diff --git a/Cargo.lock b/Cargo.lock
index 05a72a7e2..2e6981708 100644
--- a/Cargo.lock
+++ b/Cargo.lock
@@ -2562,6 +2562,16 @@ version = "0.2.9"
 source = "registry+https://github.com/rust-lang/crates.io-index"
 checksum = "28dea519a9695b9977216879a3ebfddf92f1c08c05d984f8996aecd6ecdc811d"
 
+[[package]]
+name = "file-lock"
+version = "2.1.11"
+source = "registry+https://github.com/rust-lang/crates.io-index"
+checksum = "040b48f80a749da50292d0f47a1e2d5bf1d772f52836c07f64bfccc62ba6e664"
+dependencies = [
+ "cc",
+ "libc",
+]
+
 [[package]]
 name = "find_cuda_helper"
 version = "0.2.0"
@@ -5010,6 +5020,7 @@ dependencies = [
  "dirs",
  "env_logger",
  "ethers-core",
+ "file-lock",
  "flate2",
  "hyper 0.14.28",
  "lazy_static",
diff --git a/provers/sgx/setup/Cargo.toml b/provers/sgx/setup/Cargo.toml
index 792ee6bc1..9639c6e1d 100644
--- a/provers/sgx/setup/Cargo.toml
+++ b/provers/sgx/setup/Cargo.toml
@@ -57,6 +57,7 @@ url = { workspace = true }
 cfg-if = { workspace = true }
 cap = { workspace = true }
 dirs = { workspace = true }
+file-lock = "2.1.11"
 
 [dev-dependencies]
 assert_cmd = { workspace = true }
@@ -76,7 +77,4 @@ default = ["sgx"]
 #   "dep:risc0-driver",
 #   "risc0-driver/enable",
 # ]
-sgx = [
-  "dep:sgx-prover",
-  "sgx-prover/enable",
-]
+sgx = ["dep:sgx-prover", "sgx-prover/enable"]
diff --git a/provers/sgx/setup/src/setup_bootstrap.rs b/provers/sgx/setup/src/setup_bootstrap.rs
index 15c134be8..06066ddaa 100644
--- a/provers/sgx/setup/src/setup_bootstrap.rs
+++ b/provers/sgx/setup/src/setup_bootstrap.rs
@@ -7,6 +7,7 @@ use std::{
 
 use crate::app_args::BootstrapArgs;
 use anyhow::{anyhow, Context, Result};
+use file_lock::{FileLock, FileOptions};
 use raiko_lib::consts::SupportedChainSpecs;
 use serde_json::{Number, Value};
 use sgx_prover::{
@@ -20,6 +21,15 @@ pub(crate) async fn setup_bootstrap(
     config_dir: PathBuf,
     bootstrap_args: &BootstrapArgs,
 ) -> Result<()> {
+    // Lock the bootstrap process to prevent multiple instances from running concurrently.
+    // Block until the lock is acquired.
+    // Create the lock file if it does not exist.
+    // Drop the lock file when the lock goes out of scope by drop guard.
+    let _filelock = FileLock::lock(
+        config_dir.join("bootstrap.lock"),
+        true,
+        FileOptions::new().create(true),
+    )?;
     let chain_specs = SupportedChainSpecs::merge_from_file(bootstrap_args.chain_spec_path.clone())?;
     let l1_chain_spec = chain_specs
         .get_chain_spec(&bootstrap_args.l1_network)