.createElement(\"script\");\n",
+ " node.appendChild(script);\n",
+ " }\n",
+ "\n",
+ " /**\n",
+ " * Handle when an output is cleared or removed\n",
+ " */\n",
+ " function handleClearOutput(event, handle) {\n",
+ " function drop(id) {\n",
+ " const view = Bokeh.index.get_by_id(id)\n",
+ " if (view != null) {\n",
+ " view.model.document.clear()\n",
+ " Bokeh.index.delete(view)\n",
+ " }\n",
+ " }\n",
+ "\n",
+ " const cell = handle.cell;\n",
+ "\n",
+ " const id = cell.output_area._bokeh_element_id;\n",
+ " const server_id = cell.output_area._bokeh_server_id;\n",
+ "\n",
+ " // Clean up Bokeh references\n",
+ " if (id != null) {\n",
+ " drop(id)\n",
+ " }\n",
+ "\n",
+ " if (server_id !== undefined) {\n",
+ " // Clean up Bokeh references\n",
+ " const cmd_clean = \"from bokeh.io.state import curstate; print(curstate().uuid_to_server['\" + server_id + \"'].get_sessions()[0].document.roots[0]._id)\";\n",
+ " cell.notebook.kernel.execute(cmd_clean, {\n",
+ " iopub: {\n",
+ " output: function(msg) {\n",
+ " const id = msg.content.text.trim()\n",
+ " drop(id)\n",
+ " }\n",
+ " }\n",
+ " });\n",
+ " // Destroy server and session\n",
+ " const cmd_destroy = \"import bokeh.io.notebook as ion; ion.destroy_server('\" + server_id + \"')\";\n",
+ " cell.notebook.kernel.execute(cmd_destroy);\n",
+ " }\n",
+ " }\n",
+ "\n",
+ " /**\n",
+ " * Handle when a new output is added\n",
+ " */\n",
+ " function handleAddOutput(event, handle) {\n",
+ " const output_area = handle.output_area;\n",
+ " const output = handle.output;\n",
+ "\n",
+ " // limit handleAddOutput to display_data with EXEC_MIME_TYPE content only\n",
+ " if ((output.output_type != \"display_data\") || (!Object.prototype.hasOwnProperty.call(output.data, EXEC_MIME_TYPE))) {\n",
+ " return\n",
+ " }\n",
+ "\n",
+ " const toinsert = output_area.element.find(\".\" + CLASS_NAME.split(' ')[0]);\n",
+ "\n",
+ " if (output.metadata[EXEC_MIME_TYPE][\"id\"] !== undefined) {\n",
+ " toinsert[toinsert.length - 1].firstChild.textContent = output.data[JS_MIME_TYPE];\n",
+ " // store reference to embed id on output_area\n",
+ " output_area._bokeh_element_id = output.metadata[EXEC_MIME_TYPE][\"id\"];\n",
+ " }\n",
+ " if (output.metadata[EXEC_MIME_TYPE][\"server_id\"] !== undefined) {\n",
+ " const bk_div = document.createElement(\"div\");\n",
+ " bk_div.innerHTML = output.data[HTML_MIME_TYPE];\n",
+ " const script_attrs = bk_div.children[0].attributes;\n",
+ " for (let i = 0; i < script_attrs.length; i++) {\n",
+ " toinsert[toinsert.length - 1].firstChild.setAttribute(script_attrs[i].name, script_attrs[i].value);\n",
+ " toinsert[toinsert.length - 1].firstChild.textContent = bk_div.children[0].textContent\n",
+ " }\n",
+ " // store reference to server id on output_area\n",
+ " output_area._bokeh_server_id = output.metadata[EXEC_MIME_TYPE][\"server_id\"];\n",
+ " }\n",
+ " }\n",
+ "\n",
+ " function register_renderer(events, OutputArea) {\n",
+ "\n",
+ " function append_mime(data, metadata, element) {\n",
+ " // create a DOM node to render to\n",
+ " const toinsert = this.create_output_subarea(\n",
+ " metadata,\n",
+ " CLASS_NAME,\n",
+ " EXEC_MIME_TYPE\n",
+ " );\n",
+ " this.keyboard_manager.register_events(toinsert);\n",
+ " // Render to node\n",
+ " const props = {data: data, metadata: metadata[EXEC_MIME_TYPE]};\n",
+ " render(props, toinsert[toinsert.length - 1]);\n",
+ " element.append(toinsert);\n",
+ " return toinsert\n",
+ " }\n",
+ "\n",
+ " /* Handle when an output is cleared or removed */\n",
+ " events.on('clear_output.CodeCell', handleClearOutput);\n",
+ " events.on('delete.Cell', handleClearOutput);\n",
+ "\n",
+ " /* Handle when a new output is added */\n",
+ " events.on('output_added.OutputArea', handleAddOutput);\n",
+ "\n",
+ " /**\n",
+ " * Register the mime type and append_mime function with output_area\n",
+ " */\n",
+ " OutputArea.prototype.register_mime_type(EXEC_MIME_TYPE, append_mime, {\n",
+ " /* Is output safe? */\n",
+ " safe: true,\n",
+ " /* Index of renderer in `output_area.display_order` */\n",
+ " index: 0\n",
+ " });\n",
+ " }\n",
+ "\n",
+ " // register the mime type if in Jupyter Notebook environment and previously unregistered\n",
+ " if (root.Jupyter !== undefined) {\n",
+ " const events = require('base/js/events');\n",
+ " const OutputArea = require('notebook/js/outputarea').OutputArea;\n",
+ "\n",
+ " if (OutputArea.prototype.mime_types().indexOf(EXEC_MIME_TYPE) == -1) {\n",
+ " register_renderer(events, OutputArea);\n",
+ " }\n",
+ " }\n",
+ " if (typeof (root._bokeh_timeout) === \"undefined\" || force === true) {\n",
+ " root._bokeh_timeout = Date.now() + 5000;\n",
+ " root._bokeh_failed_load = false;\n",
+ " }\n",
+ "\n",
+ " const NB_LOAD_WARNING = {'data': {'text/html':\n",
+ " \"
\\n\"+\n",
+ " \"
\\n\"+\n",
+ " \"BokehJS does not appear to have successfully loaded. If loading BokehJS from CDN, this \\n\"+\n",
+ " \"may be due to a slow or bad network connection. Possible fixes:\\n\"+\n",
+ " \"
\\n\"+\n",
+ " \"
\\n\"+\n",
+ " \"
re-rerun `output_notebook()` to attempt to load from CDN again, or
\\n\"+\n \"BokehJS does not appear to have successfully loaded. If loading BokehJS from CDN, this \\n\"+\n \"may be due to a slow or bad network connection. Possible fixes:\\n\"+\n \"
\\n\"+\n \"
\\n\"+\n \"
re-rerun `output_notebook()` to attempt to load from CDN again, or
The Af1.4: Anopheles gambiae data resource contains single nucleotide polymorphism (SNP) calls and SNP haplotypes from whole-genome sequencing of 733 mosquitoes.
