breseq v0.32.0
jeffreybarrick
released this
20 Jan 20:12
·
296 commits
to master
since this release
- If a mutation overlaps multiple genes, its effect on each gene is now annotated.
(Previously only the first gene encountered was annotated.) The relevant Genome Diff
fields use a semicolon to separate the information about each impacted gene. - New output of summary information and settings in JSON format in data directory file
summary.json
. - Corrected case in polymorphism mode where failing consensus mutations could be
incorrectly predicted if a polymorphism was rejected. More thorough and consistent
output of rejection information for RA evidence. - Updates to VCF output.
- Fixed problem reading some FASTQ files (discovered on Nanopore data).
- More granular options to filter predictions on the coverage of reads supporting them.
For example:
--polymorphism-minimum-variant-coverage
--polymorphism-minimum-total-coverage
--polymorphism-minimum-variant-coverage-each-strand
--polymorphism-minimum-total-coverage-each-strand
WARNING: Previous versions of these options have been renamed for consistency. - New options to customize bowtie2 options used for alignment. (Only intended for expert users!)
--bowtie2-scoring
,--bowtie2-stage1
,--bowtie2-stage2
,--bowtie2-junction
- Removed extra slash in paths to invoke R scripts.
- Removed split alignment pieces that were completely soft-padded in output BAM file.
- C++11 is now required for compilation. Optimization added to default compiler settings.
- Fixed compatibility issues when compiling with certain versions of GCC.