diff --git a/tests/e2e/instrumentation-java/00-install-instrumentation.yaml b/tests/e2e/instrumentation-java/00-install-instrumentation.yaml
index 6c1347b139..3e8f556519 100644
--- a/tests/e2e/instrumentation-java/00-install-instrumentation.yaml
+++ b/tests/e2e/instrumentation-java/00-install-instrumentation.yaml
@@ -3,11 +3,26 @@ kind: Instrumentation
 metadata:
   name: java
 spec:
-  exporter:
-    endpoint: http://localhost:4317
+  env:
+    - name: OTEL_TRACES_EXPORTER
+      value: otlp
+    - name: OTEL_EXPORTER_OTLP_ENDPOINT
+      value: http://localhost:4317
+    - name: OTEL_EXPORTER_OTLP_TIMEOUT
+      value: "20"
+    - name: OTEL_TRACES_SAMPLER
+      value: parentbased_traceidratio
+    - name: OTEL_TRACES_SAMPLER_ARG
+      value: "0.85"
   propagators:
     - jaeger
     - b3
   sampler:
     type: parentbased_traceidratio
     argument: "0.25"
+  java:
+    env:
+    - name: OTEL_JAVAAGENT_DEBUG
+      value: "true"
+    - name: OTEL_INSTRUMENTATION_JDBC_ENABLED
+      value: "false"
diff --git a/tests/e2e/instrumentation-java/01-assert.yaml b/tests/e2e/instrumentation-java/01-assert.yaml
index 63b4bd8330..cdc33dcc36 100644
--- a/tests/e2e/instrumentation-java/01-assert.yaml
+++ b/tests/e2e/instrumentation-java/01-assert.yaml
@@ -10,21 +10,29 @@ spec:
   containers:
   - name: myapp
     env:
-    - name: OTEL_SERVICE_NAME
-      value: my-deployment-with-sidecar
+    - name: OTEL_JAVAAGENT_DEBUG
+      value: "true"
+    - name: OTEL_INSTRUMENTATION_JDBC_ENABLED
+      value: "false"
+    - name: JAVA_TOOL_OPTIONS
+      value: " -javaagent:/otel-auto-instrumentation/javaagent.jar"
+    - name: OTEL_TRACES_EXPORTER
+      value: otlp
     - name: OTEL_EXPORTER_OTLP_ENDPOINT
       value: http://localhost:4317
+    - name: OTEL_EXPORTER_OTLP_TIMEOUT
+      value: "20"
+    - name: OTEL_TRACES_SAMPLER
+      value: parentbased_traceidratio
+    - name: OTEL_TRACES_SAMPLER_ARG
+      value: "0.85"
+    - name: OTEL_SERVICE_NAME
+      value: my-deployment-with-sidecar
     - name: OTEL_RESOURCE_ATTRIBUTES_POD_NAME
     - name: OTEL_RESOURCE_ATTRIBUTES_NODE_NAME
     - name: OTEL_RESOURCE_ATTRIBUTES
     - name: OTEL_PROPAGATORS
       value: jaeger,b3
-    - name: OTEL_TRACES_SAMPLER
-      value: parentbased_traceidratio
-    - name: OTEL_TRACES_SAMPLER_ARG
-      value: "0.25"
-    - name: JAVA_TOOL_OPTIONS
-      value: " -javaagent:/otel-auto-instrumentation/javaagent.jar"
     volumeMounts:
     - mountPath: /var/run/secrets/kubernetes.io/serviceaccount
     - mountPath: /otel-auto-instrumentation
diff --git a/tests/e2e/instrumentation-nodejs/00-install-instrumentation.yaml b/tests/e2e/instrumentation-nodejs/00-install-instrumentation.yaml
index 9ea4358b9b..b108c33db2 100644
--- a/tests/e2e/instrumentation-nodejs/00-install-instrumentation.yaml
+++ b/tests/e2e/instrumentation-nodejs/00-install-instrumentation.yaml
@@ -3,8 +3,25 @@ kind: Instrumentation
 metadata:
   name: nodejs
 spec:
-  exporter:
-    endpoint: http://localhost:4317
+  env:
+    - name: OTEL_TRACES_EXPORTER
+      value: otlp
+    - name: OTEL_EXPORTER_OTLP_ENDPOINT
+      value: http://localhost:4317
+    - name: OTEL_EXPORTER_OTLP_TIMEOUT
+      value: "20"
+    - name: OTEL_TRACES_SAMPLER
+      value: parentbased_traceidratio
+    - name: OTEL_TRACES_SAMPLER_ARG
+      value: "0.85"
   propagators:
     - jaeger
     - b3
+  sampler:
+    type: parentbased_traceidratio
+    argument: "0.25"
+  nodejs:
+    env:
+      - name: OTEL_NODEJS_DEBUG
+        value: "true"
+
diff --git a/tests/e2e/instrumentation-nodejs/01-assert.yaml b/tests/e2e/instrumentation-nodejs/01-assert.yaml
index b796a5b8e5..d327e85962 100644
--- a/tests/e2e/instrumentation-nodejs/01-assert.yaml
