diff --git a/src/OpenTelemetry.Instrumentation.AspNet/Implementation/HttpInListener.cs b/src/OpenTelemetry.Instrumentation.AspNet/Implementation/HttpInListener.cs
index 379a57b2c6..908ca21081 100644
--- a/src/OpenTelemetry.Instrumentation.AspNet/Implementation/HttpInListener.cs
+++ b/src/OpenTelemetry.Instrumentation.AspNet/Implementation/HttpInListener.cs
@@ -95,7 +95,7 @@ private void OnStartActivity(Activity activity, HttpContext context)
             }
 
             activity.SetTag(SemanticConventions.AttributeHttpTarget, path);
-            activity.SetTag(SemanticConventions.AttributeHttpUserAgent, request.UserAgent);
+            activity.SetTag(SemanticConventions.AttributeUserAgentOriginal, request.UserAgent);
             activity.SetTag(SemanticConventions.AttributeHttpUrl, GetUriTagValueFromRequestUri(request.Url));
 
             try
diff --git a/src/Shared/SemanticConventions.cs b/src/Shared/SemanticConventions.cs
index d6e811990d..b960a9409e 100644
--- a/src/Shared/SemanticConventions.cs
+++ b/src/Shared/SemanticConventions.cs
@@ -108,5 +108,6 @@ internal static class SemanticConventions
     public const string AttributeNetworkProtocolVersion = "network.protocol.version"; // replaces: "http.flavor" (AttributeHttpFlavor)
     public const string AttributeServerAddress = "server.address"; // replaces: "net.host.name" (AttributeNetHostName)
     public const string AttributeServerPort = "server.port"; // replaces: "net.host.port" (AttributeNetHostPort)
+    public const string AttributeUserAgentOriginal = "user_agent.original"; // replaces: http.user_agent (AttributeHttpUserAgent)
 #pragma warning restore CS1591 // Missing XML comment for publicly visible type or member
 }
diff --git a/test/OpenTelemetry.Instrumentation.AspNet.Tests/HttpInListenerTests.cs b/test/OpenTelemetry.Instrumentation.AspNet.Tests/HttpInListenerTests.cs
index a1947507c8..45a89e5580 100644
--- a/test/OpenTelemetry.Instrumentation.AspNet.Tests/HttpInListenerTests.cs
+++ b/test/OpenTelemetry.Instrumentation.AspNet.Tests/HttpInListenerTests.cs
@@ -142,7 +142,7 @@ public void AspNetRequestsAreCollectedSuccessfully(
         Assert.Equal(expectedOriginalRequestMethod, span.GetTagValue("http.request.method_original"));
 
         Assert.Equal(HttpContext.Current.Request.Path, span.GetTagValue(SemanticConventions.AttributeHttpTarget) as string);
-        Assert.Equal(HttpContext.Current.Request.UserAgent, span.GetTagValue(SemanticConventions.AttributeHttpUserAgent) as string);
+        Assert.Equal("Custom User Agent v1.2.3", span.GetTagValue("user_agent.original"));
 
         if (recordException)
         {
diff --git a/test/OpenTelemetry.Instrumentation.AspNet.Tests/TestHttpWorkerRequest.cs b/test/OpenTelemetry.Instrumentation.AspNet.Tests/TestHttpWorkerRequest.cs
index c9b1bb5058..80b37bbc81 100644
--- a/test/OpenTelemetry.Instrumentation.AspNet.Tests/TestHttpWorkerRequest.cs
+++ b/test/OpenTelemetry.Instrumentation.AspNet.Tests/TestHttpWorkerRequest.cs
@@ -8,6 +8,16 @@ namespace OpenTelemetry.Instrumentation.AspNet.Tests;
 
 internal class TestHttpWorkerRequest : HttpWorkerRequest
 {
+    public override string GetKnownRequestHeader(int index)
+    {
+        if (index == 39)
+        {
+            return "Custom User Agent v1.2.3";
+        }
+
+        return base.GetKnownRequestHeader(index);
+    }
+
     public override void EndOfRequest()
     {
         throw new NotImplementedException();