EMPIAR depositor

Command line tool for depositing data into Electron Microscopy Public Image Archive.

How to use

Please follow these steps:

1. Create a JSON file according to the schema. An example of such a file.

2. Download and install ascp tool and/or install globus-cli (supported version 1.7.0) with

pip install globus-cli==1.7.0

Globus can be used as a separate upload option or as a fallback if Aspera fails.

3. Set the environmental variable for EMPIAR transfer password to the one that EMPIAR team has provided you with. Please note that this is not the API token from 1) and is a separate password from the one that you create when registering EMPIAR user.

• On Linux and Mac OS X execute

  export EMPIAR_TRANSFER_PASS=<empiar_transfer_password>

• On Windows execute

  set EMPIAR_TRANSFER_PASS=<empiar_transfer_password>

4. Run the script as:

   empiar-depositor [-h] [-a ASCP] [-g GLOBUS] [-f] [-e ENTRY_THUMBNAIL] [-r ENTRY_ID ENTRY_DIR] [-i] [-v] EMPIAR_TOKEN JSON_INPUT DATA

Positional arguments:

EMPIAR_TOKEN

EMPIAR API token. You can generate it at https://empiar.org/deposition/api_token. Alternatively, instead of the token you can use your EMPIAR username and provide your password with -p optional argument (see below for more information).

JSON_INPUT

The location of the JSON with EMPIAR deposition information.

DATA

The location of the data that you would like to upload to EMPIAR. It should contain directories that correspond to the image set directories specified in the JSON file.

Optional arguments:

-h, --help

Show help message and exit

-p PASSWORD, --password PASSWORD

Use basic authentication (username + password) instead of token authentication. If no password is provided for this argument, then the user is prompted for a password.

-a ASCP, --ascp ASCP

The location of the ascp executable. By default it is installed in ~/.aspera/connect/bin directory on Linux machines, in ~/Applications/Aspera\ Connect.app/Contents/Resources directory on Macs and in C:\Users\<username>\AppData\Local\Programs\Aspera\Aspera Connect\bin on Windows.

-g GLOBUS, --globus GLOBUS

Use Globus if Aspera is not specified or Aspera transfer fails. Requirement: globus-cli installed and an endpoint created. Specify your unique user identifier (UUID) as the input parameter.

-f, --globus-force-login

Force login to Globus. Login even if the globus-cli already has valid login credentials. Any existing credentials will be removed from local storage and globally revoked.

-e ENTRY_THUMBNAIL, --entry-thumbnail ENTRY_THUMBNAIL

Thumbnail image that will represent your deposition on EMPIAR pages. Minimum size is 400 x 400, preferred format is png. If none is provided, then the image from the related EMDB entry will be used.

-r ENTRY_ID ENTRY_DIR, --resume ENTRY_ID ENTRY_DIR

Resume Aspera upload or re-deposit an entry. The entry has to be successfully created as specifying EMPIAR entry ID and entry directory is required. All entry metadata will be replaced with the one provided in the JSON file. Aspera transfer will continue from where it stopped.

-gu USERNAME_RIGHTS, --grant-rights-usernames USERNAME_RIGHTS

-ge EMAIL_RIGHTS, --grant-rights-emails EMAIL_RIGHTS

-gu ORCID_RIGHTS, --grant-rights-usernames ORCID_RIGHTS

Grant rights based on usernames, emails or ORCiDs. USERNAME_RIGHTS, EMAIL_RIGHTS and ORCID_RIGHTS are comma separated lists of usernames, emails, ORCiDs and rights in format username:rights, email:rights and orcid:rights. Rights can be 1 - Owner, 2 - View only, 3 - View and Edit, 4 - View, Edit and Submit. There can be only one deposition owner.

-i, --ignore-certificate

Activate this flag to skip the verification of SSL certificate.

-v, --version

Show program's version number and exit

Examples:

empiar-depositor -a ~/Applications/Aspera\ Connect.app/Contents/Resources/ascp 0123456789 ~/Documents/empiar_deposition_1.json ~/Downloads/micrographs

empiar-depositor -a ~/Applications/Aspera\ Connect.app/Contents/Resources/ascp 0123456789 ~/Documents/empiar_deposition_1.json ~/Downloads/micrographs -gu johndoe:1,jamessmith:3

empiar-depositor -a ~/Applications/Aspera\ Connect.app/Contents/Resources/ascp 0123456789 ~/Documents/empiar_deposition_1.json ~/Downloads/micrographs -gu johndoe:4,jamessmith:1 -ge jeremycarpenter@email.com:3 -go 0000-0000-0000-0001:2,0000-0000-1000-0002:4

empiar-depositor -r 10 ABC123 -e ~/Downloads/dep_thumb.png 0123456789 -g 01234567-89a-bcde-fghi-jklmnopqrstu ~/Documents/empiar_deposition_1.json ~/Downloads/micrographs

empiar-depositor -a ~/Applications/Aspera\ Connect.app/Contents/Resources/ascp my_empiar_user -p my_empiar_password ~/Documents/empiar_deposition_1.json ~/Downloads/micrographs