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kmdouglass committed Sep 14, 2023
2 parents 68ab7d3 + cdeeeb0 commit b618d5c
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14 changes: 14 additions & 0 deletions texts/smlm-lab-course/chapters/lab-instructions.tex
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Expand Up @@ -75,15 +75,27 @@ \subsection{Turn On the Microscope}\label{sec:startup}

To turn on the microscope, turn on the following components in this order. There should be pieces of tape with numbers on them and attached to the actual devices to aid you.

\textbf{In the electronics cabinet:}

\begin{enumerate}
\item Laser engine
\item Laser safety key on the shutter controller
\item Scanner electronics: main switch
\item Scanner electronics: scanner
\end{enumerate}

\textbf{On the optical table:}

\begin{enumerate}
\item Camera \newline Wait for the status light to turn green before proceeding.
\item Olympus CBH microscope controller
\item Maerhaeuser Tango Desktop stage controller
\item Olympus control panel \newline Wait for the "Start Operation" button to appear, then press it to continue. This can take about one minute.
\end{enumerate}

\textbf{Back in the electronics cabinet:}

\begin{enumerate}
\item Laser shutter
\item The computer
\end{enumerate}
Expand Down Expand Up @@ -138,6 +150,8 @@ \subsubsection{Mount the Slide onto the Microscope}

Next, turn the focus knob so that the microscope objective is all the way down as indicated by the yellow line in the objective position indicator section of the microscope control panel. Then, place the slide into the sliding black metallic mounts and gently push them together until the slide is secure. Ensure that the coverslip is centered over the objective. Finally, turn the focus knob to raise the objective slowly until it is just touching the oil. You will know when the objective is touching the oil because you can see the oil spreading out underneath the objective. A video that illustrates this effect may be found at \url{https://drive.google.com/file/d/1IC5Azq-raW6_jVSnuBDrSsZ0OsQYxoPK/view?usp=sharing}.

When done, replace the black safety cover over the objective and sample.

\subsection{Turn Off the Microscope}

Turn off the components in the opposite order as in \autoref{sec:startup}.
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4 changes: 2 additions & 2 deletions texts/smlm-lab-course/smlm-lab-manual.tex
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Expand Up @@ -51,8 +51,6 @@ \section{Learning Outcomes}
\item Analyze data from the microscope to produce super-resolved images of cellular structures.
\end{enumerate}

\subfile{chapters/lab-Instructions}

\chapter{The Modern Fluorescence Microscope} \label{ch:microscopes}

\section{Epifluorescence Microscopes}
Expand Down Expand Up @@ -370,6 +368,8 @@ \subsection{Photoswitching vs Photoactivation}
\label{table:photophysics-comparison}
\end{table}

\subfile{chapters/lab-Instructions}

\bibliographystyle{plain}
\bibliography{refs}

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