diff --git a/mypy/subtypes.py b/mypy/subtypes.py
index c76b3569fdd4..e08620c0c985 100644
--- a/mypy/subtypes.py
+++ b/mypy/subtypes.py
@@ -1603,6 +1603,17 @@ def are_parameters_compatible(
         return True
     trivial_suffix = is_trivial_suffix(right) and not is_proper_subtype
 
+    # def _(*a: Unpack[tuple[object, ...]]) allows any number of arguments, not just infinite.
+    if right_star and isinstance(right_star.typ, UnpackType):
+        right_star_inner_type = get_proper_type(right_star.typ.type)
+        trivial_varargs = (
+            isinstance(right_star_inner_type, Instance)
+            and right_star_inner_type.type.fullname == "builtins.tuple"
+            and len(right_star_inner_type.args) == 1
+        )
+    else:
+        trivial_varargs = False
+
     if (
         right.arg_kinds == [ARG_STAR]
         and isinstance(get_proper_type(right.arg_types[0]), AnyType)
@@ -1640,14 +1651,20 @@ def are_parameters_compatible(
     #           Furthermore, if we're checking for compatibility in all cases,
     #           we confirm that if R accepts an infinite number of arguments,
     #           L must accept the same.
-    def _incompatible(left_arg: FormalArgument | None, right_arg: FormalArgument | None) -> bool:
+    def _incompatible(
+        left_arg: FormalArgument | None, right_arg: FormalArgument | None, varargs: bool
+    ) -> bool:
         if right_arg is None:
             return False
         if left_arg is None:
-            return not allow_partial_overlap and not trivial_suffix
+            return (
+                not allow_partial_overlap
+                and not trivial_suffix
+                and (not varargs or not trivial_varargs)
+            )
         return not is_compat(right_arg.typ, left_arg.typ)
 
-    if _incompatible(left_star, right_star) or _incompatible(left_star2, right_star2):
+    if _incompatible(left_star, right_star, True) or _incompatible(left_star2, right_star2, False):
         return False
 
     # Phase 1b: Check non-star args: for every arg right can accept, left must
@@ -1672,8 +1689,7 @@ def _incompatible(left_arg: FormalArgument | None, right_arg: FormalArgument | N
     # Phase 1c: Check var args. Right has an infinite series of optional positional
     #           arguments. Get all further positional args of left, and make sure
     #           they're more general than the corresponding member in right.
-    # TODO: are we handling UnpackType correctly here?
-    if right_star is not None and not trivial_suffix:
+    if right_star is not None and not trivial_suffix and not trivial_varargs:
         # Synthesize an anonymous formal argument for the right
         right_by_position = right.try_synthesizing_arg_from_vararg(None)
         assert right_by_position is not None
diff --git a/test-data/unit/check-typevar-tuple.test b/test-data/unit/check-typevar-tuple.test
index f49e1b3c6613..2c0d3d9e0d0c 100644
--- a/test-data/unit/check-typevar-tuple.test
+++ b/test-data/unit/check-typevar-tuple.test
@@ -2487,3 +2487,26 @@ class C(Generic[P, R]):
 c: C[int, str]  # E: Can only replace ParamSpec with a parameter types list or another ParamSpec, got "int"
 reveal_type(c.fn)  # N: Revealed type is "def (*Any, **Any)"
 [builtins fixtures/tuple.pyi]
+
+[case testSubtypingWithUnpackAndTuples]
+# This test is convoluted as there is special casing to get past.
+# (for instance, passing a keyword argument is necessary)
+import operator
+from collections.abc import Callable
+from typing_extensions import TypeVarTuple, Unpack, ParamSpec
+
+Ts = TypeVarTuple("Ts")
+P = ParamSpec("P")
+
+def run(func: Callable[[Unpack[Ts]], None], *args: Unpack[Ts], some_kwarg: None) -> None:
+    return func(*args)
+
+def call(func: Callable[P, None], *args: P.args, **kwargs: P.kwargs) -> None:
+    return func(*args, **kwargs)
+
+def foo() -> None:
+    return None
+
+run(foo, some_kwarg=None)
+call(run, foo, some_kwarg=None)
+[builtins fixtures/tuple.pyi]