diff --git a/opentelemetry-instrumentation/pyproject.toml b/opentelemetry-instrumentation/pyproject.toml
index db389544c9..a20b005911 100644
--- a/opentelemetry-instrumentation/pyproject.toml
+++ b/opentelemetry-instrumentation/pyproject.toml
@@ -29,9 +29,6 @@ dependencies = [
   "wrapt >= 1.0.0, < 2.0.0",
 ]
 
-[project.optional-dependencies]
-test = []
-
 [project.scripts]
 opentelemetry-bootstrap = "opentelemetry.instrumentation.bootstrap:run"
 opentelemetry-instrument = "opentelemetry.instrumentation.auto_instrumentation:run"
diff --git a/opentelemetry-instrumentation/test-requirements.txt b/opentelemetry-instrumentation/test-requirements.txt
new file mode 100644
index 0000000000..473a423bda
--- /dev/null
+++ b/opentelemetry-instrumentation/test-requirements.txt
@@ -0,0 +1,16 @@
+asgiref==3.7.2
+attrs==23.2.0
+Deprecated==1.2.14
+importlib-metadata==6.11.0
+iniconfig==2.0.0
+packaging==23.2
+pluggy==1.4.0
+py==1.11.0
+py-cpuinfo==9.0.0
+pytest==7.1.3
+pytest-benchmark==4.0.0
+tomli==2.0.1
+typing_extensions==4.10.0
+wrapt==1.16.0
+zipp==3.17.0
+-e opentelemetry-instrumentation
diff --git a/tox.ini b/tox.ini
index 4ceced1210..07e94f58d1 100644
--- a/tox.ini
+++ b/tox.ini
@@ -323,6 +323,8 @@ commands_pre =
   test: pip install opentelemetry-test-utils[test]@{env:CORE_REPO}\#egg=opentelemetry-test-utils&subdirectory=tests/opentelemetry-test-utils
   test: pip install {toxinidir}/opentelemetry-instrumentation
 
+  opentelemetry-instrumentation: pip install -r {toxinidir}/opentelemetry-instrumentation/test-requirements.txt
+
   distro: pip install {toxinidir}/opentelemetry-distro
 
   asgi: pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-asgi/test-requirements.txt
@@ -565,11 +567,11 @@ commands_pre =
   python -m pip install {env:CORE_REPO}\#egg=opentelemetry-sdk&subdirectory=opentelemetry-sdk
   python -m pip install {env:CORE_REPO}\#egg=opentelemetry-test-utils&subdirectory=tests/opentelemetry-test-utils
   python -m pip install -e {toxinidir}/util/opentelemetry-util-http[test]
-  python -m pip install -e {toxinidir}/opentelemetry-instrumentation[test]
   pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-wsgi/test-requirements.txt
   pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-asgi/test-requirements.txt
   pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-dbapi/test-requirements.txt
   pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-botocore/test-requirements.txt
+  pip install -r {toxinidir}/opentelemetry-instrumentation/test-requirements.txt
   python -m pip install -e {toxinidir}/instrumentation/opentelemetry-instrumentation-boto3sqs[test]
   pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-django/test-requirements-3.txt
   pip install -r {toxinidir}/instrumentation/opentelemetry-instrumentation-starlette/test-requirements.txt