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Question regarding multiple barcodes #12

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Fhennig94 opened this issue Sep 28, 2017 · 0 comments
Open

Question regarding multiple barcodes #12

Fhennig94 opened this issue Sep 28, 2017 · 0 comments

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@Fhennig94
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Fhennig94 commented Sep 28, 2017 •
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Hello,
I have a question regarding the parameter --barcode_start and barcode_end. If I have 3 barcodes within a 100bp read that span almost to the end, do I just put the barcode end as the end of the read? The sequence between the 3 barcodes should be the same but most likely will have indels/mismatches. Or do I put the barcode_start and barcode_end positions separately for each barcode? Thank you!
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