diff --git a/cdc/sink/mysql_params.go b/cdc/sink/mysql_params.go
index 9f0fec92a41..320ba69bc30 100644
--- a/cdc/sink/mysql_params.go
+++ b/cdc/sink/mysql_params.go
@@ -268,8 +268,6 @@ func generateDSNByParams(
 	dsnCfg.Params["readTimeout"] = params.readTimeout
 	dsnCfg.Params["writeTimeout"] = params.writeTimeout
 	dsnCfg.Params["timeout"] = params.dialTimeout
-	// Since we don't need select, just set default isolation level to read-committed
-	dsnCfg.Params["transaction_isolation"] = fmt.Sprintf(`"%s"`, defaultTxnIsolationRC)
 
 	autoRandom, err := checkTiDBVariable(ctx, testDB, "allow_auto_random_explicit_insert", "1")
 	if err != nil {
@@ -287,6 +285,18 @@ func generateDSNByParams(
 		dsnCfg.Params["tidb_txn_mode"] = txnMode
 	}
 
+	// Since we don't need select, just set default isolation level to read-committed
+	// transaction_isolation is mysql newly introduced variable and will vary from MySQL5.7/MySQL8.0/Mariadb
+	isolation, err := checkTiDBVariable(ctx, testDB, "transaction_isolation", defaultTxnIsolationRC)
+	if err != nil {
+		return "", err
+	}
+	if isolation != "" {
+		dsnCfg.Params["transaction_isolation"] = fmt.Sprintf(`"%s"`, defaultTxnIsolationRC)
+	} else {
+		dsnCfg.Params["tx_isolation"] = fmt.Sprintf(`"%s"`, defaultTxnIsolationRC)
+	}
+
 	dsnClone := dsnCfg.Clone()
 	dsnClone.Passwd = "******"
 	log.Info("sink uri is configured", zap.String("dsn", dsnClone.FormatDSN()))
diff --git a/cdc/sink/mysql_params_test.go b/cdc/sink/mysql_params_test.go
index 075e5d41d26..80a104ab244 100644
--- a/cdc/sink/mysql_params_test.go
+++ b/cdc/sink/mysql_params_test.go
@@ -118,9 +118,66 @@ func TestGenerateDSNByParams(t *testing.T) {
 		}
 	}
 
+	testIsolationParams := func() {
+		db, mock, err := sqlmock.New()
+		require.Nil(t, err)
+		defer db.Close() // nolint:errcheck
+		columns := []string{"Variable_name", "Value"}
+		mock.ExpectQuery("show session variables like 'allow_auto_random_explicit_insert';").WillReturnRows(
+			sqlmock.NewRows(columns).AddRow("allow_auto_random_explicit_insert", "0"),
+		)
+		mock.ExpectQuery("show session variables like 'tidb_txn_mode';").WillReturnRows(
+			sqlmock.NewRows(columns).AddRow("tidb_txn_mode", "pessimistic"),
+		)
+		// simulate error
+		dsn, err := dmysql.ParseDSN("root:123456@tcp(127.0.0.1:4000)/")
+		require.Nil(t, err)
+		params := defaultParams.Clone()
+		var dsnStr string
+		_, err = generateDSNByParams(context.TODO(), dsn, params, db)
+		require.Error(t, err)
+
+		// simulate no transaction_isolation
+		mock.ExpectQuery("show session variables like 'allow_auto_random_explicit_insert';").WillReturnRows(
+			sqlmock.NewRows(columns).AddRow("allow_auto_random_explicit_insert", "0"),
+		)
+		mock.ExpectQuery("show session variables like 'tidb_txn_mode';").WillReturnRows(
+			sqlmock.NewRows(columns).AddRow("tidb_txn_mode", "pessimistic"),
+		)
+		mock.ExpectQuery("show session variables like 'transaction_isolation';").WillReturnError(sql.ErrNoRows)
+		dsnStr, err = generateDSNByParams(context.TODO(), dsn, params, db)
+		require.Nil(t, err)
+		expectedParams := []string{
+			"tx_isolation=%22READ-COMMITTED%22",
+		}
+		for _, param := range expectedParams {
+			require.True(t, strings.Contains(dsnStr, param))
+		}
+
+		// simulate transaction_isolation
+		mock.ExpectQuery("show session variables like 'allow_auto_random_explicit_insert';").WillReturnRows(
+			sqlmock.NewRows(columns).AddRow("allow_auto_random_explicit_insert", "0"),
+		)
+		mock.ExpectQuery("show session variables like 'tidb_txn_mode';").WillReturnRows(
+			sqlmock.NewRows(columns).AddRow("tidb_txn_mode", "pessimistic"),
+		)
+		mock.ExpectQuery("show session variables like 'transaction_isolation';").WillReturnRows(
+			sqlmock.NewRows(columns).AddRow("transaction_isolation", "REPEATED-READ"),
+		)
+		dsnStr, err = generateDSNByParams(context.TODO(), dsn, params, db)
+		require.Nil(t, err)
+		expectedParams = []string{
+			"transaction_isolation=%22READ-COMMITTED%22",
+		}
+		for _, param := range expectedParams {
+			require.True(t, strings.Contains(dsnStr, param))
+		}
+	}
+
 	testDefaultParams()
 	testTimezoneParam()
 	testTimeoutParams()
+	testIsolationParams()
 }
 
 func TestParseSinkURIToParams(t *testing.T) {
diff --git a/cdc/sink/mysql_test.go b/cdc/sink/mysql_test.go
index cc0b119350c..aa69c25da23 100644
--- a/cdc/sink/mysql_test.go
+++ b/cdc/sink/mysql_test.go
@@ -472,6 +472,9 @@ func mockTestDB(adjustSQLMode bool) (*sql.DB, error) {
 	mock.ExpectQuery("show session variables like 'tidb_txn_mode';").WillReturnRows(
 		sqlmock.NewRows(columns).AddRow("tidb_txn_mode", "pessimistic"),
 	)
+	mock.ExpectQuery("show session variables like 'transaction_isolation';").WillReturnRows(
+		sqlmock.NewRows(columns).AddRow("transaction_isolation", "REPEATED-READ"),
+	)
 	mock.ExpectClose()
 	return db, nil
 }
diff --git a/pkg/applier/redo_test.go b/pkg/applier/redo_test.go
index 5d2466bd27e..8c33f61de86 100644
--- a/pkg/applier/redo_test.go
+++ b/pkg/applier/redo_test.go
@@ -137,6 +137,9 @@ func TestApplyDMLs(t *testing.T) {
 			mock.ExpectQuery("show session variables like 'tidb_txn_mode';").WillReturnRows(
 				sqlmock.NewRows(columns).AddRow("tidb_txn_mode", "pessimistic"),
 			)
+			mock.ExpectQuery("show session variables like 'transaction_isolation';").WillReturnRows(
+				sqlmock.NewRows(columns).AddRow("transaction_isolation", "REPEATED-READ"),
+			)
 			mock.ExpectClose()
 			return db, nil
 		}