diff --git a/CHANGELOG.md b/CHANGELOG.md
index 4c60e3a3a2..bb31a5fd9d 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -45,6 +45,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
   ([#562](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/562))
 - `opentelemetry-instrumentation-django` Fix AttributeError: ResolverMatch object has no attribute route
   ([#581](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/581))
+- `opentelemetry-instrumentation-botocore` Suppress botocore downstream instrumentation like urllib3
+  ([#563](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/563))
 
 ### Added
 - `opentelemetry-instrumentation-httpx` Add `httpx` instrumentation
diff --git a/instrumentation/opentelemetry-instrumentation-botocore/src/opentelemetry/instrumentation/botocore/__init__.py b/instrumentation/opentelemetry-instrumentation-botocore/src/opentelemetry/instrumentation/botocore/__init__.py
index 0439490c5c..e4ac1bad21 100644
--- a/instrumentation/opentelemetry-instrumentation-botocore/src/opentelemetry/instrumentation/botocore/__init__.py
+++ b/instrumentation/opentelemetry-instrumentation-botocore/src/opentelemetry/instrumentation/botocore/__init__.py
@@ -68,6 +68,13 @@
 
 logger = logging.getLogger(__name__)
 
+# A key to a context variable to avoid creating duplicate spans when instrumenting
+# both botocore.client and urllib3.connectionpool.HTTPConnectionPool.urlopen since
+# botocore calls urlopen
+_SUPPRESS_HTTP_INSTRUMENTATION_KEY = context_api.create_key(
+    "suppress_http_instrumentation"
+)
+
 
 # pylint: disable=unused-argument
 def _patched_endpoint_prepare_request(wrapped, instance, args, kwargs):
@@ -161,10 +168,16 @@ def _patched_api_call(self, original_func, instance, args, kwargs):
                         "aws.table_name", api_params["TableName"]
                     )
 
+            token = context_api.attach(
+                context_api.set_value(_SUPPRESS_HTTP_INSTRUMENTATION_KEY, True)
+            )
+
             try:
                 result = original_func(*args, **kwargs)
             except ClientError as ex:
                 error = ex
+            finally:
+                context_api.detach(token)
 
             if error:
                 result = error.response