diff --git a/.changeset/clever-bees-float.md b/.changeset/clever-bees-float.md
new file mode 100644
index 000000000000..3a7fbf6ff5dd
--- /dev/null
+++ b/.changeset/clever-bees-float.md
@@ -0,0 +1,5 @@
+---
+'@sveltejs/kit': patch
+---
+
+fix: decode asset URLs in dev when reading them
diff --git a/packages/kit/src/runtime/app/server/index.js b/packages/kit/src/runtime/app/server/index.js
index 3702c171bb6a..2a1ae1765b26 100644
--- a/packages/kit/src/runtime/app/server/index.js
+++ b/packages/kit/src/runtime/app/server/index.js
@@ -52,7 +52,8 @@ export function read(asset) {
 		});
 	}
 
-	const file = DEV && asset.startsWith('/@fs') ? asset : asset.slice(base.length + 1);
+	const file =
+		DEV && asset.startsWith('/@fs') ? decodeURIComponent(asset) : asset.slice(base.length + 1);
 
 	if (file in manifest._.server_assets) {
 		const length = manifest._.server_assets[file];
diff --git a/packages/kit/test/apps/basics/src/routes/read-file/+page.server.js b/packages/kit/test/apps/basics/src/routes/read-file/+page.server.js
index 1b89fef87df1..018f57c4f261 100644
--- a/packages/kit/test/apps/basics/src/routes/read-file/+page.server.js
+++ b/packages/kit/test/apps/basics/src/routes/read-file/+page.server.js
@@ -3,8 +3,9 @@ import { read } from '$app/server';
 import auto from './auto.txt';
 import url from './url.txt?url';
 
-const glob = import.meta.glob('../../../../read-file-test.txt', {
-	as: 'url',
+/** @type {Record<string, { default: string }>} */
+const glob = import.meta.glob('../../../../read-file-test/**', {
+	query: '?url',
 	eager: true
 });
 
@@ -16,6 +17,6 @@ export async function load() {
 	return {
 		auto: await read(auto).text(),
 		url: await read(url).text(),
-		glob: await read(Object.values(glob)[0]).text()
+		glob: await read(Object.values(glob)[0].default).text()
 	};
 }
diff --git a/packages/kit/test/apps/read-file-test.txt b/packages/kit/test/apps/read-file-test/[file].txt
similarity index 100%
rename from packages/kit/test/apps/read-file-test.txt
rename to packages/kit/test/apps/read-file-test/[file].txt