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<title>Contributing to seq2science &mdash; seq2science documentation</title>
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Expand Up @@ -9,6 +9,12 @@ All changed fall under either one of these types: `Added`, `Changed`, `Deprecate

## [Unreleased]

### Fixed

- chipseeker env got corrupted, it should work again.
- replaced deprecated --split-e flag with --split-3 flag for fastq downloading
- removed support for GSA as their "API" changed

## [1.2.1] - 2023-11-15

### Fixed
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Expand Up @@ -9,7 +9,7 @@ Downloading public data in bulk from the NCBI, ENA, and DDBJ databases has never

#### Download SRA file

The five most popular databases that store sequencing data are National Center for Biotechnology Information (NCBI), the European Nucleotide Archive (ENA), the DNA Data Bank of Japan (DDBJ), the Genome Sequence Archive (GSA), and the Encode project (ENCODE).
The five most popular databases that store sequencing data are National Center for Biotechnology Information (NCBI), the European Nucleotide Archive (ENA), the DNA Data Bank of Japan (DDBJ), the Genome Sequence Archive (GSA) (GSA is currently not supported anymore), and the Encode project (ENCODE).
ENA, ENCODE, and GSA store the actual fastq files, and DDBJ and NCBI store the raw data (as a sra file) from which a fastq can be derived.
For this reason for each sample on DDBJ and NCBI seq2science will first check if it can be downloaded from ENA as a fastq directly.
Otherwise we will download the samples in its raw format. To convert this data to a fastq it has to be "*dumped*".
Expand All @@ -22,7 +22,6 @@ As an example, the `samples.tsv` could look something like this:

```
sample
CRX123 <-- GSA experiment
DRX890 <-- DDBJ experiment
DRR098 <-- DDBJ run
ENCSR765 <-- ENCODE assay
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<title>Differential gene/peak analysis &mdash; seq2science documentation</title>
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16 changes: 9 additions & 7 deletions content/all_rules.html
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<title>Per rule explanation &mdash; seq2science documentation</title>
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Expand Down Expand Up @@ -647,15 +649,15 @@ <h2>featureCounts<a class="headerlink" href="#featurecounts" title="Link to this
mv $(dirname {output.real_out})/$(basename {output.summary}) {output.summary}
</pre></div>
</div>
<p><a class="reference external" href="https://github.com/vanheeringen-lab/seq2science/blob/docs_updates/seq2science/envs/gene_counts.yaml">featureCounts environment</a>.</p>
<p><a class="reference external" href="https://github.com/vanheeringen-lab/seq2science/blob/docs_updates/seq2science/envs/subread.yaml">featureCounts environment</a>.</p>
</section>
<section id="id1">
<h2>featurecounts<a class="headerlink" href="#id1" title="Link to this heading"></a></h2>
<p>summarize reads to gene level. Outputs a counts table per bam file.</p>
<div class="highlight-default notranslate"><div class="highlight"><pre><span></span><span class="n">featureCounts</span> <span class="o">-</span><span class="n">a</span> <span class="p">{</span><span class="nb">input</span><span class="o">.</span><span class="n">gtf</span><span class="p">}</span> <span class="p">{</span><span class="nb">input</span><span class="o">.</span><span class="n">bam</span><span class="p">}</span> <span class="p">{</span><span class="n">params</span><span class="o">.</span><span class="n">endedness</span><span class="p">}</span> <span class="o">-</span><span class="n">s</span> <span class="p">{</span><span class="n">params</span><span class="o">.</span><span class="n">strandedness</span><span class="p">}</span> <span class="p">{</span><span class="n">params</span><span class="o">.</span><span class="n">user_flags</span><span class="p">}</span> <span class="o">-</span><span class="n">T</span> <span class="p">{</span><span class="n">threads</span><span class="p">}</span> <span class="o">-</span><span class="n">o</span> <span class="p">{</span><span class="n">output</span><span class="p">}</span> <span class="o">&gt;</span> <span class="p">{</span><span class="n">log</span><span class="p">}</span> <span class="mi">2</span><span class="o">&gt;&amp;</span><span class="mi">1</span>
