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Holmos - Hardware

Holmos is a digital holographic microscope. The software repository has more information about the project.

This repository contains instructions to build your own copy of the setup, and files describing the 3D-printed parts.

Unfortunately, shipping or selling kits is too complicated for our institute, so we cannot offer kits.

Requirements

Purchased parts

See shopping list. The steel rods need to be at least 600 mm long if you are using the reference design.

Download; what to print

Unless you want to modify things, you can download .STL files directly from the reference_assembly subfolder here. Your printer's software should be able to read them directly. Print one of each file, and you'll have everything you need.

If you are using parts with different diameters than the reference_assembly, modify the printable parts accordingly.

We developed this using Ultimaker printers and their Cura software, but any other printer should work.

Assembly

Start by looking at reference_assembly.py. It contains a list of parts, and their z positions along the optical axis. Run the file, and it will generate a scad file showing the entire assembly (except rails). In addition, a folder full of the individual parts is generated. Print those parts, and assemble them at the positions given, and you should have a good starting point.

For instructions on individual parts, e.g. how to mount purchased parts in the printed mounts, see the instructions folder.

Adjustment, Usage

See instructions/adjustment.md in this repository, or browse the German student documentation

Optics Design

instructions/optics_design.md explains the optics design in some more detail. You can probably skip this if you just want to use the microscope.

Optional parts

Stability

Additional struts and crosses (e.g. Rpi_mount) can make the cage more stable.

Light shield

A round_mounts.round_mount_light(20, opening_angle=0, cyl_length=40, ring_thick=2) can help shield the camera against stray light and make the setup work better in bright environments.

Transport

The cage-based setup fits inside a tube (HT DN90). To keep the setup from hitting the tube sides, print some cage.cage_circumference() In a DN90 tube, the Raspberry Pi needs to be removed, and the movable mirror needs to be removed from the crane. It can be stored in a crane_mirror(mirror_offset_x=0, crane_only=True) for transport.

Tweaking

How tight the clips become depends on your printer, printer settings and the rods you are using. base.test_rod_clamp_tightness() can be used to generate a test object with several clips. Edit Rods6mm_tightness in global_settings.inito change the fit globally.

Modifying the 3D parts

To modify the parts, you'll need to compile from Python to SCAD to STL:

To re-create all parts, run

python reference_assembly.py

Be aware that this might take a long time (more than an hour) depending on the resolution you choose in OpenSCAD. You can install the required python packages with pip3 install -r requirements.txt. Also, make sure you have OpenSCAD installed in the location specified in global_settings.ini

To get scad files:

  • A clone of this repository
  • Python (only tested for python 3)
  • Solidpython - usually available through pip install solidpython

To convert from scad to stl:

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Solidpython CAD files for a digital holographic microscope

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