diff --git a/executor/aggfuncs/func_count_distinct.go b/executor/aggfuncs/func_count_distinct.go
index 8e73fd2b32ca4..21c9e54115f7d 100644
--- a/executor/aggfuncs/func_count_distinct.go
+++ b/executor/aggfuncs/func_count_distinct.go
@@ -319,6 +319,10 @@ func (e *countOriginalWithDistinct) UpdatePartialResult(sctx sessionctx.Context,
 	p := (*partialResult4CountWithDistinct)(pr)
 
 	encodedBytes := make([]byte, 0)
+	collators := make([]collate.Collator, 0, len(e.args))
+	for _, arg := range e.args {
+		collators = append(collators, collate.GetCollator(arg.GetType().Collate))
+	}
 	// Decimal struct is the biggest type we will use.
 	buf := make([]byte, types.MyDecimalStructSize)
 
@@ -328,7 +332,7 @@ func (e *countOriginalWithDistinct) UpdatePartialResult(sctx sessionctx.Context,
 		encodedBytes = encodedBytes[:0]
 
 		for i := 0; i < len(e.args) && !hasNull; i++ {
-			encodedBytes, isNull, err = evalAndEncode(sctx, e.args[i], row, buf, encodedBytes)
+			encodedBytes, isNull, err = evalAndEncode(sctx, e.args[i], collators[i], row, buf, encodedBytes)
 			if err != nil {
 				return memDelta, err
 			}
@@ -349,7 +353,7 @@ func (e *countOriginalWithDistinct) UpdatePartialResult(sctx sessionctx.Context,
 
 // evalAndEncode eval one row with an expression and encode value to bytes.
 func evalAndEncode(
-	sctx sessionctx.Context, arg expression.Expression,
+	sctx sessionctx.Context, arg expression.Expression, collator collate.Collator,
 	row chunk.Row, buf, encodedBytes []byte,
 ) (_ []byte, isNull bool, err error) {
 	switch tp := arg.GetType().EvalType(); tp {
@@ -401,7 +405,7 @@ func evalAndEncode(
 		if err != nil || isNull {
 			break
 		}
-		encodedBytes = codec.EncodeCompactBytes(encodedBytes, hack.Slice(val))
+		encodedBytes = codec.EncodeCompactBytes(encodedBytes, collator.Key(val))
 	default:
 		return nil, false, errors.Errorf("unsupported column type for encode %d", tp)
 	}
@@ -784,6 +788,10 @@ func (e *approxCountDistinctOriginal) UpdatePartialResult(sctx sessionctx.Contex
 	encodedBytes := make([]byte, 0)
 	// Decimal struct is the biggest type we will use.
 	buf := make([]byte, types.MyDecimalStructSize)
+	collators := make([]collate.Collator, 0, len(e.args))
+	for _, arg := range e.args {
+		collators = append(collators, collate.GetCollator(arg.GetType().Collate))
+	}
 
 	for _, row := range rowsInGroup {
 		var err error
@@ -791,7 +799,7 @@ func (e *approxCountDistinctOriginal) UpdatePartialResult(sctx sessionctx.Contex
 		encodedBytes = encodedBytes[:0]
 
 		for i := 0; i < len(e.args) && !hasNull; i++ {
-			encodedBytes, isNull, err = evalAndEncode(sctx, e.args[i], row, buf, encodedBytes)
+			encodedBytes, isNull, err = evalAndEncode(sctx, e.args[i], collators[i], row, buf, encodedBytes)
 			if err != nil {
 				return memDelta, err
 			}
diff --git a/expression/integration_test.go b/expression/integration_test.go
index 25c5972c0c789..4bef62b16d047 100644
--- a/expression/integration_test.go
+++ b/expression/integration_test.go
@@ -7026,6 +7026,22 @@ func (s *testIntegrationSerialSuite) TestIssue16668(c *C) {
 	tk.MustQuery("select count(distinct(b)) from tx").Check(testkit.Rows("4"))
 }
 
+func (s *testIntegrationSerialSuite) TestIssue27091(c *C) {
+	collate.SetNewCollationEnabledForTest(true)
+	defer collate.SetNewCollationEnabledForTest(false)
+	tk := testkit.NewTestKit(c, s.store)
+	tk.MustExec("use test")
+	tk.MustExec("drop table if exists tx")
+	tk.MustExec("CREATE TABLE `tx` ( `a` int(11) NOT NULL,`b` varchar(5) CHARACTER SET utf8mb4 COLLATE utf8mb4_general_ci DEFAULT NULL, `c` varchar(5) CHARACTER SET utf8mb4 COLLATE utf8mb4_bin DEFAULT NULL)")
+	tk.MustExec("insert into tx values (1, 'a', 'a'), (2, 'A ', 'a '), (3, 'A', 'A'), (4, 'a ', 'A ')")
+	tk.MustQuery("select count(distinct b) from tx").Check(testkit.Rows("1"))
+	tk.MustQuery("select count(distinct c) from tx").Check(testkit.Rows("2"))
+	tk.MustQuery("select count(distinct b, c) from tx where a < 3").Check(testkit.Rows("1"))
+	tk.MustQuery("select approx_count_distinct(b) from tx").Check(testkit.Rows("1"))
+	tk.MustQuery("select approx_count_distinct(c) from tx").Check(testkit.Rows("2"))
+	tk.MustQuery("select approx_count_distinct(b, c) from tx where a < 3").Check(testkit.Rows("1"))
+}
+
 func (s *testIntegrationSerialSuite) TestCollateStringFunction(c *C) {
 	collate.SetNewCollationEnabledForTest(true)
 	defer collate.SetNewCollationEnabledForTest(false)