</pre></div>
</div>
<p><a class="reference external" href="https://github.com/vanheeringen-lab/seq2science/blob/docs_updates/seq2science/envs/gene_counts.yaml">featurecounts environment</a>.</p>
<p><a class="reference external" href="https://github.com/vanheeringen-lab/seq2science/blob/docs_updates/seq2science/envs/subread.yaml">featurecounts environment</a>.</p>
</section>
<section id="full-decoy-transcripts">
<h2>full_decoy_transcripts<a class="headerlink" href="#full-decoy-transcripts" title="Link to this heading"></a></h2>
Expand Down Expand Up @@ -1407,7 +1409,7 @@ <h2>sra2fastq_PE<a class="headerlink" href="#sra2fastq-pe" title="Link to this h

# dump to tmp dir
parallel-fastq-dump -s {input} -O {output.tmpdir} \
--threads {threads} --split-e --skip-technical --dumpbase \
--threads {threads} --split-3 --skip-technical --dumpbase \
--readids --clip --read-filter pass --defline-seq &#39;@$ac.$si.$sg/$ri&#39; \
--defline-qual &#39;+&#39; --gzip &gt;&gt; {log} 2&gt;&amp;1

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Expand Down Expand Up @@ -106,7 +108,7 @@
<h1>Command line interface<a class="headerlink" href="#command-line-interface" title="Link to this heading"></a></h1>
<section id="init">
<h2>init<a class="headerlink" href="#init" title="Link to this heading"></a></h2>
<p><p>Each workflow requires a configuration and samples file to run. Running “seq2science init {workflow}” initialises a default configuration and samples file for the specific workflow. Supported workflows: alignment, download-fastq, scrna-seq, atac-seq, scatac-seq, chip-seq, rna-seq</p>
<p><p>Each workflow requires a configuration and samples file to run. Running “seq2science init {workflow}” initialises a default configuration and samples file for the specific workflow. Supported workflows: alignment, scrna-seq, scatac-seq, atac-seq, rna-seq, download-fastq, chip-seq</p>
</p>
<div class="highlight-default notranslate"><div class="highlight"><pre><span></span><span class="n">usage</span><span class="p">:</span> <span class="n">seq2science</span> <span class="n">init</span> <span class="p">[</span><span class="o">-</span><span class="n">h</span><span class="p">]</span> <span class="p">[</span><span class="o">--</span><span class="nb">dir</span> <span class="n">PATH</span><span class="p">]</span> <span class="p">[</span><span class="o">-</span><span class="n">f</span><span class="p">]</span> <span class="n">WORKFLOW</span>
</pre></div>
Expand All @@ -115,7 +117,7 @@ <h2>init<a class="headerlink" href="#init" title="Link to this heading"></a><
<h3>Positional Arguments<a class="headerlink" href="#positional-arguments" title="Link to this heading"></a></h3>
<dl class="option-list">
<dt><kbd>WORKFLOW</kbd></dt>
<dd><p>Possible choices: alignment, download-fastq, scrna-seq, atac-seq, scatac-seq, chip-seq, rna-seq</p>
<dd><p>Possible choices: alignment, scrna-seq, scatac-seq, atac-seq, rna-seq, download-fastq, chip-seq</p>
</dd>
</dl>
</section>
Expand All @@ -136,7 +138,7 @@ <h3>Named Arguments<a class="headerlink" href="#named-arguments" title="Link to
<hr class="docutils" />
<section id="run">
<h2>run<a class="headerlink" href="#run" title="Link to this heading"></a></h2>
<p><p>Run a complete workflow. This requires that a config and samples file are either present in the current directory, or passed as an argument. Supported workflows: alignment, download-fastq, scrna-seq, atac-seq, scatac-seq, chip-seq, rna-seq</p>
