This repository provides R Markdown scripts (.Rmd) and corresponding HTML reports with the post-quantification analyses of the data presented in the main paper for the first large-scale multi-omic multi-tissue endurance exercise training study conducted in young adult rats by the Molecular Transducers of Physical Activity Consortium (MoTrPAC). Find the preprint on bioRxiv.
Each omic data generated for the paper above had a pipeline to generate quantified features from the raw data (e.g., gene-level counts for transcriptomics data quantified from raw RNA-seq reads). In this repository we provide scripts with the initial analyses of these post-quantification data, which are exploratory in nature. These analyses were used for sanity checks, QA procedures, and identifying flagged samples such as extreme PCA outliers.
The reports provided in this repository were originally intended for use within MoTrPAC. Therefore, some functionalities that fetch data directly from the internal MoTrPAC cloud systems are not expected to work as-is. Nevertheless, we decided to provide these reports because: (1) they contain useful code/analyses and figures (e.g., PC plots of each tissue in the transcriptomics report), and (2) most analyses can be reproduced by downloading the data from the MoTrPAC Data Hub and modifying the code to take the data from the local directories to which the data were downloaded. The relevant code sections and paths in each report (and its Rmd file) typically use ADDPATH as a token that replaced internal MoTrPAC cloud paths.
MoTrPAC is a national research consortium designed to discover and perform preliminary characterization of the range of molecular transducers (the "molecular map") that underlie the health benefits of physical activity in humans. The program's goal is to study the molecular changes that occur during and after exercise and ultimately to advance the understanding of how physical activity improves and preserves health. The six-year program is the largest targeted NIH investment of funds into the mechanisms of how physical activity improves health and prevents disease. See motrpac.org and motrpac-data.org for more details.
This repo is organized as follows:
tools- functions, can source each.Rfile into a sessionreports- the main folder with the reports (.Rmdfiles and the corresponding HTML reports)ext_data- additional data required for reproducing some analyses (e.g., genome.gtf for the ATAC-seq analysis)
NOTE: These links will work when the repository is made public.
The HTML reports can be viewed either by downloading this repository and opening the HTML files or by clicking the following links.