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palmscan False Negative of Orthomyxoviridae #196

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batson opened this issue Sep 14, 2022 · 2 comments
Open

palmscan False Negative of Orthomyxoviridae #196

batson opened this issue Sep 14, 2022 · 2 comments
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@batson
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batson commented Sep 14, 2022

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Hash: 7603a13b7fcfe7768ded959684192115c6f8947c
Submission time (ISO): 2022-09-14T05:43:31.734Z

Submission was genome of Wuhan Mosquito Virus 6 from NCBI: https://www.ncbi.nlm.nih.gov/nuccore/MW434423.1?report=fasta

@batson batson added the bug Something isn't working label Sep 14, 2022
@ababaian
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Yes this is a known bug :) I'd have sent you this link for palmID for WMV6 otherwise. palmscan PSSM are not hitting Orthomyxoviridae very efficiently, it's a false-negative. You'll need to perform the SRA-list parsing manually against the micro-assembly rdrp1.mu.fa contigs. Once you have that you can walk through the remaining palmID R script to generate the report locally.

Sorry about that. I'll leave this open as a ticket to swing back to it.

@ababaian ababaian changed the title "No viral RdRP identified in input sequence (or a server error occured)" palmscan False Negative of Orthomyxoviridae Sep 14, 2022
@batson
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batson commented Sep 14, 2022

Thanks, @ababaian. I ended up going through all the WMV6 microassembly contigs more or less manually, and finding sequence from Tunesia, the Philippines, and CT (USA) which hadn't made it to GenBank. We're updating the paper accordingly (going from five continents to six!) and citing Serratus!

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