diff --git a/packages/vite/src/node/server/sourcemap.ts b/packages/vite/src/node/server/sourcemap.ts
index 741ae4331ade22..684dff128e597d 100644
--- a/packages/vite/src/node/server/sourcemap.ts
+++ b/packages/vite/src/node/server/sourcemap.ts
@@ -4,6 +4,7 @@ import convertSourceMap from 'convert-source-map'
 import type { ExistingRawSourceMap, SourceMap } from 'rollup'
 import type { Logger } from '../logger'
 import { blankReplacer, createDebugger } from '../utils'
+import { cleanUrl } from '../../shared/utils'
 
 const debug = createDebugger('vite:sourcemap', {
   onlyWhenFocused: true,
@@ -53,7 +54,7 @@ export async function injectSourcesContent(
           // inject content from source file when sourcesContent is null
           sourceRootPromise ??= computeSourceRoute(map, file)
           const sourceRoot = await sourceRootPromise
-          let resolvedSourcePath = decodeURI(sourcePath)
+          let resolvedSourcePath = cleanUrl(decodeURI(sourcePath))
           if (sourceRoot) {
             resolvedSourcePath = path.resolve(sourceRoot, resolvedSourcePath)
           }