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Analysis of Ssp4 and Ssp6 effects on Pseudomonas fluorescens using Tn-seq (Coulthurst)

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Analysis of Ssp4 and Ssp6 effects on Pseudomonas fluorescens using Tn-seq

The R code to accompany the manuscript "Two ion-selective pore forming effectors delivered by the same Type VI secretion system have distinct consequences for targeted bacterial cells" by Mark Reglinski, Quenton W Hurst, David Williams, Marek Gierlinski, Katharine Mathers, Adam Ostrowski, Megan Bergkessel, Ulrich Zachariae, Samantha J Pitt and Sarah J Coulthurst.

Instructions

The R code in this repository uses renv and targets for reproducibility. renv is used to reproduce the R environment:

install.packages("renv")
renv::restore()

Next, the targets pipeline is invoked with the following command:

targets::tar_make()

Finally, the HTML report, including all figures, can be created with:

quarto::quarto_render("doc/report.qmd")

The HTML file will be created in folder doc.

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Analysis of Ssp4 and Ssp6 effects on Pseudomonas fluorescens using Tn-seq (Coulthurst)

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