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gtf -strand convert to + strand #45

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zlc289 opened this issue Apr 22, 2021 · 2 comments
Open

gtf -strand convert to + strand #45

zlc289 opened this issue Apr 22, 2021 · 2 comments

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@zlc289
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zlc289 commented Apr 22, 2021

Hi, I have a problem when I convert my gtf file to + strand file. I do not what length I should enter. Total length of reference gene or whole length of genome. Because my gtf file is download for whole genome. when I enter reference gene length, the location in + strand file is minus "-". Here is my gtf file. Thanks.
AT5G13480_2_cds.txt
@singing-scientist
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Greetings! Thanks for using SNPGenie. The total length of the FASTA (i.e. the whole reference sequence, whether it is a contig, chromosome, or genome) should be used. Let me know if that helps!

@zlc289
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zlc289 commented Apr 22, 2021 via email

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@singing-scientist @zlc289