diff --git a/flow-server/src/main/java/com/vaadin/flow/server/startup/AbstractRouteRegistryInitializer.java b/flow-server/src/main/java/com/vaadin/flow/server/startup/AbstractRouteRegistryInitializer.java
index 151e579f452..048576d5696 100644
--- a/flow-server/src/main/java/com/vaadin/flow/server/startup/AbstractRouteRegistryInitializer.java
+++ b/flow-server/src/main/java/com/vaadin/flow/server/startup/AbstractRouteRegistryInitializer.java
@@ -102,23 +102,27 @@ private void checkForConflictingAnnotations(VaadinContext context,
 
         RouteAlias[] aliases = route.getAnnotationsByType(RouteAlias.class);
         if (aliases.length > 0) {
-            Route routeAnnotation = route.getAnnotation(Route.class);
+            String routePath = RouteUtil.getRoutePath(context, route);
             Map<String, Long> stats = Arrays.stream(aliases)
-                    .map(RouteAlias::value).collect(Collectors.groupingBy(
-                            Function.identity(), Collectors.counting()));
-            if (stats.containsKey(routeAnnotation.value())) {
+                    .map(ann -> RouteUtil.getRouteAliasPath(route, ann))
+                    .collect(Collectors.groupingBy(Function.identity(),
+                            Collectors.counting()));
+            if (stats.containsKey(routePath)) {
                 throw new InvalidRouteConfigurationException(String.format(
-                        "'%s' declares '@%s' and '@%s' with the same path '%s'",
+                        "'%s' declares '@%s' and '@%s' with the same path '%s'. "
+                                + "Make sure paths are different by checking annotation values "
+                                + "and prefixes defined by layouts",
                         route.getCanonicalName(), Route.class.getSimpleName(),
-                        RouteAlias.class.getSimpleName(),
-                        routeAnnotation.value()));
+                        RouteAlias.class.getSimpleName(), routePath));
             }
             String repeatedAliases = stats.entrySet().stream()
                     .filter(e -> e.getValue() > 1).map(Map.Entry::getKey)
                     .collect(Collectors.joining(", "));
             if (!repeatedAliases.isEmpty()) {
                 throw new InvalidRouteConfigurationException(String.format(
-                        "'%s' declares multiple '@%s' with same paths: %s.",
+                        "'%s' declares multiple '@%s' with same paths: %s."
+                                + "Make sure paths are different by checking annotation values "
+                                + "and prefixes defined by layouts.",
                         route.getCanonicalName(),
                         RouteAlias.class.getSimpleName(), repeatedAliases));
             }
diff --git a/flow-server/src/test/java/com/vaadin/flow/server/startup/AbstractRouteRegistryInitializerTest.java b/flow-server/src/test/java/com/vaadin/flow/server/startup/AbstractRouteRegistryInitializerTest.java
index 8734d6ccf21..04acea9127d 100644
--- a/flow-server/src/test/java/com/vaadin/flow/server/startup/AbstractRouteRegistryInitializerTest.java
+++ b/flow-server/src/test/java/com/vaadin/flow/server/startup/AbstractRouteRegistryInitializerTest.java
@@ -20,15 +20,18 @@
 
 import org.junit.Assert;
 import org.junit.Test;
+import org.mockito.Mockito;
 
 import com.vaadin.flow.component.Component;
 import com.vaadin.flow.component.Tag;
 import com.vaadin.flow.router.ParentLayout;
 import com.vaadin.flow.router.Route;
 import com.vaadin.flow.router.RouteAlias;
+import com.vaadin.flow.router.RoutePrefix;
 import com.vaadin.flow.router.RouterLayout;
 import com.vaadin.flow.server.InvalidRouteConfigurationException;
 import com.vaadin.flow.server.InvalidRouteLayoutConfigurationException;
+import com.vaadin.flow.server.VaadinContext;
 
 public class AbstractRouteRegistryInitializerTest {
 
@@ -36,6 +39,8 @@ public class AbstractRouteRegistryInitializerTest {
 
     };
 
+    VaadinContext context = Mockito.mock(VaadinContext.class);
+
     @Tag(Tag.DIV)
     public static class TestParentLayout extends Component
             implements RouterLayout {
@@ -64,6 +69,43 @@ public static class RouteAndAliasWithSamePath extends Component {
 