<p><p>Run a complete workflow. This requires that a config and samples file are either present in the current directory, or passed as an argument. Supported workflows: alignment, scrna-seq, scatac-seq, atac-seq, rna-seq, download-fastq, chip-seq</p>
</p>
<div class="highlight-default notranslate"><div class="highlight"><pre><span></span><span class="n">usage</span><span class="p">:</span> <span class="n">seq2science</span> <span class="n">run</span> <span class="p">[</span><span class="o">-</span><span class="n">h</span><span class="p">]</span> <span class="p">[</span><span class="o">-</span><span class="n">j</span> <span class="n">N</span><span class="p">]</span> <span class="p">[</span><span class="o">-</span><span class="n">n</span><span class="p">]</span> <span class="p">[</span><span class="o">-</span><span class="n">r</span><span class="p">]</span> <span class="p">[</span><span class="o">--</span><span class="n">skip</span><span class="o">-</span><span class="n">rerun</span><span class="p">]</span> <span class="p">[</span><span class="o">-</span><span class="n">k</span><span class="p">]</span>
<span class="p">[</span><span class="o">--</span><span class="n">rerun</span><span class="o">-</span><span class="n">incomplete</span><span class="p">]</span> <span class="p">[</span><span class="o">--</span><span class="n">unlock</span><span class="p">]</span>
Expand All @@ -150,7 +152,7 @@ <h2>run<a class="headerlink" href="#run" title="Link to this heading"></a></h
<h3>Positional Arguments<a class="headerlink" href="#positional-arguments" title="Link to this heading"></a></h3>
<dl class="option-list">
<dt><kbd>WORKFLOW</kbd></dt>
<dd><p>Possible choices: alignment, download-fastq, scrna-seq, atac-seq, scatac-seq, chip-seq, rna-seq</p>
<dd><p>Possible choices: alignment, scrna-seq, scatac-seq, atac-seq, rna-seq, download-fastq, chip-seq</p>
</dd>
</dl>
</section>
Expand Down Expand Up @@ -217,7 +219,7 @@ <h2>clean<a class="headerlink" href="#clean" title="Link to this heading"></a
<hr class="docutils" />
<section id="explain">
<h2>explain<a class="headerlink" href="#explain" title="Link to this heading"></a></h2>
<p><p>Explains what has/will be done for the workflow. This prints a string which can serve as a skeleton for your material &amp; methods section. Supported workflows: alignment, download-fastq, scrna-seq, atac-seq, scatac-seq, chip-seq, rna-seq</p>
<p><p>Explains what has/will be done for the workflow. This prints a string which can serve as a skeleton for your material &amp; methods section. Supported workflows: alignment, scrna-seq, scatac-seq, atac-seq, rna-seq, download-fastq, chip-seq</p>
</p>
<div class="highlight-default notranslate"><div class="highlight"><pre><span></span><span class="n">usage</span><span class="p">:</span> <span class="n">seq2science</span> <span class="n">explain</span> <span class="p">[</span><span class="o">-</span><span class="n">h</span><span class="p">]</span> <span class="p">[</span><span class="o">--</span><span class="n">hyperref</span><span class="p">]</span>
<span class="p">[</span><span class="o">--</span><span class="n">snakemakeOptions</span> <span class="n">KEY</span><span class="o">=</span><span class="n">VAL</span> <span class="p">[</span><span class="n">KEY</span><span class="o">=</span><span class="n">VAL</span> <span class="o">...</span><span class="p">]]</span>
Expand All @@ -229,7 +231,7 @@ <h2>explain<a class="headerlink" href="#explain" title="Link to this heading">
<h3>Positional Arguments<a class="headerlink" href="#positional-arguments" title="Link to this heading"></a></h3>
<dl class="option-list">
<dt><kbd>WORKFLOW</kbd></dt>
<dd><p>Possible choices: alignment, download-fastq, scrna-seq, atac-seq, scatac-seq, chip-seq, rna-seq</p>
<dd><p>Possible choices: alignment, scrna-seq, scatac-seq, atac-seq, rna-seq, download-fastq, chip-seq</p>
</dd>
</dl>
</section>
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