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Added example of queuing multiple jobs to torque
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#!/bin/bash | ||
base_dir=/path/to/project/ | ||
ref=$base_dir/ref/mm10.fa | ||
working_dir=$base_dir/working | ||
inputs="$base_dir/input1.bam $base_dir/input2.bam $base_dir/input3.bam" | ||
inputarr=($inputs) | ||
sample_name=example_torque | ||
job_prefix=gridss_${sample_name} | ||
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mkdir -p $working_dir | ||
cat > $working_dir/gridss.properties << EOF | ||
# The default chunk size of 10Mb only gives ~300 units of work | ||
# reducing to 1Mb gives ~3000 which gives a more even distribution | ||
# of work across jobs | ||
chunkSize=1000000 | ||
chunkSequenceChangePenalty=100000 | ||
EOF | ||
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# Assumes gridss.sh release artifacts are in $base_dir | ||
gridss_cmd_common="$base_dir/gridss.sh \ | ||
-r $ref \ | ||
-o $base_dir/$sample_name.sv.vcf \ | ||
-a $base_dir/$sample_name.asm.bam \ | ||
-j $base_dir/gridss-2.6.3-gridss-jar-with-dependencies.jar \ | ||
-w $working_dir \ | ||
-c $working_dir/gridss.properties" | ||
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# Create a job array for each input file | ||
threads=8 | ||
cat > $working_dir/${job_prefix}_preprocess.sh << EOF | ||
#!/bin/bash | ||
#PBS -N ${job_prefix}_preprocess | ||
##PBS -o ${job_prefix}_preprocess | ||
#PBS -t 1-${#inputarr[@]} | ||
#PBS -l nodes=1:ppn=$threads,mem=16gb,walltime=144:00:0 | ||
#PBS -j oe | ||
module add bwa samtools R sambamba java | ||
inputarr=($inputs) | ||
cd $working_dir | ||
$gridss_cmd_common -t $threads \ | ||
-s preprocess \ | ||
\${inputarr[\$((PBS_ARRAYID -1 ))]} | ||
EOF | ||
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# Create a job array containing assembly jobs | ||
assembly_jobs=32 | ||
cat > $working_dir/${job_prefix}_assembly.sh << EOF | ||
#!/bin/bash | ||
#PBS -N ${job_prefix}_assembly | ||
##PBS -o ${job_prefix}_assembly | ||
#PBS -t 1-$assembly_jobs | ||
#PBS -l nodes=1:ppn=$threads,mem=30gb,walltime=144:00:0 | ||
#PBS -j oe | ||
module add bwa samtools R sambamba java | ||
inputarr=($inputs) | ||
cd $working_dir | ||
$gridss_cmd_common -t $threads \ | ||
-s assemble \ | ||
--jobindex \$((PBS_ARRAYID -1 )) \ | ||
--jobnodes $assembly_jobs \ | ||
$inputs | ||
EOF | ||
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# And finally, finish perform assembly gather | ||
# variant calling, and annotation | ||
threads=16 | ||
cat > $working_dir/${job_prefix}_call.sh << EOF | ||
#!/bin/bash | ||
#PBS -N ${job_prefix}_call | ||
##PBS -o ${job_prefix}_call | ||
#PBS -l nodes=1:ppn=$threads,mem=30gb,walltime=144:00:0 | ||
#PBS -j oe | ||
module add bwa samtools R sambamba java | ||
inputarr=($inputs) | ||
cd $working_dir | ||
$gridss_cmd_common -t $threads \ | ||
$inputs | ||
EOF | ||
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# queue the jobs up with a job dependency on the previous step | ||
echo "pbs_id=\$(qsub $working_dir/${job_prefix}_preprocess.sh) ; echo \$pbs_id" | ||
echo "pbs_id=\$(qsub -W depend=afterokarray:\$pbs_id $working_dir/${job_prefix}_assembly.sh) ; echo \$pbs_id)" | ||
echo "qsub -W depend=afterokarray:\$pbs_id $working_dir/${job_prefix}_call.sh" | ||
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