diff --git a/instrumentation/opentelemetry-instrumentation-grpc/pyproject.toml b/instrumentation/opentelemetry-instrumentation-grpc/pyproject.toml
index 8373feaa40..056592dd42 100644
--- a/instrumentation/opentelemetry-instrumentation-grpc/pyproject.toml
+++ b/instrumentation/opentelemetry-instrumentation-grpc/pyproject.toml
@@ -35,12 +35,6 @@ dependencies = [
 instruments = [
   "grpcio ~= 1.27",
 ]
-test = [
-  "opentelemetry-instrumentation-grpc[instruments]",
-  "opentelemetry-sdk ~= 1.12",
-  "opentelemetry-test-utils == 0.45b0.dev",
-  "protobuf ~= 3.13",
-]
 
 [project.entry-points.opentelemetry_instrumentor]
 grpc_client = "opentelemetry.instrumentation.grpc:GrpcInstrumentorClient"
diff --git a/instrumentation/opentelemetry-instrumentation-grpc/test-requirements.txt b/instrumentation/opentelemetry-instrumentation-grpc/test-requirements.txt
new file mode 100644
index 0000000000..56d47af0df
--- /dev/null
+++ b/instrumentation/opentelemetry-instrumentation-grpc/test-requirements.txt
@@ -0,0 +1,20 @@
+asgiref==3.7.2
+attrs==23.2.0
+Deprecated==1.2.14
+grpcio==1.62.0
+importlib-metadata==6.11.0
+iniconfig==2.0.0
+packaging==23.2
+pluggy==1.4.0
+protobuf==3.20.3
+py==1.11.0
+py-cpuinfo==9.0.0
+pytest==7.1.3
+pytest-asyncio==0.23.5
+pytest-benchmark==4.0.0
+tomli==2.0.1
+typing_extensions==4.9.0
+wrapt==1.16.0
+zipp==3.17.0
+-e opentelemetry-instrumentation
+-e instrumentation/opentelemetry-instrumentation-grpc
diff --git a/tox.ini b/tox.ini
index 14b81e3bf0..d55243e8cb 100644
--- a/tox.ini
+++ b/tox.ini
@@ -341,7 +341,7 @@ commands_pre =
 
   confluent-kafka: pip install {toxinidir}/instrumentation/opentelemetry-instrumentation-confluent-kafka[test]
 
-  grpc: pip install {toxinidir}/instrumentation/opentelemetry-instrumentation-grpc[test]
+  grpc: pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-grpc/test-requirements.txt
 
   wsgi: pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-wsgi/test-requirements.txt
 
@@ -571,8 +571,8 @@ commands_pre =
   python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-boto3sqs[test]
   pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-django/test-requirements-3.txt
   pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.txt
-  python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-grpc[test]
   pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-falcon/test-requirements-2.txt
+  pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-grpc/test-requirements.txt
   python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-boto[test]
   pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-flask/test-requirements-2.txt
   pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-sqlalchemy/test-requirements-1.txt