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breseq v0.32.0

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@jeffreybarrick jeffreybarrick released this 20 Jan 20:12
· 296 commits to master since this release
  • If a mutation overlaps multiple genes, its effect on each gene is now annotated.
    (Previously only the first gene encountered was annotated.) The relevant Genome Diff
    fields use a semicolon to separate the information about each impacted gene.
  • New output of summary information and settings in JSON format in data directory file
    summary.json.
  • Corrected case in polymorphism mode where failing consensus mutations could be
    incorrectly predicted if a polymorphism was rejected. More thorough and consistent
    output of rejection information for RA evidence.
  • Updates to VCF output.
  • Fixed problem reading some FASTQ files (discovered on Nanopore data).
  • More granular options to filter predictions on the coverage of reads supporting them.
    For example:
    --polymorphism-minimum-variant-coverage
    --polymorphism-minimum-total-coverage
    --polymorphism-minimum-variant-coverage-each-strand
    --polymorphism-minimum-total-coverage-each-strand
    WARNING: Previous versions of these options have been renamed for consistency.
  • New options to customize bowtie2 options used for alignment. (Only intended for expert users!)
    --bowtie2-scoring, --bowtie2-stage1, --bowtie2-stage2, --bowtie2-junction
  • Removed extra slash in paths to invoke R scripts.
  • Removed split alignment pieces that were completely soft-padded in output BAM file.
  • C++11 is now required for compilation. Optimization added to default compiler settings.
  • Fixed compatibility issues when compiling with certain versions of GCC.