diff --git a/mypy/subtypes.py b/mypy/subtypes.py
index 3b775a06bd6e..787d5cb89b0a 100644
--- a/mypy/subtypes.py
+++ b/mypy/subtypes.py
@@ -1941,6 +1941,8 @@ def restrict_subtype_away(t: Type, s: Type) -> Type:
                 if (isinstance(get_proper_type(item), AnyType) or not covers_at_runtime(item, s))
             ]
         return UnionType.make_union(new_items)
+    elif isinstance(p_t, TypeVarType):
+        return p_t.copy_modified(upper_bound=restrict_subtype_away(p_t.upper_bound, s))
     elif covers_at_runtime(t, s):
         return UninhabitedType()
     else:
diff --git a/test-data/unit/check-isinstance.test b/test-data/unit/check-isinstance.test
index b7ee38b69d00..8fa1bc1ca1ac 100644
--- a/test-data/unit/check-isinstance.test
+++ b/test-data/unit/check-isinstance.test
@@ -1833,6 +1833,30 @@ def f(x: T) -> None:
     reveal_type(x) # N: Revealed type is "T`-1"
 [builtins fixtures/isinstance.pyi]
 
+[case testIsinstanceAndNegativeNarrowTypeVariableWithUnionBound]
+from typing import Union, TypeVar
+
+class A:
+    a: int
+class B:
+    b: int
+
+T = TypeVar("T", bound=Union[A, B])
+
+def f(x: T) -> T:
+    if isinstance(x, A):
+        reveal_type(x)      # N: Revealed type is "__main__.A"
+        x.a
+        x.b                 # E: "A" has no attribute "b"
+    else:
+        reveal_type(x)      # N: Revealed type is "T`-1"
+        x.a                 # E: "T" has no attribute "a"
+        x.b
+    x.a                     # E: Item "B" of the upper bound "Union[A, B]" of type variable "T" has no attribute "a"
+    x.b                     # E: Item "A" of the upper bound "Union[A, B]" of type variable "T" has no attribute "b"
+    return x
+[builtins fixtures/isinstance.pyi]
+
 [case testIsinstanceAndTypeType]
 from typing import Type
 def f(x: Type[int]) -> None: