Skip to content

Commit

Permalink
Fix documentation
Browse files Browse the repository at this point in the history
  • Loading branch information
rcannood committed May 3, 2023
1 parent a469fc1 commit f639468
Showing 1 changed file with 4 additions and 5 deletions.
9 changes: 4 additions & 5 deletions src/mapping/process_smartseq2/auto.vsh.yaml
Original file line number Diff line number Diff line change
Expand Up @@ -11,11 +11,10 @@ functionality:
* Extract the sample id from the path name using the same `fastq_regex` and `sample_id_replacement`.
* Detect cell ids from the path name using the `fastq_regex` and `cell_id_replacement`.
* Write the list of samples as a yaml in the output directory.
* Map the reads using Cell Ranger
* Convert the Cell Ranger output to h5mu
* Remove ambient RNA with CellBender
* Remove cells with less than 100 genes or 1000 reads.
* Make the cell names unique
* Map the reads using Star
* Sort counts with samtools
* Convert to count table with HTSeq
* Compute QC metrics with MultiQC
* Output one h5mu file per sample
Concatenating the invididual h5mu files into one h5mu file is a separate pipeline.
Expand Down

0 comments on commit f639468

Please sign in to comment.