FreeBSD 14.0-RELEASE was installed on a 64 bit virtual machine with 4,096 MB of RAM, 4 processors, 20 GB hard disk. The KDE desktop was installed using the following commands:
pkg install --quiet --yes kde5 plasma5-sddm-kcm sddm xorg
sysrc dbus_enable="YES" && service dbus start
sysrc sddm_enable="YES" && service sddm start
Firefox was then installed with:
pkg install www/firefox
The circularMT_64.exe file and the sequence.gb file were downloaded from the GitHub (https://github.com/msjimc/circularMT) 'Program' and 'Example data' folders to the user's Download folder (~/Downloads) using FireFox.
- The account performing the installation must have admin rights.
If you have admin rights elevate the terminal to raised privileges with:
su -l root
and then enter root's password.
The FreeBSD web site contains instructions on how to install Wine here. It suggest that you first install wine-gecko which contains web browser functions that some programs expect:
pkg install wine-gecko
Next it suggests installing wine-mono which is needed to run .NET applications:
pkg install wine-mono
Once these have been installed wine can be installed using:
pkg install wine
The version of wine can then be determined with:
wine64 --version
This installation installs wine64 which is the 64 bit version that requires 64 bit programs
FreeBSD will not run an application from a terminal with root privileges, so return to your normal account with Ctrl + D before running circularMT_64.exe with:
wine64 ~/Downloads/circularMT_64.exe
This should start circular_64.exe after a configuration step that only occurs when wine is run for the first time (Figure 1).
Figure 1: circularMT_64.exe running on FreeBSD 14.0-RELEASE with the KDE5 desktop.
Once imported, the mitochondrial genome can be modified as described in the Guide Figure 2.
Figure 2