diff --git a/packages/vite/src/node/plugins/assetImportMetaUrl.ts b/packages/vite/src/node/plugins/assetImportMetaUrl.ts
index 6872de5249e014..d16b1ad7540b64 100644
--- a/packages/vite/src/node/plugins/assetImportMetaUrl.ts
+++ b/packages/vite/src/node/plugins/assetImportMetaUrl.ts
@@ -5,6 +5,7 @@ import type { Plugin } from '../plugin'
 import type { ResolvedConfig } from '../config'
 import type { ResolveFn } from '../'
 import {
+  injectQuery,
   isParentDirectory,
   normalizePath,
   slash,
@@ -55,7 +56,12 @@ export function assetImportMetaUrlPlugin(config: ResolvedConfig): Plugin {
 
           // potential dynamic template string
           if (rawUrl[0] === '`' && rawUrl.includes('${')) {
-            const ast = this.parse(rawUrl)
+            let [pureUrl, queryString = ''] = rawUrl.split('?')
+            if (queryString) {
+              pureUrl += '`'
+              queryString = '?' + queryString.slice(0, -1)
+            }
+            const ast = this.parse(pureUrl)
             const templateLiteral = (ast as any).body[0].expression
             if (templateLiteral.expressions.length) {
               const pattern = buildGlobPattern(templateLiteral)
@@ -65,6 +71,12 @@ export function assetImportMetaUrlPlugin(config: ResolvedConfig): Plugin {
                 continue
               }
 
+              const globOptions = {
+                eager: true,
+                import: 'default',
+                // A hack to allow 'as' & 'query' exist at the same time
+                query: injectQuery(queryString, 'url'),
+              }
               // Note: native import.meta.url is not supported in the baseline
               // target so we use the global location here. It can be
               // window.location or self.location in case it is used in a Web Worker.
@@ -74,7 +86,9 @@ export function assetImportMetaUrlPlugin(config: ResolvedConfig): Plugin {
                 index + exp.length,
                 `new URL((import.meta.glob(${JSON.stringify(
                   pattern,
-                )}, { eager: true, import: 'default', as: 'url' }))[${rawUrl}], self.location)`,
+                )}, ${JSON.stringify(
+                  globOptions,
+                )}))[${pureUrl}], self.location)`,
               )
               continue
             }
diff --git a/playground/assets/__tests__/assets.spec.ts b/playground/assets/__tests__/assets.spec.ts
index 3aa3a9ab3189dd..29d56820c6c4bf 100644
--- a/playground/assets/__tests__/assets.spec.ts
+++ b/playground/assets/__tests__/assets.spec.ts
@@ -324,6 +324,17 @@ test('new URL(`${dynamic}`, import.meta.url)', async () => {
   )
 })
 
+test('new URL(`./${dynamic}?abc`, import.meta.url)', async () => {
+  expect(await page.textContent('.dynamic-import-meta-url-1-query')).toMatch(
+    isBuild ? 'data:image/png;base64' : '/foo/nested/icon.png?abc',
+  )
+  expect(await page.textContent('.dynamic-import-meta-url-2-query')).toMatch(
+    isBuild
+      ? /\/foo\/assets\/asset-\w{8}\.png\?abc/
+      : '/foo/nested/asset.png?abc',
+  )
+})
+
 test('new URL(`non-existent`, import.meta.url)', async () => {
   expect(await page.textContent('.non-existent-import-meta-url')).toMatch(
     new URL('non-existent', page.url()).pathname,
diff --git a/playground/assets/index.html b/playground/assets/index.html
index 57caa90f4f552d..b9e857398b6c35 100644
--- a/playground/assets/index.html
+++ b/playground/assets/index.html
@@ -221,6 +221,16 @@ <h2>new URL(`./${dynamic}`, import.meta.url,) (with comma)</h2>
   <code class="dynamic-import-meta-url-2-comma"></code>
 </p>
 
+<h2>new URL(`./${dynamic}?abc`, import.meta.url)</h2>
+<p>
+  <img class="dynamic-import-meta-url-img-1-query" />
+  <code class="dynamic-import-meta-url-1-query"></code>
+</p>
+<p>
+  <img class="dynamic-import-meta-url-img-2-query" />
+  <code class="dynamic-import-meta-url-2-query"></code>
+</p>
+
 <h2>new URL(`non-existent`, import.meta.url)</h2>
 <p>
   <code class="non-existent-import-meta-url"></code>
@@ -432,6 +442,17 @@ <h3>assets in noscript</h3>
   testDynamicImportMetaUrlWithComma('icon', 1)
   testDynamicImportMetaUrlWithComma('asset', 2)
 
+  function testDynamicImportMetaUrlWithQuery(name, i) {
+    // prettier-ignore
+    const metaUrl = new URL(`./nested/${name}.png?abc`, import.meta.url,)
+    text(`.dynamic-import-meta-url-${i}-query`, metaUrl)
+    document.querySelector(`.dynamic-import-meta-url-img-${i}-query`).src =
+      metaUrl
+  }
+
+  testDynamicImportMetaUrlWithQuery('icon', 1)
+  testDynamicImportMetaUrlWithQuery('asset', 2)
+
   {
     const name = 'test'
     const js = new URL(`./nested/${name}.js`, import.meta.url).href