     }
 
+    @Tag(Tag.DIV)
+    @RoutePrefix("parent")
+    public static class PrefixedParentLayout extends Component
+            implements RouterLayout {
+    }
+
+    @Tag(Tag.DIV)
+    @RoutePrefix("nested")
+    @ParentLayout(PrefixedParentLayout.class)
+    public static class NestedPrefixedParentLayout extends Component
+            implements RouterLayout {
+    }
+
+    @Tag(Tag.DIV)
+    @Route("foo")
+    @RouteAlias(value = "foo", layout = PrefixedParentLayout.class)
+    public static class RouteAndAliasWithSamePathDifferentLayoutPrefix
+            extends Component {
+
+    }
+
+    @Tag(Tag.DIV)
+    @Route(value = "foo", layout = PrefixedParentLayout.class)
+    @RouteAlias(value = "foo", layout = PrefixedParentLayout.class)
+    public static class RouteAndAliasWithSamePathSameLayoutPrefix
+            extends Component {
+
+    }
+
+    @Tag(Tag.DIV)
+    @Route(value = "foo", layout = NestedPrefixedParentLayout.class)
+    @RouteAlias(value = "foo", layout = NestedPrefixedParentLayout.class)
+    public static class RouteAndAliasWithSamePathSameNestedLayoutPrefix
+            extends Component {
+
+    }
+
     @Tag(Tag.DIV)
     @Route("foo")
     @RouteAlias("bar")
@@ -77,7 +119,7 @@ public static class AliasesWithSamePath extends Component {
 
     @Test(expected = InvalidRouteLayoutConfigurationException.class)
     public void routeAndParentLayout_notRouterLayout_throws() {
-        initializer.validateRouteClasses(null,
+        initializer.validateRouteClasses(context,
                 Stream.of(RouteAndParentLayout.class));
     }
 
@@ -85,7 +127,7 @@ public void routeAndParentLayout_notRouterLayout_throws() {
     public void validateRouteClasses_samePathForRouteAndAlias_throws() {
         InvalidRouteConfigurationException exception = Assert.assertThrows(
                 InvalidRouteConfigurationException.class,
-                () -> initializer.validateRouteClasses(null,
+                () -> initializer.validateRouteClasses(context,
                         Stream.of(RouteAndAliasWithSamePath.class)));
         Assert.assertTrue(containsQuotedAnnotationName(exception.getMessage(),
                 Route.class));
@@ -99,7 +141,7 @@ public void validateRouteClasses_samePathForRouteAndAlias_throws() {
     public void validateRouteClasses_samePathForRepeatableAlias_throws() {
         InvalidRouteConfigurationException exception = Assert.assertThrows(
                 InvalidRouteConfigurationException.class,
-                () -> initializer.validateRouteClasses(null,
+                () -> initializer.validateRouteClasses(context,
                         Stream.of(AliasesWithSamePath.class)));
         Assert.assertFalse(containsQuotedAnnotationName(exception.getMessage(),
                 Route.class));
@@ -112,10 +154,49 @@ public void validateRouteClasses_samePathForRepeatableAlias_throws() {
         Assert.assertFalse(exception.getMessage().contains("hey"));
     }
 
+    @Test
+    public void validateRouteClasses_samePathForRouteAndAlias_sameLayoutPrefix_throws() {
+        InvalidRouteConfigurationException exception = Assert.assertThrows(
+                InvalidRouteConfigurationException.class,
+                () -> initializer.validateRouteClasses(context, Stream
+                        .of(RouteAndAliasWithSamePathSameLayoutPrefix.class)));
+        Assert.assertTrue(containsQuotedAnnotationName(exception.getMessage(),
+                Route.class));
+        Assert.assertTrue(containsQuotedAnnotationName(exception.getMessage(),
+                RouteAlias.class));
+        Assert.assertTrue(exception.getMessage().contains("same path"));
+        Assert.assertTrue(exception.getMessage().contains("foo"));
+    }
+
+    @Test
+    public void validateRouteClasses_samePathForRouteAndAlias_sameNestedLayoutPrefix_throws() {
+        InvalidRouteConfigurationException exception = Assert.assertThrows(
+                InvalidRouteConfigurationException.class,
+                () -> initializer.validateRouteClasses(context, Stream.of(
+                        RouteAndAliasWithSamePathSameNestedLayoutPrefix.class)));
+        Assert.assertTrue(containsQuotedAnnotationName(exception.getMessage(),
+                Route.class));
+        Assert.assertTrue(containsQuotedAnnotationName(exception.getMessage(),
+                RouteAlias.class));
+        Assert.assertTrue(exception.getMessage().contains("same path"));
+        Assert.assertTrue(exception.getMessage().contains("foo"));
+    }
+
+    @Test
+    public void validateRouteClasses_samePathForRouteAndAlias_differentLayoutPrefix_doNotThrow() {
+        Set<Class<? extends Component>> classes = initializer
+                .validateRouteClasses(context, Stream.of(
+                        RouteAndAliasWithSamePathDifferentLayoutPrefix.class));
+        Assert.assertEquals(1, classes.size());
+        Assert.assertEquals(
+                RouteAndAliasWithSamePathDifferentLayoutPrefix.class,
+                classes.iterator().next());
+    }
+
     @Test
     public void routeAndParentLayout_routerLayout_returnsValidatedClass() {
         Set<Class<? extends Component>> classes = initializer
-                .validateRouteClasses(null,
+                .validateRouteClasses(context,
                         Stream.of(RouteAndParentRouterLayout.class));
         Assert.assertEquals(1, classes.size());
         Assert.assertEquals(RouteAndParentRouterLayout.class,