+++ b/tests/e2e/instrumentation-nodejs/01-assert.yaml
@@ -10,17 +10,27 @@ spec:
   containers:
   - name: myapp
     env:
-    - name: OTEL_SERVICE_NAME
-      value: my-deployment-with-sidecar
+    - name: OTEL_NODEJS_DEBUG
+      value: "true"
+    - name: NODE_OPTIONS
+      value: " --require /otel-auto-instrumentation/autoinstrumentation.js"
+    - name: OTEL_TRACES_EXPORTER
+      value: otlp
     - name: OTEL_EXPORTER_OTLP_ENDPOINT
       value: http://localhost:4317
+    - name: OTEL_EXPORTER_OTLP_TIMEOUT
+      value: "20"
+    - name: OTEL_TRACES_SAMPLER
+      value: parentbased_traceidratio
+    - name: OTEL_TRACES_SAMPLER_ARG
+      value: "0.85"
+    - name: OTEL_SERVICE_NAME
+      value: my-deployment-with-sidecar
     - name: OTEL_RESOURCE_ATTRIBUTES_POD_NAME
     - name: OTEL_RESOURCE_ATTRIBUTES_NODE_NAME
     - name: OTEL_RESOURCE_ATTRIBUTES
     - name: OTEL_PROPAGATORS
       value: jaeger,b3
-    - name: NODE_OPTIONS
-      value: " --require /otel-auto-instrumentation/autoinstrumentation.js"
     volumeMounts:
     - mountPath: /var/run/secrets/kubernetes.io/serviceaccount
     - mountPath: /otel-auto-instrumentation
diff --git a/tests/e2e/instrumentation-python/00-install-instrumentation.yaml b/tests/e2e/instrumentation-python/00-install-instrumentation.yaml
index d41827bf8f..4c6c7a239d 100644
--- a/tests/e2e/instrumentation-python/00-install-instrumentation.yaml
+++ b/tests/e2e/instrumentation-python/00-install-instrumentation.yaml
@@ -3,8 +3,24 @@ kind: Instrumentation
 metadata:
   name: python
 spec:
-  exporter:
-    endpoint: http://localhost:4318/v1/traces
+  env:
+    - name: OTEL_EXPORTER_OTLP_TIMEOUT
+      value: "20"
+    - name: OTEL_TRACES_SAMPLER
+      value: parentbased_traceidratio
+    - name: OTEL_TRACES_SAMPLER_ARG
+      value: "0.85"
   propagators:
     - jaeger
     - b3
+  sampler:
+    type: parentbased_traceidratio
+    argument: "0.25"
+  python:
+    env:
+      - name: OTEL_LOG_LEVEL
+        value: "debug"
+      - name: OTEL_TRACES_EXPORTER
+        value: otlp_proto_http
+      - name: OTEL_EXPORTER_OTLP_ENDPOINT
+        value: http://localhost:4317
\ No newline at end of file
diff --git a/tests/e2e/instrumentation-python/01-assert.yaml b/tests/e2e/instrumentation-python/01-assert.yaml
index 35ea2acb80..105cfd30cd 100644
--- a/tests/e2e/instrumentation-python/01-assert.yaml
+++ b/tests/e2e/instrumentation-python/01-assert.yaml
@@ -10,19 +10,27 @@ spec:
   containers:
   - name: myapp
     env:
+    - name: OTEL_LOG_LEVEL
+      value: "debug"
+    - name: OTEL_TRACES_EXPORTER
+      value: otlp_proto_http
+    - name: OTEL_EXPORTER_OTLP_ENDPOINT
+      value: http://localhost:4317
+    - name: PYTHONPATH
+      value: "/otel-auto-instrumentation/opentelemetry/instrumentation/auto_instrumentation:/otel-auto-instrumentation"
+    - name: OTEL_EXPORTER_OTLP_TIMEOUT
+      value: "20"
+    - name: OTEL_TRACES_SAMPLER
+      value: parentbased_traceidratio
+    - name: OTEL_TRACES_SAMPLER_ARG
+      value: "0.85"
     - name: OTEL_SERVICE_NAME
       value: my-deployment-with-sidecar
-    - name: OTEL_EXPORTER_OTLP_ENDPOINT
-      value: http://localhost:4318/v1/traces
     - name: OTEL_RESOURCE_ATTRIBUTES_POD_NAME
     - name: OTEL_RESOURCE_ATTRIBUTES_NODE_NAME
     - name: OTEL_RESOURCE_ATTRIBUTES
     - name: OTEL_PROPAGATORS
       value: jaeger,b3
-    - name: PYTHONPATH
-      value: "/otel-auto-instrumentation/opentelemetry/instrumentation/auto_instrumentation:/otel-auto-instrumentation"
-    - name: OTEL_TRACES_EXPORTER
-      value: otlp_proto_http
     volumeMounts:
     - mountPath: /var/run/secrets/kubernetes.io/serviceaccount
     - mountPath: /otel-auto-instrumentation