diff --git a/.chloggen/fix_validation-stabilizationWindowSeconds.yaml b/.chloggen/fix_validation-stabilizationWindowSeconds.yaml
new file mode 100755
index 0000000000..b90f0ecbd9
--- /dev/null
+++ b/.chloggen/fix_validation-stabilizationWindowSeconds.yaml
@@ -0,0 +1,18 @@
+# One of 'breaking', 'deprecation', 'new_component', 'enhancement', 'bug_fix'
+change_type: bug_fix
+
+# The name of the component, or a single word describing the area of concern, (e.g. collector, target allocator, auto-instrumentation, opamp, github action)
+component: collector-webhook
+
+# A brief description of the change. Surround your text with quotes ("") if it needs to start with a backtick (`).
+note: "Fixed validation of `stabilizationWindowSeconds` in autoscaler behaviour"
+
+# One or more tracking issues related to the change
+issues: [3345]
+
+# (Optional) One or more lines of additional information to render under the primary note.
+# These lines will be padded with 2 spaces and then inserted directly into the document.
+# Use pipe (|) for multiline entries.
+subtext: |
+  The validation of `stabilizationWindowSeconds` in the `autoscaler.behaviour.scale[Up|Down]` incorrectly rejected 0 as an invalid value.
+  This has been fixed to ensure that the value is validated correctly (should be >=0 and <=3600) and the error messsage has been updated to reflect this.
diff --git a/apis/v1beta1/collector_webhook.go b/apis/v1beta1/collector_webhook.go
index e79754b4bd..5a6b80a3b9 100644
--- a/apis/v1beta1/collector_webhook.go
+++ b/apis/v1beta1/collector_webhook.go
@@ -385,13 +385,13 @@ func ValidatePorts(ports []PortsSpec) error {
 func checkAutoscalerSpec(autoscaler *AutoscalerSpec) error {
 	if autoscaler.Behavior != nil {
 		if autoscaler.Behavior.ScaleDown != nil && autoscaler.Behavior.ScaleDown.StabilizationWindowSeconds != nil &&
-			*autoscaler.Behavior.ScaleDown.StabilizationWindowSeconds < int32(1) {
-			return fmt.Errorf("the OpenTelemetry Spec autoscale configuration is incorrect, scaleDown should be one or more")
+			(*autoscaler.Behavior.ScaleDown.StabilizationWindowSeconds < int32(0) || *autoscaler.Behavior.ScaleDown.StabilizationWindowSeconds > 3600) {
+			return fmt.Errorf("the OpenTelemetry Spec autoscale configuration is incorrect, scaleDown.stabilizationWindowSeconds should be >=0 and <=3600")
 		}
 
 		if autoscaler.Behavior.ScaleUp != nil && autoscaler.Behavior.ScaleUp.StabilizationWindowSeconds != nil &&
-			*autoscaler.Behavior.ScaleUp.StabilizationWindowSeconds < int32(1) {
-			return fmt.Errorf("the OpenTelemetry Spec autoscale configuration is incorrect, scaleUp should be one or more")
+			(*autoscaler.Behavior.ScaleUp.StabilizationWindowSeconds < int32(0) || *autoscaler.Behavior.ScaleUp.StabilizationWindowSeconds > 3600) {
+			return fmt.Errorf("the OpenTelemetry Spec autoscale configuration is incorrect, scaleUp.stabilizationWindowSeconds should be >=0 and <=3600")
 		}
 	}
 	if autoscaler.TargetCPUUtilization != nil && *autoscaler.TargetCPUUtilization < int32(1) {
diff --git a/apis/v1beta1/collector_webhook_test.go b/apis/v1beta1/collector_webhook_test.go
index 0b6b915486..d6145543f3 100644
--- a/apis/v1beta1/collector_webhook_test.go
+++ b/apis/v1beta1/collector_webhook_test.go
@@ -17,6 +17,7 @@ package v1beta1_test
 import (
 	"context"
 	"fmt"
+	"math"
 	"os"
 	"testing"
 
@@ -582,6 +583,7 @@ func TestOTELColValidatingWebhook(t *testing.T) {
 	one := int32(1)
 	three := int32(3)
 	five := int32(5)
+	maxInt := int32(math.MaxInt32)
 
 	cfg := v1beta1.Config{}
 	err := yaml.Unmarshal([]byte(cfgYaml), &cfg)
@@ -913,36 +915,68 @@ func TestOTELColValidatingWebhook(t *testing.T) {
 			expectedErr: "minReplicas should be one or more",
 		},
 		{
-			name: "invalid autoscaler scale down",
+			name: "invalid autoscaler scale down stablization window - <0",
 			otelcol: v1beta1.OpenTelemetryCollector{
 				Spec: v1beta1.OpenTelemetryCollectorSpec{
 					Autoscaler: &v1beta1.AutoscalerSpec{
 						MaxReplicas: &three,
 						Behavior: &autoscalingv2.HorizontalPodAutoscalerBehavior{
 							ScaleDown: &autoscalingv2.HPAScalingRules{
-								StabilizationWindowSeconds: &zero,
+								StabilizationWindowSeconds: &minusOne,
 							},
 						},
 					},
 				},
 			},
-			expectedErr: "scaleDown should be one or more",
+			expectedErr: "scaleDown.stabilizationWindowSeconds should be >=0 and <=3600",
 		},
 		{
-			name: "invalid autoscaler scale up",
+			name: "invalid autoscaler scale down stablization window - >3600",
+			otelcol: v1beta1.OpenTelemetryCollector{
+				Spec: v1beta1.OpenTelemetryCollectorSpec{
+					Autoscaler: &v1beta1.AutoscalerSpec{
+						MaxReplicas: &three,
+						Behavior: &autoscalingv2.HorizontalPodAutoscalerBehavior{
+							ScaleDown: &autoscalingv2.HPAScalingRules{
+								StabilizationWindowSeconds: &maxInt,
+							},
+						},
+					},
+				},
+			},
+			expectedErr: "scaleDown.stabilizationWindowSeconds should be >=0 and <=3600",
+		},
+		{
+			name: "invalid autoscaler scale up stablization window - <0",
+			otelcol: v1beta1.OpenTelemetryCollector{
+				Spec: v1beta1.OpenTelemetryCollectorSpec{
+					Autoscaler: &v1beta1.AutoscalerSpec{
+						MaxReplicas: &three,
+						Behavior: &autoscalingv2.HorizontalPodAutoscalerBehavior{
+							ScaleUp: &autoscalingv2.HPAScalingRules{
+								StabilizationWindowSeconds: &minusOne,
+							},
+						},
+					},
+				},
+			},
+			expectedErr: "scaleUp.stabilizationWindowSeconds should be >=0 and <=3600",
+		},
+		{
+			name: "invalid autoscaler scale up stablization window - >3600",
 			otelcol: v1beta1.OpenTelemetryCollector{
 				Spec: v1beta1.OpenTelemetryCollectorSpec{
 					Autoscaler: &v1beta1.AutoscalerSpec{
 						MaxReplicas: &three,
 						Behavior: &autoscalingv2.HorizontalPodAutoscalerBehavior{
 							ScaleUp: &autoscalingv2.HPAScalingRules{
-								StabilizationWindowSeconds: &zero,
+								StabilizationWindowSeconds: &maxInt,
 							},
 						},
 					},
 				},
 			},
-			expectedErr: "scaleUp should be one or more",
+			expectedErr: "scaleUp.stabilizationWindowSeconds should be >=0 and <=3600",
 		},
 		{
 			name: "invalid autoscaler target cpu utilization",