This page provides an introduction to open data resources released as part of Af1.4.
+
If you have any questions about this guide or how to use the data, please start a new discussion on the malariagen/vector-open-data repo on GitHub. If you find any bugs, please raise an issue.
Data from this project will be made publicly available before journal publication. Unless otherwise stated, analyses of project data are ongoing and publications are in preparation by project partners, and it is not permitted to use project data for publication (including any type of communication with the general public) without prior permission from the originating partner studies.
+
Although malaria is generally an endemic rather than an epidemic disease, and the focus of this project is on surveillance of disease vectors rather than pathogens, our data terms of use build on MalariaGEN’s approach to data sharing, and adopt norms which have been established for rapid sharing of pathogen genomic data during disease outbreaks. The primary rationale for this approach is that malaria remains a public health emergency, where ethically appropriate and rapid sharing of genomic surveillance data can help to detect and respond to biological threats such as new forms of insecticide resistance, and to adapt malaria vector control strategies to different settings and changing circumstances.
+
The publication embargo for all data on this release will expire on the 16th of August 2026.
All samples in Af1.4 have been sequenced individually to high coverage using Illumina technology at the Wellcome Sanger Institute. These sequence data have then been analysed to identify genetic variants such as single nucleotide polymorphisms (SNPs). After variant calling, both the samples and the variants have been through a range of quality control analyses, to ensure the data are of high quality. Both the raw sequence data and the curated variant calls are openly available for download and analysis.
Data from Af1.4 are hosted by several different services.
+
The SNP data have also been uploaded to Google Cloud, and can be analysed directly within the cloud without having to download or copy any data, including via free interactive computing services such as MyBinder and Google Colab. Further information about analysing these data in the cloud is provided in the cloud data access guide.
The samples included in Af1.4 have been organised into 1 sample set.
+
Each sample set corresponds to a set of mosquito specimens from a contributing study. Study details can be found in the partner studies webpages listed above.
+
+
+
Note: To access the Af1.4 release, you need to use the pre=True flag.
+
This flag is used when more data will be added to this release, for the case of Af1.4, CNV data for the sample sets on this release will be included at a future date.
+
+
+
+
+
+
+
+
+
+
+
+
+
sample_set
+
sample_count
+
+
+
study_id
+
+
+
+
+
+
+
1188-VO-SN-NIANG
+
1188-VO-NIANG-NIEL-SN-2304-VMF00259
+
71
+
+
+
1330-VO-GN-LAMA
+
1330-VO-GN-LAMA-VMF00250
+
196
+
+
+
1354-VO-KE-DONNELLY
+
1354-VO-KE-DONNELLY-VMF00281
+
466
+
+
+
+
+
+
Here is a more detailed breakdown of the samples contained within this sample set, summarised by country, year of collection, and species:
+
+
+
+
+
+
+
+
+
+
+
+
+
+
taxon
+
funestus
+
+
+
study_id
+
sample_set
+
country
+
year
+
+
+
+
+
+
1188-VO-SN-NIANG
+
1188-VO-NIANG-NIEL-SN-2304-VMF00259
+
Senegal
+
2020
+
11
+
+
+
2021
+
16
+
+
+
2022
+
44
+
+
+
1330-VO-GN-LAMA
+
1330-VO-GN-LAMA-VMF00250
+
Guinea
+
2022
+
196
+
+
+
1354-VO-KE-DONNELLY
+
1354-VO-KE-DONNELLY-VMF00281
+
Kenya
+
2023
+
466
+
+
+
+
+
+
Note that there can be multiple sampling sites represented within the same sample set.
We hope this page has provided a useful introduction to the Af1.4 data resource. If you would like to start working with these data, please visit the cloud data access guide or the data download guide or continue browsing the other documentation on this site.
+
If you have any questions about the data and how to use them, please do get in touch by starting a new discussion on the malariagen/vector-data repository on GitHub.
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
\ No newline at end of file
diff --git a/objects.inv b/objects.inv
index 9b42e1d..ce0930a 100644
Binary files a/objects.inv and b/objects.inv differ
diff --git a/searchindex.js b/searchindex.js
index 630bec7..fe94bcc 100644
--- a/searchindex.js
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