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change freebayes min depth param #42

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sanjaynagi opened this issue May 3, 2022 · 2 comments
Open

change freebayes min depth param #42

sanjaynagi opened this issue May 3, 2022 · 2 comments

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@sanjaynagi
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sanjaynagi commented May 3, 2022

This needs changing to 4 ideally as previous studies shown variant calling accurate above this level.
already we have missingness filters high so this probably wont make much difference.

@sanjaynagi
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Related -

Should we have a filter for:

  • Sites of poor mapping quality?
  • Sites at homopolymers
  • Sites at exon-intron junctions

and how many SNPs will this remove?

@sanjaynagi
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We should also potentially use a maximum depth filter within freebayes, we have --use-best-n-alleles which may do the same job, however.

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